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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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10.7856690282818 | 27.7081489562988 | 28.0233800411225 | 29.7831356525421 | 29.9741089344025 | 10.7856690282818 | 177.7081489562988 | 53.0233800411225 | 29.7831356525421 | 104.9741089344025 | |
10.7928690743621 | 27.6948809623718 | 28.0242621898651 | 29.7594547271729 | 29.9389302730561 | 10.7928690743621 | 177.6948809623718 | 53.0242621898651 | 29.7594547271729 | 104.9389302730561 | |
10.8000691204424 | 27.6816725730896 | 28.0223786830902 | 29.7353208065033 | 29.9000918865204 | 10.8000691204424 | 177.6816725730896 | 53.022378683090196 | 29.7353208065033 | 104.9000918865204 | |
10.8072691665227 | 27.6685953140259 | 28.0190825462342 | 29.7112464904785 | 29.8586428165436 | 10.8072691665227 | 177.6685953140259 | 53.0190825462342 | 29.7112464904785 | 104.85864281654361 | |
10.814469212603 | 27.6554465293884 | 28.0158698558808 | 29.6874940395355 | 29.8156261444092 | 10.814469212603 | 177.6554465293884 | 53.0158698558808 | 29.6874940395355 | 104.81562614440921 | |
10.8216692586833 | 27.64191031456 | 28.0138373374939 | 29.6639978885651 | 29.7722637653351 | 10.8216692586833 | 177.64191031456 | 53.0138373374939 | 29.6639978885651 | 104.7722637653351 | |
10.8288693047636 | 27.6278913021088 | 28.0133903026581 | 29.6404361724854 | 29.7299265861511 | 10.8288693047636 | 177.6278913021088 | 53.013390302658095 | 29.6404361724854 | 104.7299265861511 | |
10.8360693508439 | 27.6135802268982 | 28.0144333839417 | 29.6165049076081 | 29.6897649765015 | 10.8360693508439 | 177.6135802268982 | 53.0144333839417 | 29.6165049076081 | 104.6897649765015 | |
10.8432693969242 | 27.5993585586548 | 28.0166745185852 | 29.5921564102173 | 29.6524703502655 | 10.8432693969242 | 177.59935855865479 | 53.016674518585205 | 29.5921564102173 | 104.6524703502655 | |
10.8504694430045 | 27.5855839252472 | 28.0197203159332 | 29.5677304267884 | 29.6182870864868 | 10.8504694430045 | 177.5855839252472 | 53.0197203159332 | 29.5677304267884 | 104.6182870864868 | |
10.8576694890848 | 27.572649717331 | 28.022974729538 | 29.5438230037689 | 29.5873880386353 | 10.8576694890848 | 177.572649717331 | 53.022974729538 | 29.5438230037689 | 104.5873880386353 | |
10.864869535165 | 27.5611996650696 | 28.0256450176239 | 29.5212924480438 | 29.5600712299347 | 10.864869535165 | 177.5611996650696 | 53.0256450176239 | 29.5212924480438 | 104.56007122993469 | |
10.8720695812453 | 27.5522530078888 | 28.027081489563 | 29.5010566711426 | 29.5364737510681 | 10.8720695812453 | 177.5522530078888 | 53.027081489563 | 29.5010566711426 | 104.5364737510681 | |
10.8792696273256 | 27.5469064712525 | 28.0271470546723 | 29.484099149704 | 29.5163869857788 | 10.8792696273256 | 177.5469064712525 | 53.0271470546723 | 29.484099149704 | 104.51638698577881 | |
10.8864696734059 | 27.5457859039307 | 28.026282787323 | 29.4712364673615 | 29.4993221759796 | 10.8864696734059 | 177.5457859039307 | 53.026282787323 | 29.4712364673615 | 104.4993221759796 | |
10.8936697194862 | 27.5487780570984 | 28.0252039432526 | 29.4627368450165 | 29.4850587844849 | 10.8936697194862 | 177.5487780570984 | 53.0252039432526 | 29.4627368450165 | 104.48505878448489 | |
