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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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6.46564138010484 | 28.9102733135224 | 29.7573149204254 | 34.6968650817871 | 32.076644897461 | 6.46564138010484 | 178.9102733135224 | 54.7573149204254 | 34.6968650817871 | 107.076644897461 | |
6.47284142618513 | 28.9201140403748 | 29.7496676445008 | 34.6626996994019 | 32.0618510246277 | 6.47284142618513 | 178.9201140403748 | 54.7496676445008 | 34.6626996994019 | 107.0618510246277 | |
6.48004147226543 | 28.9305090904236 | 29.7435700893402 | 34.6242547035217 | 32.0469856262207 | 6.48004147226543 | 178.9305090904236 | 54.743570089340196 | 34.6242547035217 | 107.0469856262207 | |
6.48724151834572 | 28.9399683475495 | 29.7397613525391 | 34.5810413360596 | 32.0325016975403 | 6.48724151834572 | 178.9399683475495 | 54.739761352539105 | 34.5810413360596 | 107.0325016975403 | |
6.49444156442601 | 28.9470672607422 | 29.7386646270752 | 34.5324814319611 | 32.0190787315369 | 6.49444156442601 | 178.9470672607422 | 54.738664627075195 | 34.5324814319611 | 107.0190787315369 | |
6.50164161050631 | 28.9509534835816 | 29.7401607036591 | 34.4784259796143 | 32.0073187351227 | 6.50164161050631 | 178.9509534835816 | 54.7401607036591 | 34.4784259796143 | 107.00731873512271 | |
6.50884165658661 | 28.9515674114228 | 29.7437369823456 | 34.419322013855 | 31.9971978664398 | 6.50884165658661 | 178.9515674114228 | 54.743736982345595 | 34.419322013855 | 106.9971978664398 | |
6.5160417026669 | 28.9495885372162 | 29.7487914562226 | 34.3559324741364 | 31.9879293441773 | 6.5160417026669 | 178.9495885372162 | 54.748791456222605 | 34.3559324741364 | 106.9879293441773 | |
6.52324174874719 | 28.9460420608521 | 29.7547578811646 | 34.2889726161957 | 31.978315114975 | 6.52324174874719 | 178.9460420608521 | 54.7547578811646 | 34.2889726161957 | 106.978315114975 | |
6.53044179482749 | 28.9419293403626 | 29.7610938549042 | 34.2190980911255 | 31.967306137085 | 6.53044179482749 | 178.9419293403626 | 54.7610938549042 | 34.2190980911255 | 106.967306137085 | |
6.53764184090778 | 28.9379954338074 | 29.7672092914582 | 34.1468811035156 | 31.9540560245514 | 6.53764184090778 | 178.9379954338074 | 54.7672092914582 | 34.1468811035156 | 106.95405602455139 | |
6.54484188698808 | 28.9349853992462 | 29.7726094722748 | 34.0725839138031 | 31.9377660751343 | 6.54484188698808 | 178.93498539924622 | 54.7726094722748 | 34.0725839138031 | 106.9377660751343 | |
6.55204193306837 | 28.9336383342743 | 29.7771751880646 | 33.9958310127259 | 31.9175481796265 | 6.55204193306837 | 178.9336383342743 | 54.777175188064604 | 33.9958310127259 | 106.9175481796265 | |
6.55924197914867 | 28.9345979690552 | 29.7813415527344 | 33.9152812957764 | 31.8927824497223 | 6.55924197914867 | 178.9345979690552 | 54.7813415527344 | 33.9152812957764 | 106.8927824497223 | |
6.56644202522896 | 28.9381802082062 | 29.7858774662018 | 33.8290333747864 | 31.8635165691376 | 6.56644202522896 | 178.9381802082062 | 54.785877466201796 | 33.8290333747864 | 106.8635165691376 | |
6.57364207130926 | 28.9442598819733 | 29.7913789749146 | 33.7352573871613 | 31.8305313587189 | 6.57364207130926 | 178.9442598819733 | 54.7913789749146 | 33.7352573871613 | 106.8305313587189 | |
