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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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12.2256782443408 | 27.3758471012116 | 27.3951709270478 | 29.3566644191742 | 29.5775473117829 | 12.2256782443408 | 177.3758471012116 | 52.3951709270478 | 29.3566644191742 | 104.5775473117829 | |
12.2328782904211 | 27.3638427257538 | 27.3979842662811 | 29.3462753295899 | 29.5739948749542 | 12.2328782904211 | 177.36384272575378 | 52.3979842662811 | 29.3462753295899 | 104.5739948749542 | |
12.2400783365014 | 27.3539364337921 | 27.4053573608399 | 29.3369591236115 | 29.5705556869507 | 12.2400783365014 | 177.35393643379211 | 52.4053573608399 | 29.3369591236115 | 104.5705556869507 | |
12.2472783825817 | 27.3476243019104 | 27.4168193340302 | 29.3290317058563 | 29.5674502849579 | 12.2472783825817 | 177.3476243019104 | 52.4168193340302 | 29.3290317058563 | 104.5674502849579 | |
12.254478428662 | 27.3458063602448 | 27.4311542510987 | 29.3228268623352 | 29.5649468898773 | 12.254478428662 | 177.3458063602448 | 52.431154251098704 | 29.3228268623352 | 104.5649468898773 | |
12.2616784747423 | 27.3486733436585 | 27.4465680122376 | 29.3185114860535 | 29.5632123947144 | 12.2616784747423 | 177.3486733436585 | 52.4465680122376 | 29.3185114860535 | 104.5632123947144 | |
12.2688785208226 | 27.3556530475617 | 27.4610102176666 | 29.3160200119019 | 29.5621871948242 | 12.2688785208226 | 177.3556530475617 | 52.4610102176666 | 29.3160200119019 | 104.5621871948242 | |
12.2760785669029 | 27.3653328418732 | 27.472734451294 | 29.3152093887329 | 29.5615196228028 | 12.2760785669029 | 177.3653328418732 | 52.472734451294 | 29.3152093887329 | 104.56151962280279 | |
12.2832786129831 | 27.3757576942444 | 27.4808764457703 | 29.3160736560822 | 29.5605063438416 | 12.2832786129831 | 177.37575769424438 | 52.4808764457703 | 29.3160736560822 | 104.5605063438416 | |
12.2904786590634 | 27.3849964141846 | 27.4854600429535 | 29.3186545372009 | 29.5581638813019 | 12.2904786590634 | 177.3849964141846 | 52.4854600429535 | 29.3186545372009 | 104.55816388130191 | |
12.2976787051437 | 27.3919463157654 | 27.4872362613678 | 29.3229579925537 | 29.5533657073975 | 12.2976787051437 | 177.3919463157654 | 52.4872362613678 | 29.3229579925537 | 104.5533657073975 | |
12.304878751224 | 27.3965358734131 | 27.4875700473786 | 29.3288230895996 | 29.5452117919922 | 12.304878751224 | 177.3965358734131 | 52.4875700473786 | 29.3288230895996 | 104.5452117919922 | |
12.3120787973043 | 27.3994326591492 | 27.4881362915039 | 29.3359339237213 | 29.5333564281464 | 12.3120787973043 | 177.3994326591492 | 52.4881362915039 | 29.3359339237213 | 104.5333564281464 | |
12.3192788433846 | 27.4015069007874 | 27.4905502796173 | 29.3440401554108 | 29.5181453227997 | 12.3192788433846 | 177.4015069007874 | 52.490550279617295 | 29.3440401554108 | 104.5181453227997 | |
12.3264788894649 | 27.403599023819 | 27.4959087371826 | 29.3530642986298 | 29.5006334781647 | 12.3264788894649 | 177.403599023819 | 52.4959087371826 | 29.3530642986298 | 104.5006334781647 | |
12.3336789355452 | 27.4065911769867 | 27.5043249130249 | 29.3629288673401 | 29.4823169708252 | 12.3336789355452 | 177.4065911769867 | 52.5043249130249 | 29.3629288673401 | 104.4823169708252 | |
