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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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8.62565520419331 | 28.575736284256 | 29.1435182094574 | 31.156075000763 | 31.170517206192 | 8.62565520419331 | 178.575736284256 | 54.1435182094574 | 31.156075000763 | 106.170517206192 | |
8.63285525027361 | 28.5471737384796 | 29.1551947593689 | 31.1694324016571 | 31.2233150005341 | 8.63285525027361 | 178.54717373847961 | 54.1551947593689 | 31.1694324016571 | 106.2233150005341 | |
8.6400552963539 | 28.5160660743714 | 29.16499376297 | 31.1878979206085 | 31.2770366668701 | 8.6400552963539 | 178.5160660743714 | 54.16499376297 | 31.1878979206085 | 106.2770366668701 | |
8.64725534243419 | 28.4839570522309 | 29.1716575622559 | 31.2122821807862 | 31.3313066959381 | 8.64725534243419 | 178.4839570522309 | 54.1716575622559 | 31.2122821807862 | 106.3313066959381 | |
8.65445538851449 | 28.4523725509644 | 29.1740775108338 | 31.2429010868073 | 31.3858985900879 | 8.65445538851449 | 178.4523725509644 | 54.1740775108338 | 31.2429010868073 | 106.3858985900879 | |
8.66165543459479 | 28.4225761890412 | 29.1712701320648 | 31.2793970108032 | 31.4404308795929 | 8.66165543459479 | 178.4225761890412 | 54.171270132064805 | 31.2793970108032 | 106.4404308795929 | |
8.66885548067508 | 28.3953845500946 | 29.1627526283264 | 31.3206553459168 | 31.4941048622132 | 8.66885548067508 | 178.3953845500946 | 54.1627526283264 | 31.3206553459168 | 106.49410486221319 | |
8.67605552675538 | 28.3710598945618 | 29.1487812995911 | 31.3647150993347 | 31.5456032752991 | 8.67605552675538 | 178.3710598945618 | 54.1487812995911 | 31.3647150993347 | 106.5456032752991 | |
8.68325557283567 | 28.3493518829346 | 29.1303992271424 | 31.4089834690094 | 31.5932631492615 | 8.68325557283567 | 178.3493518829346 | 54.130399227142405 | 31.4089834690094 | 106.5932631492615 | |
8.69045561891596 | 28.3296883106232 | 29.1090488433838 | 31.4507722854614 | 31.6354930400849 | 8.69045561891596 | 178.3296883106232 | 54.1090488433838 | 31.4507722854614 | 106.6354930400849 | |
8.69765566499626 | 28.3115029335022 | 29.0860950946808 | 31.4877450466156 | 31.671005487442 | 8.69765566499626 | 178.3115029335022 | 54.0860950946808 | 31.4877450466156 | 106.671005487442 | |
8.70485571107655 | 28.2943546772003 | 29.0626525878906 | 31.5182685852051 | 31.6988348960877 | 8.70485571107655 | 178.2943546772003 | 54.0626525878906 | 31.5182685852051 | 106.6988348960877 | |
8.71205575715685 | 28.2780170440674 | 29.0398716926575 | 31.5414845943451 | 31.718373298645 | 8.71205575715685 | 178.2780170440674 | 54.0398716926575 | 31.5414845943451 | 106.718373298645 | |
8.71925580323715 | 28.2624542713165 | 29.0190517902374 | 31.5572917461395 | 31.7292153835297 | 8.71925580323715 | 178.2624542713165 | 54.0190517902374 | 31.5572917461395 | 106.72921538352969 | |
8.72645584931744 | 28.2477796077728 | 29.0014088153839 | 31.5663456916809 | 31.7312002182007 | 8.72645584931744 | 178.2477796077728 | 54.0014088153839 | 31.5663456916809 | 106.7312002182007 | |
8.73365589539773 | 28.2342374324799 | 28.9876937866211 | 31.5699577331543 | 31.7244231700897 | 8.73365589539773 | 178.23423743247992 | 53.9876937866211 | 31.5699577331543 | 106.7244231700897 | |
