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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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7.18564598813433 | 28.5894572734833 | 29.6100914478302 | 30.8060765266419 | 30.5943429470062 | 7.18564598813433 | 178.5894572734833 | 54.6100914478302 | 30.8060765266419 | 105.5943429470062 | |
7.19284603421462 | 28.5772025585175 | 29.5441806316376 | 30.7905495166779 | 30.5835843086243 | 7.19284603421462 | 178.5772025585175 | 54.5441806316376 | 30.7905495166779 | 105.5835843086243 | |
7.20004608029492 | 28.5636305809021 | 29.490202665329 | 30.7765901088715 | 30.5726051330567 | 7.20004608029492 | 178.5636305809021 | 54.490202665329 | 30.7765901088715 | 105.5726051330567 | |
7.20724612637521 | 28.5490691661835 | 29.4479548931122 | 30.7657599449158 | 30.561625957489 | 7.20724612637521 | 178.5490691661835 | 54.4479548931122 | 30.7657599449158 | 105.561625957489 | |
7.21444617245551 | 28.5341858863831 | 29.4163823127747 | 30.7591438293457 | 30.551165342331 | 7.21444617245551 | 178.5341858863831 | 54.4163823127747 | 30.7591438293457 | 105.551165342331 | |
7.2216462185358 | 28.5198271274567 | 29.3940365314484 | 30.7572364807129 | 30.5419623851776 | 7.2216462185358 | 178.5198271274567 | 54.3940365314484 | 30.7572364807129 | 105.5419623851776 | |
7.2288462646161 | 28.5066723823548 | 29.3792366981507 | 30.7598650455475 | 30.53457736969 | 7.2288462646161 | 178.5066723823548 | 54.3792366981507 | 30.7598650455475 | 105.53457736969 | |
7.23604631069639 | 28.4950911998749 | 29.3699622154236 | 30.7662606239319 | 30.529248714447 | 7.23604631069639 | 178.4950911998749 | 54.3699622154236 | 30.7662606239319 | 105.529248714447 | |
7.24324635677669 | 28.4850776195526 | 29.3639659881592 | 30.7752192020416 | 30.5262088775635 | 7.24324635677669 | 178.4850776195526 | 54.3639659881592 | 30.7752192020416 | 105.5262088775635 | |
7.25044640285698 | 28.4763932228089 | 29.3593466281891 | 30.7853698730469 | 30.5257976055145 | 7.25044640285698 | 178.4763932228089 | 54.3593466281891 | 30.7853698730469 | 105.5257976055145 | |
7.25764644893728 | 28.4687697887421 | 29.3548583984375 | 30.7954609394074 | 30.528211593628 | 7.25764644893728 | 178.4687697887421 | 54.3548583984375 | 30.7954609394074 | 105.528211593628 | |
7.26484649501757 | 28.4621059894562 | 29.349833726883 | 30.8044612407685 | 30.53297996521 | 7.26484649501757 | 178.4621059894562 | 54.349833726883 | 30.8044612407685 | 105.53297996521 | |
7.27204654109787 | 28.4566938877106 | 29.3438851833344 | 30.8115541934967 | 30.5389702320099 | 7.27204654109787 | 178.4566938877106 | 54.3438851833344 | 30.8115541934967 | 105.5389702320099 | |
7.27924658717816 | 28.4531533718109 | 29.3367445468903 | 30.8160543441773 | 30.5448651313782 | 7.27924658717816 | 178.4531533718109 | 54.3367445468903 | 30.8160543441773 | 105.5448651313782 | |
7.28644663325845 | 28.4522712230683 | 29.3281674385071 | 30.8175802230835 | 30.5497705936432 | 7.28644663325845 | 178.4522712230683 | 54.3281674385071 | 30.8175802230835 | 105.5497705936432 | |
7.29364667933875 | 28.45458984375 | 29.3180048465729 | 30.816400051117 | 30.5534660816193 | 7.29364667933875 | 178.45458984375 | 54.318004846572904 | 30.816400051117 | 105.5534660816193 | |
