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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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10.0656644202523 | 174.410420656204 | 34.6377313137055 | 183.487874269486 | 147.799515724182 | 10.0656644202523 | 324.410420656204 | 59.6377313137055 | 183.487874269486 | 222.799515724182 | |
10.0728644663326 | 181.531608104706 | 35.1864933967591 | 193.67191195488 | 154.663723707199 | 10.0728644663326 | 331.53160810470604 | 60.1864933967591 | 193.67191195488 | 229.663723707199 | |
10.0800645124129 | 186.889463663101 | 35.7070505619049 | 202.197885513306 | 160.177725553513 | 10.0800645124129 | 336.88946366310097 | 60.7070505619049 | 202.197885513306 | 235.177725553513 | |
10.0872645584932 | 190.299236774445 | 36.1828744411469 | 208.775585889816 | 164.160585403443 | 10.0872645584932 | 340.29923677444503 | 61.1828744411469 | 208.775585889816 | 239.160585403443 | |
10.0944646045735 | 191.648805141449 | 36.5980565547943 | 213.184016942978 | 166.488295793534 | 10.0944646045735 | 341.648805141449 | 61.5980565547943 | 213.184016942978 | 241.488295793534 | |
10.1016646506538 | 190.906167030335 | 36.938488483429 | 215.284776687622 | 167.099857330322 | 10.1016646506538 | 340.90616703033504 | 61.938488483429 | 215.284776687622 | 242.099857330322 | |
10.1088646967341 | 188.122111558914 | 37.1924877166748 | 215.030491352082 | 165.999430418015 | 10.1088646967341 | 338.122111558914 | 62.1924877166748 | 215.030491352082 | 240.999430418015 | |
10.1160647428144 | 183.426368236542 | 37.3509466648102 | 212.466013431549 | 163.254797458649 | 10.1160647428144 | 333.426368236542 | 62.3509466648102 | 212.466013431549 | 238.254797458649 | |
10.1232647888947 | 177.017742395401 | 37.4075293540955 | 207.722806930542 | 158.992272615433 | 10.1232647888947 | 327.017742395401 | 62.4075293540955 | 207.722806930542 | 233.992272615433 | |
10.130464834975 | 169.150221347809 | 37.3593032360077 | 201.008677482605 | 153.388941287995 | 10.130464834975 | 319.150221347809 | 62.3593032360077 | 201.008677482605 | 228.388941287995 | |
10.1376648810553 | 160.117161273957 | 37.2074723243714 | 192.594385147095 | 146.662211418152 | 10.1376648810553 | 310.117161273957 | 62.2074723243714 | 192.594385147095 | 221.662211418152 | |
10.1448649271356 | 150.234496593476 | 36.9576513767243 | 182.797980308533 | 139.056885242462 | 10.1448649271356 | 300.234496593476 | 61.9576513767243 | 182.797980308533 | 214.056885242462 | |
10.1520649732158 | 139.823001623154 | 36.619246006012 | 171.966457366944 | 130.830949544907 | 10.1520649732158 | 289.823001623154 | 61.619246006012 | 171.966457366944 | 205.830949544907 | |
10.1592650192961 | 129.190826416016 | 36.2045466899872 | 160.455358028412 | 122.241324186325 | 10.1592650192961 | 279.19082641601597 | 61.2045466899872 | 160.455358028412 | 197.24132418632502 | |
10.1664650653764 | 118.618839979172 | 35.7277691364289 | 148.609435558319 | 113.530898094177 | 10.1664650653764 | 268.618839979172 | 60.7277691364289 | 148.609435558319 | 188.53089809417702 | |
10.1736651114567 | 108.350223302841 | 35.2045476436615 | 136.747080087662 | 104.918158054352 | 10.1736651114567 | 258.350223302841 | 60.2045476436615 | 136.747080087662 | 179.918158054352 | |
