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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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11.5056736363113 | 165.068113803864 | 33.7158679962158 | 77.4866998195648 | 128.885608911515 | 11.5056736363113 | 315.068113803864 | 58.7158679962158 | 77.4866998195648 | 203.885608911515 | |
11.5128736823916 | 159.894466400147 | 34.036135673523 | 76.5612840652466 | 125.191199779511 | 11.5128736823916 | 309.894466400147 | 59.036135673523 | 76.5612840652466 | 200.191199779511 | |
11.5200737284719 | 153.828090429306 | 34.3171119689942 | 75.2644777297974 | 120.848941802979 | 11.5200737284719 | 303.82809042930603 | 59.3171119689942 | 75.2644777297974 | 195.848941802979 | |
11.5272737745522 | 147.042268514633 | 34.5523297786713 | 73.6360907554627 | 115.984493494034 | 11.5272737745522 | 297.042268514633 | 59.5523297786713 | 73.6360907554627 | 190.98449349403398 | |
11.5344738206325 | 139.718163013458 | 34.7369849681854 | 71.7233240604401 | 110.727232694626 | 11.5344738206325 | 289.718163013458 | 59.7369849681854 | 71.7233240604401 | 185.727232694626 | |
11.5416738667128 | 132.037717103958 | 34.8679542541504 | 69.5785284042359 | 105.205404758454 | 11.5416738667128 | 282.037717103958 | 59.8679542541504 | 69.5785284042359 | 180.205404758454 | |
11.5488739127931 | 124.176269769669 | 34.9432826042176 | 67.2568559646607 | 99.5427370071411 | 11.5488739127931 | 274.17626976966903 | 59.9432826042176 | 67.2568559646607 | 174.5427370071411 | |
11.5560739588734 | 116.29501581192 | 34.9619090557099 | 64.8141503334046 | 93.8546419143677 | 11.5560739588734 | 266.29501581192 | 59.9619090557099 | 64.8141503334046 | 168.8546419143677 | |
11.5632740049537 | 108.534866571427 | 34.9240064620972 | 62.3050034046173 | 88.2448256015778 | 11.5632740049537 | 258.534866571427 | 59.9240064620972 | 62.3050034046173 | 163.24482560157782 | |
11.5704740510339 | 101.013386249542 | 34.8313927650452 | 59.7810983657837 | 82.8026711940766 | 11.5704740510339 | 251.013386249542 | 59.8313927650452 | 59.7810983657837 | 157.8026711940766 | |
11.5776740971142 | 93.824851512909 | 34.6876502037049 | 57.2896599769593 | 77.6019394397736 | 11.5776740971142 | 243.824851512909 | 59.6876502037049 | 57.2896599769593 | 152.60193943977362 | |
11.5848741431945 | 87.0414316654206 | 34.4975292682648 | 54.8720836639405 | 72.7006673812867 | 11.5848741431945 | 237.0414316654206 | 59.4975292682648 | 54.8720836639405 | 147.7006673812867 | |
11.5920741892748 | 80.7145118713379 | 34.2663407325745 | 52.5630593299866 | 68.1417763233185 | 11.5920741892748 | 230.7145118713379 | 59.2663407325745 | 52.5630593299866 | 143.14177632331848 | |
11.5992742353551 | 74.8761296272278 | 33.9998066425324 | 50.3902554512024 | 63.9534115791321 | 11.5992742353551 | 224.87612962722778 | 58.9998066425324 | 50.3902554512024 | 138.9534115791321 | |
11.6064742814354 | 69.5412635803223 | 33.704149723053 | 48.374605178833 | 60.1496875286103 | 11.6064742814354 | 219.5412635803223 | 58.704149723053 | 48.374605178833 | 135.1496875286103 | |
11.6136743275157 | 64.7109806537628 | 33.3860516548157 | 46.5309619903565 | 56.7322194576264 | 11.6136743275157 | 214.71098065376282 | 58.3860516548157 | 46.5309619903565 | 131.7322194576264 | |