6.58084211738955 | 28.9525747299195 | 29.7978818416596 | 33.6326718330384 | 31.7952036857605 | 6.58084211738955 | 178.9525747299195 | 54.7978818416596 | 33.6326718330384 | 106.7952036857605 | |
6.58804216346985 | 28.9634346961975 | 29.8049449920655 | 33.5207939147949 | 31.7593276500702 | 6.58804216346985 | 178.9634346961975 | 54.8049449920655 | 33.5207939147949 | 106.7593276500702 | |
6.59524220955014 | 28.9780855178833 | 29.8118889331818 | 33.400160074234 | 31.7249178886414 | 6.59524220955014 | 178.9780855178833 | 54.811888933181805 | 33.400160074234 | 106.7249178886414 | |
6.60244225563044 | 28.9981424808502 | 29.8180282115936 | 33.2725405693054 | 31.6940724849701 | 6.60244225563044 | 178.9981424808502 | 54.8180282115936 | 33.2725405693054 | 106.69407248497009 | |
6.60964230171073 | 29.0246367454529 | 29.822701215744 | 33.1410527229309 | 31.6686153411865 | 6.60964230171073 | 179.0246367454529 | 54.822701215744004 | 33.1410527229309 | 106.6686153411865 | |
6.61684234779103 | 29.0570139884949 | 29.8252701759339 | 33.0099046230316 | 31.6497147083283 | 6.61684234779103 | 179.0570139884949 | 54.825270175933895 | 33.0099046230316 | 106.6497147083283 | |
6.62404239387132 | 29.0932059288025 | 29.8253774642945 | 32.8836441040039 | 31.6378116607666 | 6.62404239387132 | 179.0932059288025 | 54.825377464294505 | 32.8836441040039 | 106.6378116607666 | |
6.63124243995162 | 29.1307151317597 | 29.8233807086945 | 32.7664971351624 | 31.6327631473541 | 6.63124243995162 | 179.1307151317597 | 54.8233807086945 | 32.7664971351624 | 106.6327631473541 | |
6.63844248603191 | 29.1670203208924 | 29.8203945159912 | 32.6620638370514 | 31.6339790821076 | 6.63844248603191 | 179.1670203208924 | 54.8203945159912 | 32.6620638370514 | 106.6339790821076 | |
6.64564253211221 | 29.1990101337433 | 29.8178613185883 | 32.5729846954346 | 31.6406190395355 | 6.64564253211221 | 179.1990101337433 | 54.8178613185883 | 32.5729846954346 | 106.6406190395355 | |
6.6528425781925 | 29.2226374149323 | 29.8169374465943 | 32.5008034706116 | 31.6517531871796 | 6.6528425781925 | 179.2226374149323 | 54.816937446594295 | 32.5008034706116 | 106.6517531871796 | |
6.6600426242728 | 29.2336404323578 | 29.8180341720581 | 32.4458003044129 | 31.6666185855866 | 6.6600426242728 | 179.2336404323578 | 54.8180341720581 | 32.4458003044129 | 106.6666185855866 | |
6.66724267035309 | 29.2295753955841 | 29.8207998275757 | 32.406896352768 | 31.6848158836365 | 6.66724267035309 | 179.2295753955841 | 54.8207998275757 | 32.406896352768 | 106.6848158836365 | |
6.67444271643339 | 29.2113602161408 | 29.8243403434754 | 32.3818802833557 | 31.7062437534332 | 6.67444271643339 | 179.2113602161408 | 54.8243403434754 | 32.3818802833557 | 106.7062437534332 | |
6.68164276251368 | 29.1825771331787 | 29.8275470733643 | 32.3678612709046 | 31.7309200763703 | 6.68164276251368 | 179.1825771331787 | 54.8275470733643 | 32.3678612709046 | 106.7309200763703 | |
6.68884280859398 | 29.1473090648651 | 29.8292279243469 | 32.3616266250611 | 31.7587494850159 | 6.68884280859398 | 179.1473090648651 | 54.829227924346895 | 32.3616266250611 | 106.7587494850159 | |
6.69604285467427 | 29.1089713573456 | 29.8284888267517 | 32.3602080345154 | 31.7893922328949 | 6.69604285467427 | 179.1089713573456 | 54.828488826751695 | 32.3602080345154 | 106.7893922328949 | |