8.74085594147803 | 28.2222330570221 | 28.9780855178833 | 31.5697968006134 | 31.7092537879944 | 8.74085594147803 | 178.2222330570221 | 53.9780855178833 | 31.5697968006134 | 106.7092537879944 | |
8.74805598755832 | 28.2121241092682 | 28.9722919464112 | 31.5674126148224 | 31.6861093044281 | 8.74805598755832 | 178.2121241092682 | 53.972291946411204 | 31.5674126148224 | 106.6861093044281 | |
8.75525603363862 | 28.2039642333985 | 28.9697945117951 | 31.563937664032 | 31.6553592681885 | 8.75525603363862 | 178.2039642333985 | 53.9697945117951 | 31.563937664032 | 106.6553592681885 | |
8.76245607971891 | 28.1975030899048 | 28.9698004722595 | 31.5598607063294 | 31.6172599792481 | 8.76245607971891 | 178.19750308990479 | 53.9698004722595 | 31.5598607063294 | 106.6172599792481 | |
8.76965612579921 | 28.1922221183777 | 28.9709985256195 | 31.5551340579987 | 31.5722525119782 | 8.76965612579921 | 178.19222211837769 | 53.9709985256195 | 31.5551340579987 | 106.5722525119782 | |
8.7768561718795 | 28.1876146793366 | 28.9717733860016 | 31.5492928028107 | 31.5214216709137 | 8.7768561718795 | 178.1876146793366 | 53.9717733860016 | 31.5492928028107 | 106.5214216709137 | |
8.7840562179598 | 28.1833708286286 | 28.9704859256745 | 31.5415143966675 | 31.4667165279389 | 8.7840562179598 | 178.1833708286286 | 53.970485925674495 | 31.5415143966675 | 106.4667165279389 | |
8.79125626404009 | 28.179281949997 | 28.9659321308136 | 31.5308690071106 | 31.4107120037079 | 8.79125626404009 | 178.179281949997 | 53.9659321308136 | 31.5308690071106 | 106.4107120037079 | |
8.79845631012039 | 28.1751751899719 | 28.9575636386872 | 31.5165758132935 | 31.3562154769898 | 8.79845631012039 | 178.1751751899719 | 53.957563638687205 | 31.5165758132935 | 106.3562154769898 | |
8.80565635620068 | 28.1708419322968 | 28.9453506469727 | 31.4984321594238 | 31.3057959079743 | 8.80565635620068 | 178.1708419322968 | 53.9453506469727 | 31.4984321594238 | 106.3057959079743 | |
8.81285640228098 | 28.1660735607147 | 28.9298117160797 | 31.4769387245178 | 31.261557340622 | 8.81285640228098 | 178.1660735607147 | 53.9298117160797 | 31.4769387245178 | 106.261557340622 | |
8.82005644836127 | 28.1607210636139 | 28.9120256900788 | 31.4532220363617 | 31.225073337555 | 8.82005644836127 | 178.1607210636139 | 53.9120256900788 | 31.4532220363617 | 106.225073337555 | |
8.82725649444157 | 28.1548202037811 | 28.8935363292694 | 31.4287543296814 | 31.1971664428711 | 8.82725649444157 | 178.1548202037811 | 53.893536329269395 | 31.4287543296814 | 106.1971664428711 | |
8.83445654052186 | 28.1485199928284 | 28.8761377334595 | 31.4050972461701 | 31.1776280403137 | 8.83445654052186 | 178.1485199928284 | 53.8761377334595 | 31.4050972461701 | 106.17762804031369 | |
8.84165658660216 | 28.1420767307282 | 28.8613975048065 | 31.3837230205536 | 31.1652958393097 | 8.84165658660216 | 178.1420767307282 | 53.861397504806504 | 31.3837230205536 | 106.16529583930969 | |
8.84885663268245 | 28.1358361244202 | 28.8503527641297 | 31.3657879829407 | 31.1584055423737 | 8.84885663268245 | 178.1358361244202 | 53.850352764129696 | 31.3657879829407 | 106.1584055423737 | |
8.85605667876275 | 28.1301438808441 | 28.8433790206909 | 31.3518643379212 | 31.1552464962006 | 8.85605667876275 | 178.1301438808441 | 53.843379020690904 | 31.3518643379212 | 106.1552464962006 | |
