Testing thiamine metabolites in Col and prr7prr9

Selected Cell

Cell:

Value:

Read.Me Sheet1 Results-std curve Repeat for thiamin Graph-stats Graph-stats (2) Chromatogram

Testing thiamine vitamers in plant samples from study L&H3
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites

Analysis Protocol	From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight.

Sheets of this workbook	Contents
Sheet1	Raw data and sampling plan
Results-std curve	Data for individual replicates and standard curve
Repeat for thiamin
Graph-stats	Summary statistics, means, t-test statistics except thiamine, and final charts
Graph-stats (2)	Summary statistics, means, t-test statistics only for thiamine
Chromatogram	raw data traces

Credit
Experimental design, plant growth and sampling	Yin Hoon Chew, Andrew Millar group University of Edinburgh
Analytical design, execution and data analysis	Michael Moulin, Teresa Fitzpatrick group University of Geneva
File Curation	Andrew Millar




numbering	Col				prr7,prr9					replicat	Col				prr7,prr9
27 EN	1	2	3	4	5	6	7	8		27 EN	1	2	3	4	1	2	3	4
28 ED	9	10	11	12	13	14	15	16		28 ED	1	2	3	4	1	2	3	4
28 EN	17	18	19	20	21	22	23	24		28 EN	1	2	3	4	1	2	3	4

mass tube	Col				prr7,prr9
27 EN	0.9438	0.9547	0.9455	0.9373	0.9437	0.9561	0.9486	0.9465
28 ED	0.9427	0.9391	0.9409	0.9434	0.9522	0.9437	0.9438	0.9442
28 EN	0.944	0.9421	0.9439	0.9465	0.9562	0.9436	0.9506	0.9442

mass tube+sample	Col				prr7,prr9
27 EN	1.0289	1.0105	0.992	0.9987	1.0115	1.0188	1.0039	1.0221
28 ED	1.0234	1.036	1.0222	1.0111	1.0581	0.9944	0.9984	1.0461
28 EN	1.0562	1.0187	1.0434	1.0493	1.0576	1.0668	1.0598	1.0178

mass sample	Col				prr7,prr9
27 EN	0.08509999999999995	0.05579999999999996	0.046499999999999986	0.06140000000000001	0.06780000000000008	0.06269999999999998	0.055300000000000016	0.0756
28 ED	0.0807000000000001	0.09689999999999999	0.08130000000000004	0.0677000000000001	0.1059	0.05069999999999997	0.05459999999999998	0.10189999999999999
28 EN	0.11220000000000008	0.07659999999999989	0.09950000000000014	0.10279999999999989	0.10140000000000005	0.12319999999999998	0.10920000000000007	0.0736

mass sample	Col				prr7,prr9
27 EN	300	200	200	200	200	200	200	300
28 ED	300	300	300	200	400	200	200	400
28 EN	300	300	300	300	40	400	400	300