6.70324290075457 | 29.0705740451813 | 29.8249244689942 | 32.3612749576569 | 31.8223297595978 | 6.70324290075457 | 179.0705740451813 | 54.8249244689942 | 32.3612749576569 | 106.8223297595978 | |
6.71044294683486 | 29.0353655815125 | 29.8187255859375 | 32.363361120224 | 31.8569779396057 | 6.71044294683486 | 179.0353655815125 | 54.8187255859375 | 32.363361120224 | 106.8569779396057 | |
6.71764299291516 | 29.0066778659821 | 29.8106908798218 | 32.3658227920532 | 31.892877817154 | 6.71764299291516 | 179.0066778659821 | 54.8106908798218 | 32.3658227920532 | 106.892877817154 | |
6.72484303899545 | 28.9863646030426 | 29.8019945621491 | 32.3689103126526 | 31.9300830364228 | 6.72484303899545 | 178.9863646030426 | 54.801994562149105 | 32.3689103126526 | 106.9300830364228 | |
6.73204308507575 | 28.973937034607 | 29.7937989234925 | 32.373857498169 | 31.9694519042969 | 6.73204308507575 | 178.973937034607 | 54.7937989234925 | 32.373857498169 | 106.9694519042969 | |
6.73924313115604 | 28.967410326004 | 29.7869563102722 | 32.3828995227814 | 32.0127010345459 | 6.73924313115604 | 178.967410326004 | 54.7869563102722 | 32.3828995227814 | 107.0127010345459 | |
6.74644317723634 | 28.9647817611695 | 29.7818899154663 | 32.3990762233734 | 32.0623636245728 | 6.74644317723634 | 178.9647817611695 | 54.7818899154663 | 32.3990762233734 | 107.0623636245728 | |
6.75364322331663 | 28.9648830890656 | 29.7785639762879 | 32.4255526065827 | 32.1213126182556 | 6.75364322331663 | 178.9648830890656 | 54.7785639762879 | 32.4255526065827 | 107.1213126182556 | |
6.76084326939693 | 28.9670050144196 | 29.7766566276551 | 32.4650049209595 | 32.1921229362488 | 6.76084326939693 | 178.9670050144196 | 54.7766566276551 | 32.4650049209595 | 107.19212293624881 | |
6.76804331547722 | 28.9700388908386 | 29.7758340835571 | 32.5193464756012 | 32.2764575481415 | 6.76804331547722 | 178.9700388908386 | 54.7758340835571 | 32.5193464756012 | 107.27645754814151 | |
6.77524336155752 | 28.9723873138428 | 29.7758221626282 | 32.5897037982941 | 32.3743939399719 | 6.77524336155752 | 178.9723873138428 | 54.7758221626282 | 32.5897037982941 | 107.3743939399719 | |
6.78244340763781 | 28.9726436138153 | 29.7765254974365 | 32.6763987541199 | 32.4842214584351 | 6.78244340763781 | 178.9726436138153 | 54.776525497436495 | 32.6763987541199 | 107.4842214584351 | |
6.78964345371811 | 28.970205783844 | 29.7780632972717 | 32.7785789966583 | 32.6023995876312 | 6.78964345371811 | 178.970205783844 | 54.7780632972717 | 32.7785789966583 | 107.6023995876312 | |
6.7968434997984 | 28.9652526378632 | 29.7805428504944 | 32.8936457633972 | 32.7237069606781 | 6.7968434997984 | 178.9652526378632 | 54.7805428504944 | 32.8936457633972 | 107.7237069606781 | |
6.8040435458787 | 28.9585053920746 | 29.7839760780335 | 33.0169498920441 | 32.8415870666504 | 6.8040435458787 | 178.95850539207458 | 54.783976078033504 | 33.0169498920441 | 107.84158706665039 | |
6.81124359195899 | 28.9508283138275 | 29.7883212566376 | 33.1420958042145 | 32.9487502574921 | 6.81124359195899 | 178.95082831382751 | 54.7883212566376 | 33.1420958042145 | 107.9487502574921 | |