10.9008697655665 | 27.5552213191986 | 28.024423122406 | 29.4582486152649 | 29.4739365577698 | 10.9008697655665 | 177.5552213191986 | 53.024423122406 | 29.4582486152649 | 104.4739365577698 | |
10.9080698116468 | 27.5641024112702 | 28.0239820480347 | 29.4571161270142 | 29.4664800167084 | 10.9080698116468 | 177.5641024112702 | 53.023982048034696 | 29.4571161270142 | 104.4664800167084 | |
10.9152698577271 | 27.5741934776306 | 28.0235826969147 | 29.4587254524231 | 29.4627249240875 | 10.9152698577271 | 177.57419347763062 | 53.0235826969147 | 29.4587254524231 | 104.4627249240875 | |
10.9224699038074 | 27.5839567184448 | 28.0227184295655 | 29.4627368450165 | 29.4620633125305 | 10.9224699038074 | 177.5839567184448 | 53.0227184295655 | 29.4627368450165 | 104.4620633125305 | |
10.9296699498877 | 27.591609954834 | 28.0207872390747 | 29.4688284397125 | 29.4637084007263 | 10.9296699498877 | 177.59160995483398 | 53.0207872390747 | 29.4688284397125 | 104.4637084007263 | |
10.936869995968 | 27.5955677032471 | 28.0170023441315 | 29.4764161109925 | 29.467248916626 | 10.936869995968 | 177.5955677032471 | 53.0170023441315 | 29.4764161109925 | 104.467248916626 | |
10.9440700420483 | 27.5950312614441 | 28.0105829238892 | 29.4848024845124 | 29.4726848602295 | 10.9440700420483 | 177.5950312614441 | 53.0105829238892 | 29.4848024845124 | 104.47268486022949 | |
10.9512700881286 | 27.5900900363922 | 28.0009210109711 | 29.4936537742615 | 29.4799149036408 | 10.9512700881286 | 177.5900900363922 | 53.0009210109711 | 29.4936537742615 | 104.4799149036408 | |
10.9584701342089 | 27.5816023349762 | 27.9877424240113 | 29.5031368732453 | 29.4884324073792 | 10.9584701342089 | 177.5816023349762 | 52.9877424240113 | 29.5031368732453 | 104.48843240737921 | |
10.9656701802892 | 27.570778131485 | 27.9712438583374 | 29.5137345790863 | 29.4977128505707 | 10.9656701802892 | 177.57077813148499 | 52.9712438583374 | 29.5137345790863 | 104.49771285057071 | |
10.9728702263695 | 27.5589644908905 | 27.9520928859711 | 29.5257449150086 | 29.5077741146088 | 10.9728702263695 | 177.5589644908905 | 52.9520928859711 | 29.5257449150086 | 104.5077741146088 | |
10.9800702724498 | 27.547562122345 | 27.9314339160919 | 29.5389950275421 | 29.5192122459412 | 10.9800702724498 | 177.547562122345 | 52.931433916091905 | 29.5389950275421 | 104.5192122459412 | |
10.9872703185301 | 27.5378346443176 | 27.9107630252838 | 29.5531451702118 | 29.5326411724091 | 10.9872703185301 | 177.5378346443176 | 52.9107630252838 | 29.5531451702118 | 104.5326411724091 | |
10.9944703646104 | 27.5304853916168 | 27.8916358947754 | 29.5680105686188 | 29.5482575893402 | 10.9944703646104 | 177.5304853916168 | 52.891635894775405 | 29.5680105686188 | 104.5482575893402 | |
11.0016704106907 | 27.5254428386688 | 27.8752863407135 | 29.5835375785828 | 29.56582903862 | 11.0016704106907 | 177.5254428386688 | 52.8752863407135 | 29.5835375785828 | 104.56582903862 | |
11.0088704567709 | 27.5219857692719 | 27.8623104095459 | 29.599678516388 | 29.5854210853577 | 11.0088704567709 | 177.5219857692719 | 52.8623104095459 | 29.599678516388 | 104.5854210853577 | |
11.0160705028512 | 27.5191307067871 | 27.852737903595 | 29.6162724494934 | 29.6076774597168 | 11.0160705028512 | 177.5191307067871 | 52.852737903595 | 29.6162724494934 | 104.6076774597168 | |
