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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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7.90565059616382 | 28.6595761775971 | 29.4061124324799 | 33.0828368663788 | 32.5277388095856 | 7.90565059616382 | 178.6595761775971 | 54.4061124324799 | 33.0828368663788 | 107.5277388095856 | |
7.91285064224411 | 28.670072555542 | 29.4148802757263 | 33.1189095973969 | 32.5209617614746 | 7.91285064224411 | 178.670072555542 | 54.414880275726304 | 33.1189095973969 | 107.52096176147461 | |
7.92005068832441 | 28.6785960197449 | 29.4262826442719 | 33.1403493881226 | 32.503867149353 | 7.92005068832441 | 178.6785960197449 | 54.4262826442719 | 33.1403493881226 | 107.503867149353 | |
7.9272507344047 | 28.6847054958344 | 29.4396221637726 | 33.1446349620819 | 32.4759542942047 | 7.9272507344047 | 178.6847054958344 | 54.4396221637726 | 33.1446349620819 | 107.4759542942047 | |
7.934450780485 | 28.687995672226 | 29.4537544250488 | 33.1299662590027 | 32.4369311332703 | 7.934450780485 | 178.687995672226 | 54.4537544250488 | 33.1299662590027 | 107.43693113327029 | |
7.94165082656529 | 28.6882102489472 | 29.4673144817353 | 33.0951929092407 | 32.3867022991181 | 7.94165082656529 | 178.6882102489472 | 54.4673144817353 | 33.0951929092407 | 107.3867022991181 | |
7.94885087264559 | 28.685337305069 | 29.4789254665375 | 33.0397367477417 | 32.3256433010102 | 7.94885087264559 | 178.685337305069 | 54.478925466537504 | 33.0397367477417 | 107.3256433010102 | |
7.95605091872588 | 28.679621219635 | 29.4873535633087 | 32.9639613628388 | 32.2547912597656 | 7.95605091872588 | 178.679621219635 | 54.4873535633087 | 32.9639613628388 | 107.2547912597656 | |
7.96325096480618 | 28.6715924739838 | 29.4915795326233 | 32.8696787357331 | 32.1759521961212 | 7.96325096480618 | 178.6715924739838 | 54.491579532623305 | 32.8696787357331 | 107.1759521961212 | |
7.97045101088647 | 28.6619007587433 | 29.4909179210663 | 32.7602446079254 | 32.0915937423706 | 7.97045101088647 | 178.6619007587433 | 54.4909179210663 | 32.7602446079254 | 107.0915937423706 | |
7.97765105696677 | 28.651225566864 | 29.4853448867798 | 32.6400935649872 | 32.0045948028565 | 7.97765105696677 | 178.651225566864 | 54.4853448867798 | 32.6400935649872 | 107.0045948028565 | |
7.98485110304706 | 28.6403298377991 | 29.4755280017853 | 32.5141072273255 | 31.9178938865662 | 7.98485110304706 | 178.6403298377991 | 54.4755280017853 | 32.5141072273255 | 106.9178938865662 | |
7.99205114912736 | 28.6301374435425 | 29.4627249240875 | 32.3870956897736 | 31.8341910839081 | 7.99205114912736 | 178.6301374435425 | 54.462724924087496 | 32.3870956897736 | 106.8341910839081 | |
7.99925119520765 | 28.6217272281647 | 29.4484198093415 | 32.2635471820832 | 31.7556917667389 | 7.99925119520765 | 178.6217272281647 | 54.4484198093415 | 32.2635471820832 | 106.75569176673889 | |
8.00645124128795 | 28.6161601543427 | 29.4339716434479 | 32.1474015712738 | 31.6841065883637 | 8.00645124128795 | 178.6161601543427 | 54.433971643447904 | 32.1474015712738 | 106.6841065883637 | |
8.01365128736824 | 28.6141574382782 | 29.4204771518707 | 32.0416152477265 | 31.6205501556397 | 8.01365128736824 | 178.6141574382782 | 54.4204771518707 | 32.0416152477265 | 106.6205501556397 | |