12.3408789816255 | 27.4113893508911 | 27.5148987770081 | 29.3733477592468 | 29.4647336006165 | 12.3408789816255 | 177.4113893508911 | 52.5148987770081 | 29.3733477592468 | 104.4647336006165 | |
12.3480790277058 | 27.4184763431549 | 27.5261342525482 | 29.3837606906891 | 29.4491350650788 | 12.3480790277058 | 177.4184763431549 | 52.526134252548204 | 29.3837606906891 | 104.44913506507879 | |
12.3552790737861 | 27.4274587631226 | 27.5363981723786 | 29.3935537338257 | 29.4361829757691 | 12.3552790737861 | 177.42745876312262 | 52.5363981723786 | 29.3935537338257 | 104.4361829757691 | |
12.3624791198664 | 27.4369776248932 | 27.5444447994232 | 29.4022500514984 | 29.4259905815125 | 12.3624791198664 | 177.4369776248932 | 52.544444799423204 | 29.4022500514984 | 104.42599058151251 | |
12.3696791659467 | 27.4453639984131 | 27.5497198104859 | 29.4095993041992 | 29.4182062149048 | 12.3696791659467 | 177.4453639984131 | 52.5497198104859 | 29.4095993041992 | 104.4182062149048 | |
12.376879212027 | 27.4512827396393 | 27.5525808334351 | 29.4153690338135 | 29.4122457504273 | 12.376879212027 | 177.4512827396393 | 52.5525808334351 | 29.4153690338135 | 104.4122457504273 | |
12.3840792581073 | 27.4540901184082 | 27.5541245937348 | 29.4192492961884 | 29.4073224067688 | 12.3840792581073 | 177.4540901184082 | 52.5541245937348 | 29.4192492961884 | 104.4073224067688 | |
12.3912793041876 | 27.4535953998566 | 27.5558114051819 | 29.4210076332092 | 29.4025957584381 | 12.3912793041876 | 177.4535953998566 | 52.5558114051819 | 29.4210076332092 | 104.4025957584381 | |
12.3984793502679 | 27.4497330188751 | 27.5587797164917 | 29.4205486774445 | 29.3973743915558 | 12.3984793502679 | 177.4497330188751 | 52.5587797164917 | 29.4205486774445 | 104.3973743915558 | |
12.4056793963482 | 27.4425208568573 | 27.5635123252869 | 29.4179320335388 | 29.3915212154389 | 12.4056793963482 | 177.4425208568573 | 52.5635123252869 | 29.4179320335388 | 104.3915212154389 | |
12.4128794424285 | 27.4321973323822 | 27.5696337223053 | 29.4132709503174 | 29.3856263160706 | 12.4128794424285 | 177.4321973323822 | 52.5696337223053 | 29.4132709503174 | 104.3856263160706 | |
12.4200794885087 | 27.4193286895752 | 27.576094865799 | 29.4065415859223 | 29.3808221817017 | 12.4200794885087 | 177.4193286895752 | 52.576094865799 | 29.4065415859223 | 104.3808221817017 | |
12.427279534589 | 27.4045825004578 | 27.5813698768616 | 29.3976664543152 | 29.3784737586975 | 12.427279534589 | 177.4045825004578 | 52.5813698768616 | 29.3976664543152 | 104.3784737586975 | |
12.4344795806693 | 27.3886382579804 | 27.5838613510132 | 29.3866395950318 | 29.3797969818115 | 12.4344795806693 | 177.3886382579804 | 52.5838613510132 | 29.3866395950318 | 104.3797969818115 | |
12.4416796267496 | 27.3721218109131 | 27.5823533535004 | 29.3736457824707 | 29.3857276439667 | 12.4416796267496 | 177.3721218109131 | 52.5823533535004 | 29.3736457824707 | 104.3857276439667 | |
12.4488796728299 | 27.355694770813 | 27.5765180587769 | 29.3593227863312 | 29.396915435791 | 12.4488796728299 | 177.355694770813 | 52.5765180587769 | 29.3593227863312 | 104.396915435791 | |
12.4560797189102 | 27.340179681778 | 27.5671243667603 | 29.3449342250824 | 29.4135928153992 | 12.4560797189102 | 177.340179681778 | 52.5671243667603 | 29.3449342250824 | 104.4135928153992 | |