11.0232705489315 | 27.5161385536194 | 27.8460621833801 | 29.6330213546753 | 29.6336233615875 | 11.0232705489315 | 177.51613855361938 | 52.8460621833801 | 29.6330213546753 | 104.6336233615875 | |
11.0304705950118 | 27.5124788284302 | 27.8414785861969 | 29.6498894691467 | 29.6641051769257 | 11.0304705950118 | 177.5124788284302 | 52.8414785861969 | 29.6498894691467 | 104.6641051769257 | |
11.0376706410921 | 27.5076925754547 | 27.8379797935486 | 29.6672224998474 | 29.6994864940644 | 11.0376706410921 | 177.5076925754547 | 52.8379797935486 | 29.6672224998474 | 104.6994864940644 | |
11.0448706871724 | 27.5014042854309 | 27.8345406055451 | 29.6855211257935 | 29.7395646572113 | 11.0448706871724 | 177.5014042854309 | 52.8345406055451 | 29.6855211257935 | 104.7395646572113 | |
11.0520707332527 | 27.4934709072113 | 27.830457687378 | 29.7051966190338 | 29.7836840152741 | 11.0520707332527 | 177.4934709072113 | 52.830457687378 | 29.7051966190338 | 104.7836840152741 | |
11.059270779333 | 27.484142780304 | 27.8254926204682 | 29.726243019104 | 29.8307657241821 | 11.059270779333 | 177.484142780304 | 52.8254926204682 | 29.726243019104 | 104.8307657241821 | |
11.0664708254133 | 27.473932504654 | 27.819973230362 | 29.748147726059 | 29.8792362213135 | 11.0664708254133 | 177.473932504654 | 52.819973230361995 | 29.748147726059 | 104.8792362213135 | |
11.0736708714936 | 27.4631321430207 | 27.8144836425781 | 29.7701716423035 | 29.9272358417511 | 11.0736708714936 | 177.4631321430207 | 52.8144836425781 | 29.7701716423035 | 104.9272358417511 | |
11.0808709175739 | 27.4516463279724 | 27.809625864029 | 29.7917366027832 | 29.9729585647583 | 11.0808709175739 | 177.4516463279724 | 52.809625864029 | 29.7917366027832 | 104.9729585647583 | |
11.0880709636542 | 27.4393677711487 | 27.8057634830475 | 29.812616109848 | 30.0150990486145 | 11.0880709636542 | 177.4393677711487 | 52.8057634830475 | 29.812616109848 | 105.0150990486145 | |
11.0952710097345 | 27.4266064167023 | 27.8028964996338 | 29.8331320285797 | 30.0531506538391 | 11.0952710097345 | 177.4266064167023 | 52.8028964996338 | 29.8331320285797 | 105.0531506538391 | |
11.1024710558148 | 27.414333820343 | 27.8005957603455 | 29.8541009426117 | 30.0875067710877 | 11.1024710558148 | 177.414333820343 | 52.8005957603455 | 29.8541009426117 | 105.0875067710877 | |
11.1096711018951 | 27.4037837982178 | 27.7980506420136 | 29.8768818378449 | 30.1193475723267 | 11.1096711018951 | 177.4037837982178 | 52.7980506420136 | 29.8768818378449 | 105.1193475723267 | |
11.1168711479754 | 27.3959815502167 | 27.7943253517151 | 29.9034655094147 | 30.1502823829651 | 11.1168711479754 | 177.3959815502167 | 52.7943253517151 | 29.9034655094147 | 105.1502823829651 | |
11.1240711940557 | 27.3916840553284 | 27.7887523174286 | 29.9363553524018 | 30.1821112632752 | 11.1240711940557 | 177.3916840553284 | 52.7887523174286 | 29.9363553524018 | 105.1821112632752 | |
11.131271240136 | 27.3916065692902 | 27.781230211258 | 29.9780488014221 | 30.2165508270264 | 11.131271240136 | 177.3916065692902 | 52.781230211258 | 29.9780488014221 | 105.2165508270264 | |
11.1384712862163 | 27.3965477943421 | 27.7721822261811 | 30.0304055213928 | 30.2551567554474 | 11.1384712862163 | 177.3965477943421 | 52.7721822261811 | 30.0304055213928 | 105.2551567554474 | |