8.02085133344854 | 28.6158800125122 | 29.408746957779 | 31.9477319717407 | 31.5653502941132 | 8.02085133344854 | 178.6158800125122 | 54.408746957779 | 31.9477319717407 | 106.5653502941132 | |
8.02805137952883 | 28.6211311817169 | 29.3991088867188 | 31.8659245967865 | 31.5179347991944 | 8.02805137952883 | 178.6211311817169 | 54.3991088867188 | 31.8659245967865 | 106.51793479919439 | |
8.03525142560913 | 28.6296248435974 | 29.3913066387177 | 31.7952692508698 | 31.4768493175507 | 8.03525142560913 | 178.6296248435974 | 54.3913066387177 | 31.7952692508698 | 106.4768493175507 | |
8.04245147168942 | 28.6411583423615 | 29.3844759464264 | 31.7343652248383 | 31.4402043819428 | 8.04245147168942 | 178.6411583423615 | 54.3844759464264 | 31.7343652248383 | 106.4402043819428 | |
8.04965151776972 | 28.6554336547852 | 29.3774545192719 | 31.6815853118897 | 31.4062237739563 | 8.04965151776972 | 178.6554336547852 | 54.3774545192719 | 31.6815853118897 | 106.4062237739563 | |
8.05685156385001 | 28.6717116832733 | 29.3691575527191 | 31.635183095932 | 31.3735008239746 | 8.05685156385001 | 178.67171168327332 | 54.3691575527191 | 31.635183095932 | 106.3735008239746 | |
8.0640516099303 | 28.6887228488922 | 29.358845949173 | 31.5933525562287 | 31.3409388065338 | 8.0640516099303 | 178.6887228488922 | 54.358845949173 | 31.5933525562287 | 106.3409388065338 | |
8.0712516560106 | 28.7050426006317 | 29.3462693691254 | 31.5544247627259 | 31.3076734542847 | 8.0712516560106 | 178.7050426006317 | 54.3462693691254 | 31.5544247627259 | 106.3076734542847 | |
8.0784517020909 | 28.7195324897766 | 29.3317317962647 | 31.5171003341675 | 31.273227930069 | 8.0784517020909 | 178.7195324897766 | 54.331731796264705 | 31.5171003341675 | 106.273227930069 | |
8.08565174817119 | 28.7314355373383 | 29.3162882328034 | 31.48073554039 | 31.2378466129303 | 8.08565174817119 | 178.7314355373383 | 54.3162882328034 | 31.48073554039 | 106.2378466129303 | |
8.09285179425149 | 28.7401497364044 | 29.3016612529755 | 31.445175409317 | 31.2026977539063 | 8.09285179425149 | 178.7401497364044 | 54.3016612529755 | 31.445175409317 | 106.2026977539063 | |
8.10005184033178 | 28.7449955940247 | 29.2900979518891 | 31.4106702804566 | 31.1696231365204 | 8.10005184033178 | 178.74499559402472 | 54.290097951889095 | 31.4106702804566 | 106.1696231365204 | |
8.10725188641207 | 28.7453770637512 | 29.2840540409088 | 31.3777685165405 | 31.1405301094055 | 8.10725188641207 | 178.7453770637512 | 54.2840540409088 | 31.3777685165405 | 106.1405301094055 | |
8.11445193249237 | 28.7413060665131 | 29.2856991291046 | 31.3473343849182 | 31.1169087886811 | 8.11445193249237 | 178.7413060665131 | 54.2856991291046 | 31.3473343849182 | 106.1169087886811 | |
8.12165197857266 | 28.733491897583 | 29.2965352535248 | 31.320458650589 | 31.0997247695923 | 8.12165197857266 | 178.733491897583 | 54.2965352535248 | 31.320458650589 | 106.0997247695923 | |
8.12885202465296 | 28.7230908870697 | 29.3171405792237 | 31.2981426715851 | 31.0894072055817 | 8.12885202465296 | 178.7230908870697 | 54.317140579223704 | 31.2981426715851 | 106.0894072055817 | |
8.13605207073326 | 28.7112653255463 | 29.3469667434693 | 31.2808871269226 | 31.0857772827149 | 8.13605207073326 | 178.7112653255463 | 54.346966743469295 | 31.2808871269226 | 106.0857772827149 | |