	Extraxtion volume	Mass			Sample analysis order		TDP	TMP	THIA	Extraction volume	Mass
1	300	85.09999999999995			0.03125	pmol	98.93505859375	100.81101989746094	217.923583984375
2	200	55.79999999999996			0.0625	pmol	252.35	237.66	289.42
3	200	46.499999999999986			0.125	pmol	309.66	318.61	576.96
4	200	61.40000000000001			0.25	pmol	502.94	515.39	955.81
5	200	67.80000000000008			0.5	pmol	850.95	859.87	1269.7
6	200	62.699999999999974			1	pmol	1621.6	1673.7	2243.1
7	200	55.30000000000002			2	pmol	3490.9	3645	4747
8	300	75.6			4	pmol	6814.1	7077	9161
9	300	80.7000000000001			1	Col I	2180.39501953125	301.9862060546875	83.60588073730469	300	85.09999999999995
10	300	96.89999999999999			9	Col I	1917.8831787109375	375.14276123046875	132.87686157226562	300	80.7000000000001
11	300	81.30000000000004			17	Col I	2178.0859375	806.1314697265625	72.3534927368164	300	112.20000000000007
12	200	67.70000000000009			5	prr7,9 I	2514.615478515625	299.6667175292969	70.3182601928711	200	67.80000000000008
13	400	105.89999999999999			13	prr7,9 I	1365.0078125	1249.138671875	116.79225158691406	400	105.89999999999999
14	200	50.69999999999997			21	prr7,9 I	1654.855712890625	1042.51416015625	116.10993957519531	400	101.40000000000005
15	200	54.59999999999998			Standard	1	1645.3131103515625	1719.94091796875	2256.296630859375
16	400	101.89999999999999			2	Col II	2157.2509765625	176.75811767578125	54.220611572265625	200	55.79999999999996
17	300	112.20000000000007			10	Col II	1865.593994140625	616.8743286132812	59.84711456298828	300	96.89999999999999
18	200	76.5999999999999			18	Col II	2377.778564453125	465.9914245605469	59.2650146484375	200	76.5999999999999
19	300	99.50000000000014			6	prr7,9 II	2416.207275390625	603.93408203125	58.665550231933594	200	62.699999999999974
20	300	102.7999999999999			14	prr7,9 II	2289.3037109375	526.7593383789062	127.31964874267578	200	50.69999999999997
21	400	101.40000000000005			22	prr7,9 II	1362.8040771484375	1686.056884765625	129.24539184570312	400	123.19999999999997
22	400	123.19999999999997			Standard	0.5	808.2450561523438	831.448486328125	1122.8255615234375
23	400	109.20000000000007			3	Col III	2016.7115478515625	173.78077697753906	65.31547546386719	200	46.499999999999986
24	300	73.6			11	Col III	1981.958740234375	174.57398986816406	21.63164520263672	300	81.30000000000004
					19	Col III	2064.210693359375	473.5988464355469	64.27493286132812	300	99.50000000000014
					7	prr7,9 III	2498.947509765625	391.9966735839844	105.10193634033203	200	55.30000000000002
	Derivatization				15	prr7,9 III	2427.84375	588.3184204101562	63.29181671142578	200	54.59999999999998
	25 µL				23	prr7,9 III	1696.642333984375	1158.29	123.79141235351562	400	109.20000000000007
	injection	injection			Standard	1	1606.5908203125	1679.7308349609375	2153.149169921875
	20 µL				4	Col IV	2237.2646484375	125.17689514160156	43.60393142700195	200	61.40000000000001
					12	Col IV	2196.937255859375	269.585	42.28960418701172	200	67.70000000000009
					20	Col IV	2176.550048828125	417.441	73.04740905761719	300	102.7999999999999
					8	prr7,9 IV	2290.056396484375	659.33	345.0472412109375	300	75.6
					16	prr7,9 IV	1518.505859375	1229.6	95.14126586914062	400	101.89999999999999
					24	prr7,9 IV	2028.2176513671875	424.313	69.16194915771484	300	73.6
					Standard	0.25	447.85986328125	458.4064636230469	788.3421020507812
					Standard	0.03125	98.93505859375	100.81101989746094	217.923583984375



Sample analysis order		TDP	TMP	THIA	Extraction volume	Mass
1	Col I	2180.39501953125	301.9862060546875	83.60588073730469	3.0E-4	0.08509999999999995
2	Col II	2157.2509765625	176.75811767578125	54.220611572265625	1.9999999999999998E-4	0.05579999999999996
3	Col III	2016.7115478515625	173.78077697753906	65.31547546386719	1.9999999999999998E-4	0.046499999999999986
4	Col IV	2237.2646484375	125.17689514160156	43.60393142700195	1.9999999999999998E-4	0.06140000000000002
5	prr7,9 I	2514.615478515625	299.6667175292969	70.3182601928711	1.9999999999999998E-4	0.06780000000000008
6	prr7,9 II	2416.207275390625	603.93408203125	58.665550231933594	1.9999999999999998E-4	0.06269999999999998
7	prr7,9 III	2498.947509765625	391.9966735839844	105.10193634033203	1.9999999999999998E-4	0.05530000000000002				pmol	TDP	TMP	Thia
8	prr7,9 IV	2290.056396484375	659.33	345.0472412109375	3.0E-4	0.0756				3.125E-14	98.93505859375	100.81101989746094	217.923583984375
9	Col I	1917.8831787109375	375.14276123046875	132.87686157226562	3.0E-4	0.0807000000000001				6.25E-14	252.35	237.66	289.42
10	Col II	1865.593994140625	616.8743286132812	59.84711456298828	3.0E-4	0.0969				1.25E-13	309.66	318.61	576.96
11	Col III	1981.958740234375	174.57398986816406	21.63164520263672	3.0E-4	0.08130000000000004				2.5E-13	502.94	515.39	955.81
12	Col IV	2196.937255859375	269.585	42.28960418701172	1.9999999999999998E-4	0.0677000000000001				5.0E-13	850.95	859.87	1269.7
13	prr7,9 I	1365.0078125	1249.138671875	116.79225158691406	3.9999999999999996E-4	0.1059				1.0E-12	1621.6	1673.7	2243.1
14	prr7,9 II	2289.3037109375	526.7593383789062	127.31964874267578	1.9999999999999998E-4	0.05069999999999997				2.0E-12	3490.9	3645	4747
15	prr7,9 III	2427.84375	588.3184204101562	63.29181671142578	1.9999999999999998E-4	0.05459999999999998				4.0E-12	6814.1	7077	9161
16	prr7,9 IV	1518.505859375	1229.6	95.14126586914062	3.9999999999999996E-4	0.10189999999999999				Slope	1.685774878178111E15	1.7555770182597208E15	2.2371156417932975E15
17	Col I	2178.0859375	806.1314697265625	72.3534927368164	3.0E-4	0.11220000000000008				b	63.49	54.786	204.24
18	Col II	2377.778564453125	465.9914245605469	59.2650146484375	1.9999999999999998E-4	0.07659999999999989
19	Col III	2064.210693359375	473.5988464355469	64.27493286132812	3.0E-4	0.09950000000000014
20	Col IV	2176.550048828125	417.441	73.04740905761719	3.0E-4	0.1027999999999999
21	prr7,9 I	1654.855712890625	1042.51416015625	116.10993957519531	3.9999999999999996E-4	0.10140000000000005
22	prr7,9 II	1362.8040771484375	1686.056884765625	129.24539184570312	3.9999999999999996E-4	0.12319999999999998
23	prr7,9 III	1696.642333984375	1158.29	123.79141235351562	3.9999999999999996E-4	0.10920000000000007
24	prr7,9 IV	2028.2176513671875	424.313	69.16194915771484	3.0E-4	0.0736