7.30084672541905 | 28.460156917572 | 29.3061912059784 | 30.813479423523 | 30.5561184883118 | 7.30084672541905 | 178.460156917572 | 54.3061912059784 | 30.813479423523 | 105.5561184883118 | |
7.30804677149934 | 28.4684777259827 | 29.2927980422974 | 30.8103680610657 | 30.5581390857697 | 7.30804677149934 | 178.4684777259827 | 54.2927980422974 | 30.8103680610657 | 105.5581390857697 | |
7.31524681757964 | 28.4786283969879 | 29.2781114578247 | 30.8087348937988 | 30.5602431297302 | 7.31524681757964 | 178.47862839698792 | 54.2781114578247 | 30.8087348937988 | 105.5602431297302 | |
7.32244686365993 | 28.4894049167633 | 29.2627215385437 | 30.8100461959839 | 30.5635869503021 | 7.32244686365993 | 178.4894049167633 | 54.2627215385437 | 30.8100461959839 | 105.5635869503021 | |
7.32964690974022 | 28.4995198249817 | 29.2473077774048 | 30.8154344558716 | 30.5694222450257 | 7.32964690974022 | 178.4995198249817 | 54.2473077774048 | 30.8154344558716 | 105.56942224502569 | |
7.33684695582052 | 28.5079121589661 | 29.2326152324677 | 30.8256030082703 | 30.5786728858948 | 7.33684695582052 | 178.5079121589661 | 54.2326152324677 | 30.8256030082703 | 105.5786728858948 | |
7.34404700190081 | 28.514051437378 | 29.2195737361908 | 30.8404922485352 | 30.5914878845215 | 7.34404700190081 | 178.514051437378 | 54.219573736190796 | 30.8404922485352 | 105.5914878845215 | |
7.35124704798111 | 28.5182118415833 | 29.2093932628632 | 30.8592319488526 | 30.6073665618897 | 7.35124704798111 | 178.5182118415833 | 54.2093932628632 | 30.8592319488526 | 105.6073665618897 | |
7.35844709406141 | 28.521466255188 | 29.2034387588501 | 30.8802962303162 | 30.6255578994751 | 7.35844709406141 | 178.521466255188 | 54.2034387588501 | 30.8802962303162 | 105.6255578994751 | |
7.3656471401417 | 28.5252153873444 | 29.2027235031128 | 30.9019684791565 | 30.6454002857208 | 7.3656471401417 | 178.5252153873444 | 54.2027235031128 | 30.9019684791565 | 105.6454002857208 | |
7.37284718622199 | 28.5306453704834 | 29.2074263095856 | 30.9229969978333 | 30.6664705276489 | 7.37284718622199 | 178.5306453704834 | 54.2074263095856 | 30.9229969978333 | 105.6664705276489 | |
7.38004723230229 | 28.5382807254791 | 29.2169570922852 | 30.9429347515106 | 30.6885659694672 | 7.38004723230229 | 178.5382807254791 | 54.2169570922852 | 30.9429347515106 | 105.68856596946719 | |
7.38724727838258 | 28.5477876663208 | 29.230409860611 | 30.9620499610901 | 30.7116687297821 | 7.38724727838258 | 178.5477876663208 | 54.230409860611005 | 30.9620499610901 | 105.7116687297821 | |
7.39444732446288 | 28.5582602024079 | 29.2469680309296 | 30.9810996055603 | 30.7359397411347 | 7.39444732446288 | 178.5582602024079 | 54.2469680309296 | 30.9810996055603 | 105.7359397411347 | |
7.40164737054317 | 28.568583726883 | 29.265958070755 | 31.0011863708496 | 30.7617604732514 | 7.40164737054317 | 178.568583726883 | 54.265958070755005 | 31.0011863708496 | 105.7617604732514 | |
7.40884741662347 | 28.5777151584625 | 29.2866408824921 | 31.0236155986786 | 30.7896256446839 | 7.40884741662347 | 178.5777151584625 | 54.2866408824921 | 31.0236155986786 | 105.7896256446839 | |
7.41604746270376 | 28.5849153995514 | 29.3081223964691 | 31.0498476028443 | 30.8198273181915 | 7.41604746270376 | 178.5849153995514 | 54.3081223964691 | 31.0498476028443 | 105.8198273181915 | |