8.14325211681355 | 28.6988496780396 | 29.3844163417816 | 31.2684416770935 | 31.0878336429596 | 8.14325211681355 | 178.6988496780396 | 54.3844163417816 | 31.2684416770935 | 106.0878336429596 | |
8.15045216289384 | 28.6863088607788 | 29.427057504654 | 31.2599360942841 | 31.0938239097595 | 8.15045216289384 | 178.6863088607788 | 54.427057504654 | 31.2599360942841 | 106.0938239097595 | |
8.15765220897414 | 28.673666715622 | 29.4718742370606 | 31.2541902065277 | 31.1016619205475 | 8.15765220897414 | 178.673666715622 | 54.471874237060604 | 31.2541902065277 | 106.1016619205475 | |
8.16485225505443 | 28.6603033542633 | 29.5156955718994 | 31.2499403953552 | 31.1094999313355 | 8.16485225505443 | 178.6603033542633 | 54.5156955718994 | 31.2499403953552 | 106.1094999313355 | |
8.17205230113473 | 28.6449134349823 | 29.5556962490082 | 31.2459468841553 | 31.1160862445832 | 8.17205230113473 | 178.6449134349823 | 54.5556962490082 | 31.2459468841553 | 106.1160862445832 | |
8.17925234721503 | 28.625762462616 | 29.5897305011749 | 31.240975856781 | 31.1207115650177 | 8.17925234721503 | 178.625762462616 | 54.5897305011749 | 31.240975856781 | 106.1207115650177 | |
8.18645239329532 | 28.6012053489685 | 29.6165406703949 | 31.2337875366211 | 31.1230540275574 | 8.18645239329532 | 178.6012053489685 | 54.6165406703949 | 31.2337875366211 | 106.1230540275574 | |
8.19365243937561 | 28.5701930522919 | 29.6357035636902 | 31.2231957912445 | 31.1231017112732 | 8.19365243937561 | 178.5701930522919 | 54.6357035636902 | 31.2231957912445 | 106.1231017112732 | |
8.20085248545591 | 28.5327076911926 | 29.6473503112793 | 31.208211183548 | 31.1210215091706 | 8.20085248545591 | 178.5327076911926 | 54.6473503112793 | 31.208211183548 | 106.1210215091706 | |
8.2080525315362 | 28.4899473190308 | 29.6518325805664 | 31.18816614151 | 31.1170041561127 | 8.2080525315362 | 178.4899473190308 | 54.6518325805664 | 31.18816614151 | 106.1170041561127 | |
8.2152525776165 | 28.4442305564881 | 29.6494007110596 | 31.1627388000488 | 31.1109066009522 | 8.2152525776165 | 178.4442305564881 | 54.6494007110596 | 31.1627388000488 | 106.1109066009522 | |
8.22245262369679 | 28.3986210823059 | 29.6400845050812 | 31.1318695545197 | 31.10191822052 | 8.22245262369679 | 178.3986210823059 | 54.640084505081205 | 31.1318695545197 | 106.10191822052 | |
8.22965266977709 | 28.3563375473023 | 29.6237051486969 | 31.0957610607147 | 31.0887515544892 | 8.22965266977709 | 178.3563375473023 | 54.6237051486969 | 31.0957610607147 | 106.08875155448919 | |
8.23685271585738 | 28.3200204372406 | 29.6000182628632 | 31.0549199581146 | 31.0699701309204 | 8.23685271585738 | 178.3200204372406 | 54.6000182628632 | 31.0549199581146 | 106.0699701309204 | |
8.24405276193768 | 28.2912611961365 | 29.5688033103943 | 31.0103178024292 | 31.0445845127106 | 8.24405276193768 | 178.2912611961365 | 54.5688033103943 | 31.0103178024292 | 106.0445845127106 | |
8.25125280801797 | 28.2704532146454 | 29.5299649238587 | 30.9635221958161 | 31.0124278068543 | 8.25125280801797 | 178.27045321464539 | 54.5299649238587 | 30.9635221958161 | 106.0124278068543 | |
8.25845285409827 | 28.2568275928498 | 29.4838130474091 | 30.9163928031922 | 30.9743463993073 | 8.25845285409827 | 178.2568275928498 | 54.4838130474091 | 30.9163928031922 | 105.97434639930731 | |