Sample analysis order	pmol injected	TDP	TMP	THIA			Sample analysis order	pmol/µL	TDP	TMP	THIA
1	Col I	1.2557459758915614E-12	1.4080852248779928E-13	4.180294036865234E-14		27 EN	1	Col I	2.511491951783123E-7	2.8161704497559855E-8	8.360588073730468E-9
2	Col II	1.2420169523615854E-12	6.947693915285502E-14	2.7110305786132812E-14			2	Col II	2.4840339047231705E-7	1.3895387830571003E-8	5.422061157226562E-9
3	Col III	1.1586491014520826E-12	6.778100632434624E-14	3.265773773193359E-14			3	Col III	2.3172982029041653E-7	1.3556201264869248E-8	6.531547546386718E-9
4	Col IV	1.289480983835037E-12	4.009558931876374E-14	2.1801965713500978E-14			4	Col IV	2.5789619676700736E-7	8.019117863752749E-9	4.360393142700195E-9
5	prr7,9 I	1.4540052234997483E-12	1.3948731099934524E-13	3.515913009643555E-14			5	prr7,9 I	2.908010446999496E-7	2.7897462199869047E-8	7.0318260192871094E-9
6	prr7,9 II	1.3956295741773702E-12	3.1280204532160763E-13	2.93327751159668E-14			6	prr7,9 II	2.79125914835474E-7	6.256040906432153E-8	5.8665550231933595E-9
7	prr7,9 III	1.4447109998446106E-12	1.9207968096908483E-13	5.2550968170166016E-14			7	prr7,9 III	2.889421999689221E-7	3.8415936193816965E-8	1.0510193634033203E-8
8	prr7,9 IV	1.3207969968627843E-12	3.443562963698831E-13	1.7252362060546875E-13			8	prr7,9 IV	2.6415939937255685E-7	6.887125927397661E-8	3.450472412109375E-8
9	Col I	1.1000242100624132E-12	1.824794685157328E-13	6.643843078613281E-14		28 EN	9	Col I	2.2000484201248262E-7	3.6495893703146554E-8	1.328768615722656E-8
10	Col II	1.0690063171947585E-12	3.2017298174162225E-13	2.992355728149414E-14			10	Col II	2.138012634389517E-7	6.403459634832445E-8	5.9847114562988275E-9
11	Col III	1.1380337701483285E-12	6.823283092809465E-14	1.081582260131836E-14			11	Col III	2.2760675402966568E-7	1.364656618561893E-8	2.1631645202636718E-9
12	Col IV	1.2655588142143173E-12	1.2235236492952458E-13	2.114480209350586E-14			12	Col IV	2.5311176284286343E-7	2.4470472985904917E-8	4.228960418701171E-9
13	prr7,9 I	7.72059086505433E-13	6.803191540174895E-13	5.839612579345703E-14			13	prr7,9 I	1.5441181730108656E-7	1.360638308034979E-7	1.1679225158691405E-8
14	prr7,9 II	1.3203505045365442E-12	2.6884228573849006E-13	6.365982437133789E-14			14	prr7,9 II	2.640701009073088E-7	5.376845714769801E-8	1.2731964874267577E-8
15	prr7,9 III	1.4025323194727273E-12	3.039071569409353E-13	3.164590835571289E-14			15	prr7,9 III	2.8050646389454546E-7	6.078143138818706E-8	6.329181671142578E-9
16	prr7,9 IV	8.63113976966781E-13	6.691896668621105E-13	4.757063293457031E-14			16	prr7,9 IV	1.726227953933562E-7	1.338379333724221E-7	9.514126586914061E-9
17	Col I	1.254376230701299E-12	4.2797636441570696E-13	3.6176746368408206E-14		28 ED	17	Col I	2.5087524614025976E-7	8.559527288314139E-8	7.235349273681641E-9
18	Col II	1.3728337006387685E-12	2.3422807446418336E-13	2.963250732421875E-14			18	Col II	2.745667401277537E-7	4.684561489283667E-8	5.92650146484375E-9
19	Col III	1.1868255478582284E-12	2.3856136306153646E-13	3.213746643066406E-14			19	Col III	2.3736510957164565E-7	4.771227261230729E-8	6.427493286132813E-9
20	Col IV	1.2534651430514847E-12	2.065731074330734E-13	3.652370452880859E-14			20	Col IV	2.5069302861029693E-7	4.1314621486614676E-8	7.304740905761718E-9
21	prr7,9 I	9.439965759903138E-13	5.626230862462367E-13	5.805496978759765E-14			21	prr7,9 I	1.8879931519806277E-7	1.1252461724924733E-7	1.161099395751953E-8
22	prr7,9 II	7.707518328619666E-13	9.291935744195838E-13	6.462269592285156E-14			22	prr7,9 II	1.541503665723933E-7	1.8583871488391673E-7	1.2924539184570312E-8
23	prr7,9 III	9.687843585315452E-13	6.285705432017378E-13	6.189570617675781E-14			23	prr7,9 III	1.9375687170630903E-7	1.2571410864034754E-7	1.2379141235351562E-8
24	prr7,9 IV	1.16547451074283E-12	2.1048748995718058E-13	3.458097457885742E-14			24	prr7,9 IV	2.3309490214856598E-7	4.209749799143611E-8	6.9161949157714844E-9