7.42324750878406 | 28.5899341106415 | 29.3296575546265 | 31.081223487854 | 30.8522164821625 | 7.42324750878406 | 178.5899341106415 | 54.3296575546265 | 31.081223487854 | 105.8522164821625 | |
7.43044755486435 | 28.5931706428528 | 29.3509781360626 | 31.1184763908386 | 30.8862864971161 | 7.43044755486435 | 178.5931706428528 | 54.3509781360626 | 31.1184763908386 | 105.8862864971161 | |
7.43764760094465 | 28.5956919193268 | 29.3723106384278 | 31.161493062973 | 30.921345949173 | 7.43764760094465 | 178.5956919193268 | 54.3723106384278 | 31.161493062973 | 105.921345949173 | |
7.44484764702494 | 28.5989582538605 | 29.3939292430878 | 31.2094569206238 | 30.9569001197815 | 7.44484764702494 | 178.5989582538605 | 54.3939292430878 | 31.2094569206238 | 105.9569001197815 | |
7.45204769310524 | 28.6044001579285 | 29.4156193733216 | 31.2611877918244 | 30.992740392685 | 7.45204769310524 | 178.6044001579285 | 54.415619373321604 | 31.2611877918244 | 105.992740392685 | |
7.45924773918553 | 28.6128699779511 | 29.4365465641022 | 31.3153982162476 | 31.0287058353424 | 7.45924773918553 | 178.6128699779511 | 54.4365465641022 | 31.3153982162476 | 106.02870583534241 | |
7.46644778526583 | 28.6244094371796 | 29.4556200504303 | 31.3706457614899 | 31.0646653175354 | 7.46644778526583 | 178.6244094371796 | 54.4556200504303 | 31.3706457614899 | 106.0646653175354 | |
7.47364783134612 | 28.6383807659149 | 29.4720649719238 | 31.4251720905304 | 31.1006247997284 | 7.47364783134612 | 178.6383807659149 | 54.4720649719238 | 31.4251720905304 | 106.1006247997284 | |
7.48084787742642 | 28.6537110805512 | 29.485547542572 | 31.4770221710205 | 31.1368525028229 | 7.48084787742642 | 178.6537110805512 | 54.485547542572 | 31.4770221710205 | 106.1368525028229 | |
7.48804792350671 | 28.6692023277283 | 29.4959664344788 | 31.5245807170868 | 31.1736941337586 | 7.48804792350671 | 178.6692023277283 | 54.4959664344788 | 31.5245807170868 | 106.1736941337586 | |
7.49524796958701 | 28.6836862564087 | 29.503345489502 | 31.5669417381287 | 31.2113344669342 | 7.49524796958701 | 178.6836862564087 | 54.503345489501996 | 31.5669417381287 | 106.2113344669342 | |
7.5024480156673 | 28.6960601806641 | 29.5079350471497 | 31.6040277481079 | 31.2494456768036 | 7.5024480156673 | 178.6960601806641 | 54.5079350471497 | 31.6040277481079 | 106.2494456768036 | |
7.5096480617476 | 28.7054896354676 | 29.5106410980225 | 31.6363215446472 | 31.2872648239136 | 7.5096480617476 | 178.7054896354676 | 54.510641098022504 | 31.6363215446472 | 106.2872648239136 | |
7.51684810782789 | 28.711611032486 | 29.5130670070648 | 31.6646575927735 | 31.3237428665161 | 7.51684810782789 | 178.711611032486 | 54.513067007064805 | 31.6646575927735 | 106.3237428665161 | |
7.52404815390819 | 28.7145972251892 | 29.5169055461884 | 31.6900968551636 | 31.3576757907868 | 7.52404815390819 | 178.7145972251892 | 54.5169055461884 | 31.6900968551636 | 106.3576757907868 | |
7.53124819998848 | 28.7150800228119 | 29.5231759548187 | 31.7139744758606 | 31.3879191875458 | 7.53124819998848 | 178.7150800228119 | 54.5231759548187 | 31.7139744758606 | 106.3879191875458 | |
7.53844824606878 | 28.7139177322388 | 29.5319378376007 | 31.7375659942627 | 31.4135432243347 | 7.53844824606878 | 178.7139177322388 | 54.5319378376007 | 31.7375659942627 | 106.4135432243347 | |