11.1456713322966 | 27.4072289466858 | 27.7622699737549 | 30.0942003726959 | 30.2994310855866 | 11.1456713322966 | 177.4072289466858 | 52.7622699737549 | 30.0942003726959 | 105.2994310855866 | |
11.1528713783768 | 27.4239838123322 | 27.7521550655365 | 30.1691174507141 | 30.3509056568146 | 11.1528713783768 | 177.4239838123322 | 52.7521550655365 | 30.1691174507141 | 105.3509056568146 | |
11.1600714244571 | 27.4467527866364 | 27.742475271225 | 30.2542388439179 | 30.4110527038574 | 11.1600714244571 | 177.4467527866364 | 52.742475271225004 | 30.2542388439179 | 105.4110527038574 | |
11.1672714705374 | 27.4754285812378 | 27.7339339256287 | 30.3483963012696 | 30.4810881614685 | 11.1672714705374 | 177.4754285812378 | 52.733933925628705 | 30.3483963012696 | 105.4810881614685 | |
11.1744715166177 | 27.5101125240326 | 27.7271389961243 | 30.4504990577698 | 30.5616974830628 | 11.1744715166177 | 177.5101125240326 | 52.727138996124296 | 30.4504990577698 | 105.5616974830628 | |
11.181671562698 | 27.5510430335999 | 27.7223229408264 | 30.5597543716431 | 30.6529402732849 | 11.181671562698 | 177.5510430335999 | 52.7223229408264 | 30.5597543716431 | 105.6529402732849 | |
11.1888716087783 | 27.5984108448029 | 27.7191877365113 | 30.6757867336273 | 30.754292011261 | 11.1888716087783 | 177.5984108448029 | 52.7191877365113 | 30.6757867336273 | 105.754292011261 | |
11.1960716548586 | 27.6521146297455 | 27.7170658111572 | 30.798888206482 | 30.864828824997 | 11.1960716548586 | 177.6521146297455 | 52.7170658111572 | 30.798888206482 | 105.864828824997 | |
11.2032717009389 | 27.7118563652039 | 27.7153968811035 | 30.9300065040589 | 30.9833526611328 | 11.2032717009389 | 177.71185636520391 | 52.7153968811035 | 30.9300065040589 | 105.9833526611328 | |
11.2104717470192 | 27.7774214744568 | 27.7140140533447 | 31.07048869133 | 31.1086416244507 | 11.2104717470192 | 177.7774214744568 | 52.7140140533447 | 31.07048869133 | 106.1086416244507 | |
11.2176717930995 | 27.8491258621216 | 27.7130484580994 | 31.2217891216278 | 31.239914894104 | 11.2176717930995 | 177.8491258621216 | 52.7130484580994 | 31.2217891216278 | 106.239914894104 | |
11.2248718391798 | 27.9283702373505 | 27.7126431465149 | 31.3851952552796 | 31.3775837421417 | 11.2248718391798 | 177.9283702373505 | 52.7126431465149 | 31.3851952552796 | 106.3775837421417 | |
11.2320718852601 | 28.018456697464 | 27.7127981185913 | 31.5617620944977 | 31.5241158008576 | 11.2320718852601 | 178.018456697464 | 52.7127981185913 | 31.5617620944977 | 106.5241158008576 | |
11.2392719313404 | 28.1254291534424 | 27.7135372161865 | 31.7525625228882 | 31.6844403743744 | 11.2392719313404 | 178.1254291534424 | 52.713537216186495 | 31.7525625228882 | 106.6844403743744 | |
11.2464719774207 | 28.2588422298432 | 27.7152359485626 | 31.9590389728546 | 31.8660855293274 | 11.2464719774207 | 178.2588422298432 | 52.7152359485626 | 31.9590389728546 | 106.86608552932739 | |
11.253672023501 | 28.4324526786804 | 27.7186930179596 | 32.1832776069641 | 32.0791125297547 | 11.253672023501 | 178.4324526786804 | 52.718693017959595 | 32.1832776069641 | 107.0791125297547 | |