Sample analysis order	nmol/gFW	TDP	TMP	THIA
1	Col I	885.3673155522177	99.27745416296075	29.473283456159127			Mean		TDP	TMP	THIA
2	Col II	890.3347328756889	49.80425745724377	19.43391095780131			27 EN	Col	903.1105514312983	58.377214678324826	22.800776756334205
3	Col III	996.6873990985658	58.30624199943764	28.09267761886761			28 ED	Col	766.8519602323545	114.14230855984623	22.100128563367562
4	Col IV	840.0527581987208	26.12090509365715	14.203234992508774			28 EN	Col	708.7355014572627	153.90018407206077	18.87911546319028
5	prr7,9 I	857.8201908553074	82.29339881967259	20.742849614416226			27 EN	prr7,9	960.3561214730375	173.5208146716876	53.59775060445553
6	prr7,9 II	890.3537953284659	199.554733857485	18.71309417286558			28 ED	prr7,9	832.5114124761428	368.51248577692866	38.71740225546809
7	prr7,9 III	1044.9989148966433	138.93647809698714	38.01155021350162			28 EN	prr7,9	726.2764745957589	419.8352113851851	40.32534466004294
8	prr7,9 IV	1048.2515848117334	273.2986479126056	136.92350841703868
9	Col I	817.8618662174064	135.6724672979424	49.39660281496856			STDEV		TDP	TMP	THIA
10	Col II	661.9234162196647	198.24952429821812	18.528518440553643			27 EN	Col	33.180008622075476	15.239203092020402	3.626063867843288
11	Col III	839.8773211426773	50.356332788261696	7.982156901341957			28 ED	Col	40.11419122603355	33.36385488473636	9.351753009264181
12	Col IV	747.7452373496694	72.29090985496272	12.4932360966061			28 EN	Col	13.156048540266871	25.54300793249725	1.2250361762140936
13	prr7,9 I	583.2363259719983	513.9332608252989	44.114164905349966			27 EN	prr7,9	50.252659703550506	40.97956675158741	28.11063756404265
14	prr7,9 II	1041.6966505219289	212.10436744654058	50.22471350795891			28 ED	prr7,9	118.28920963749397	87.31786360652637	5.807422031023975
15	prr7,9 III	1027.4962047419249	222.64260581753507	23.18381564521091			28 EN	prr7,9	92.05967843469897	90.15789119686292	4.134412699440083
16	prr7,9 IV	677.6164686687191	525.36970901834	37.346914963352546
17	Col I	670.7894281825121	228.86436599770408	19.345853672945548			Fold vs Col		TDP	TMP	THIA
18	Col II	716.8844389758591	122.31231042516119	15.473894164082918			27 EN	Col
19	Col III	715.6736972009405	143.85609832856449	19.379376742108956			28 ED	Col
20	Col IV	731.5944414697387	120.56796153681334	21.31733727362371			28 EN	Col
21	prr7,9 I	744.7704741540934	443.88409171300697	45.802737505007975			27 EN	prr7,9	1.0633871124094536	2.9724065395691963	2.3506984510764846
22	prr7,9 II	500.4882031571212	603.3724509218076	41.96278956029323			28 ED	prr7,9	1.0856220700327788	3.2285354171167215	1.7519084626342294
23	prr7,9 III	709.7321307923403	460.4912404408331	45.34483968993242			28 EN	prr7,9	1.0247496747410416	2.7279708202857345	2.135976377636316
24	prr7,9 IV	950.1150902794808	171.59306246509283	28.191011884938113