11.620874373596 | 60.3752255439759 | 33.0522239208222 | 44.8685944080353 | 53.6920130252838 | 11.620874373596 | 210.3752255439759 | 58.0522239208222 | 44.8685944080353 | 128.6920130252838 | |
11.6280744196763 | 56.5142989158631 | 32.7090203762055 | 43.3916509151459 | 51.0120034217835 | 11.6280744196763 | 206.5142989158631 | 57.7090203762055 | 43.3916509151459 | 126.0120034217835 | |
11.6352744657566 | 53.1000852584839 | 32.3622822761536 | 42.0997619628906 | 48.669695854187 | 11.6352744657566 | 203.1000852584839 | 57.3622822761536 | 42.0997619628906 | 123.669695854187 | |
11.6424745118369 | 50.0978648662567 | 32.017469406128 | 40.9892141819 | 46.6395676136017 | 11.6424745118369 | 200.0978648662567 | 57.017469406128 | 40.9892141819 | 121.6395676136017 | |
11.6496745579172 | 47.4690735340119 | 31.6796481609345 | 40.0542318820954 | 44.8950469493866 | 11.6496745579172 | 197.4690735340119 | 56.679648160934505 | 40.0542318820954 | 119.8950469493866 | |
11.6568746039975 | 45.1742172241211 | 31.3533127307892 | 39.2881274223328 | 43.4098482131958 | 11.6568746039975 | 195.1742172241211 | 56.3533127307892 | 39.2881274223328 | 118.4098482131958 | |
11.6640746500778 | 43.1748747825623 | 31.042218208313 | 38.6837184429169 | 42.1586275100708 | 11.6640746500778 | 193.1748747825623 | 56.042218208313 | 38.6837184429169 | 117.1586275100708 | |
11.6712746961581 | 41.4348125457764 | 30.7493686676026 | 38.2333993911743 | 41.1175191402436 | 11.6712746961581 | 191.4348125457764 | 55.749368667602596 | 38.2333993911743 | 116.1175191402436 | |
11.6784747422384 | 39.9206459522248 | 30.4769217967987 | 37.9290759563446 | 40.2645885944367 | 11.6784747422384 | 189.9206459522248 | 55.4769217967987 | 37.9290759563446 | 115.2645885944367 | |
11.6856747883187 | 38.6025369167328 | 30.2261233329773 | 37.7621471881867 | 39.5799994468689 | 11.6856747883187 | 188.6025369167328 | 55.226123332977295 | 37.7621471881867 | 114.5799994468689 | |
11.692874834399 | 37.4543368816376 | 29.9970805644989 | 37.7234339714051 | 39.0456736087799 | 11.692874834399 | 187.4543368816376 | 54.9970805644989 | 37.7234339714051 | 114.04567360877991 | |
11.7000748804793 | 36.4534258842468 | 29.7888159751892 | 37.802928686142 | 38.644689321518 | 11.7000748804793 | 186.4534258842468 | 54.788815975189195 | 37.802928686142 | 113.644689321518 | |
11.7072749265596 | 35.5800688266754 | 29.5995354652405 | 37.9893481731415 | 38.3608639240265 | 11.7072749265596 | 185.58006882667542 | 54.5995354652405 | 37.9893481731415 | 113.3608639240265 | |
11.7144749726398 | 34.8168611526489 | 29.4269621372223 | 38.2696092128754 | 38.1788074970246 | 11.7144749726398 | 184.8168611526489 | 54.426962137222304 | 38.2696092128754 | 113.1788074970246 | |
11.7216750187201 | 34.1484487056732 | 29.2686402797699 | 38.6285781860352 | 38.0840003490448 | 11.7216750187201 | 184.1484487056732 | 54.2686402797699 | 38.6285781860352 | 113.0840003490448 | |
11.7288750648004 | 33.5614621639252 | 29.1222095489502 | 39.0491485595703 | 38.0622267723084 | 11.7288750648004 | 183.5614621639252 | 54.122209548950195 | 39.0491485595703 | 113.0622267723084 | |
11.7360751108807 | 33.0442845821381 | 28.9857387542725 | 39.5127058029175 | 38.0987346172333 | 11.7360751108807 | 183.0442845821381 | 53.985738754272504 | 39.5127058029175 | 113.0987346172333 | |