6.81844363803929 | 28.9432048797608 | 29.793518781662 | 33.2615733146668 | 33.037942647934 | 6.81844363803929 | 178.9432048797608 | 54.793518781662 | 33.2615733146668 | 108.037942647934 | |
6.82564368411958 | 28.9368152618408 | 29.7994673252106 | 33.3674550056458 | 33.1028044223786 | 6.82564368411958 | 178.9368152618408 | 54.7994673252106 | 33.3674550056458 | 108.1028044223786 | |
6.83284373019988 | 28.9328038692475 | 29.8059225082398 | 33.4518969058991 | 33.1384062767029 | 6.83284373019988 | 178.9328038692475 | 54.8059225082398 | 33.4518969058991 | 108.13840627670291 | |
6.84004377628017 | 28.932136297226 | 29.8123300075531 | 33.5076272487641 | 33.1416308879853 | 6.84004377628017 | 178.932136297226 | 54.8123300075531 | 33.5076272487641 | 108.1416308879853 | |
6.84724382236047 | 28.9354085922241 | 29.817944765091 | 33.5287272930145 | 33.1112861633301 | 6.84724382236047 | 178.9354085922241 | 54.817944765091 | 33.5287272930145 | 108.1112861633301 | |
6.85444386844076 | 28.9427101612091 | 29.8221647739411 | 33.511620759964 | 33.0480039119721 | 6.85444386844076 | 178.9427101612091 | 54.8221647739411 | 33.511620759964 | 108.0480039119721 | |
6.86164391452106 | 28.9536833763123 | 29.8247814178467 | 33.4556996822357 | 32.9542398452759 | 6.86164391452106 | 178.9536833763123 | 54.8247814178467 | 33.4556996822357 | 107.95423984527591 | |
6.86884396060135 | 28.9676010608673 | 29.8258125782013 | 33.3633661270142 | 32.8340172767639 | 6.86884396060135 | 178.9676010608673 | 54.8258125782013 | 33.3633661270142 | 107.8340172767639 | |
6.87604400668165 | 28.9836168289185 | 29.825222492218 | 33.2394778728485 | 32.6925277709961 | 6.87604400668165 | 178.9836168289185 | 54.825222492218 | 33.2394778728485 | 107.6925277709961 | |
6.88324405276194 | 29.0010154247284 | 29.8228025436402 | 33.0905556678772 | 32.5356781482697 | 6.88324405276194 | 179.0010154247284 | 54.8228025436402 | 33.0905556678772 | 107.53567814826971 | |
6.89044409884223 | 29.019159078598 | 29.8184394836426 | 32.9240500926972 | 32.3695480823517 | 6.89044409884223 | 179.019159078598 | 54.8184394836426 | 32.9240500926972 | 107.3695480823517 | |
6.89764414492253 | 29.0372371673584 | 29.8124670982361 | 32.7476561069489 | 32.1999311447144 | 6.89764414492253 | 179.0372371673584 | 54.8124670982361 | 32.7476561069489 | 107.1999311447144 | |
6.90484419100283 | 29.0542721748352 | 29.8056602478028 | 32.5687110424042 | 32.0321679115296 | 6.90484419100283 | 179.0542721748352 | 54.8056602478028 | 32.5687110424042 | 107.0321679115296 | |
6.91204423708312 | 29.0692448616028 | 29.7988474369049 | 32.3935270309448 | 31.8709433078766 | 6.91204423708312 | 179.0692448616028 | 54.7988474369049 | 32.3935270309448 | 106.8709433078766 | |
6.91924428316341 | 29.0816307067871 | 29.7926306724549 | 32.2269260883332 | 31.7200541496277 | 6.91924428316341 | 179.0816307067871 | 54.792630672454905 | 32.2269260883332 | 106.7200541496277 | |
6.92644432924371 | 29.0915727615357 | 29.7874450683594 | 32.0720911026001 | 31.5822303295136 | 6.92644432924371 | 179.0915727615357 | 54.7874450683594 | 32.0720911026001 | 106.5822303295136 | |
6.933644375324 | 29.0997207164765 | 29.7837913036347 | 31.9306313991547 | 31.4590334892273 | 6.933644375324 | 179.0997207164765 | 54.7837913036347 | 31.9306313991547 | 106.4590334892273 | |