8.86325672484304 | 28.1252861022949 | 28.8402557373047 | 31.3417732715607 | 31.1546683311463 | 8.86325672484304 | 178.1252861022949 | 53.8402557373047 | 31.3417732715607 | 106.1546683311463 | |
8.87045677092334 | 28.1215131282807 | 28.8403570652008 | 31.3348233699799 | 31.1562836170197 | 8.87045677092334 | 178.1215131282807 | 53.8403570652008 | 31.3348233699799 | 106.1562836170197 | |
8.87765681700363 | 28.1189858913422 | 28.8428485393524 | 31.3301503658295 | 31.1601758003235 | 8.87765681700363 | 178.1189858913422 | 53.8428485393524 | 31.3301503658295 | 106.1601758003235 | |
8.88485686308393 | 28.1177341938019 | 28.8467824459076 | 31.3271224498749 | 31.1667263507843 | 8.88485686308393 | 178.1177341938019 | 53.8467824459076 | 31.3271224498749 | 106.1667263507843 | |
8.89205690916422 | 28.1176745891571 | 28.8512051105499 | 31.3254296779633 | 31.1764478683472 | 8.89205690916422 | 178.1176745891571 | 53.8512051105499 | 31.3254296779633 | 106.1764478683472 | |
8.89925695524452 | 28.1185626983643 | 28.8552641868592 | 31.3248872756958 | 31.1899721622467 | 8.89925695524452 | 178.11856269836431 | 53.8552641868592 | 31.3248872756958 | 106.1899721622467 | |
8.90645700132481 | 28.1200885772705 | 28.8584351539612 | 31.3252687454224 | 31.2078833580017 | 8.90645700132481 | 178.1200885772705 | 53.858435153961196 | 31.3252687454224 | 106.2078833580017 | |
8.9136570474051 | 28.1220436096192 | 28.8605928421021 | 31.3262701034546 | 31.2305331230164 | 8.9136570474051 | 178.1220436096192 | 53.8605928421021 | 31.3262701034546 | 106.2305331230164 | |
8.9208570934854 | 28.124350309372 | 28.8620114326477 | 31.3275933265686 | 31.2577724456787 | 8.9208570934854 | 178.124350309372 | 53.862011432647705 | 31.3275933265686 | 106.2577724456787 | |
8.9280571395657 | 28.1270205974579 | 28.863126039505 | 31.3291013240814 | 31.2891244888306 | 8.9280571395657 | 178.12702059745789 | 53.863126039505005 | 31.3291013240814 | 106.2891244888306 | |
8.93525718564599 | 28.1300365924835 | 28.8643658161164 | 31.3307404518128 | 31.3239812850952 | 8.93525718564599 | 178.1300365924835 | 53.864365816116404 | 31.3307404518128 | 106.3239812850952 | |
8.94245723172629 | 28.1332075595856 | 28.8661003112793 | 31.3323974609375 | 31.3617706298828 | 8.94245723172629 | 178.1332075595856 | 53.8661003112793 | 31.3323974609375 | 106.3617706298828 | |
8.94965727780658 | 28.1362414360047 | 28.8686573505402 | 31.333863735199 | 31.4018845558167 | 8.94965727780658 | 178.1362414360047 | 53.868657350540204 | 31.333863735199 | 106.40188455581671 | |
8.95685732388687 | 28.1388938426972 | 28.8722574710846 | 31.3350915908814 | 31.4434051513672 | 8.95685732388687 | 178.1388938426972 | 53.872257471084595 | 31.3350915908814 | 106.4434051513672 | |
8.96405736996717 | 28.1411051750183 | 28.8769364356995 | 31.3363075256348 | 31.4849972724915 | 8.96405736996717 | 178.1411051750183 | 53.8769364356995 | 31.3363075256348 | 106.4849972724915 | |
8.97125741604746 | 28.1430244445801 | 28.8824439048767 | 31.3379645347595 | 31.5250039100647 | 8.97125741604746 | 178.1430244445801 | 53.882443904876695 | 31.3379645347595 | 106.5250039100647 | |
8.97845746212776 | 28.144907951355 | 28.8882911205292 | 31.3402175903321 | 31.5614938735962 | 8.97845746212776 | 178.144907951355 | 53.8882911205292 | 31.3402175903321 | 106.56149387359619 | |