8.26565290017856 | 28.2487571239472 | 29.4313311576843 | 30.8705568313599 | 30.931967496872 | 8.26565290017856 | 178.2487571239472 | 54.4313311576843 | 30.8705568313599 | 105.931967496872 | |
8.27285294625886 | 28.2440781593323 | 29.3742775917053 | 30.8271169662476 | 30.8874547481537 | 8.27285294625886 | 178.2440781593323 | 54.3742775917053 | 30.8271169662476 | 105.8874547481537 | |
8.28005299233915 | 28.2404363155365 | 29.3149590492249 | 30.7865500450134 | 30.8431088924408 | 8.28005299233915 | 178.2404363155365 | 54.314959049224896 | 30.7865500450134 | 105.8431088924408 | |
8.28725303841945 | 28.2358229160309 | 29.2558372020722 | 30.7490110397339 | 30.8012366294861 | 8.28725303841945 | 178.2358229160309 | 54.2558372020722 | 30.7490110397339 | 105.8012366294861 | |
8.29445308449974 | 28.2289564609528 | 29.1992068290711 | 30.7145476341248 | 30.7638347148895 | 8.29445308449974 | 178.22895646095282 | 54.1992068290711 | 30.7145476341248 | 105.7638347148895 | |
8.30165313058004 | 28.2195210456848 | 29.1470229625702 | 30.6831181049347 | 30.7322919368744 | 8.30165313058004 | 178.21952104568481 | 54.147022962570205 | 30.6831181049347 | 105.7322919368744 | |
8.30885317666033 | 28.2080531120301 | 29.1008412837982 | 30.6545376777649 | 30.7069957256317 | 8.30885317666033 | 178.2080531120301 | 54.100841283798204 | 30.6545376777649 | 105.7069957256317 | |
8.31605322274063 | 28.1956017017365 | 29.0616154670716 | 30.6286156177521 | 30.6874215602875 | 8.31605322274063 | 178.1956017017365 | 54.061615467071604 | 30.6286156177521 | 105.6874215602875 | |
8.32325326882092 | 28.1833827495575 | 29.0295779705048 | 30.6053578853607 | 30.672687292099 | 8.32325326882092 | 178.1833827495575 | 54.0295779705048 | 30.6053578853607 | 105.672687292099 | |
8.33045331490122 | 28.1726717948914 | 29.0042340755463 | 30.5851817131043 | 30.662339925766 | 8.33045331490122 | 178.17267179489141 | 54.0042340755463 | 30.5851817131043 | 105.66233992576599 | |
8.33765336098151 | 28.1646370887757 | 28.984534740448 | 30.568927526474 | 30.656760931015 | 8.33765336098151 | 178.1646370887757 | 53.984534740448 | 30.568927526474 | 105.656760931015 | |
8.34485340706181 | 28.16002368927 | 28.9692282676697 | 30.5576026439667 | 30.6569755077362 | 8.34485340706181 | 178.16002368927 | 53.9692282676697 | 30.5576026439667 | 105.6569755077362 | |
8.3520534531421 | 28.1588912010193 | 28.957200050354 | 30.5520713329315 | 30.663937330246 | 8.3520534531421 | 178.1588912010193 | 53.957200050354004 | 30.5520713329315 | 105.663937330246 | |
8.3592534992224 | 28.1605243682862 | 28.9475977420807 | 30.5529236793518 | 30.6781232357025 | 8.3592534992224 | 178.1605243682862 | 53.9475977420807 | 30.5529236793518 | 105.6781232357025 | |
8.36645354530269 | 28.1636655330658 | 28.9398193359375 | 30.5604875087738 | 30.6993961334229 | 8.36645354530269 | 178.1636655330658 | 53.9398193359375 | 30.5604875087738 | 105.69939613342291 | |
8.37365359138299 | 28.1668722629547 | 28.9334118366242 | 30.5748999118805 | 30.7271301746369 | 8.37365359138299 | 178.1668722629547 | 53.9334118366242 | 30.5748999118805 | 105.7271301746369 | |