12.4632797649905 | 27.3265421390534 | 27.5556683540344 | 29.3322622776032 | 29.4353425502777 | 12.4632797649905 | 177.3265421390534 | 52.555668354034395 | 29.3322622776032 | 104.4353425502777 | |
12.4704798110708 | 27.3157238960266 | 27.5438368320465 | 29.3233990669251 | 29.4611036777497 | 12.4704798110708 | 177.3157238960266 | 52.5438368320465 | 29.3233990669251 | 104.4611036777497 | |
12.4776798571511 | 27.3084223270416 | 27.5330722332001 | 29.3202519416809 | 29.4893383979798 | 12.4776798571511 | 177.3084223270416 | 52.5330722332001 | 29.3202519416809 | 104.4893383979798 | |
12.4848799032314 | 27.3048758506775 | 27.5243878364563 | 29.3241024017334 | 29.5184314250946 | 12.4848799032314 | 177.3048758506775 | 52.5243878364563 | 29.3241024017334 | 104.5184314250946 | |
12.4920799493117 | 27.304619550705 | 27.5182664394379 | 29.3355107307434 | 29.5471489429474 | 12.4920799493117 | 177.304619550705 | 52.5182664394379 | 29.3355107307434 | 104.5471489429474 | |
12.499279995392 | 27.306592464447 | 27.5145649909973 | 29.3543040752411 | 29.5748710632324 | 12.499279995392 | 177.306592464447 | 52.5145649909973 | 29.3543040752411 | 104.5748710632324 | |
12.5064800414723 | 27.3093521595001 | 27.5123953819275 | 29.3796896934509 | 29.6017050743103 | 12.5064800414723 | 177.3093521595001 | 52.512395381927504 | 29.3796896934509 | 104.6017050743103 | |
12.5136800875526 | 27.3114919662476 | 27.510553598404 | 29.4102966785431 | 29.6284794807434 | 12.5136800875526 | 177.31149196624762 | 52.510553598404 | 29.4102966785431 | 104.62847948074341 | |
12.5208801336329 | 27.3119568824768 | 27.5080621242523 | 29.4443964958191 | 29.6566069126129 | 12.5208801336329 | 177.3119568824768 | 52.508062124252305 | 29.4443964958191 | 104.6566069126129 | |
12.5280801797132 | 27.3101210594177 | 27.5045573711395 | 29.480242729187 | 29.6878457069397 | 12.5280801797132 | 177.3101210594177 | 52.5045573711395 | 29.480242729187 | 104.6878457069397 | |
12.5352802257935 | 27.3058652877808 | 27.5003015995026 | 29.5166611671448 | 29.7240793704987 | 12.5352802257935 | 177.3058652877808 | 52.5003015995026 | 29.5166611671448 | 104.7240793704987 | |
12.5424802718738 | 27.2997081279755 | 27.4959206581116 | 29.5534193515778 | 29.7671377658844 | 12.5424802718738 | 177.2997081279755 | 52.4959206581116 | 29.5534193515778 | 104.7671377658844 | |
12.5496803179541 | 27.2928178310394 | 27.4922847747803 | 29.5912384986878 | 29.8186361789704 | 12.5496803179541 | 177.2928178310394 | 52.4922847747803 | 29.5912384986878 | 104.8186361789704 | |
12.5568803640343 | 27.2869646549225 | 27.4904668331146 | 29.6314418315888 | 29.8796951770783 | 12.5568803640343 | 177.28696465492249 | 52.490466833114596 | 29.6314418315888 | 104.8796951770783 | |
12.5640804101146 | 27.2841215133667 | 27.4915635585785 | 29.6755075454712 | 29.9506068229676 | 12.5640804101146 | 177.2841215133667 | 52.4915635585785 | 29.6755075454712 | 104.9506068229676 | |
12.5712804561949 | 27.28590965271 | 27.4963259696961 | 29.7248840332031 | 30.030757188797 | 12.5712804561949 | 177.28590965271 | 52.4963259696961 | 29.7248840332031 | 105.030757188797 | |
12.5784805022752 | 27.2933661937714 | 27.5049924850464 | 29.780912399292 | 30.1188349723816 | 12.5784805022752 | 177.2933661937714 | 52.5049924850464 | 29.780912399292 | 105.11883497238159 | |