10.180865157537 | 98.584133386612 | 34.6512973308563 | 125.148874521256 | 96.5895712375641 | 10.180865157537 | 248.584133386612 | 59.6512973308563 | 125.148874521256 | 171.5895712375641 | |
10.1880652036173 | 89.4727647304535 | 34.0842843055725 | 114.049857854843 | 88.6945724487305 | 10.1880652036173 | 239.4727647304535 | 59.0842843055725 | 114.049857854843 | 163.6945724487305 | |
10.1952652496976 | 81.1212182044983 | 33.5183680057526 | 103.634077310562 | 81.3435196876526 | 10.1952652496976 | 231.1212182044983 | 58.5183680057526 | 103.634077310562 | 156.3435196876526 | |
10.2024652957779 | 73.5899746418 | 32.9660534858704 | 94.0321445465088 | 74.6086776256562 | 10.2024652957779 | 223.58997464179998 | 57.9660534858704 | 94.0321445465088 | 149.60867762565618 | |
10.2096653418582 | 66.8999195098877 | 32.4369251728058 | 85.3227734565735 | 68.5276746749878 | 10.2096653418582 | 216.8999195098877 | 57.4369251728058 | 85.3227734565735 | 143.5276746749878 | |
10.2168653879385 | 61.0389649868012 | 31.93798661232 | 77.5382578372956 | 63.1089687347412 | 10.2168653879385 | 211.0389649868012 | 56.93798661232 | 77.5382578372956 | 138.1089687347412 | |
10.2240654340188 | 55.9692263603211 | 31.4741611480713 | 70.672208070755 | 58.3378970623017 | 10.2240654340188 | 205.9692263603211 | 56.4741611480713 | 70.672208070755 | 133.3378970623017 | |
10.2312654800991 | 51.6339898109436 | 31.0486733913422 | 64.6879076957703 | 54.1820645332337 | 10.2312654800991 | 201.6339898109436 | 56.0486733913422 | 64.6879076957703 | 129.1820645332337 | |
10.2384655261794 | 47.9643285274506 | 30.6632459163666 | 59.5262348651886 | 50.5961418151856 | 10.2384655261794 | 197.9643285274506 | 55.6632459163666 | 59.5262348651886 | 125.5961418151856 | |
10.2456655722597 | 44.8849022388458 | 30.3181648254395 | 55.1133453845978 | 47.5264191627503 | 10.2456655722597 | 194.8849022388458 | 55.318164825439496 | 55.1133453845978 | 122.5264191627503 | |
10.25286561834 | 42.3188865184784 | 30.0125539302826 | 51.3677000999451 | 44.9151813983917 | 10.25286561834 | 192.3188865184784 | 55.0125539302826 | 51.3677000999451 | 119.9151813983917 | |
10.2600656644203 | 40.1916921138764 | 29.7449171543122 | 48.2060849666596 | 42.7047789096832 | 10.2600656644203 | 190.1916921138764 | 54.744917154312205 | 48.2060849666596 | 117.7047789096832 | |
10.2672657105006 | 38.4336292743683 | 29.513543844223 | 45.5479860305786 | 40.8405065536499 | 10.2672657105006 | 188.4336292743683 | 54.513543844223 | 45.5479860305786 | 115.8405065536499 | |
10.2744657565809 | 36.9817852973938 | 29.3166100978852 | 43.3185636997223 | 39.2720878124237 | 10.2744657565809 | 186.9817852973938 | 54.3166100978852 | 43.3185636997223 | 114.2720878124237 | |
10.2816658026612 | 35.7810854911804 | 29.1517555713654 | 41.4503991603852 | 37.9541754722595 | 10.2816658026612 | 185.7810854911804 | 54.1517555713654 | 41.4503991603852 | 112.9541754722595 | |
10.2888658487415 | 34.7846508026123 | 29.0159404277802 | 39.8844420909882 | 36.8465900421143 | 10.2888658487415 | 184.7846508026123 | 54.0159404277802 | 39.8844420909882 | 111.8465900421143 | |
10.2960658948217 | 33.9535117149353 | 28.9054393768311 | 38.5700702667237 | 35.9144508838654 | 10.2960658948217 | 183.9535117149353 | 53.9054393768311 | 38.5700702667237 | 110.9144508838654 | |