7.54564829214907 | 28.7119448184967 | 29.5424461364746 | 31.7618250846863 | 31.4340829849243 | 7.54564829214907 | 178.7119448184967 | 54.542446136474595 | 31.7618250846863 | 106.4340829849243 | |
7.55284833822937 | 28.7099599838257 | 29.5536398887634 | 31.7870676517487 | 31.4495921134949 | 7.55284833822937 | 178.7099599838257 | 54.5536398887634 | 31.7870676517487 | 106.4495921134949 | |
7.56004838430966 | 28.7086606025696 | 29.5644462108612 | 31.8131268024445 | 31.460702419281 | 7.56004838430966 | 178.7086606025696 | 54.5644462108612 | 31.8131268024445 | 106.460702419281 | |
7.56724843038996 | 28.708416223526 | 29.5737385749817 | 31.8395793437958 | 31.4684331417084 | 7.56724843038996 | 178.708416223526 | 54.573738574981704 | 31.8395793437958 | 106.4684331417084 | |
7.57444847647025 | 28.7090659141541 | 29.5804262161255 | 31.8659842014313 | 31.4740657806397 | 7.57444847647025 | 178.7090659141541 | 54.5804262161255 | 31.8659842014313 | 106.4740657806397 | |
7.58164852255055 | 28.7099123001099 | 29.5838057994843 | 31.8919360637665 | 31.4789235591889 | 7.58164852255055 | 178.7099123001099 | 54.583805799484296 | 31.8919360637665 | 106.4789235591889 | |
7.58884856863084 | 28.7099301815033 | 29.5838534832001 | 31.9172084331513 | 31.4841091632843 | 7.58884856863084 | 178.7099301815033 | 54.5838534832001 | 31.9172084331513 | 106.4841091632843 | |
7.59604861471114 | 28.7081956863404 | 29.5813262462616 | 31.9418072700501 | 31.4903676509857 | 7.59604861471114 | 178.7081956863404 | 54.5813262462616 | 31.9418072700501 | 106.4903676509857 | |
7.60324866079143 | 28.7041604518891 | 29.5775234699249 | 31.9659411907196 | 31.4980804920197 | 7.60324866079143 | 178.7041604518891 | 54.5775234699249 | 31.9659411907196 | 106.4980804920197 | |
7.61044870687173 | 28.6976158618927 | 29.5735836029053 | 31.9897770881653 | 31.5074384212494 | 7.61044870687173 | 178.6976158618927 | 54.5735836029053 | 31.9897770881653 | 106.5074384212494 | |
7.61764875295202 | 28.6886811256409 | 29.5700788497925 | 32.0132374763489 | 31.5185487270355 | 7.61764875295202 | 178.6886811256409 | 54.570078849792495 | 32.0132374763489 | 106.5185487270355 | |
7.62484879903232 | 28.6779165267945 | 29.5669615268707 | 32.0360124111176 | 31.5315425395966 | 7.62484879903232 | 178.6779165267945 | 54.5669615268707 | 32.0360124111176 | 106.5315425395966 | |
7.63204884511261 | 28.6664307117462 | 29.563719034195 | 32.0578813552857 | 31.5465331077576 | 7.63204884511261 | 178.66643071174622 | 54.563719034195 | 32.0578813552857 | 106.5465331077576 | |
7.63924889119291 | 28.6555647850037 | 29.5599222183228 | 32.0788204669953 | 31.5635800361634 | 7.63924889119291 | 178.6555647850037 | 54.5599222183228 | 32.0788204669953 | 106.5635800361634 | |
7.6464489372732 | 28.6464333534241 | 29.5554459095001 | 32.0989072322846 | 31.5826117992401 | 7.6464489372732 | 178.6464333534241 | 54.5554459095001 | 32.0989072322846 | 106.5826117992401 | |
7.6536489833535 | 28.6394417285919 | 29.5505106449127 | 32.1182191371918 | 31.6033065319061 | 7.6536489833535 | 178.6394417285919 | 54.5505106449127 | 32.1182191371918 | 106.6033065319061 | |
