Selected Cell
Cell:
Value:
Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
A
B
C
D
E
F
G
H
I
J
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
A
B
C
D
E
F
G
H
I
J
K
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
A
B
C
D
E
F
G
H
I
J
K
L
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
A
B
C
D
E
F
G
H
I
J
K
1801
1802
1803
1804
1805
1806
1807
1808
1809
1810
1811
1812
1813
1814
1815
1816
1817
1818
1819
1820
1821
1822
1823
1824
1825
1826
1827
1828
1829
1830
1831
1832
1833
1834
1835
1836
1837
1838
1839
1840
1841
1842
1843
1844
1845
1846
1847
1848
1849
1850
1851
1852
1853
1854
1855
1856
1857
1858
1859
1860
1861
1862
1863
1864
1865
1866
1867
1868
1869
1870
1871
1872
1873
1874
1875
1876
1877
1878
1879
1880
1881
1882
1883
1884
1885
1886
1887
1888
1889
1890
1891
1892
1893
1894
1895
1896
1897
1898
1899
1900
12.9456828523703 | 27.2121071815491 | 27.5139927864075 | 31.2274396419525 | 32.2219491004944 | 12.9456828523703 | 177.2121071815491 | 52.5139927864075 | 31.2274396419525 | 107.2219491004944 | |
12.9528828984506 | 27.2003829479218 | 27.5048494338989 | 31.2104821205139 | 32.2180211544037 | 12.9528828984506 | 177.2003829479218 | 52.5048494338989 | 31.2104821205139 | 107.2180211544037 | |
12.9600829445309 | 27.190500497818 | 27.4938344955445 | 31.1937332153321 | 32.2067975997925 | 12.9600829445309 | 177.190500497818 | 52.493834495544505 | 31.1937332153321 | 107.20679759979251 | |
12.9672829906112 | 27.1831572055817 | 27.480959892273 | 31.1793982982636 | 32.1892619132996 | 12.9672829906112 | 177.1831572055817 | 52.480959892273 | 31.1793982982636 | 107.1892619132996 | |
12.9744830366915 | 27.1784961223603 | 27.4662792682648 | 31.1692237854004 | 32.1668922901154 | 12.9744830366915 | 177.1784961223603 | 52.4662792682648 | 31.1692237854004 | 107.1668922901154 | |
12.9816830827718 | 27.1763145923615 | 27.4501025676728 | 31.1641573905945 | 32.1415364742279 | 12.9816830827718 | 177.1763145923615 | 52.4501025676728 | 31.1641573905945 | 107.1415364742279 | |
12.988883128852 | 27.1763145923615 | 27.4329364299774 | 31.164413690567 | 32.1150779724121 | 12.988883128852 | 177.1763145923615 | 52.4329364299774 | 31.164413690567 | 107.11507797241211 | |
12.9960831749323 | 27.1780133247376 | 27.4153888225556 | 31.1696529388428 | 32.0889592170716 | 12.9960831749323 | 177.1780133247376 | 52.4153888225556 | 31.1696529388428 | 107.08895921707159 | |
13.0032832210126 | 27.1805882453919 | 27.3982465267182 | 31.1791360378266 | 32.063752412796 | 13.0032832210126 | 177.1805882453919 | 52.3982465267182 | 31.1791360378266 | 107.06375241279599 | |
13.0104832670929 | 27.1828889846802 | 27.3826122283936 | 31.1918675899506 | 32.0391058921814 | 13.0104832670929 | 177.1828889846802 | 52.3826122283936 | 31.1918675899506 | 107.0391058921814 | |
13.0176833131732 | 27.1837711334229 | 27.3699522018433 | 31.2066018581391 | 32.0141553878784 | 13.0176833131732 | 177.1837711334229 | 52.369952201843304 | 31.2066018581391 | 107.01415538787839 | |
13.0248833592535 | 27.1825969219208 | 27.3617327213288 | 31.2219381332398 | 31.9881319999695 | 13.0248833592535 | 177.1825969219208 | 52.3617327213288 | 31.2219381332398 | 106.9881319999695 | |
13.0320834053338 | 27.1794080734253 | 27.3589134216309 | 31.2366127967835 | 31.9605827331543 | 13.0320834053338 | 177.1794080734253 | 52.3589134216309 | 31.2366127967835 | 106.9605827331543 | |