12.5856805483555 | 27.3068606853485 | 27.5173127651215 | 29.8447012901306 | 30.2131414413452 | 12.5856805483555 | 177.3068606853485 | 52.5173127651215 | 29.8447012901306 | 105.2131414413452 | |
12.5928805944358 | 27.3261904716492 | 27.5326609611511 | 29.9168288707733 | 30.3117215633393 | 12.5928805944358 | 177.3261904716492 | 52.5326609611511 | 29.9168288707733 | 105.3117215633393 | |
12.6000806405161 | 27.3505985736847 | 27.550208568573 | 29.9970209598541 | 30.412220954895 | 12.6000806405161 | 177.3505985736847 | 52.550208568573 | 29.9970209598541 | 105.412220954895 | |
12.6072806865964 | 27.3786544799805 | 27.5690257549286 | 30.0842165946961 | 30.5118620395661 | 12.6072806865964 | 177.3786544799805 | 52.5690257549286 | 30.0842165946961 | 105.5118620395661 | |
12.6144807326767 | 27.4084329605103 | 27.5882840156555 | 30.1768481731415 | 30.6077241897583 | 12.6144807326767 | 177.4084329605103 | 52.5882840156555 | 30.1768481731415 | 105.6077241897583 | |
12.621680778757 | 27.4380385875702 | 27.6075184345246 | 30.2730858325958 | 30.6973159313202 | 12.621680778757 | 177.4380385875702 | 52.6075184345246 | 30.2730858325958 | 105.6973159313202 | |
12.6288808248373 | 27.4660050868988 | 27.6265501976013 | 30.3709328174591 | 30.778831243515 | 12.6288808248373 | 177.4660050868988 | 52.626550197601304 | 30.3709328174591 | 105.778831243515 | |
12.6360808709176 | 27.4914860725403 | 27.6452541351319 | 30.4682195186615 | 30.8510601520539 | 12.6360808709176 | 177.4914860725403 | 52.6452541351319 | 30.4682195186615 | 105.8510601520539 | |
12.6432809169979 | 27.5139272212982 | 27.6633083820343 | 30.5625677108765 | 30.9130311012268 | 12.6432809169979 | 177.5139272212982 | 52.6633083820343 | 30.5625677108765 | 105.9130311012268 | |
12.6504809630782 | 27.5328099727631 | 27.6801943778992 | 30.6513905525208 | 30.9638857841492 | 12.6504809630782 | 177.5328099727631 | 52.6801943778992 | 30.6513905525208 | 105.9638857841492 | |
12.6576810091585 | 27.5477349758148 | 27.6952028274536 | 30.7320415973664 | 31.0031473636627 | 12.6576810091585 | 177.5477349758148 | 52.6952028274536 | 30.7320415973664 | 106.00314736366269 | |
12.6648810552388 | 27.5586485862732 | 27.7073800563812 | 30.801796913147 | 31.0308039188385 | 12.6648810552388 | 177.5586485862732 | 52.7073800563812 | 30.801796913147 | 106.0308039188385 | |
12.6720811013191 | 27.5656819343567 | 27.7154445648194 | 30.8582007884979 | 31.0471475124359 | 12.6720811013191 | 177.5656819343567 | 52.7154445648194 | 30.8582007884979 | 106.0471475124359 | |
12.6792811473994 | 27.5687158107758 | 27.7179777622223 | 30.8992624282837 | 31.0524821281433 | 12.6792811473994 | 177.5687158107758 | 52.717977762222304 | 30.8992624282837 | 106.0524821281433 | |
12.6864811934797 | 27.5672197341919 | 27.7139544487 | 30.9236586093903 | 31.0470640659332 | 12.6864811934797 | 177.5672197341919 | 52.7139544487 | 30.9236586093903 | 106.0470640659332 | |
12.69368123956 | 27.5605201721192 | 27.7031540870667 | 30.9308230876923 | 31.03147149086 | 12.69368123956 | 177.5605201721192 | 52.7031540870667 | 30.9308230876923 | 106.03147149086 | |