11.2608720695813 | 28.6650061607361 | 27.7248024940491 | 32.4281215667725 | 32.3362410068512 | 11.2608720695813 | 178.6650061607361 | 52.7248024940491 | 32.4281215667725 | 107.3362410068512 | |
11.2680721156616 | 28.9810121059418 | 27.7341306209564 | 32.6972246170044 | 32.653272151947 | 11.2680721156616 | 178.9810121059418 | 52.7341306209564 | 32.6972246170044 | 107.653272151947 | |
11.2752721617419 | 29.4113337993622 | 27.7467012405396 | 32.9949259757996 | 33.049339056015 | 11.2752721617419 | 179.4113337993622 | 52.7467012405396 | 32.9949259757996 | 108.049339056015 | |
11.2824722078222 | 29.9935936927796 | 27.762109041214 | 33.3263218402863 | 33.547180891037 | 11.2824722078222 | 179.9935936927796 | 52.762109041214 | 33.3263218402863 | 108.547180891037 | |
11.2896722539024 | 30.7722389698029 | 27.779895067215 | 33.6973488330841 | 34.1732919216156 | 11.2896722539024 | 180.7722389698029 | 52.779895067215 | 33.6973488330841 | 109.1732919216156 | |
11.2968722999827 | 31.7987322807312 | 27.7995765209198 | 34.1148734092713 | 34.9584877490998 | 11.2968722999827 | 181.7987322807312 | 52.7995765209198 | 34.1148734092713 | 109.9584877490998 | |
11.304072346063 | 33.1317126750946 | 27.8205156326294 | 34.5867812633515 | 35.9385192394257 | 11.304072346063 | 183.1317126750946 | 52.8205156326294 | 34.5867812633515 | 110.9385192394257 | |
11.3112723921433 | 34.8365962505341 | 27.8419613838196 | 35.122299194336 | 37.1543347835541 | 11.3112723921433 | 184.83659625053411 | 52.8419613838196 | 35.122299194336 | 112.1543347835541 | |
11.3184724382236 | 36.983996629715 | 27.8633773326874 | 35.7324421405792 | 38.6514604091644 | 11.3184724382236 | 186.983996629715 | 52.8633773326874 | 35.7324421405792 | 113.6514604091644 | |
11.3256724843039 | 39.6465837955475 | 27.8850734233856 | 36.4303469657898 | 40.4785215854645 | 11.3256724843039 | 189.64658379554749 | 52.8850734233856 | 36.4303469657898 | 115.4785215854645 | |
11.3328725303842 | 42.8950548171997 | 27.908605337143 | 37.2315526008606 | 42.6849126815796 | 11.3328725303842 | 192.8950548171997 | 52.908605337143 | 37.2315526008606 | 117.68491268157959 | |
11.3400725764645 | 46.7937290668488 | 27.9367029666901 | 38.153737783432 | 45.3181207180023 | 11.3400725764645 | 196.7937290668488 | 52.9367029666901 | 38.153737783432 | 120.31812071800229 | |
11.3472726225448 | 51.3960242271424 | 27.9728472232819 | 39.2160356044769 | 48.4203457832337 | 11.3472726225448 | 201.3960242271424 | 52.9728472232819 | 39.2160356044769 | 123.4203457832337 | |
11.3544726686251 | 56.7393660545349 | 28.0209004878998 | 40.4380559921265 | 52.0245313644409 | 11.3544726686251 | 206.7393660545349 | 53.0209004878998 | 40.4380559921265 | 127.02453136444089 | |
11.3616727147054 | 62.8393769264221 | 28.0847370624542 | 41.8381631374359 | 56.1503231525421 | 11.3616727147054 | 212.8393769264221 | 53.084737062454195 | 41.8381631374359 | 131.15032315254211 | |
11.3688727607857 | 69.6843624114991 | 28.1679213047028 | 43.4314608573914 | 60.8001947402954 | 11.3688727607857 | 219.68436241149908 | 53.1679213047028 | 43.4314608573914 | 135.8001947402954 | |
11.376072806866 | 77.2315084934235 | 28.2733738422394 | 45.2277064323426 | 65.9561097621918 | 11.376072806866 | 227.23150849342352 | 53.2733738422394 | 45.2277064323426 | 140.9561097621918 | |