8.38085363746328 | 28.1688332557678 | 28.9281010627747 | 30.5960237979889 | 30.7602226734162 | 8.38085363746328 | 178.1688332557678 | 53.9281010627747 | 30.5960237979889 | 105.7602226734162 | |
8.38805368354358 | 28.1686007976532 | 28.9238810539246 | 30.6233048439026 | 30.7967901229859 | 8.38805368354358 | 178.1686007976532 | 53.9238810539246 | 30.6233048439026 | 105.7967901229859 | |
8.39525372962387 | 28.1659245491028 | 28.9210677146912 | 30.6556105613709 | 30.8341026306153 | 8.39525372962387 | 178.1659245491028 | 53.921067714691205 | 30.6556105613709 | 105.83410263061529 | |
8.40245377570417 | 28.161484003067 | 28.9201140403748 | 30.6913912296295 | 30.8689892292023 | 8.40245377570417 | 178.161484003067 | 53.9201140403748 | 30.6913912296295 | 105.8689892292023 | |
8.40965382178446 | 28.1569242477417 | 28.9212942123413 | 30.7289898395539 | 30.898779630661 | 8.40965382178446 | 178.1569242477417 | 53.9212942123413 | 30.7289898395539 | 105.898779630661 | |
8.41685386786476 | 28.1545460224152 | 28.9245665073395 | 30.7670295238495 | 30.9220910072327 | 8.41685386786476 | 178.1545460224152 | 53.9245665073395 | 30.7670295238495 | 105.9220910072327 | |
8.42405391394505 | 28.1565427780152 | 28.9296686649323 | 30.8044910430908 | 30.9390008449555 | 8.42405391394505 | 178.1565427780152 | 53.9296686649323 | 30.8044910430908 | 105.9390008449555 | |
8.43125396002535 | 28.1643152236939 | 28.9362370967865 | 30.8407068252564 | 30.9503734111786 | 8.43125396002535 | 178.1643152236939 | 53.9362370967865 | 30.8407068252564 | 105.9503734111786 | |
8.43845400610564 | 28.1781077384949 | 28.9439260959625 | 30.8751881122589 | 30.9570729732514 | 8.43845400610564 | 178.1781077384949 | 53.943926095962496 | 30.8751881122589 | 105.9570729732514 | |
8.44565405218594 | 28.1969964504242 | 28.9523899555207 | 30.9076845645905 | 30.9596359729767 | 8.44565405218594 | 178.1969964504242 | 53.9523899555207 | 30.9076845645905 | 105.9596359729767 | |
8.45285409826623 | 28.2191753387451 | 28.9611339569092 | 30.9383392333985 | 30.958491563797 | 8.45285409826623 | 178.2191753387451 | 53.9611339569092 | 30.9383392333985 | 105.958491563797 | |
8.46005414434653 | 28.2424449920655 | 28.9696574211121 | 30.9675514698029 | 30.9541583061218 | 8.46005414434653 | 178.2424449920655 | 53.9696574211121 | 30.9675514698029 | 105.9541583061218 | |
8.46725419042682 | 28.2646179199219 | 28.9775609970093 | 30.9956848621369 | 30.9471607208252 | 8.46725419042682 | 178.2646179199219 | 53.9775609970093 | 30.9956848621369 | 105.9471607208252 | |
8.47445423650712 | 28.28409075737 | 28.984659910202 | 31.0227572917938 | 30.9377133846283 | 8.47445423650712 | 178.28409075737 | 53.984659910202 | 31.0227572917938 | 105.9377133846283 | |
8.48165428258741 | 28.3002436161041 | 28.9909362792969 | 31.0483098030091 | 30.9256792068482 | 8.48165428258741 | 178.3002436161041 | 53.9909362792969 | 31.0483098030091 | 105.9256792068482 | |
8.48885432866771 | 28.3137381076813 | 28.9965212345124 | 31.0716629028321 | 30.9110939502716 | 8.48885432866771 | 178.3137381076813 | 53.9965212345124 | 31.0716629028321 | 105.9110939502716 | |
8.496054374748 | 28.3263146877289 | 29.0015459060669 | 31.092232465744 | 30.8946549892426 | 8.496054374748 | 178.3263146877289 | 54.0015459060669 | 31.092232465744 | 105.8946549892426 | |