12.7008812856402 | 27.5484621524811 | 27.686220407486 | 30.9210479259491 | 31.0068249702454 | 12.7008812856402 | 177.5484621524811 | 52.686220407486005 | 30.9210479259491 | 106.0068249702454 | |
12.7080813317205 | 27.5316953659058 | 27.6645362377167 | 30.8955788612366 | 30.9748351573944 | 12.7080813317205 | 177.5316953659058 | 52.6645362377167 | 30.8955788612366 | 105.9748351573944 | |
12.7152813778008 | 27.5113344192505 | 27.6399731636048 | 30.8565974235535 | 30.937522649765 | 12.7152813778008 | 177.5113344192505 | 52.6399731636048 | 30.8565974235535 | 105.937522649765 | |
12.7224814238811 | 27.4885892868042 | 27.6147782802582 | 30.8067858219147 | 30.8970451354981 | 12.7224814238811 | 177.4885892868042 | 52.6147782802582 | 30.8067858219147 | 105.8970451354981 | |
12.7296814699614 | 27.4647235870362 | 27.5912702083588 | 30.7487308979035 | 30.8557510375977 | 12.7296814699614 | 177.4647235870362 | 52.5912702083588 | 30.7487308979035 | 105.8557510375977 | |
12.7368815160417 | 27.4411857128144 | 27.5714218616486 | 30.684643983841 | 30.8160722255707 | 12.7368815160417 | 177.4411857128144 | 52.5714218616486 | 30.684643983841 | 105.81607222557071 | |
12.744081562122 | 27.4194955825806 | 27.556574344635 | 30.6165099143982 | 30.7802498340607 | 12.744081562122 | 177.4194955825806 | 52.556574344634996 | 30.6165099143982 | 105.7802498340607 | |
12.7512816082023 | 27.4007558822632 | 27.5473535060883 | 30.5463254451752 | 30.7501256465912 | 12.7512816082023 | 177.4007558822632 | 52.5473535060883 | 30.5463254451752 | 105.7501256465912 | |
12.7584816542826 | 27.3852348327637 | 27.5436997413635 | 30.4762423038483 | 30.7271718978882 | 12.7584816542826 | 177.3852348327637 | 52.5436997413635 | 30.4762423038483 | 105.7271718978882 | |
12.7656817003629 | 27.3725509643555 | 27.5448977947235 | 30.4084479808808 | 30.7127177715302 | 12.7656817003629 | 177.3725509643555 | 52.5448977947235 | 30.4084479808808 | 105.71271777153021 | |
12.7728817464432 | 27.3621618747711 | 27.5495290756226 | 30.345231294632 | 30.7080984115601 | 12.7728817464432 | 177.3621618747711 | 52.5495290756226 | 30.345231294632 | 105.7080984115601 | |
12.7800817925235 | 27.3536026477814 | 27.5556743144989 | 30.2893161773682 | 30.7145833969116 | 12.7800817925235 | 177.3536026477814 | 52.5556743144989 | 30.2893161773682 | 105.71458339691159 | |
12.7872818386038 | 27.3464441299439 | 27.5612950325012 | 30.2439332008362 | 30.7332515716553 | 12.7872818386038 | 177.3464441299439 | 52.5612950325012 | 30.2439332008362 | 105.7332515716553 | |
12.7944818846841 | 27.3401379585266 | 27.5646209716797 | 30.2125453948975 | 30.7650625705719 | 12.7944818846841 | 177.3401379585266 | 52.5646209716797 | 30.2125453948975 | 105.7650625705719 | |
12.8016819307644 | 27.3341953754425 | 27.5644659996033 | 30.1981568336487 | 30.8108747005463 | 12.8016819307644 | 177.3341953754425 | 52.5644659996033 | 30.1981568336487 | 105.8108747005463 | |
12.8088819768447 | 27.328485250473 | 27.5602757930756 | 30.202853679657 | 30.8712363243103 | 12.8088819768447 | 177.328485250473 | 52.560275793075604 | 30.202853679657 | 105.8712363243103 | |
12.816082022925 | 27.3232579231262 | 27.5521576404572 | 30.2275061607361 | 30.94602227211 | 12.816082022925 | 177.3232579231262 | 52.552157640457196 | 30.2275061607361 | 105.94602227211 | |