Sample analysis order		THIA	Extraction volume	Mass
1	Col I	285.237	3.0E-4	0.08509999999999995
2	Col II	121.45	1.9999999999999998E-4	0.05579999999999996
3	Col III	160.685	1.9999999999999998E-4	0.046499999999999986
4	Col IV	150.074	1.9999999999999998E-4	0.06140000000000002
5	prr7,9 I	272.37	1.9999999999999998E-4	0.06780000000000008
6	prr7,9 II	242.808	1.9999999999999998E-4	0.06269999999999998
7	prr7,9 III	164.77	1.9999999999999998E-4	0.05530000000000002				pmol	Thia
8	prr7,9 IV	855.881	3.0E-4	0.0756				6.25E-14	291.3258
9	Col I	203.8	3.0E-4	0.0807000000000001				1.25E-13	464.7634
10	Col II	156.916	3.0E-4	0.0969				2.5E-13	711.93347
11	Col III	135.305	3.0E-4	0.08130000000000004				5.0E-13	964.34528
12	Col IV	162.112	1.9999999999999998E-4	0.0677000000000001				1.0E-12	1763.112
13	prr7,9 I	330.51	3.9999999999999996E-4	0.1059				2.0E-12	4222.5888
14	prr7,9 II	166.392	1.9999999999999998E-4	0.05069999999999997				Slope	1.9906732241935485E15
15	prr7,9 III	288.228	1.9999999999999998E-4	0.05459999999999998				b	256.55
16	prr7,9 IV	273.176	3.9999999999999996E-4	0.10189999999999999
17	Col I	324.4	3.0E-4	0.11220000000000008
18	Col II	187.35	1.9999999999999998E-4	0.07659999999999989
19	Col III	164.641	3.0E-4	0.09950000000000014
20	Col IV	206.925	3.0E-4	0.1027999999999999
21	prr7,9 I	307.41	3.9999999999999996E-4	0.10140000000000005
22	prr7,9 II	445.663	3.9999999999999996E-4	0.12319999999999998
23	prr7,9 III	166.888	3.9999999999999996E-4	0.10920000000000007
24	prr7,9 IV	184.335	3.0E-4	0.0736


Sample analysis order	pmol injected	THIA			Sample analysis order	pmol/µL	THIA
1	Col I	1.4261850000000002E-13		27 EN	1	Col I	1.140948E-8
2	Col II	6.0725E-14			2	Col II	4.8579999999999996E-9
3	Col III	8.03425E-14			3	Col III	6.4274E-9
4	Col IV	7.503700000000001E-14			4	Col IV	6.0029600000000005E-9
5	prr7,9 I	1.36185E-13			5	prr7,9 I	1.0894799999999998E-8
6	prr7,9 II	1.21404E-13			6	prr7,9 II	9.71232E-9
7	prr7,9 III	8.238500000000001E-14			7	prr7,9 III	6.5908E-9
8	prr7,9 IV	4.279405E-13			8	prr7,9 IV	3.423524E-8
9	Col I	1.019E-13		28 EN	9	Col I	8.151999999999999E-9
10	Col II	7.8458E-14			10	Col II	6.276639999999999E-9
11	Col III	6.76525E-14			11	Col III	5.4122E-9
12	Col IV	8.1056E-14			12	Col IV	6.4844799999999995E-9
13	prr7,9 I	1.6525499999999999E-13			13	prr7,9 I	1.3220399999999999E-8
14	prr7,9 II	8.3196E-14			14	prr7,9 II	6.65568E-9
15	prr7,9 III	1.4411400000000002E-13			15	prr7,9 III	1.152912E-8
16	prr7,9 IV	1.3658799999999998E-13			16	prr7,9 IV	1.0927039999999999E-8
17	Col I	1.6219999999999998E-13		28 ED	17	Col I	1.2975999999999998E-8
18	Col II	9.3675E-14			18	Col II	7.493999999999999E-9
19	Col III	8.23205E-14			19	Col III	6.58564E-9
20	Col IV	1.034625E-13			20	Col IV	8.277E-9
21	prr7,9 I	1.5370500000000003E-13			21	prr7,9 I	1.2296400000000002E-8
22	prr7,9 II	2.228315E-13			22	prr7,9 II	1.782652E-8
23	prr7,9 III	8.344400000000001E-14			23	prr7,9 III	6.6755200000000005E-9
24	prr7,9 IV	9.216750000000001E-14			24	prr7,9 IV	7.3734E-9