11.743275156961 | 32.5867533683777 | 28.8579106330872 | 39.9995565414429 | 38.1777346134186 | 11.743275156961 | 182.58675336837769 | 53.8579106330872 | 39.9995565414429 | 113.17773461341861 | |
11.7504752030413 | 32.1799576282501 | 28.7381231784821 | 40.4893517494202 | 38.2827818393707 | 11.7504752030413 | 182.1799576282501 | 53.7381231784821 | 40.4893517494202 | 113.2827818393707 | |
11.7576752491216 | 31.8160712718964 | 28.6262750625611 | 40.961354970932 | 38.3974611759186 | 11.7576752491216 | 181.8160712718964 | 53.6262750625611 | 40.961354970932 | 113.39746117591861 | |
11.7648752952019 | 31.4883649349213 | 28.522402048111 | 41.3949906826019 | 38.5058999061585 | 11.7648752952019 | 181.4883649349213 | 53.522402048111005 | 41.3949906826019 | 113.5058999061585 | |
11.7720753412822 | 31.1911106109619 | 28.4265220165253 | 41.770875453949 | 38.5928928852082 | 11.7720753412822 | 181.1911106109619 | 53.4265220165253 | 41.770875453949 | 113.5928928852082 | |
11.7792753873625 | 30.9195578098297 | 28.3385157585144 | 42.0717656612396 | 38.644278049469 | 11.7792753873625 | 180.9195578098297 | 53.338515758514404 | 42.0717656612396 | 113.644278049469 | |
11.7864754334428 | 30.6699693202973 | 28.2580077648163 | 42.2832131385803 | 38.6480867862702 | 11.7864754334428 | 180.6699693202973 | 53.2580077648163 | 42.2832131385803 | 113.6480867862702 | |
11.7936754795231 | 30.4395914077759 | 28.1843781471253 | 42.3937141895294 | 38.5957658290863 | 11.7936754795231 | 180.4395914077759 | 53.1843781471253 | 42.3937141895294 | 113.5957658290863 | |
11.8008755256034 | 30.2264213562012 | 28.1168639659882 | 42.3948407173157 | 38.4826242923737 | 11.8008755256034 | 180.2264213562012 | 53.1168639659882 | 42.3948407173157 | 113.4826242923737 | |
11.8080755716837 | 30.0289034843445 | 28.0548095703125 | 42.2817170619965 | 38.3073270320893 | 11.8080755716837 | 180.0289034843445 | 53.0548095703125 | 42.2817170619965 | 113.30732703208929 | |
11.815275617764 | 29.8457324504853 | 27.9981195926666 | 42.0537054538727 | 38.0711078643799 | 11.815275617764 | 179.8457324504853 | 52.9981195926666 | 42.0537054538727 | 113.0711078643799 | |
11.8224756638443 | 29.675817489624 | 27.9473721981049 | 41.7146980762482 | 37.7774655818939 | 11.8224756638443 | 179.675817489624 | 52.9473721981049 | 41.7146980762482 | 112.77746558189389 | |
11.8296757099246 | 29.5183897018433 | 27.9036402702332 | 41.2728428840637 | 37.4323666095734 | 11.8296757099246 | 179.5183897018433 | 52.9036402702332 | 41.2728428840637 | 112.43236660957339 | |
11.8368757560049 | 29.3727517127991 | 27.8679251670838 | 40.7398223876953 | 37.0441257953644 | 11.8368757560049 | 179.3727517127991 | 52.8679251670838 | 40.7398223876953 | 112.04412579536441 | |
11.8440758020852 | 29.2380511760712 | 27.8406322002411 | 40.1302039623261 | 36.6226732730866 | 11.8440758020852 | 179.2380511760712 | 52.8406322002411 | 40.1302039623261 | 111.6226732730866 | |
11.8512758481655 | 29.1132211685181 | 27.8213739395142 | 39.4608199596405 | 36.1783862113953 | 11.8512758481655 | 179.1132211685181 | 52.8213739395142 | 39.4608199596405 | 111.17838621139529 | |
11.8584758942457 | 28.9973199367523 | 27.8090715408325 | 38.7501537799835 | 35.7213735580445 | 11.8584758942457 | 178.9973199367523 | 52.809071540832505 | 38.7501537799835 | 110.72137355804449 | |