13.0392834514141 | 27.1746397018433 | 27.3613631725311 | 31.2496542930603 | 31.9315493106842 | 13.0392834514141 | 177.1746397018433 | 52.3613631725311 | 31.2496542930603 | 106.9315493106842 | |
13.0464834974944 | 27.1687686443329 | 27.3678123950958 | 31.2604188919068 | 31.9013416767121 | 13.0464834974944 | 177.16876864433289 | 52.3678123950958 | 31.2604188919068 | 106.9013416767121 | |
13.0536835435747 | 27.1620452404022 | 27.3763358592987 | 31.2685787677765 | 31.8704783916474 | 13.0536835435747 | 177.1620452404022 | 52.3763358592987 | 31.2685787677765 | 106.8704783916474 | |
13.060883589655 | 27.1545469760895 | 27.3847818374634 | 31.2741696834564 | 31.8397521972656 | 13.060883589655 | 177.1545469760895 | 52.3847818374634 | 31.2741696834564 | 106.8397521972656 | |
13.0680836357353 | 27.1463394165039 | 27.3911237716675 | 31.2776565551758 | 31.8099975585938 | 13.0680836357353 | 177.1463394165039 | 52.391123771667495 | 31.2776565551758 | 106.8099975585938 | |
13.0752836818156 | 27.1375060081482 | 27.39377617836 | 31.2797963619232 | 31.7816495895386 | 13.0752836818156 | 177.1375060081482 | 52.39377617836 | 31.2797963619232 | 106.7816495895386 | |
13.0824837278959 | 27.128005027771 | 27.3918867111206 | 31.2813043594361 | 31.754344701767 | 13.0824837278959 | 177.128005027771 | 52.3918867111206 | 31.2813043594361 | 106.754344701767 | |
13.0896837739762 | 27.1176099777222 | 27.3857057094574 | 31.2824010848999 | 31.7269206047058 | 13.0896837739762 | 177.1176099777222 | 52.3857057094574 | 31.2824010848999 | 106.7269206047058 | |
13.0968838200565 | 27.1061003208161 | 27.3766040802002 | 31.2827229499817 | 31.6978693008423 | 13.0968838200565 | 177.1061003208161 | 52.376604080200195 | 31.2827229499817 | 106.6978693008423 | |
13.1040838661368 | 27.0935118198395 | 27.3665964603424 | 31.2815189361572 | 31.6659867763519 | 13.1040838661368 | 177.0935118198395 | 52.3665964603424 | 31.2815189361572 | 106.6659867763519 | |
13.1112839122171 | 27.0803689956665 | 27.3576378822327 | 31.2778830528259 | 31.6305994987488 | 13.1112839122171 | 177.0803689956665 | 52.3576378822327 | 31.2778830528259 | 106.63059949874881 | |
13.1184839582974 | 27.0676374435425 | 27.3511588573456 | 31.2708497047425 | 31.5915644168854 | 13.1184839582974 | 177.0676374435425 | 52.351158857345595 | 31.2708497047425 | 106.5915644168854 | |
13.1256840043777 | 27.056485414505 | 27.347856760025 | 31.2593281269074 | 31.5492510795593 | 13.1256840043777 | 177.056485414505 | 52.347856760024996 | 31.2593281269074 | 106.5492510795593 | |
13.1328840504579 | 27.0480751991272 | 27.3476421833039 | 31.2422096729279 | 31.504625082016 | 13.1328840504579 | 177.0480751991272 | 52.347642183303904 | 31.2422096729279 | 106.50462508201599 | |
13.1400840965382 | 27.0432770252228 | 27.3496866226196 | 31.2188982963562 | 31.4590394496918 | 13.1400840965382 | 177.0432770252228 | 52.3496866226196 | 31.2188982963562 | 106.4590394496918 | |
13.1472841426185 | 27.0424544811249 | 27.3525476455689 | 31.1896860599518 | 31.4140379428864 | 13.1472841426185 | 177.0424544811249 | 52.352547645568904 | 31.1896860599518 | 106.4140379428864 | |
13.1544841886988 | 27.0453274250031 | 27.3545861244202 | 31.1557292938233 | 31.3707888126374 | 13.1544841886988 | 177.0453274250031 | 52.3545861244202 | 31.1557292938233 | 106.3707888126374 | |