8.98565750820806 | 28.1469821929932 | 28.8938760757446 | 31.3425004482269 | 31.592345237732 | 8.98565750820806 | 178.1469821929932 | 53.8938760757446 | 31.3425004482269 | 106.592345237732 | |
8.99285755428835 | 28.1493544578552 | 28.898572921753 | 31.343561410904 | 31.6153287887573 | 8.99285755428835 | 178.1493544578552 | 53.898572921753 | 31.343561410904 | 106.6153287887573 | |
9.00005760036864 | 28.152072429657 | 28.901880979538 | 31.3419103622437 | 31.6283762454987 | 9.00005760036864 | 178.152072429657 | 53.901880979538 | 31.3419103622437 | 106.6283762454987 | |
9.00725764644894 | 28.1550168991089 | 28.9035499095917 | 31.3362419605255 | 31.6298842430115 | 9.00725764644894 | 178.1550168991089 | 53.9035499095917 | 31.3362419605255 | 106.6298842430115 | |
9.01445769252923 | 28.1578660011292 | 28.9036512374878 | 31.3255190849304 | 31.6188752651215 | 9.01445769252923 | 178.1578660011292 | 53.9036512374878 | 31.3255190849304 | 106.6188752651215 | |
9.02165773860953 | 28.1601011753082 | 28.9025723934174 | 31.3089668750763 | 31.5949678421021 | 9.02165773860953 | 178.1601011753082 | 53.9025723934174 | 31.3089668750763 | 106.59496784210211 | |
9.02885778468983 | 28.1611800193787 | 28.9007365703583 | 31.2860608100891 | 31.5583467483521 | 9.02885778468983 | 178.1611800193787 | 53.900736570358305 | 31.2860608100891 | 106.55834674835211 | |
9.03605783077012 | 28.1606912612915 | 28.8984656333924 | 31.2568008899689 | 31.5098345279694 | 9.03605783077012 | 178.1606912612915 | 53.898465633392405 | 31.2568008899689 | 106.5098345279694 | |
9.04325787685041 | 28.1585037708283 | 28.895914554596 | 31.2217772006989 | 31.4511001110077 | 9.04325787685041 | 178.1585037708283 | 53.895914554596004 | 31.2217772006989 | 106.4511001110077 | |
9.05045792293071 | 28.1546652317047 | 28.8932144641876 | 31.182062625885 | 31.3846230506897 | 9.05045792293071 | 178.1546652317047 | 53.8932144641876 | 31.182062625885 | 106.3846230506897 | |
9.057657969011 | 28.1494915485382 | 28.8905560970307 | 31.138950586319 | 31.3134014606476 | 9.057657969011 | 178.1494915485382 | 53.8905560970307 | 31.138950586319 | 106.3134014606476 | |
9.0648580150913 | 28.1435966491699 | 28.8880586624146 | 31.0938358306885 | 31.2406480312348 | 9.0648580150913 | 178.1435966491699 | 53.8880586624146 | 31.0938358306885 | 106.2406480312348 | |
9.07205806117159 | 28.138017654419 | 28.8856327533722 | 31.0482740402222 | 31.1696112155915 | 9.07205806117159 | 178.138017654419 | 53.8856327533722 | 31.0482740402222 | 106.1696112155915 | |
9.07925810725189 | 28.1339108943939 | 28.8830697536469 | 31.0039520263672 | 31.1034142971039 | 9.07925810725189 | 178.1339108943939 | 53.8830697536469 | 31.0039520263672 | 106.1034142971039 | |
9.08645815333218 | 28.1321108341217 | 28.8802862167359 | 30.9627234935761 | 31.0447335243225 | 9.08645815333218 | 178.1321108341217 | 53.8802862167359 | 30.9627234935761 | 106.0447335243225 | |
9.09365819941248 | 28.1329393386841 | 28.8775384426117 | 30.9264242649079 | 30.9953331947327 | 9.09365819941248 | 178.1329393386841 | 53.8775384426117 | 30.9264242649079 | 105.9953331947327 | |