11.865675940326 | 28.889799118042 | 27.8020679950714 | 38.0172193050385 | 35.2612316608429 | 11.865675940326 | 178.889799118042 | 52.8020679950714 | 38.0172193050385 | 110.2612316608429 | |
11.8728759864063 | 28.7905871868134 | 27.7982711791992 | 37.2802317142487 | 34.8069369792938 | 11.8728759864063 | 178.7905871868134 | 52.798271179199205 | 37.2802317142487 | 109.8069369792938 | |
11.8800760324866 | 28.6995828151703 | 27.795284986496 | 36.5555465221405 | 34.3665361404419 | 11.8800760324866 | 178.6995828151703 | 52.795284986496 | 36.5555465221405 | 109.3665361404419 | |
11.8872760785669 | 28.6165058612824 | 27.7908861637116 | 35.8569085597992 | 33.946567773819 | 11.8872760785669 | 178.6165058612824 | 52.790886163711605 | 35.8569085597992 | 108.946567773819 | |
11.8944761246472 | 28.5409152507782 | 27.7835726737976 | 35.1951777935028 | 33.5517168045044 | 11.8944761246472 | 178.5409152507782 | 52.7835726737976 | 35.1951777935028 | 108.5517168045044 | |
11.9016761707275 | 28.4724354743958 | 27.7729630470276 | 34.5782101154328 | 33.1850469112396 | 11.9016761707275 | 178.4724354743958 | 52.7729630470276 | 34.5782101154328 | 108.1850469112396 | |
11.9088762168078 | 28.4107089042664 | 27.7596294879914 | 34.0108096599579 | 32.848459482193 | 11.9088762168078 | 178.41070890426641 | 52.759629487991404 | 34.0108096599579 | 107.848459482193 | |
11.9160762628881 | 28.3550500869751 | 27.7445912361145 | 33.4950387477875 | 32.5431048870087 | 11.9160762628881 | 178.3550500869751 | 52.7445912361145 | 33.4950387477875 | 107.5431048870087 | |
11.9232763089684 | 28.3042073249817 | 27.728807926178 | 33.0307841300965 | 32.2694540023804 | 11.9232763089684 | 178.3042073249817 | 52.728807926178 | 33.0307841300965 | 107.2694540023804 | |
11.9304763550487 | 28.2566606998444 | 27.7130126953125 | 32.6163530349732 | 32.0272326469422 | 11.9304763550487 | 178.2566606998444 | 52.7130126953125 | 32.6163530349732 | 107.0272326469422 | |
11.937676401129 | 28.2110750675202 | 27.6977777481079 | 32.2490334510803 | 31.8154454231262 | 11.937676401129 | 178.2110750675202 | 52.697777748107896 | 32.2490334510803 | 106.81544542312619 | |
11.9448764472093 | 28.1665861606598 | 27.6835024356842 | 31.9255352020264 | 31.6325187683106 | 11.9448764472093 | 178.1665861606598 | 52.683502435684204 | 31.9255352020264 | 106.6325187683106 | |
11.9520764932896 | 28.1227052211762 | 27.6703357696533 | 31.6423118114472 | 31.4764261245728 | 11.9520764932896 | 178.1227052211762 | 52.6703357696533 | 31.6423118114472 | 106.4764261245728 | |
11.9592765393699 | 28.0791878700257 | 27.658224105835 | 31.3959777355194 | 31.3444793224335 | 11.9592765393699 | 178.0791878700257 | 52.658224105835004 | 31.3959777355194 | 106.3444793224335 | |
11.9664765854502 | 28.035968542099 | 27.6470482349396 | 31.1833798885346 | 31.2332093715668 | 11.9664765854502 | 178.035968542099 | 52.647048234939604 | 31.1833798885346 | 106.2332093715668 | |
11.9736766315305 | 27.9931545257569 | 27.6368319988251 | 31.001365184784 | 31.1384618282318 | 11.9736766315305 | 177.9931545257569 | 52.6368319988251 | 31.001365184784 | 106.1384618282318 | |