13.1616842347791 | 27.0510315895081 | 27.3545503616333 | 31.1186134815216 | 31.329870223999 | 13.1616842347791 | 177.0510315895081 | 52.3545503616333 | 31.1186134815216 | 106.329870223999 | |
13.1688842808594 | 27.0581841468811 | 27.3518860340119 | 31.0798287391663 | 31.2913835048676 | 13.1688842808594 | 177.0581841468811 | 52.3518860340119 | 31.0798287391663 | 106.2913835048676 | |
13.1760843269397 | 27.0651519298554 | 27.3466885089874 | 31.0406267642975 | 31.2550008296967 | 13.1760843269397 | 177.0651519298554 | 52.3466885089874 | 31.0406267642975 | 106.2550008296967 | |
13.18328437302 | 27.070289850235 | 27.3393988609314 | 31.002277135849 | 31.2199950218201 | 13.18328437302 | 177.07028985023499 | 52.3393988609314 | 31.002277135849 | 106.2199950218201 | |
13.1904844191003 | 27.072149515152 | 27.3305654525757 | 30.9661149978638 | 31.1852991580963 | 13.1904844191003 | 177.072149515152 | 52.3305654525757 | 30.9661149978638 | 106.1852991580963 | |
13.1976844651806 | 27.0698964595795 | 27.3207902908325 | 30.9332609176636 | 31.1496675014496 | 13.1976844651806 | 177.0698964595795 | 52.320790290832505 | 30.9332609176636 | 106.1496675014496 | |
13.2048845112609 | 27.0634174346924 | 27.3107588291168 | 30.9043288230896 | 31.112003326416 | 13.2048845112609 | 177.0634174346924 | 52.3107588291168 | 30.9043288230896 | 106.112003326416 | |
13.2120845573412 | 27.0532786846161 | 27.3010551929474 | 30.8794021606446 | 31.0717284679413 | 13.2120845573412 | 177.0532786846161 | 52.3010551929474 | 30.8794021606446 | 106.0717284679413 | |
13.2192846034215 | 27.0404398441315 | 27.2919893264771 | 30.8583199977875 | 31.0288310050965 | 13.2192846034215 | 177.0404398441315 | 52.2919893264771 | 30.8583199977875 | 106.02883100509649 | |
13.2264846495018 | 27.0260691642761 | 27.2835493087769 | 30.8411359786988 | 30.9838831424713 | 13.2264846495018 | 177.0260691642761 | 52.2835493087769 | 30.8411359786988 | 105.9838831424713 | |
13.2336846955821 | 27.011239528656 | 27.2755563259125 | 30.8281004428864 | 30.9380471706391 | 13.2336846955821 | 177.011239528656 | 52.275556325912504 | 30.8281004428864 | 105.9380471706391 | |
13.2408847416624 | 26.9969522953034 | 27.2680282592774 | 30.8194041252136 | 30.8928906917572 | 13.2408847416624 | 176.9969522953034 | 52.2680282592774 | 30.8194041252136 | 105.8928906917572 | |
13.2480847877427 | 26.984041929245 | 27.2611320018769 | 30.815052986145 | 30.850237607956 | 13.2480847877427 | 176.984041929245 | 52.2611320018769 | 30.815052986145 | 105.850237607956 | |
13.255284833823 | 26.9732058048249 | 27.2550463676453 | 30.8149933815003 | 30.8119654655457 | 13.255284833823 | 176.9732058048249 | 52.2550463676453 | 30.8149933815003 | 105.8119654655457 | |
13.2624848799033 | 26.9649505615235 | 27.2496938705445 | 30.8194935321808 | 30.7797253131867 | 13.2624848799033 | 176.9649505615235 | 52.249693870544505 | 30.8194935321808 | 105.7797253131867 | |
13.2696849259835 | 26.9595444202423 | 27.2446393966675 | 30.8293282985687 | 30.75470328331 | 13.2696849259835 | 176.9595444202423 | 52.244639396667495 | 30.8293282985687 | 105.75470328331 | |
13.2768849720638 | 26.9569456577301 | 27.2391974925995 | 30.8458268642426 | 30.7375967502594 | 13.2768849720638 | 176.9569456577301 | 52.2391974925995 | 30.8458268642426 | 105.7375967502594 | |
13.2840850181441 | 26.9568264484406 | 27.2327363491059 | 30.8707714080811 | 30.7287454605103 | 13.2840850181441 | 176.9568264484406 | 52.2327363491059 | 30.8707714080811 | 105.7287454605103 | |