9.10085824549277 | 28.1363904476166 | 28.8751542568207 | 30.8966994285584 | 30.9556901454926 | 9.10085824549277 | 178.1363904476166 | 53.8751542568207 | 30.8966994285584 | 105.9556901454926 | |
9.10805829157307 | 28.142374753952 | 28.8731336593628 | 30.8747828006745 | 30.9250354766846 | 9.10805829157307 | 178.142374753952 | 53.8731336593628 | 30.8747828006745 | 105.9250354766846 | |
9.11525833765336 | 28.150749206543 | 28.8710296154022 | 30.8611750602722 | 30.9017717838288 | 9.11525833765336 | 178.150749206543 | 53.8710296154022 | 30.8611750602722 | 105.90177178382879 | |
9.12245838373366 | 28.1610786914826 | 28.8681387901306 | 30.8555364608765 | 30.8838605880738 | 9.12245838373366 | 178.1610786914826 | 53.8681387901306 | 30.8555364608765 | 105.8838605880738 | |
9.12965842981395 | 28.1724691390991 | 28.8637757301331 | 30.8568000793457 | 30.8690369129181 | 9.12965842981395 | 178.1724691390991 | 53.8637757301331 | 30.8568000793457 | 105.8690369129181 | |
9.13685847589425 | 28.1838774681092 | 28.8573622703552 | 30.8634161949158 | 30.8550357818604 | 9.13685847589425 | 178.1838774681092 | 53.857362270355196 | 30.8634161949158 | 105.85503578186041 | |
9.14405852197454 | 28.1945943832398 | 28.8483619689942 | 30.8735489845276 | 30.8399617671967 | 9.14405852197454 | 178.1945943832398 | 53.8483619689942 | 30.8735489845276 | 105.8399617671967 | |
9.15125856805484 | 28.2043695449829 | 28.8364052772522 | 30.8852732181549 | 30.8228611946106 | 9.15125856805484 | 178.2043695449829 | 53.8364052772522 | 30.8852732181549 | 105.8228611946106 | |
9.15845861413513 | 28.2131612300873 | 28.8216173648834 | 30.8966398239136 | 30.8040022850037 | 9.15845861413513 | 178.2131612300873 | 53.8216173648834 | 30.8966398239136 | 105.8040022850037 | |
9.16565866021543 | 28.2207131385803 | 28.8046717643738 | 30.9058606624603 | 30.7847380638123 | 9.16565866021543 | 178.2207131385803 | 53.8046717643738 | 30.9058606624603 | 105.7847380638123 | |
9.17285870629572 | 28.2264173030853 | 28.7866294384003 | 30.9116005897522 | 30.7669758796692 | 9.17285870629572 | 178.2264173030853 | 53.7866294384003 | 30.9116005897522 | 105.7669758796692 | |
9.18005875237602 | 28.2296240329743 | 28.768527507782 | 30.913120508194 | 30.7526111602783 | 9.18005875237602 | 178.2296240329743 | 53.768527507782 | 30.913120508194 | 105.75261116027829 | |
9.18725879845631 | 28.2300353050232 | 28.7513077259064 | 30.9103667736054 | 30.743157863617 | 9.18725879845631 | 178.2300353050232 | 53.7513077259064 | 30.9103667736054 | 105.743157863617 | |
9.19445884453661 | 28.2277166843415 | 28.7359595298767 | 30.9038937091828 | 30.7394862174988 | 9.19445884453661 | 178.2277166843415 | 53.735959529876695 | 30.9038937091828 | 105.73948621749881 | |
9.2016588906169 | 28.2230019569397 | 28.7234723567963 | 30.8947503566742 | 30.7416379451752 | 9.2016588906169 | 178.2230019569397 | 53.7234723567963 | 30.8947503566742 | 105.7416379451752 | |
9.2088589366972 | 28.2163381576538 | 28.7144601345062 | 30.884337425232 | 30.7486534118653 | 9.2088589366972 | 178.2163381576538 | 53.7144601345062 | 30.884337425232 | 105.74865341186529 | |
9.21605898277749 | 28.2083451747894 | 28.7087321281433 | 30.8740794658661 | 30.7586193084717 | 9.21605898277749 | 178.2083451747894 | 53.708732128143296 | 30.8740794658661 | 105.75861930847171 | |