10.303265940902 | 33.2559108734131 | 28.8162887096405 | 37.4644279479981 | 35.1281106472016 | 10.303265940902 | 183.2559108734131 | 53.8162887096405 | 37.4644279479981 | 110.1281106472016 | |
10.3104659869823 | 32.6663553714752 | 28.7445902824402 | 36.5315675735474 | 34.4627678394318 | 10.3104659869823 | 182.6663553714752 | 53.7445902824402 | 36.5315675735474 | 109.46276783943179 | |
10.3176660330626 | 32.1645975112915 | 28.6867380142212 | 35.7415318489075 | 33.8978171348572 | 10.3176660330626 | 182.1645975112915 | 53.6867380142212 | 35.7415318489075 | 108.8978171348572 | |
10.3248660791429 | 31.734573841095 | 28.6393105983734 | 35.0694537162781 | 33.4161818027497 | 10.3248660791429 | 181.734573841095 | 53.6393105983734 | 35.0694537162781 | 108.41618180274969 | |
10.3320661252232 | 31.363183259964 | 28.5991907119751 | 34.4948530197144 | 33.0038487911225 | 10.3320661252232 | 181.363183259964 | 53.5991907119751 | 34.4948530197144 | 108.0038487911225 | |
10.3392661713035 | 31.0393750667572 | 28.5637795925141 | 34.0009212493897 | 32.6495528221131 | 10.3392661713035 | 181.0393750667572 | 53.563779592514095 | 34.0009212493897 | 107.6495528221131 | |
10.3464662173838 | 30.7536542415619 | 28.5313367843628 | 33.573853969574 | 32.3444783687592 | 10.3464662173838 | 180.7536542415619 | 53.5313367843628 | 33.573853969574 | 107.3444783687592 | |
10.3536662634641 | 30.4980933666229 | 28.5009920597077 | 33.2025408744812 | 32.0819139480591 | 10.3536662634641 | 180.4980933666229 | 53.5009920597077 | 33.2025408744812 | 107.08191394805911 | |
10.3608663095444 | 30.2664875984192 | 28.4726023674011 | 32.8782916069031 | 31.8567931652069 | 10.3608663095444 | 180.2664875984192 | 53.4726023674011 | 32.8782916069031 | 106.8567931652069 | |
10.3680663556247 | 30.0542712211609 | 28.4463584423065 | 32.5944483280182 | 31.6651105880738 | 10.3680663556247 | 180.0542712211609 | 53.446358442306504 | 32.5944483280182 | 106.6651105880738 | |
10.375266401705 | 29.8582375049591 | 28.422474861145 | 32.345986366272 | 31.5033316612244 | 10.375266401705 | 179.8582375049591 | 53.422474861145005 | 32.345986366272 | 106.5033316612244 | |
10.3824664477853 | 29.6763360500336 | 28.400981426239 | 32.1290373802185 | 31.3680171966553 | 10.3824664477853 | 179.6763360500336 | 53.400981426239 | 32.1290373802185 | 106.3680171966553 | |
10.3896664938656 | 29.5076608657837 | 28.3817529678345 | 31.9403946399689 | 31.2557816505432 | 10.3896664938656 | 179.5076608657837 | 53.3817529678345 | 31.9403946399689 | 106.2557816505432 | |
10.3968665399459 | 29.3521344661713 | 28.3644914627075 | 31.7772746086121 | 31.1633467674256 | 10.3968665399459 | 179.3521344661713 | 53.364491462707505 | 31.7772746086121 | 106.1633467674256 | |
10.4040665860262 | 29.2101204395294 | 28.3487617969513 | 31.637054681778 | 31.0876607894898 | 10.4040665860262 | 179.2101204395294 | 53.3487617969513 | 31.637054681778 | 106.0876607894898 | |
10.4112666321065 | 29.0818452835083 | 28.3339500427246 | 31.5171003341675 | 31.0258328914643 | 10.4112666321065 | 179.0818452835083 | 53.333950042724595 | 31.5171003341675 | 106.0258328914643 | |
10.4184666781868 | 28.9670109748841 | 28.3191919326782 | 31.4147293567658 | 30.9750735759735 | 10.4184666781868 | 178.9670109748841 | 53.3191919326782 | 31.4147293567658 | 105.9750735759735 | |