Sample analysis order	nmol/gFW	THIA
1	Col I	40.22143360752058					THIA
2	Col II	17.412186379928322			27 EN	Col	26.207991701355937
3	Col III	27.644731182795706			28 ED	Col	22.216221609963032
4	Col IV	19.55361563517915			28 EN	Col	24.568159266491413
5	prr7,9 I	32.138053097345086			27 EN	prr7,9	55.70224879793622
6	prr7,9 II	30.980287081339725			28 ED	prr7,9	40.32873922161233
7	prr7,9 III	23.83652802893308			28 EN	prr7,9	40.22297358543073
8	prr7,9 IV	135.854126984127
9	Col I	30.3048327137546					THIA
10	Col II	19.432321981424142			27 EN	Col	5.16468526370469
11	Col III	19.971217712177108			28 ED	Col	2.701505439732336
12	Col IV	19.15651403249628			28 EN	Col	3.534897659186522
13	prr7,9 I	49.935410764872515			27 EN	prr7,9	26.780270173884016
14	prr7,9 II	26.2551479289941			28 ED	prr7,9	5.004580445280961
15	prr7,9 III	42.2312087912088			28 EN	prr7,9	7.812557334225081
16	prr7,9 IV	42.893189401373895
17	Col I	34.695187165775366
18	Col II	19.566579634464773
19	Col III	19.856201005025095
20	Col IV	24.15466926070041
21	prr7,9 I	48.50650887573963
22	prr7,9 II	57.8783116883117
23	prr7,9 III	24.452454212454196
24	prr7,9 IV	30.054619565217386

	Sample analysis order	pmol/µL	TDP	TMP	THIA
27 EN	1	Col I	885.3673155522177	99.27745416296075	29.473283456159127
	2	Col II	890.3347328756889	49.80425745724377	19.43391095780131			TDP	TMP	Thia			TDP	TMP	Thia
	3	Col III	996.6873990985658	58.30624199943764	28.09267761886761	27EN	Col vs prr7.9	0.3784950101359991	0.03887304751915922	0.31894335633099896	27EN	Col vs prr7.9	n.s	*	n.s
	4	Col IV	840.0527581987208	26.12090509365715	14.203234992508774	28ED	Col vs prr7.9	0.6179695046170415	0.034584532576066675	0.18190213222393758	28ED	Col vs prr7.9	n.s	*	n.s
	5	prr7,9 I	857.8201908553074	82.29339881967259	20.742849614416226	28EN	Col vs prr7.9	0.856606669838744	0.029646233508172554	0.002517983320163621	28EN	Col vs prr7.9	n.s	*	**
	6	prr7,9 II	890.3537953284659	199.554733857485	18.71309417286558	Col	27 EN vs 28 EN	0.0397230310306063	0.17923948406921422	0.9465796126527969	Col	27 EN vs 28 EN	*	n.s	n.s
	7	prr7,9 III	1044.9989148966433	138.93647809698714	38.01155021350162	prr7.9	27 EN vs 28 EN	0.3582725251207178	0.08970590900428527	0.6227325689414196	prr7.9	27 EN vs 28 EN	n.s	n.s	n.s
	8	prr7,9 IV	1048.2515848117334	273.2986479126056	136.92350841703868	Col	28 EN vs 28 ED	0.21778917569917583	0.3805817643529822	0.7443616614354294	Col	28 EN vs 28 ED	n.s	n.s	n.s
28 EN	9	Col I	817.8618662174064	135.6724672979424	49.39660281496856	prr7.9	28 EN vs 28 ED	0.5050727905942308	0.6967992204231616	0.8290329831982349	prr7.9	28 EN vs 28 ED	n.s	n.s	n.s
	10	Col II	661.9234162196647	198.24952429821812	18.528518440553643	Col	27 EN vs 28 ED	0.0015937499093712326	0.018331131421305904	0.3450625364371603	Col	27 EN vs 28 ED	**	*	n.s
	11	Col III	839.8773211426773	50.356332788261696	7.982156901341957	prr7.9	27 EN vs 28 ED	0.06709678940629217	0.047343595565421566	0.6568931221269751	prr7.10	27 EN vs 28 ED	n.s	*	n.s
	12	Col IV	747.7452373496694	72.29090985496272	12.4932360966061
	13	prr7,9 I	583.2363259719983	513.9332608252989	44.114164905349966
	14	prr7,9 II	1041.6966505219289	212.10436744654058	50.22471350795891
	15	prr7,9 III	1027.4962047419249	222.64260581753507	23.18381564521091
	16	prr7,9 IV	677.6164686687191	525.36970901834	37.346914963352546
28 ED	17	Col I	670.7894281825121	228.86436599770408	19.345853672945548
	18	Col II	716.8844389758591	122.31231042516119	15.473894164082918
	19	Col III	715.6736972009405	143.85609832856449	19.379376742108956
	20	Col IV	731.5944414697387	120.56796153681334	21.31733727362371
	21	prr7,9 I	744.7704741540934	443.88409171300697	45.802737505007975
	22	prr7,9 II	500.4882031571212	603.3724509218076	41.96278956029323
	23	prr7,9 III	709.7321307923403	460.4912404408331	45.34483968993242
	24	prr7,9 IV	950.1150902794808	171.59306246509283	28.191011884938113