9.22325902885779 | 28.1999230384827 | 28.705233335495 | 30.8650314807892 | 30.7690799236298 | 9.22325902885779 | 178.1999230384827 | 53.705233335494995 | 30.8650314807892 | 105.7690799236298 | |
9.23045907493808 | 28.1920909881592 | 28.7025570869446 | 30.857527256012 | 30.7775437831879 | 9.23045907493808 | 178.1920909881592 | 53.7025570869446 | 30.857527256012 | 105.7775437831879 | |
9.23765912101838 | 28.1855404376984 | 28.6994516849518 | 30.8511078357697 | 30.7819724082947 | 9.23765912101838 | 178.1855404376984 | 53.699451684951796 | 30.8511078357697 | 105.7819724082947 | |
9.24485916709867 | 28.1802475452423 | 28.6948800086975 | 30.8446228504181 | 30.781090259552 | 9.24485916709867 | 178.1802475452423 | 53.694880008697496 | 30.8446228504181 | 105.781090259552 | |
9.25205921317897 | 28.1754851341248 | 28.6880791187287 | 30.8364987373352 | 30.7743608951569 | 9.25205921317897 | 178.1754851341248 | 53.6880791187287 | 30.8364987373352 | 105.7743608951569 | |
9.25925925925926 | 28.1700909137726 | 28.6786377429962 | 30.8249473571778 | 30.7617962360382 | 9.25925925925926 | 178.1700909137726 | 53.6786377429962 | 30.8249473571778 | 105.76179623603821 | |
9.26645930533956 | 28.1628727912903 | 28.6667704582215 | 30.8083534240723 | 30.7436883449555 | 9.26645930533956 | 178.1628727912903 | 53.6667704582215 | 30.8083534240723 | 105.7436883449555 | |
9.27365935141985 | 28.1529247760773 | 28.6535084247589 | 30.7855665683747 | 30.7204961776734 | 9.27365935141985 | 178.1529247760773 | 53.6535084247589 | 30.7855665683747 | 105.7204961776734 | |
9.28085939750015 | 28.1397819519043 | 28.6403954029083 | 30.7562708854676 | 30.6928753852844 | 9.28085939750015 | 178.1397819519043 | 53.6403954029083 | 30.7562708854676 | 105.6928753852844 | |
9.28805944358044 | 28.1235277652741 | 28.6288559436798 | 30.7209670543671 | 30.661678314209 | 9.28805944358044 | 178.1235277652741 | 53.628855943679795 | 30.7209670543671 | 105.661678314209 | |
9.29525948966074 | 28.1049609184265 | 28.6197245121002 | 30.6806921958924 | 30.6279182434082 | 9.29525948966074 | 178.1049609184265 | 53.6197245121002 | 30.6806921958924 | 105.6279182434082 | |
9.30245953574103 | 28.0854105949402 | 28.6130785942078 | 30.6366801261902 | 30.5925607681275 | 9.30245953574103 | 178.08541059494019 | 53.6130785942078 | 30.6366801261902 | 105.5925607681275 | |
9.30965958182133 | 28.0664443969727 | 28.6084592342377 | 30.5902123451233 | 30.5564403533936 | 9.30965958182133 | 178.0664443969727 | 53.6084592342377 | 30.5902123451233 | 105.5564403533936 | |
9.31685962790162 | 28.0494332313538 | 28.6051332950592 | 30.5426895618439 | 30.5203258991242 | 9.31685962790162 | 178.0494332313538 | 53.605133295059204 | 30.5426895618439 | 105.5203258991242 | |
9.32405967398192 | 28.0351161956787 | 28.6022424697876 | 30.4956316947937 | 30.4850101470947 | 9.32405967398192 | 178.0351161956787 | 53.6022424697876 | 30.4956316947937 | 105.4850101470947 | |
9.33125972006221 | 28.0236601829529 | 28.5989701747894 | 30.4506301879883 | 30.4512441158295 | 9.33125972006221 | 178.0236601829529 | 53.5989701747894 | 30.4506301879883 | 105.4512441158295 | |
9.3384597661425 | 28.0148267745972 | 28.5947859287262 | 30.4091572761536 | 30.4195284843445 | 9.3384597661425 | 178.0148267745972 | 53.594785928726196 | 30.4091572761536 | 105.4195284843445 |