7.66084902943379 | 28.6343455314636 | 29.545521736145 | 32.1368277072907 | 31.6250920295716 | 7.66084902943379 | 178.6343455314636 | 54.545521736145005 | 32.1368277072907 | 106.6250920295716 | |
7.66804907551409 | 28.6306381225586 | 29.5409917831421 | 32.1549534797669 | 31.6471695899964 | 7.66804907551409 | 178.6306381225586 | 54.540991783142104 | 32.1549534797669 | 106.6471695899964 | |
7.67524912159438 | 28.6279022693634 | 29.5374870300293 | 32.1729958057404 | 31.6686153411865 | 7.67524912159438 | 178.6279022693634 | 54.5374870300293 | 32.1729958057404 | 106.6686153411865 | |
7.68244916767468 | 28.6258161067963 | 29.5355200767517 | 32.1911811828613 | 31.688517332077 | 7.68244916767468 | 178.6258161067963 | 54.535520076751695 | 32.1911811828613 | 106.688517332077 | |
7.68964921375497 | 28.6241531372071 | 29.535323381424 | 32.2093307971955 | 31.7062258720398 | 7.68964921375497 | 178.6241531372071 | 54.535323381424 | 32.2093307971955 | 106.7062258720398 | |
7.69684925983527 | 28.6229491233826 | 29.5364737510681 | 32.2268426418305 | 31.7215323448181 | 7.69684925983527 | 178.6229491233826 | 54.5364737510681 | 32.2268426418305 | 106.7215323448181 | |
7.70404930591556 | 28.6227703094483 | 29.5379102230072 | 32.242900133133 | 31.7348718643189 | 7.70404930591556 | 178.6227703094483 | 54.5379102230072 | 32.242900133133 | 106.7348718643189 | |
7.71124935199586 | 28.6245882511139 | 29.5383214950562 | 32.2568237781525 | 31.7471444606781 | 7.71124935199586 | 178.6245882511139 | 54.538321495056195 | 32.2568237781525 | 106.7471444606781 | |
7.71844939807615 | 28.6293566226959 | 29.536497592926 | 32.2680532932282 | 31.7595064640045 | 7.71844939807615 | 178.6293566226959 | 54.536497592926 | 32.2680532932282 | 106.7595064640045 | |
7.72564944415645 | 28.6373913288117 | 29.5317649841309 | 32.2761714458466 | 31.7730784416199 | 7.72564944415645 | 178.6373913288117 | 54.5317649841309 | 32.2761714458466 | 106.7730784416199 | |
7.73284949023674 | 28.648191690445 | 29.5240879058838 | 32.2810113430023 | 31.7887723445893 | 7.73284949023674 | 178.648191690445 | 54.5240879058838 | 32.2810113430023 | 106.7887723445893 | |
7.74004953631704 | 28.6607027053833 | 29.5139610767365 | 32.2828829288483 | 31.807154417038 | 7.74004953631704 | 178.6607027053833 | 54.5139610767365 | 32.2828829288483 | 106.80715441703799 | |
7.74724958239733 | 28.6734819412232 | 29.5023381710053 | 32.2827458381653 | 31.8284511566162 | 7.74724958239733 | 178.6734819412232 | 54.5023381710053 | 32.2827458381653 | 106.8284511566162 | |
7.75444962847763 | 28.6848723888397 | 29.4904410839081 | 32.282042503357 | 31.8525016307831 | 7.75444962847763 | 178.6848723888397 | 54.490441083908095 | 32.282042503357 | 106.8525016307831 | |
7.76164967455792 | 28.6930918693543 | 29.4794142246247 | 32.2823882102967 | 31.8790316581726 | 7.76164967455792 | 178.6930918693543 | 54.479414224624705 | 32.2823882102967 | 106.87903165817261 | |
7.76884972063821 | 28.6966562271118 | 29.4700562953949 | 32.2853684425354 | 31.9078862667084 | 7.76884972063821 | 178.6966562271118 | 54.4700562953949 | 32.2853684425354 | 106.9078862667084 | |
7.77604976671851 | 28.6948144435883 | 29.4627606868744 | 32.2923362255097 | 31.9391071796417 | 7.77604976671851 | 178.6948144435883 | 54.462760686874404 | 32.2923362255097 | 106.9391071796417 | |