10.4256667242671 | 28.8650035858155 | 28.3035755157471 | 31.3271701335907 | 30.9325933456421 | 10.4256667242671 | 178.8650035858155 | 53.3035755157471 | 31.3271701335907 | 105.9325933456421 | |
10.4328667703474 | 28.7751197814942 | 28.2864809036255 | 31.2516272068024 | 30.8957576751709 | 10.4328667703474 | 178.7751197814942 | 53.2864809036255 | 31.2516272068024 | 105.8957576751709 | |
10.4400668164276 | 28.6966323852539 | 28.2680094242096 | 31.1852812767029 | 30.8622717857361 | 10.4400668164276 | 178.6966323852539 | 53.268009424209595 | 31.1852812767029 | 105.8622717857361 | |
10.4472668625079 | 28.6286950111389 | 28.2489836215973 | 31.1253428459168 | 30.8302879333496 | 10.4472668625079 | 178.6286950111389 | 53.248983621597304 | 31.1253428459168 | 105.8302879333496 | |
10.4544669085882 | 28.5702466964722 | 28.2306849956513 | 31.069153547287 | 30.7983219623566 | 10.4544669085882 | 178.5702466964722 | 53.2306849956513 | 31.069153547287 | 105.7983219623566 | |
10.4616669546685 | 28.5200238227844 | 28.2143950462342 | 31.0145139694214 | 30.765050649643 | 10.4616669546685 | 178.5200238227844 | 53.2143950462342 | 31.0145139694214 | 105.765050649643 | |
10.4688670007488 | 28.4768700599671 | 28.2013177871704 | 30.9599936008454 | 30.7292997837067 | 10.4688670007488 | 178.4768700599671 | 53.201317787170396 | 30.9599936008454 | 105.7292997837067 | |
10.4760670468291 | 28.439873456955 | 28.1925261020661 | 30.9049427509308 | 30.6903660297394 | 10.4760670468291 | 178.439873456955 | 53.1925261020661 | 30.9049427509308 | 105.69036602973941 | |
10.4832670929094 | 28.4083247184754 | 28.188705444336 | 30.8493196964264 | 30.6482911109925 | 10.4832670929094 | 178.4083247184754 | 53.188705444336 | 30.8493196964264 | 105.6482911109925 | |
10.4904671389897 | 28.3816277980805 | 28.1897068023682 | 30.7935893535614 | 30.6039392948151 | 10.4904671389897 | 178.3816277980805 | 53.1897068023682 | 30.7935893535614 | 105.60393929481509 | |
10.49766718507 | 28.3592343330384 | 28.194147348404 | 30.7384908199311 | 30.5586576461792 | 10.49766718507 | 178.3592343330384 | 53.194147348404 | 30.7384908199311 | 105.5586576461792 | |
10.5048672311503 | 28.3406734466553 | 28.1997382640839 | 30.6849181652069 | 30.5138826370239 | 10.5048672311503 | 178.3406734466553 | 53.199738264083905 | 30.6849181652069 | 105.5138826370239 | |
10.5120672772306 | 28.3256351947785 | 28.2041251659394 | 30.6335270404816 | 30.4708898067475 | 10.5120672772306 | 178.3256351947785 | 53.2041251659394 | 30.6335270404816 | 105.4708898067475 | |
10.5192673233109 | 28.3138751983643 | 28.2054960727692 | 30.5845081806183 | 30.4308950901032 | 10.5192673233109 | 178.31387519836431 | 53.2054960727692 | 30.5845081806183 | 105.4308950901032 | |
10.5264673693912 | 28.3050358295441 | 28.2027244567871 | 30.5376470088959 | 30.3951442241669 | 10.5264673693912 | 178.3050358295441 | 53.202724456787095 | 30.5376470088959 | 105.3951442241669 | |
10.5336674154715 | 28.2985150814057 | 28.1952559947968 | 30.4927289485932 | 30.3647339344025 | 10.5336674154715 | 178.2985150814057 | 53.195255994796796 | 30.4927289485932 | 105.3647339344025 | |