	Sample analysis order	pmol/µL	THIA
27 EN	1	Col I	100.55358401880146
	2	Col II	43.530465949820815			Thia			Thia
	3	Col III	69.11182795698926	27EN	Col vs prr7.9	0.3210491931812907	27EN	Col vs prr7.9	n.s
	4	Col IV	48.884039087947876	28ED	Col vs prr7.9	0.018960465831258648	28ED	Col vs prr7.9	*
	5	prr7,9 I	80.34513274336271	28EN	Col vs prr7.9	0.11769982614260949	28EN	Col vs prr7.9	n.s
	6	prr7,9 II	77.4507177033493	Col	27 EN vs 28 EN	0.5189961799445153	Col	27 EN vs 28 EN	n.s
	7	prr7,9 III	59.5913200723327	prr7.9	27 EN vs 28 EN	0.5930067610455964	prr7.9	27 EN vs 28 EN	n.s
	8	prr7,9 IV	339.6353174603173	Col	28 EN vs 28 ED	0.6160276879003956	Col	28 EN vs 28 ED	n.s
28 EN	9	Col I	75.76208178438651	prr7.9	28 EN vs 28 ED	0.9912742373838004	prr7.9	28 EN vs 28 ED	n.s
	10	Col II	48.58080495356035	Col	27 EN vs 28 ED	0.802073365028804	Col	27 EN vs 28 ED	n.s
	11	Col III	49.92804428044277	prr7.9	27 EN vs 28 ED	0.5990358884307454	prr7.10	27 EN vs 28 ED	n.s
	12	Col IV	47.89128508124068
	13	prr7,9 I	124.83852691218128
	14	prr7,9 II	65.63786982248523
	15	prr7,9 III	105.578021978022
	16	prr7,9 IV	107.23297350343472
28 ED	17	Col I	86.73796791443841
	18	Col II	48.91644908616194
	19	Col III	49.640502512562726
	20	Col IV	60.386673151751026
	21	prr7,9 I	121.26627218934907
	22	prr7,9 II	144.6957792207792
	23	prr7,9 III	61.13113553113548
	24	prr7,9 IV	75.13654891304348