11.9808766776108 | 27.951043844223 | 27.6278078556061 | 30.8465659618378 | 31.0559213161469 | 11.9808766776108 | 177.951043844223 | 52.6278078556061 | 30.8465659618378 | 106.05592131614691 | |
11.9880767236911 | 27.9098808765412 | 27.6203393936157 | 30.7154417037964 | 30.9817016124726 | 11.9880767236911 | 177.9098808765412 | 52.6203393936157 | 30.7154417037964 | 105.9817016124726 | |
11.9952767697713 | 27.8698563575745 | 27.6145994663239 | 30.6044518947602 | 30.9126317501068 | 11.9952767697713 | 177.8698563575745 | 52.614599466323895 | 30.6044518947602 | 105.9126317501068 | |
12.0024768158516 | 27.8310775756836 | 27.6103913784027 | 30.5103242397309 | 30.8460652828217 | 12.0024768158516 | 177.8310775756836 | 52.610391378402696 | 30.5103242397309 | 105.8460652828217 | |
12.0096768619319 | 27.7936935424805 | 27.6070713996887 | 30.4299056529999 | 30.7796657085419 | 12.0096768619319 | 177.7936935424805 | 52.6070713996887 | 30.4299056529999 | 105.7796657085419 | |
12.0168769080122 | 27.7578890323639 | 27.6036560535431 | 30.3600728511811 | 30.7116270065308 | 12.0168769080122 | 177.7578890323639 | 52.603656053543105 | 30.3600728511811 | 105.7116270065308 | |
12.0240769540925 | 27.7239263057709 | 27.5992095470429 | 30.2977800369263 | 30.6410312652588 | 12.0240769540925 | 177.7239263057709 | 52.5992095470429 | 30.2977800369263 | 105.6410312652588 | |
12.0312770001728 | 27.6921927928925 | 27.5931417942047 | 30.2404344081879 | 30.5679798126221 | 12.0312770001728 | 177.6921927928925 | 52.5931417942047 | 30.2404344081879 | 105.5679798126221 | |
12.0384770462531 | 27.663266658783 | 27.5853574275971 | 30.186140537262 | 30.4932177066803 | 12.0384770462531 | 177.66326665878302 | 52.5853574275971 | 30.186140537262 | 105.4932177066803 | |
12.0456770923334 | 27.6378154754639 | 27.5761723518372 | 30.1336705684662 | 30.4174423217774 | 12.0456770923334 | 177.6378154754639 | 52.5761723518372 | 30.1336705684662 | 105.4174423217774 | |
12.0528771384137 | 27.6163995265961 | 27.5662660598755 | 30.0821304321289 | 30.3411543369293 | 12.0528771384137 | 177.6163995265961 | 52.5662660598755 | 30.0821304321289 | 105.34115433692929 | |
12.060077184494 | 27.5993168354035 | 27.5565981864929 | 30.0308167934418 | 30.2649974822998 | 12.060077184494 | 177.5993168354035 | 52.5565981864929 | 30.0308167934418 | 105.2649974822998 | |
12.0672772305743 | 27.5865018367768 | 27.5482654571533 | 29.9792349338532 | 30.1901638507843 | 12.0672772305743 | 177.5865018367768 | 52.5482654571533 | 29.9792349338532 | 105.1901638507843 | |
12.0744772766546 | 27.5775849819184 | 27.5422930717468 | 29.9273312091828 | 30.1181614398957 | 12.0744772766546 | 177.5775849819184 | 52.5422930717468 | 29.9273312091828 | 105.1181614398957 | |
12.0816773227349 | 27.571976184845 | 27.5390684604645 | 29.8754990100861 | 30.050253868103 | 12.0816773227349 | 177.571976184845 | 52.5390684604645 | 29.8754990100861 | 105.050253868103 | |
12.0888773688152 | 27.5687575340271 | 27.5380432605744 | 29.8244416713715 | 29.9870133399964 | 12.0888773688152 | 177.5687575340271 | 52.5380432605744 | 29.8244416713715 | 104.9870133399964 | |
12.0960774148955 | 27.5666177272797 | 27.5380074977875 | 29.7749996185303 | 29.9286007881165 | 12.0960774148955 | 177.5666177272797 | 52.538007497787504 | 29.7749996185303 | 104.9286007881165 | |