10.5408674615518 | 28.2934665679932 | 28.1831920146942 | 30.4499208927155 | 30.3402781486511 | 10.5408674615518 | 178.2934665679932 | 53.1831920146942 | 30.4499208927155 | 105.3402781486511 | |
10.5480675076321 | 28.2888174057007 | 28.1675696372986 | 30.4098069667816 | 30.3217351436615 | 10.5480675076321 | 178.2888174057007 | 53.1675696372986 | 30.4098069667816 | 105.3217351436615 | |
10.5552675537124 | 28.2834470272064 | 28.1503677368164 | 30.3731799125672 | 30.308610200882 | 10.5552675537124 | 178.2834470272064 | 53.1503677368164 | 30.3731799125672 | 105.308610200882 | |
10.5624675997927 | 28.2763659954071 | 28.133898973465 | 30.3408265113831 | 30.3003489971161 | 10.5624675997927 | 178.2763659954071 | 53.133898973465 | 30.3408265113831 | 105.3003489971161 | |
10.569667645873 | 28.2669007778168 | 28.1200349330902 | 30.3134083747864 | 30.2966117858887 | 10.569667645873 | 178.2669007778168 | 53.120034933090196 | 30.3134083747864 | 105.2966117858887 | |
10.5768676919533 | 28.2548129558563 | 28.1098425388336 | 30.2911579608917 | 30.2970707416535 | 10.5768676919533 | 178.2548129558563 | 53.109842538833604 | 30.2911579608917 | 105.2970707416535 | |
10.5840677380335 | 28.2403230667114 | 28.1037330627442 | 30.2734613418579 | 30.300909280777 | 10.5840677380335 | 178.2403230667114 | 53.1037330627442 | 30.2734613418579 | 105.300909280777 | |
10.5912677841138 | 28.2238245010376 | 28.1017065048218 | 30.2586555480957 | 30.3066313266754 | 10.5912677841138 | 178.2238245010376 | 53.1017065048218 | 30.2586555480957 | 105.3066313266754 | |
10.5984678301941 | 28.2058238983155 | 28.1033277511597 | 30.2442371845246 | 30.3122997283936 | 10.5984678301941 | 178.2058238983155 | 53.103327751159696 | 30.2442371845246 | 105.3122997283936 | |
10.6056678762744 | 28.1868636608124 | 28.107613325119 | 30.2275776863098 | 30.3161084651947 | 10.6056678762744 | 178.1868636608124 | 53.107613325119004 | 30.2275776863098 | 105.3161084651947 | |
10.6128679223547 | 28.1674861907959 | 28.1132161617279 | 30.2067458629608 | 30.316698551178 | 10.6128679223547 | 178.1674861907959 | 53.113216161727905 | 30.2067458629608 | 105.316698551178 | |
10.620067968435 | 28.1479895114899 | 28.1189024448395 | 30.1809668540955 | 30.3129851818085 | 10.620067968435 | 178.1479895114899 | 53.1189024448395 | 30.1809668540955 | 105.3129851818085 | |
10.6272680145153 | 28.1282484531403 | 28.1240940093994 | 30.1506459712982 | 30.3039610385895 | 10.6272680145153 | 178.12824845314032 | 53.1240940093994 | 30.1506459712982 | 105.3039610385895 | |
10.6344680605956 | 28.1076431274414 | 28.1287968158722 | 30.1172971725464 | 30.2887976169586 | 10.6344680605956 | 178.1076431274414 | 53.1287968158722 | 30.1172971725464 | 105.2887976169586 | |
10.6416681066759 | 28.0852854251862 | 28.133100271225 | 30.0832450389862 | 30.2673995494843 | 10.6416681066759 | 178.0852854251862 | 53.133100271225004 | 30.0832450389862 | 105.2673995494843 | |
10.6488681527562 | 28.0604839324951 | 28.1367421150208 | 30.0512552261353 | 30.2408039569855 | 10.6488681527562 | 178.0604839324951 | 53.1367421150208 | 30.0512552261353 | 105.2408039569855 | |
10.6560681988365 | 28.0330061912537 | 28.1390488147736 | 30.0238907337189 | 30.2109777927399 | 10.6560681988365 | 178.0330061912537 | 53.1390488147736 | 30.0238907337189 | 105.2109777927399 | |