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9.3456598122228	28.0082702636719	28.5896241664887	30.3724348545075	30.3900480270386	9.3456598122228	178.0082702636719	53.589624166488704	30.3724348545075	105.3900480270386
9.3528598583031	28.0036509037018	28.5839974880219	30.3413987159729	30.3627848625183	9.3528598583031	178.0036509037018	53.5839974880219	30.3413987159729	105.3627848625183
9.36005990438339	28.000682592392	28.578794002533	30.3166389465332	30.3377687931061	9.36005990438339	178.000682592392	53.578794002533	30.3166389465332	105.33776879310611
9.36725995046369	27.9991567134857	28.5749733448029	30.2982866764069	30.3152859210968	9.36725995046369	177.9991567134857	53.5749733448029	30.2982866764069	105.3152859210968
9.37445999654398	27.999085187912	28.5733282566071	30.2859604358673	30.2957892417908	9.37445999654398	177.999085187912	53.5733282566071	30.2859604358673	105.2957892417908
9.38166004262427	28.000819683075	28.5743236541748	30.2788376808167	30.2799046039582	9.38166004262427	178.000819683075	53.574323654174805	30.2788376808167	105.2799046039582
9.38886008870457	28.0048906803131	28.5780191421509	30.275821685791	30.2683234214783	9.38886008870457	178.0048906803131	53.5780191421509	30.275821685791	105.2683234214783
9.39606013478486	28.0117154121399	28.5840034484863	30.2758157253266	30.2616715431214	9.39606013478486	178.0117154121399	53.5840034484863	30.2758157253266	105.2616715431214
9.40326018086516	28.0215084552765	28.5915613174439	30.2779316902161	30.2603423595429	9.40326018086516	178.0215084552765	53.591561317443904	30.2779316902161	105.2603423595429
9.41046022694546	28.0342936515808	28.5999476909638	30.2817165851593	30.2644014358521	9.41046022694546	178.0342936515808	53.5999476909638	30.2817165851593	105.26440143585211
9.41766027302575	28.0502438545227	28.6086678504944	30.2872180938721	30.2736282348633	9.41766027302575	178.0502438545227	53.6086678504944	30.2872180938721	105.2736282348633
9.42486031910604	28.0699074268341	28.6174833774567	30.2949607372284	30.2875876426697	9.42486031910604	178.0699074268341	53.6174833774567	30.2949607372284	105.2875876426697
9.43206036518634	28.0942559242249	28.6262392997742	30.3057193756104	30.3056597709656	9.43206036518634	178.0942559242249	53.6262392997742	30.3057193756104	105.3056597709656
9.43926041126663	28.1244277954102	28.6346316337586	30.3202331066132	30.3271532058716	9.43926041126663	178.1244277954102	53.6346316337586	30.3202331066132	105.3271532058716
9.44646045734693	28.1614482402802	28.6422610282898	30.3390562534332	30.3515613079071	9.44646045734693	178.1614482402802	53.642261028289795	30.3390562534332	105.3515613079071
9.45366050342722	28.2059907913208	28.648841381073	30.3625524044037	30.3788661956787	9.45366050342722	178.2059907913208	53.648841381073	30.3625524044037	105.3788661956787
9.46086054950752	28.2581746578217	28.6543726921082	30.3910553455353	30.4095685482025	9.46086054950752	178.2581746578217	53.6543726921082	30.3910553455353	105.4095685482025
9.46806059558781	28.3176839351654	28.658938407898	30.4247736930847	30.4444968700409	9.46806059558781	178.3176839351654	53.658938407898	30.4247736930847	105.4444968700409
9.47526064166811	28.3836424350739	28.66250872612	30.4636061191559	30.4844319820404	9.47526064166811	178.3836424350739	53.662508726119995	30.4636061191559	105.4844319820404
9.4824606877484	28.4545004367829	28.6649584770203	30.5070340633393	30.5300056934357	9.4824606877484	178.4545004367829	53.6649584770203	30.5070340633393	105.5300056934357
9.4896607338287	28.5279929637909	28.6662936210633	30.5541872978211	30.5817008018494	9.4896607338287	178.5279929637909	53.6662936210633	30.5541872978211	105.5817008018494
9.49686077990899	28.6014258861542	28.666889667511	30.6041538715363	30.6396961212158	9.49686077990899	178.6014258861542	53.666889667511	30.6041538715363	105.63969612121579
9.50406082598929	28.6723256111145	28.6674499511719	30.6561410427094	30.7034015655518	9.50406082598929	178.6723256111145	53.6674499511719	30.6561410427094	105.7034015655518
9.51126087206958	28.7389039993286	28.6685109138489	30.7093441486359	30.7711005210877	9.51126087206958	178.7389039993286	53.6685109138489	30.7093441486359	105.7711005210877
9.51846091814988	28.8000524044037	28.6701858043671	30.7626962661743	30.8399856090546	9.51846091814988	178.8000524044037	53.6701858043671	30.7626962661743	105.8399856090546
9.52566096423017	28.854900598526	28.6721825599671	30.8148384094238	30.9067070484162	9.52566096423017	178.854900598526	53.6721825599671	30.8148384094238	105.9067070484162
9.53286101031047	28.9025187492371	28.6741256713867	30.8643519878388	30.9680461883545	9.53286101031047	178.9025187492371	53.674125671386705	30.8643519878388	105.96804618835449
9.54006105639076	28.9418816566467	28.6756873130799	30.9099614620209	31.0212194919586	9.54006105639076	178.9418816566467	53.675687313079905	30.9099614620209	106.0212194919586
9.54726110247106	28.9719581604004	28.6764442920685	30.9506237506867	31.064110994339	9.54726110247106	178.9719581604004	53.676444292068496	30.9506237506867	106.064110994339
9.55446114855135	28.99169921875	28.6758482456207	30.9854507446289	31.095439195633	9.55446114855135	178.99169921875	53.6758482456207	30.9854507446289	106.09543919563299
9.56166119463165	28.9997935295105	28.673529624939	31.0135424137116	31.1149179935456	9.56166119463165	178.9997935295105	53.673529624939	31.0135424137116	106.1149179935456
9.56886124071194	28.9948165416718	28.6697030067444	31.0340225696564	31.1232924461365	9.56886124071194	178.9948165416718	53.6697030067444	31.0340225696564	106.1232924461365
9.57606128679224	28.9757490158081	28.6652326583863	31.0461223125458	31.1220824718476	9.57606128679224	178.9757490158081	53.6652326583863	31.0461223125458	106.1220824718476
9.58326133287253	28.9425134658814	28.6612629890442	31.0494363307953	31.1130583286286	9.58326133287253	178.9425134658814	53.661262989044204	31.0494363307953	106.1130583286286
9.59046137895283	28.8963198661804	28.6587417125702	31.0440540313721	31.0977458953858	9.59046137895283	178.8963198661804	53.658741712570205	31.0440540313721	106.0977458953858
9.59766142503312	28.8394451141358	28.6583244800568	31.0304343700409	31.0772478580475	9.59766142503312	178.8394451141358	53.658324480056805	31.0304343700409	106.0772478580475
9.60486147111342	28.7749111652374	28.6604344844818	31.009191274643	31.052178144455	9.60486147111342	178.7749111652374	53.6604344844818	31.009191274643	106.052178144455
9.61206151719371	28.7061035633087	28.6651611328125	30.9808433055878	31.0228526592255	9.61206151719371	178.70610356330872	53.6651611328125	30.9808433055878	106.02285265922549
9.61926156327401	28.6364138126374	28.6719501018524	30.9459030628205	30.9894740581513	9.61926156327401	178.6364138126374	53.6719501018524	30.9459030628205	105.9894740581513
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