6.9408444214043 | 29.1069090366364 | 29.782372713089 | 31.80291056633 | 31.3510119915009 | 6.9408444214043 | 179.1069090366364 | 54.782372713089 | 31.80291056633 | 106.3510119915009 | |
6.94804446748459 | 29.1140258312226 | 29.7838091850281 | 31.6884696483612 | 31.2579452991486 | 6.94804446748459 | 179.1140258312226 | 54.783809185028105 | 31.6884696483612 | 106.2579452991486 | |
6.95524451356489 | 29.1220903396607 | 29.7882318496704 | 31.5863490104676 | 31.179016828537 | 6.95524451356489 | 179.1220903396607 | 54.788231849670396 | 31.5863490104676 | 106.179016828537 | |
6.96244455964518 | 29.1323363780976 | 29.7952055931092 | 31.4952373504639 | 31.1128556728363 | 6.96244455964518 | 179.1323363780976 | 54.7952055931092 | 31.4952373504639 | 106.1128556728363 | |
6.96964460572548 | 29.1458606719971 | 29.8040330410004 | 31.4136743545532 | 31.0577750205994 | 6.96964460572548 | 179.1458606719971 | 54.8040330410004 | 31.4136743545532 | 106.0577750205994 | |
6.97684465180577 | 29.1631937026978 | 29.8141360282898 | 31.3402175903321 | 31.0119032859802 | 6.97684465180577 | 179.1631937026978 | 54.814136028289795 | 31.3402175903321 | 106.0119032859802 | |
6.98404469788607 | 29.1839361190796 | 29.825222492218 | 31.2736868858338 | 30.973368883133 | 6.98404469788607 | 179.1839361190796 | 54.825222492218 | 31.2736868858338 | 105.97336888313299 | |
6.99124474396636 | 29.2068779468537 | 29.8372328281403 | 31.2134921550751 | 30.9403479099274 | 6.99124474396636 | 179.2068779468537 | 54.8372328281403 | 31.2134921550751 | 105.9403479099274 | |
6.99844479004666 | 29.230397939682 | 29.8501908779144 | 31.1595261096955 | 30.9110760688782 | 6.99844479004666 | 179.230397939682 | 54.8501908779144 | 31.1595261096955 | 105.9110760688782 | |
7.00564483612695 | 29.2526602745056 | 29.8641383647919 | 31.1119019985199 | 30.884051322937 | 7.00564483612695 | 179.25266027450562 | 54.8641383647919 | 31.1119019985199 | 105.884051322937 | |
7.01284488220725 | 29.2716503143311 | 29.8793911933899 | 31.0706377029419 | 30.8582186698914 | 7.01284488220725 | 179.2716503143311 | 54.8793911933899 | 31.0706377029419 | 105.8582186698914 | |
7.02004492828754 | 29.2852282524109 | 29.8965871334076 | 31.0355305671692 | 30.8331251144409 | 7.02004492828754 | 179.2852282524109 | 54.8965871334076 | 31.0355305671692 | 105.8331251144409 | |
7.02724497436784 | 29.2913019657135 | 29.9165368080139 | 31.0061812400818 | 30.8087766170502 | 7.02724497436784 | 179.2913019657135 | 54.9165368080139 | 31.0061812400818 | 105.8087766170502 | |
7.03444502044813 | 29.2882561683655 | 29.939877986908 | 30.9820175170899 | 30.7854235172272 | 7.03444502044813 | 179.2882561683655 | 54.939877986908 | 30.9820175170899 | 105.7854235172272 | |
7.04164506652843 | 29.27525639534 | 29.9668252468109 | 30.9622883796692 | 30.7634830474854 | 7.04164506652843 | 179.27525639534 | 54.9668252468109 | 30.9622883796692 | 105.76348304748541 | |
7.04884511260872 | 29.2521893978119 | 29.9970209598541 | 30.9459328651428 | 30.7435810565949 | 7.04884511260872 | 179.2521893978119 | 54.9970209598541 | 30.9459328651428 | 105.7435810565949 | |
7.05604515868902 | 29.2195081710816 | 30.0297975540161 | 30.9317708015442 | 30.7264268398285 | 7.05604515868902 | 179.2195081710816 | 55.0297975540161 | 30.9317708015442 | 105.72642683982849 | |