8.5032544208283 | 28.3402383327484 | 29.0061056613922 | 31.1095595359802 | 30.8778464794159 | 8.5032544208283 | 178.3402383327484 | 54.0061056613922 | 31.1095595359802 | 105.8778464794159 | |
8.51045446690859 | 28.3574640750885 | 29.010421037674 | 31.1232209205628 | 30.8623731136322 | 8.51045446690859 | 178.3574640750885 | 54.010421037674 | 31.1232209205628 | 105.8623731136322 | |
8.51765451298889 | 28.3791363239289 | 29.0148854255676 | 31.1328649520874 | 30.8495819568634 | 8.51765451298889 | 178.3791363239289 | 54.0148854255676 | 31.1328649520874 | 105.8495819568634 | |
8.52485455906918 | 28.4055054187775 | 29.019969701767 | 31.1384201049805 | 30.8402240276337 | 8.52485455906918 | 178.4055054187775 | 54.019969701766996 | 31.1384201049805 | 105.8402240276337 | |
8.53205460514948 | 28.4360587596894 | 29.0261745452881 | 31.140285730362 | 30.8346867561341 | 8.53205460514948 | 178.4360587596894 | 54.0261745452881 | 31.140285730362 | 105.8346867561341 | |
8.53925465122977 | 28.4696400165558 | 29.0336906909943 | 31.1393737792969 | 30.8332443237305 | 8.53925465122977 | 178.4696400165558 | 54.033690690994305 | 31.1393737792969 | 105.8332443237305 | |
8.54645469731007 | 28.5045623779297 | 29.0422797203064 | 31.1367750167847 | 30.836021900177 | 8.54645469731007 | 178.5045623779297 | 54.0422797203064 | 31.1367750167847 | 105.836021900177 | |
8.55365474339036 | 28.5386383533478 | 29.0514588356018 | 31.1335265636444 | 30.8429956436157 | 8.55365474339036 | 178.5386383533478 | 54.0514588356018 | 31.1335265636444 | 105.8429956436157 | |
8.56085478947066 | 28.5697460174561 | 29.0606856346131 | 31.1304748058319 | 30.854070186615 | 8.56085478947066 | 178.5697460174561 | 54.0606856346131 | 31.1304748058319 | 105.854070186615 | |
8.56805483555095 | 28.5962283611298 | 29.0695786476135 | 31.1282396316529 | 30.8693945407868 | 8.56805483555095 | 178.5962283611298 | 54.0695786476135 | 31.1282396316529 | 105.8693945407868 | |
8.57525488163125 | 28.6170244216919 | 29.0779829025269 | 31.1272084712982 | 30.8895170688629 | 8.57525488163125 | 178.6170244216919 | 54.0779829025269 | 31.1272084712982 | 105.8895170688629 | |
8.58245492771154 | 28.6315143108368 | 29.0858864784241 | 31.1275362968445 | 30.9150636196137 | 8.58245492771154 | 178.6315143108368 | 54.0858864784241 | 31.1275362968445 | 105.9150636196137 | |
8.58965497379184 | 28.6392509937287 | 29.0934801101685 | 31.12912774086 | 30.9463739395142 | 8.58965497379184 | 178.6392509937287 | 54.0934801101685 | 31.12912774086 | 105.9463739395142 | |
8.59685501987213 | 28.6398649215698 | 29.1012287139893 | 31.1317801475525 | 30.9831917285919 | 8.59685501987213 | 178.6398649215698 | 54.1012287139893 | 31.1317801475525 | 105.9831917285919 | |
8.60405506595242 | 28.6332905292511 | 29.1098415851593 | 31.1353981494904 | 31.0248374938965 | 8.60405506595242 | 178.6332905292511 | 54.1098415851593 | 31.1353981494904 | 106.0248374938965 | |
8.61125511203272 | 28.6198914051056 | 29.1198670864105 | 31.1401784420014 | 31.07048869133 | 8.61125511203272 | 178.6198914051056 | 54.1198670864105 | 31.1401784420014 | 106.07048869133 | |
8.61845515811302 | 28.6003768444061 | 29.1313350200653 | 31.1467289924622 | 31.11931681633 | 8.61845515811302 | 178.6003768444061 | 54.1313350200653 | 31.1467289924622 | 106.11931681633 |