12.1032774609758 | 27.5638222694397 | 27.5375485420227 | 29.7279536724091 | 29.8753559589386 | 12.1032774609758 | 177.5638222694397 | 52.537548542022705 | 29.7279536724091 | 104.8753559589386 | |
12.1104775070561 | 27.5585949420929 | 27.5356650352478 | 29.6840012073517 | 29.8279821872711 | 12.1104775070561 | 177.5585949420929 | 52.5356650352478 | 29.6840012073517 | 104.8279821872711 | |
12.1176775531364 | 27.5496542453766 | 27.5318682193756 | 29.6436607837677 | 29.7870874404907 | 12.1176775531364 | 177.5496542453766 | 52.531868219375596 | 29.6436607837677 | 104.7870874404907 | |
12.1248775992167 | 27.5366961956024 | 27.5259494781494 | 29.6072721481323 | 29.7527134418488 | 12.1248775992167 | 177.5366961956024 | 52.5259494781494 | 29.6072721481323 | 104.7527134418488 | |
12.132077645297 | 27.5203287601471 | 27.5179207324982 | 29.5749664306641 | 29.7241747379303 | 12.132077645297 | 177.5203287601471 | 52.5179207324982 | 29.5749664306641 | 104.7241747379303 | |
12.1392776913772 | 27.5017976760864 | 27.5081336498261 | 29.5466184616089 | 29.7004818916321 | 12.1392776913772 | 177.5017976760864 | 52.5081336498261 | 29.5466184616089 | 104.70048189163211 | |
12.1464777374575 | 27.4825930595398 | 27.4972796440125 | 29.5218408107758 | 29.6807706356049 | 12.1464777374575 | 177.4825930595398 | 52.4972796440125 | 29.5218408107758 | 104.6807706356049 | |
12.1536777835378 | 27.4643361568451 | 27.4861633777619 | 29.5000791549683 | 29.6643257141113 | 12.1536777835378 | 177.4643361568451 | 52.4861633777619 | 29.5000791549683 | 104.6643257141113 | |
12.1608778296181 | 27.448570728302 | 27.4753153324127 | 29.4807553291321 | 29.6503365039826 | 12.1608778296181 | 177.448570728302 | 52.4753153324127 | 29.4807553291321 | 104.6503365039826 | |
12.1680778756984 | 27.4364292621613 | 27.4647295475006 | 29.4633805751801 | 29.6379208564759 | 12.1680778756984 | 177.4364292621613 | 52.464729547500596 | 29.4633805751801 | 104.6379208564759 | |
12.1752779217787 | 27.4280905723572 | 27.4540901184082 | 29.4476330280304 | 29.6263635158539 | 12.1752779217787 | 177.4280905723572 | 52.4540901184082 | 29.4476330280304 | 104.6263635158539 | |
12.182477967859 | 27.4226546287537 | 27.4431705474854 | 29.4331014156342 | 29.615467786789 | 12.182477967859 | 177.4226546287537 | 52.4431705474854 | 29.4331014156342 | 104.615467786789 | |
12.1896780139393 | 27.4185121059418 | 27.4319887161255 | 29.4193208217621 | 29.6055972576142 | 12.1896780139393 | 177.4185121059418 | 52.4319887161255 | 29.4193208217621 | 104.60559725761419 | |
12.1968780600196 | 27.4139761924744 | 27.4209380149842 | 29.4059038162232 | 29.5972287654877 | 12.1968780600196 | 177.4139761924744 | 52.4209380149842 | 29.4059038162232 | 104.5972287654877 | |
12.2040781060999 | 27.407717704773 | 27.4107277393341 | 29.3927490711212 | 29.5905709266663 | 12.2040781060999 | 177.407717704773 | 52.4107277393341 | 29.3927490711212 | 104.5905709266663 | |
12.2112781521802 | 27.3990690708161 | 27.4023115634918 | 29.3799996376038 | 29.5854210853577 | 12.2112781521802 | 177.3990690708161 | 52.4023115634918 | 29.3799996376038 | 104.5854210853577 | |
12.2184781982605 | 27.3881316184998 | 27.3968040943146 | 29.3679296970368 | 29.5812606811524 | 12.2184781982605 | 177.3881316184998 | 52.396804094314604 | 29.3679296970368 | 104.5812606811524 |