7.06324520476931 | 29.1782319545746 | 30.0644218921662 | 30.9186816215515 | 30.7125329971314 | 7.06324520476931 | 179.17823195457458 | 55.0644218921662 | 30.9186816215515 | 105.7125329971314 | |
7.07044525084961 | 29.1298985481262 | 30.1001429557801 | 30.9058070182801 | 30.7018756866455 | 7.07044525084961 | 179.1298985481262 | 55.1001429557801 | 30.9058070182801 | 105.70187568664551 | |
7.0776452969299 | 29.0765225887299 | 30.1359593868256 | 30.8928310871124 | 30.6938707828522 | 7.0776452969299 | 179.07652258872992 | 55.135959386825604 | 30.8928310871124 | 105.6938707828522 | |
7.0848453430102 | 29.0202975273133 | 30.1703035831452 | 30.8799505233765 | 30.6876420974732 | 7.0848453430102 | 179.0202975273133 | 55.1703035831452 | 30.8799505233765 | 105.6876420974732 | |
7.09204538909049 | 28.9632737636566 | 30.2009403705597 | 30.8678328990936 | 30.682373046875 | 7.09204538909049 | 178.9632737636566 | 55.2009403705597 | 30.8678328990936 | 105.682373046875 | |
7.09924543517079 | 28.907322883606 | 30.2251398563385 | 30.8574259281159 | 30.6775391101837 | 7.09924543517079 | 178.907322883606 | 55.2251398563385 | 30.8574259281159 | 105.6775391101837 | |
7.10644548125108 | 28.8541674613953 | 30.2401602268219 | 30.8496177196503 | 30.6727945804596 | 7.10644548125108 | 178.8541674613953 | 55.2401602268219 | 30.8496177196503 | 105.6727945804596 | |
7.11364552733138 | 28.8053333759308 | 30.2434086799622 | 30.8449327945709 | 30.6679129600525 | 7.11364552733138 | 178.8053333759308 | 55.2434086799622 | 30.8449327945709 | 105.6679129600525 | |
7.12084557341167 | 28.7619352340698 | 30.2325248718262 | 30.8434247970581 | 30.6626856327057 | 7.12084557341167 | 178.7619352340698 | 55.2325248718262 | 30.8434247970581 | 105.6626856327057 | |
7.12804561949197 | 28.7245571613312 | 30.2056550979614 | 30.844646692276 | 30.6570529937744 | 7.12804561949197 | 178.7245571613312 | 55.2056550979614 | 30.844646692276 | 105.6570529937744 | |
7.13524566557226 | 28.6933481693268 | 30.1619231700897 | 30.8475494384766 | 30.6511521339417 | 7.13524566557226 | 178.6933481693268 | 55.1619231700897 | 30.8475494384766 | 105.65115213394171 | |
7.14244571165256 | 28.6681413650513 | 30.1021754741669 | 30.8506548404694 | 30.6451261043549 | 7.14244571165256 | 178.6681413650513 | 55.1021754741669 | 30.8506548404694 | 105.6451261043549 | |
7.14964575773285 | 28.648453950882 | 30.0290822982788 | 30.8522164821625 | 30.6388676166535 | 7.14964575773285 | 178.64845395088201 | 55.0290822982788 | 30.8522164821625 | 105.6388676166535 | |
7.15684580381315 | 28.6333084106446 | 29.9465775489807 | 30.8506011962891 | 30.6319892406464 | 7.15684580381315 | 178.6333084106446 | 54.9465775489807 | 30.8506011962891 | 105.6319892406464 | |
7.16404584989344 | 28.6212861537934 | 29.8590481281281 | 30.8448314666748 | 30.6240439414978 | 7.16404584989344 | 178.6212861537934 | 54.8590481281281 | 30.8448314666748 | 105.6240439414978 | |
7.17124589597374 | 28.6108016967774 | 29.7709703445435 | 30.834835767746 | 30.6149005889893 | 7.17124589597374 | 178.6108016967774 | 54.7709703445435 | 30.834835767746 | 105.6149005889893 | |
7.17844594205403 | 28.6005020141602 | 29.6866655349732 | 30.8214247226715 | 30.6048512458801 | 7.17844594205403 | 178.6005020141602 | 54.6866655349732 | 30.8214247226715 | 105.6048512458801 |