7.78324981279881 | 28.6878108978272 | 29.4574558734894 | 32.304334640503 | 31.9728255271912 | 7.78324981279881 | 178.6878108978272 | 54.4574558734894 | 32.304334640503 | 106.9728255271912 | |
7.7904498588791 | 28.6766946315766 | 29.4538259506226 | 32.3219358921051 | 32.0091187953949 | 7.7904498588791 | 178.6766946315766 | 54.4538259506226 | 32.3219358921051 | 107.0091187953949 | |
7.79764990495939 | 28.6628127098084 | 29.4513463973999 | 32.3451697826386 | 32.0479452610016 | 7.79764990495939 | 178.6628127098084 | 54.4513463973999 | 32.3451697826386 | 107.0479452610016 | |
7.80484995103969 | 28.6474406719208 | 29.4493436813355 | 32.3736190795899 | 32.0891201496124 | 7.80484995103969 | 178.6474406719208 | 54.4493436813355 | 32.3736190795899 | 107.0891201496124 | |
7.81204999711998 | 28.631728887558 | 29.4471561908722 | 32.4066638946533 | 32.1322023868561 | 7.81204999711998 | 178.631728887558 | 54.4471561908722 | 32.4066638946533 | 107.13220238685611 | |
7.81925004320028 | 28.6168336868286 | 29.4443190097809 | 32.4436843395233 | 32.1763634681702 | 7.81925004320028 | 178.6168336868286 | 54.4443190097809 | 32.4436843395233 | 107.1763634681702 | |
7.82645008928057 | 28.6039352416992 | 29.4405400753021 | 32.4840903282166 | 32.2205126285553 | 7.82645008928057 | 178.6039352416992 | 54.440540075302096 | 32.4840903282166 | 107.2205126285553 | |
7.83365013536087 | 28.5940647125244 | 29.4357657432556 | 32.5275301933289 | 32.2636365890503 | 7.83365013536087 | 178.5940647125244 | 54.4357657432556 | 32.5275301933289 | 107.2636365890503 | |
7.84085018144116 | 28.5878300666809 | 29.4301092624665 | 32.5739681720734 | 32.3050498962403 | 7.84085018144116 | 178.5878300666809 | 54.4301092624665 | 32.5739681720734 | 107.30504989624029 | |
7.84805022752146 | 28.5853028297425 | 29.4237136840821 | 32.623702287674 | 32.3444306850434 | 7.84805022752146 | 178.5853028297425 | 54.4237136840821 | 32.623702287674 | 107.34443068504339 | |
7.85525027360175 | 28.5863220691681 | 29.4168770313263 | 32.6771676540375 | 32.3814928531647 | 7.85525027360175 | 178.5863220691681 | 54.4168770313263 | 32.6771676540375 | 107.3814928531647 | |
7.86245031968205 | 28.5907089710236 | 29.4101059436798 | 32.7344238758087 | 32.4157953262329 | 7.86245031968205 | 178.5907089710236 | 54.410105943679795 | 32.7344238758087 | 107.41579532623291 | |
7.86965036576234 | 28.5983085632324 | 29.4040501117707 | 32.7948689460755 | 32.4467778205872 | 7.86965036576234 | 178.5983085632324 | 54.4040501117707 | 32.7948689460755 | 107.4467778205872 | |
7.87685041184264 | 28.6087453365326 | 29.3995022773743 | 32.8572034835816 | 32.4739396572113 | 7.87685041184264 | 178.6087453365326 | 54.399502277374296 | 32.8572034835816 | 107.4739396572113 | |
7.88405045792293 | 28.6211848258972 | 29.3971121311188 | 32.9196035861969 | 32.4967086315155 | 7.88405045792293 | 178.6211848258972 | 54.3971121311188 | 32.9196035861969 | 107.4967086315155 | |
7.89125050400323 | 28.6344826221466 | 29.3972849845886 | 32.9798758029938 | 32.5141906738281 | 7.89125050400323 | 178.6344826221466 | 54.3972849845886 | 32.9798758029938 | 107.5141906738281 | |
7.89845055008352 | 28.6475419998169 | 29.4002413749695 | 33.0353379249573 | 32.5250446796417 | 7.89845055008352 | 178.6475419998169 | 54.4002413749695 | 33.0353379249573 | 107.5250446796417 |