13.2912850642244 | 26.9587218761444 | 27.2247850894928 | 30.9064507484436 | 30.728429555893 | 13.2912850642244 | 176.9587218761444 | 52.2247850894928 | 30.9064507484436 | 105.728429555893 | |
13.2984851103047 | 26.9621789455414 | 27.2151529788971 | 30.9559166431427 | 30.7373225688935 | 13.2984851103047 | 176.9621789455414 | 52.215152978897095 | 30.9559166431427 | 105.7373225688935 | |
13.305685156385 | 26.9667744636536 | 27.2040605545044 | 31.0229241847992 | 30.7566463947296 | 13.305685156385 | 176.9667744636536 | 52.2040605545044 | 31.0229241847992 | 105.7566463947296 | |
13.3128852024653 | 26.9720017910004 | 27.192085981369 | 31.1115264892578 | 30.7880103588104 | 13.3128852024653 | 176.9720017910004 | 52.192085981369004 | 31.1115264892578 | 105.7880103588104 | |
13.3200852485456 | 26.9771039485932 | 27.1800994873047 | 31.225335597992 | 30.8330416679383 | 13.3200852485456 | 176.9771039485932 | 52.1800994873047 | 31.225335597992 | 105.8330416679383 | |
13.3272852946259 | 26.9813120365143 | 27.169132232666 | 31.3668072223664 | 30.8928847312927 | 13.3272852946259 | 176.9813120365143 | 52.169132232666 | 31.3668072223664 | 105.8928847312927 | |
13.3344853407062 | 26.9841313362122 | 27.1600604057312 | 31.536728143692 | 30.9676229953766 | 13.3344853407062 | 176.98413133621222 | 52.1600604057312 | 31.536728143692 | 105.9676229953766 | |
13.3416853867865 | 26.985377073288 | 27.1534323692322 | 31.7340016365051 | 31.0559451580048 | 13.3416853867865 | 176.985377073288 | 52.1534323692322 | 31.7340016365051 | 106.0559451580048 | |
13.3488854328668 | 26.9850432872772 | 27.1493315696717 | 31.9553077220917 | 31.1549365520478 | 13.3488854328668 | 176.9850432872772 | 52.1493315696717 | 31.9553077220917 | 106.1549365520478 | |
13.3560854789471 | 26.9829571247101 | 27.1474123001099 | 32.1947634220124 | 31.2601208686829 | 13.3560854789471 | 176.9829571247101 | 52.1474123001099 | 32.1947634220124 | 106.2601208686829 | |
13.3632855250274 | 26.9787549972534 | 27.1471917629242 | 32.4439644813538 | 31.3658237457276 | 13.3632855250274 | 176.9787549972534 | 52.1471917629242 | 32.4439644813538 | 106.3658237457276 | |
13.3704855711077 | 26.9723355770111 | 27.1482288837433 | 32.6925456523895 | 31.4657032489777 | 13.3704855711077 | 176.9723355770111 | 52.1482288837433 | 32.6925456523895 | 106.4657032489777 | |
13.377685617188 | 26.9641101360321 | 27.1502673625946 | 32.9292058944702 | 31.5534591674805 | 13.377685617188 | 176.9641101360321 | 52.150267362594604 | 32.9292058944702 | 106.5534591674805 | |
13.3848856632683 | 26.9550383090973 | 27.15322971344 | 33.1427931785584 | 31.6235899925232 | 13.3848856632683 | 176.9550383090973 | 52.15322971344 | 33.1427931785584 | 106.6235899925232 | |
13.3920857093486 | 26.9462525844574 | 27.1570324897766 | 33.3230972290039 | 31.6718757152558 | 13.3920857093486 | 176.9462525844574 | 52.1570324897766 | 33.3230972290039 | 106.6718757152558 | |
13.3992857554289 | 26.9387364387512 | 27.161568403244 | 33.4615230560303 | 31.6957831382752 | 13.3992857554289 | 176.9387364387512 | 52.161568403244004 | 33.4615230560303 | 106.6957831382752 | |
13.4064858015092 | 26.933240890503 | 27.1666407585144 | 33.5519671440125 | 31.6945970058441 | 13.4064858015092 | 176.933240890503 | 52.166640758514404 | 33.5519671440125 | 106.6945970058441 | |