11.3832728529463 | 85.4055404663086 | 28.4030199050904 | 47.2294747829437 | 71.5772032737732 | 11.3832728529463 | 235.4055404663086 | 53.4030199050904 | 47.2294747829437 | 146.5772032737732 | |
11.3904728990266 | 94.0990567207337 | 28.5577595233917 | 49.4307100772858 | 77.5982797145844 | 11.3904728990266 | 244.0990567207337 | 53.557759523391695 | 49.4307100772858 | 152.5982797145844 | |
11.3976729451069 | 103.173810243607 | 28.7375926971436 | 51.815664768219 | 83.9297235012055 | 11.3976729451069 | 253.173810243607 | 53.7375926971436 | 51.815664768219 | 158.9297235012055 | |
11.4048729911872 | 112.463200092316 | 28.9419531822205 | 54.3579578399659 | 90.4589474201203 | 11.4048729911872 | 262.463200092316 | 53.9419531822205 | 54.3579578399659 | 165.4589474201203 | |
11.4120730372675 | 121.776551008225 | 29.1701555252075 | 57.0203244686127 | 97.0533072948456 | 11.4120730372675 | 271.776551008225 | 54.170155525207505 | 57.0203244686127 | 172.05330729484558 | |
11.4192730833478 | 130.906349420548 | 29.4217705726624 | 59.75541472435 | 103.564602136612 | 11.4192730833478 | 280.906349420548 | 54.421770572662396 | 59.75541472435 | 178.564602136612 | |
11.4264731294281 | 139.637440443039 | 29.6966373920441 | 62.5074923038483 | 109.835314750672 | 11.4264731294281 | 289.637440443039 | 54.696637392044096 | 62.5074923038483 | 184.835314750672 | |
11.4336731755083 | 147.756630182266 | 29.9946129322052 | 65.2149200439453 | 115.705865621567 | 11.4336731755083 | 297.756630182266 | 54.9946129322052 | 65.2149200439453 | 190.705865621567 | |
11.4408732215886 | 155.061638355255 | 30.3152680397034 | 67.8129971027375 | 121.022421121598 | 11.4408732215886 | 305.061638355255 | 55.3152680397034 | 67.8129971027375 | 196.022421121598 | |
11.4480732676689 | 161.369508504868 | 30.6575953960419 | 70.2366352081299 | 125.644052028656 | 11.4480732676689 | 311.369508504868 | 55.6575953960419 | 70.2366352081299 | 200.644052028656 | |
11.4552733137492 | 166.524404287338 | 31.0200095176697 | 72.4232017993927 | 129.448920488358 | 11.4552733137492 | 316.52440428733803 | 56.0200095176697 | 72.4232017993927 | 204.448920488358 | |
11.4624733598295 | 170.404535531998 | 31.3999533653259 | 74.3154168128968 | 132.339900732041 | 11.4624733598295 | 320.404535531998 | 56.3999533653259 | 74.3154168128968 | 207.339900732041 | |
11.4696734059098 | 172.92709350586 | 31.793338060379 | 75.8638918399811 | 134.24905538559 | 11.4696734059098 | 322.92709350586 | 56.793338060379 | 75.8638918399811 | 209.24905538559 | |
11.4768734519901 | 174.050003290177 | 32.1941912174225 | 77.0293354988098 | 135.140335559845 | 11.4768734519901 | 324.05000329017696 | 57.1941912174225 | 77.0293354988098 | 210.140335559845 | |
11.4840734980704 | 173.771303892136 | 32.5949788093567 | 77.7839839458466 | 135.00988483429 | 11.4840734980704 | 323.77130389213596 | 57.5949788093567 | 77.7839839458466 | 210.00988483429 | |
11.4912735441507 | 172.127383947373 | 32.9875230789185 | 78.112143278122 | 133.884024620056 | 11.4912735441507 | 322.127383947373 | 57.9875230789185 | 78.112143278122 | 208.884024620056 | |
11.498473590231 | 169.190901517868 | 33.3637535572052 | 78.0102550983429 | 131.81643486023 | 11.498473590231 | 319.19090151786804 | 58.3637535572052 | 78.0102550983429 | 206.81643486023 |