12.8232820690053 | 27.3188531398773 | 27.5409758090973 | 30.271863937378 | 31.034255027771 | 12.8232820690053 | 177.3188531398773 | 52.540975809097304 | 30.271863937378 | 106.034255027771 | |
12.8304821150856 | 27.3153305053711 | 27.5282442569733 | 30.3344607353211 | 31.1339974403382 | 12.8304821150856 | 177.3153305053711 | 52.528244256973295 | 30.3344607353211 | 106.13399744033819 | |
12.8376821611659 | 27.3124396800995 | 27.5157630443573 | 30.4127275943756 | 31.2425792217255 | 12.8376821611659 | 177.3124396800995 | 52.5157630443573 | 30.4127275943756 | 106.24257922172549 | |
12.8448822072461 | 27.3098468780518 | 27.505224943161 | 30.5032014846802 | 31.3565969467163 | 12.8448822072461 | 177.30984687805181 | 52.505224943161 | 30.5032014846802 | 106.35659694671631 | |
12.8520822533264 | 27.307277917862 | 27.4978399276734 | 30.6018948554993 | 31.4721643924713 | 12.8520822533264 | 177.307277917862 | 52.4978399276734 | 30.6018948554993 | 106.4721643924713 | |
12.8592822994067 | 27.3045241832733 | 27.494215965271 | 30.7045042514801 | 31.5853178501129 | 12.8592822994067 | 177.30452418327332 | 52.494215965270996 | 30.7045042514801 | 106.5853178501129 | |
12.866482345487 | 27.301424741745 | 27.4943709373474 | 30.8066725730896 | 31.6925406455994 | 12.866482345487 | 177.301424741745 | 52.4943709373474 | 30.8066725730896 | 106.6925406455994 | |
12.8736823915673 | 27.2977948188782 | 27.4977028369904 | 30.9042096138001 | 31.7911326885224 | 12.8736823915673 | 177.2977948188782 | 52.4977028369904 | 30.9042096138001 | 106.7911326885224 | |
12.8808824376476 | 27.2934973239899 | 27.5031685829163 | 30.9934556484223 | 31.8792879581452 | 12.8808824376476 | 177.2934973239899 | 52.5031685829163 | 30.9934556484223 | 106.8792879581452 | |
12.8880824837279 | 27.2883534431458 | 27.5095641613007 | 31.0716092586518 | 31.9560527801514 | 12.8880824837279 | 177.2883534431458 | 52.5095641613007 | 31.0716092586518 | 106.9560527801514 | |
12.8952825298082 | 27.2822201251984 | 27.5159180164337 | 31.1367213726044 | 32.0213437080384 | 12.8952825298082 | 177.2822201251984 | 52.5159180164337 | 31.1367213726044 | 107.0213437080384 | |
12.9024825758885 | 27.275025844574 | 27.5215566158295 | 31.1875641345978 | 32.075846195221 | 12.9024825758885 | 177.275025844574 | 52.521556615829496 | 31.1875641345978 | 107.075846195221 | |
12.9096826219688 | 27.2668421268463 | 27.525943517685 | 31.2236070632935 | 32.1207284927368 | 12.9096826219688 | 177.2668421268463 | 52.525943517685 | 31.2236070632935 | 107.1207284927368 | |
12.9168826680491 | 27.2577524185181 | 27.5285065174103 | 31.2452375888825 | 32.1570634841919 | 12.9168826680491 | 177.2577524185181 | 52.5285065174103 | 31.2452375888825 | 107.1570634841919 | |
12.9240827141294 | 27.2477209568024 | 27.5287032127381 | 31.2538683414459 | 32.1853935718537 | 12.9240827141294 | 177.2477209568024 | 52.5287032127381 | 31.2538683414459 | 107.1853935718537 | |
12.9312827602097 | 27.2366285324097 | 27.5262355804444 | 31.2518954277039 | 32.2057604789734 | 12.9312827602097 | 177.2366285324097 | 52.5262355804444 | 31.2518954277039 | 107.2057604789734 | |
12.93848280629 | 27.2245466709137 | 27.5212168693543 | 31.2421381473541 | 32.2179555892945 | 12.93848280629 | 177.2245466709137 | 52.521216869354305 | 31.2421381473541 | 107.21795558929449 |