13.4136858475894 | 26.9304573535919 | 27.1719515323639 | 33.5915625095368 | 31.66943192482 | 13.4136858475894 | 176.9304573535919 | 52.1719515323639 | 33.5915625095368 | 106.66943192482 | |
13.4208858936697 | 26.9309759140015 | 27.1769225597382 | 33.5811972618103 | 31.6231727600098 | 13.4208858936697 | 176.9309759140015 | 52.1769225597382 | 33.5811972618103 | 106.6231727600098 | |
13.42808593975 | 26.9350051879883 | 27.1809339523316 | 33.5252940654755 | 31.5602362155915 | 13.42808593975 | 176.9350051879883 | 52.1809339523316 | 33.5252940654755 | 106.5602362155915 | |
13.4352859858303 | 26.9421041011811 | 27.1835863590241 | 33.431226015091 | 31.4862787723541 | 13.4352859858303 | 176.9421041011811 | 52.183586359024105 | 33.431226015091 | 106.4862787723541 | |
13.4424860319106 | 26.9511282444 | 27.1850109100342 | 33.3086133003235 | 31.4078330993653 | 13.4424860319106 | 176.9511282444 | 52.1850109100342 | 33.3086133003235 | 106.40783309936529 | |
13.4496860779909 | 26.960563659668 | 27.1859526634217 | 33.1688463687897 | 31.3318192958832 | 13.4496860779909 | 176.960563659668 | 52.1859526634217 | 33.1688463687897 | 106.33181929588321 | |
13.4568861240712 | 26.9689559936524 | 27.1873712539673 | 33.02441239357 | 31.2650322914124 | 13.4568861240712 | 176.9689559936524 | 52.1873712539673 | 33.02441239357 | 106.2650322914124 | |
13.4640861701515 | 26.9751965999603 | 27.1898627281189 | 32.8881561756134 | 31.213515996933 | 13.4640861701515 | 176.9751965999603 | 52.1898627281189 | 32.8881561756134 | 106.213515996933 | |
13.4712862162318 | 26.9785284996033 | 27.193546295166 | 32.7722609043122 | 31.1820685863495 | 13.4712862162318 | 176.9785284996033 | 52.193546295166 | 32.7722609043122 | 106.1820685863495 | |
13.4784862623121 | 26.9785821437836 | 27.1981716156006 | 32.6873660087586 | 31.1739385128021 | 13.4784862623121 | 176.9785821437836 | 52.1981716156006 | 32.6873660087586 | 106.1739385128021 | |
13.4856863083924 | 26.9754827022553 | 27.2032499313355 | 32.641863822937 | 31.1905920505524 | 13.4856863083924 | 176.9754827022553 | 52.2032499313355 | 32.641863822937 | 106.1905920505524 | |
13.4928863544727 | 26.9699811935425 | 27.2080540657044 | 32.6415121555329 | 31.2316119670868 | 13.4928863544727 | 176.9699811935425 | 52.2080540657044 | 32.6415121555329 | 106.23161196708679 | |
13.500086400553 | 26.963472366333 | 27.2116005420685 | 32.6890110969544 | 31.2947988510132 | 13.500086400553 | 176.963472366333 | 52.211600542068496 | 32.6890110969544 | 106.2947988510132 | |
13.5072864466333 | 26.957631111145 | 27.2127628326416 | 32.783579826355 | 31.3763320446015 | 13.5072864466333 | 176.957631111145 | 52.2127628326416 | 32.783579826355 | 106.3763320446015 | |
13.5144864927136 | 26.9539892673493 | 27.2108376026154 | 32.9207301139832 | 31.4712047576905 | 13.5144864927136 | 176.9539892673493 | 52.2108376026154 | 32.9207301139832 | 106.4712047576905 | |
13.5216865387939 | 26.9536912441254 | 27.206027507782 | 33.0924987792969 | 31.5737187862396 | 13.5216865387939 | 176.9536912441254 | 52.206027507782 | 33.0924987792969 | 106.5737187862396 | |
13.5288865848742 | 26.9573986530304 | 27.1993517875672 | 33.2882225513458 | 31.6776931285858 | 13.5288865848742 | 176.9573986530304 | 52.199351787567196 | 33.2882225513458 | 106.6776931285858 | |
13.5360866309545 | 26.96533203125 | 27.1921575069428 | 33.495432138443 | 31.7767143249512 | 13.5360866309545 | 176.96533203125 | 52.1921575069428 | 33.495432138443 | 106.7767143249512 | |