10.6632682449168 | 28.0030906200409 | 28.1392991542816 | 30.0026297569275 | 30.1801919937134 | 10.6632682449168 | 178.0030906200409 | 53.1392991542816 | 30.0026297569275 | 105.18019199371341 | |
10.6704682909971 | 27.9713988304138 | 28.1368732452393 | 29.9874603748322 | 30.1503539085388 | 10.6704682909971 | 177.9713988304138 | 53.1368732452393 | 29.9874603748322 | 105.1503539085388 | |
10.6776683370774 | 27.9389142990113 | 28.1311631202698 | 29.9770712852478 | 30.1230072975159 | 10.6776683370774 | 177.9389142990113 | 53.131163120269804 | 29.9770712852478 | 105.1230072975159 | |
10.6848683831577 | 27.906858921051 | 28.1214237213135 | 29.9695312976837 | 30.0994873046875 | 10.6848683831577 | 177.906858921051 | 53.121423721313505 | 29.9695312976837 | 105.0994873046875 | |
10.692068429238 | 27.8766930103302 | 28.1070590019226 | 29.9629807472229 | 30.0810039043427 | 10.692068429238 | 177.8766930103302 | 53.1070590019226 | 29.9629807472229 | 105.08100390434271 | |
10.6992684753183 | 27.8498411178589 | 28.0882120132446 | 29.9560308456421 | 30.0683379173279 | 10.6992684753183 | 177.8498411178589 | 53.0882120132446 | 29.9560308456421 | 105.0683379173279 | |
10.7064685213986 | 27.8273046016693 | 28.0661523342133 | 29.9477934837342 | 30.0614297389984 | 10.7064685213986 | 177.8273046016693 | 53.0661523342133 | 29.9477934837342 | 105.0614297389984 | |
10.7136685674789 | 27.8094470500946 | 28.0431747436524 | 29.9379169940949 | 30.0593972206116 | 10.7136685674789 | 177.8094470500946 | 53.0431747436524 | 29.9379169940949 | 105.0593972206116 | |
10.7208686135592 | 27.795821428299 | 28.0219614505768 | 29.9265801906586 | 30.0608336925507 | 10.7208686135592 | 177.795821428299 | 53.021961450576796 | 29.9265801906586 | 105.0608336925507 | |
10.7280686596394 | 27.785462141037 | 28.0048608779907 | 29.9143850803375 | 30.0641179084778 | 10.7280686596394 | 177.785462141037 | 53.0048608779907 | 29.9143850803375 | 105.0641179084778 | |
10.7352687057197 | 27.7771532535553 | 27.9935121536255 | 29.9018919467926 | 30.0674855709076 | 10.7352687057197 | 177.7771532535553 | 52.9935121536255 | 29.9018919467926 | 105.06748557090759 | |
10.7424687518 | 27.7697443962097 | 27.9885590076447 | 29.8892676830292 | 30.0691246986389 | 10.7424687518 | 177.7697443962097 | 52.988559007644696 | 29.8892676830292 | 105.0691246986389 | |
10.7496687978803 | 27.7622640132904 | 27.9895484447479 | 29.8760950565338 | 30.0672829151154 | 10.7496687978803 | 177.7622640132904 | 52.989548444747896 | 29.8760950565338 | 105.0672829151154 | |
10.7568688439606 | 27.7539491653443 | 27.9951453208924 | 29.8616349697113 | 30.0605654716492 | 10.7568688439606 | 177.7539491653443 | 52.995145320892405 | 29.8616349697113 | 105.0605654716492 | |
10.7640688900409 | 27.7443408966065 | 28.0033648014069 | 29.8452198505402 | 30.0480663776398 | 10.7640688900409 | 177.7443408966065 | 53.0033648014069 | 29.8452198505402 | 105.0480663776398 | |
10.7712689361212 | 27.7333378791809 | 28.0120193958283 | 29.8265337944031 | 30.0293862819672 | 10.7712689361212 | 177.7333378791809 | 53.012019395828304 | 29.8265337944031 | 105.02938628196719 | |
10.7784689822015 | 27.7211248874665 | 28.0191421508789 | 29.8056900501251 | 30.0045609474182 | 10.7784689822015 | 177.7211248874665 | 53.0191421508789 | 29.8056900501251 | 105.0045609474182 |