13.5432866770348 | 26.9772708415985 | 27.1856188774109 | 33.7007641792298 | 31.8644225597382 | 13.5432866770348 | 176.9772708415985 | 52.1856188774109 | 33.7007641792298 | 106.8644225597382 | |
13.5504867231151 | 26.992404460907 | 27.1804392337799 | 33.8907599449158 | 31.9349348545075 | 13.5504867231151 | 176.992404460907 | 52.1804392337799 | 33.8907599449158 | 106.9349348545075 | |
13.5576867691953 | 27.0094275474549 | 27.1771252155304 | 34.0526998043061 | 31.9831728935242 | 13.5576867691953 | 177.0094275474549 | 52.177125215530396 | 34.0526998043061 | 106.9831728935242 | |
13.5648868152756 | 27.0268321037293 | 27.1761357784271 | 34.1756522655487 | 32.0051908493042 | 13.5648868152756 | 177.0268321037293 | 52.176135778427096 | 34.1756522655487 | 107.0051908493042 | |
13.5720868613559 | 27.0431101322174 | 27.1777391433716 | 34.2513263225556 | 31.9981813430786 | 13.5720868613559 | 177.0431101322174 | 52.177739143371596 | 34.2513263225556 | 106.9981813430786 | |
13.5792869074362 | 27.057009935379 | 27.1817922592163 | 34.2744827270508 | 31.9607496261597 | 13.5792869074362 | 177.057009935379 | 52.1817922592163 | 34.2744827270508 | 106.9607496261597 | |
13.5864869535165 | 27.0675420761109 | 27.1877229213715 | 34.24271941185 | 31.8931937217713 | 13.5864869535165 | 177.0675420761109 | 52.1877229213715 | 34.24271941185 | 106.8931937217713 | |
13.5936869995968 | 27.0740032196045 | 27.1947681903839 | 34.1561853885651 | 31.7976832389832 | 13.5936869995968 | 177.0740032196045 | 52.1947681903839 | 34.1561853885651 | 106.7976832389832 | |
13.6008870456771 | 27.0761370658875 | 27.2023975849152 | 34.0174198150635 | 31.6779613494873 | 13.6008870456771 | 177.0761370658875 | 52.202397584915204 | 34.0174198150635 | 106.6779613494873 | |
13.6080870917574 | 27.074271440506 | 27.2104680538178 | 33.8312864303589 | 31.5387547016144 | 13.6080870917574 | 177.074271440506 | 52.2104680538178 | 33.8312864303589 | 106.53875470161441 | |
13.6152871378377 | 27.0691812038422 | 27.2189199924469 | 33.6046755313874 | 31.3851833343506 | 13.6152871378377 | 177.0691812038422 | 52.2189199924469 | 33.6046755313874 | 106.3851833343506 | |
13.622487183918 | 27.0618140697479 | 27.2274315357208 | 33.345627784729 | 31.2223374843598 | 13.622487183918 | 177.0618140697479 | 52.2274315357208 | 33.345627784729 | 106.2223374843598 | |
13.6296872299983 | 27.0530760288239 | 27.2352933883667 | 33.0623984336853 | 31.0550391674042 | 13.6296872299983 | 177.0530760288239 | 52.2352933883667 | 33.0623984336853 | 106.0550391674042 | |
13.6368872760786 | 27.0436406135559 | 27.241587638855 | 32.7628910541535 | 30.8875560760498 | 13.6368872760786 | 177.0436406135559 | 52.241587638854995 | 32.7628910541535 | 105.8875560760498 | |
13.6440873221589 | 27.033942937851 | 27.2454202175141 | 32.4545979499817 | 30.7230949401856 | 13.6440873221589 | 177.033942937851 | 52.245420217514095 | 32.4545979499817 | 105.7230949401856 | |
13.6512873682392 | 27.0241975784302 | 27.2460460662842 | 32.1446001529694 | 30.563747882843 | 13.6512873682392 | 177.0241975784302 | 52.2460460662842 | 32.1446001529694 | 105.563747882843 | |
13.6584874143195 | 27.0143926143646 | 27.2428154945374 | 31.8392276763916 | 30.4106175899506 | 13.6584874143195 | 177.0143926143646 | 52.242815494537396 | 31.8392276763916 | 105.4106175899506 |