Laurel and Hardy 2 mean data and simulations

Selected Cell

Cell:

Value:

Metadata Biomass_not_used Starch_not_used M+F_not_used Biomass_without clock Starch_without clock M+F _without clock plot_LH2_biomass_and_carbon_poo

Data	Laurel and Hardy 2 (Nov 2014)
Temperature	18.5	Note lower temperature in this experiment
Light	145
Photoperiod	12
CO2 level	42
Photosynthetic 'efficiency'	0.79
Water content	0.9	WT
	0.8925	lsf1
	0.8804	prr7/prr9
Starch synthesis	0.55 of TiMet AGPase activity data

Model	FM 1.1.1.1 (FM v1 + toy starch + fixed starch synthesis + malate/fumarate), now "FMv2" from DDS sims in last sheet
	cvRMSEs all from YHC sims, not used


With the clock	NOT USED
			micromol CO2/g FW/h	micromol CO2/g FW/h	micromol CO2/cm2/h	micromol CO2/cm2/h	lsf-1 (A)
	cvRMSE	nRMSE	AperFW	RperFW	Aperarea	Rperarea	P.ksX = 10*P.ksX;
Col	0.1282	0.0537	57.4716	-10.2513	1.1876	-0.2102	P.kdS = 0.045*P.kdS;
lsf1 (A)	0.2058	0.0857	61.6882	-12.5139	1.188	-0.2388
lsf1 (B)	0.1188	0.0495	61.6767	-12.4874	1.1879	-0.2383	lsf-1 (B)
prr7prr9 (starch only)	0.1895	0.0791	68.6643	-12.4131	1.1877	-0.2129	P.ksX = 10*P.ksX;
prr7prr9 (starch +m+f)	0.0691	0.0288	68.7539	-12.2638	1.1879	-0.2101	P.kdS = 0.05*P.kdS;


Model	FM 1.2.1 (FM v1 + fixed starch synthesis + malate/fumarate)
	This is the version used, but with DDS updates
Without the clock
	incorrect data, old sims		micromol CO2/g FW/h	micromol CO2/g FW/h	micromol CO2/cm2/h	micromol CO2/cm2/h
	cvRMSE	nRMSE	AperFW	RperFW	Aperarea	Rperarea
Col	0.1583	0.0663	57.4541	-10.7902	1.1877	-0.2213
lsf1	0.1256	0.0523	61.7416	-11.399	1.1879	-0.2174
prr7prr9 (starch only)	0.454	0.1894	68.6455	-12.8806	1.1877	-0.2211
prr7prr9 (starch +m+f)	0.2142	0.0894	68.6816	-12.729	1.1878	-0.2182

Sheets in this worksheet	Contents
[various]_not_used	Earlier simulation versions, discounted when the effect of low growth temperature on prr7prr9 became clear, so the clock model for 21C could not be applied to this experiment
Biomass_without clock [model]	Fresh (not dry) rosette leaf biomass, measured in samples of 5 plants each on multiple days, as mean and SD; simulation outputs from FMv2 for Col Wild Type plants, lsf1, and two simulations for prr7prr9, where the mutation affects only starch degradation, or both starch degradation and malate/fumarate store mobilisation - all with calibrated not default starch degradation
Starch_without clock	Starch levels in carbon units (not C6) measured on 27-28 days, mean and SD, simulations as above
M + F_without clock	Malate and fumarate levels in carbon units (not C4) measured on 27-28 days, mean and SD, simulations as above
plot_LH2_biomass_and_carbon_poo[ls]	Simulation data from FMv2 runs in the conditions above, from the Matlab output file

Credits
Experimental setup, physiological data, sampling and model construction	Yin Hoon Chew, Millar group University of Edinburgh
Model refinement and final simulations	Daniel Seaton, Millar group University of Edinburgh
Starch, M+F and other metabolite analysis	Virginie Mengin et al, Stitt group, MPIMP

100

	Biomass (Data)			Biomass (simulation)
Day	21	30	39	Day	Col	lsf-1 (A)	lsf-1 (B)	prr7/prr9 (starch defect)	prr7/prr9 (starch+store)
Col	0.02037	0.11558	0.65736	0.041666666666666664	0	0	0	0	0
SD	0.005347	0.012648	0.126642	0.08333333333333333	0	0	0	0	0
lsf-1	0.01514	0.10316	0.550836	0.125	0	0	0	0	0
SD	0.003585	0.012487	0.164038	0.16666666666666666	0	0	0	0	0
prr7/prr9	0.01486	0.0809	0.454443	0.20833333333333331	0	0	0	0	0
SD	0.001797	0.013998	0.055393	0.24999999999999997	0	0	0	0	0
				0.29166666666666663	0	0	0	0	0
				0.3333333333333333	0	0	0	0	0
				0.375	0	0	0	0	0
				0.4166666666666667	0	0	0	0	0
				0.45833333333333337	0	0	0	0	0
				0.5	0	0	0	0	0
				0.5416666666666666	0	0	0	0	0
				0.5833333333333333	0	0	0	0	0
				0.6249999999999999	0	0	0	0	0
				0.6666666666666665	0	0	0	0	0
				0.7083333333333331	0	0	0	0	0
				0.7499999999999998	0	0	0	0	0
				0.7916666666666664	0	0	0	0	0
				0.833333333333333	0	0	0	0	0
				0.8749999999999997	0	0	0	0	0
				0.9166666666666663	0	0	0	0	0
				0.9583333333333329	0	0	0	0	0
				0.9999999999999996	0	0	0	0	0
				1.0416666666666663	0	0	0	0	0
				1.083333333333333	0	0	0	0	0
				1.1249999999999998	0	0	0	0	0
				1.1666666666666665	0	0	0	0	0
				1.2083333333333333	0	0	0	0	0
				1.25	0	0	0	0	0
				1.2916666666666667	0	0	0	0	0
				1.3333333333333335	0	0	0	0	0
				1.3750000000000002	0	0	0	0	0
				1.416666666666667	0	0	0	0	0
				1.4583333333333337	0	0	0	0	0
				1.5000000000000004	0	0	0	0	0
				1.5416666666666672	0	0	0	0	0
				1.583333333333334	0	0	0	0	0
				1.6250000000000007	0	0	0	0	0
				1.6666666666666674	0	0	0	0	0
				1.7083333333333341	0	0	0	0	0
				1.7500000000000009	0	0	0	0	0
	NOTE - MF sim removed to test PPT link…			1.7916666666666676	0	0	0	0	0
				1.8333333333333344	0	0	0	0	0
				1.875000000000001	0	0	0	0	0
				1.9166666666666679	0	0	0	0	0
				1.9583333333333346	0	0	0	0	0
				2.0000000000000013	0	0	0	0	0
				2.041666666666668	0	0	0	0	0
				2.0833333333333344	0	0	0	0	0
				2.125000000000001	0	0	0	0	0
				2.1666666666666674	0	0	0	0	0
				2.208333333333334	0	0	0	0	0
				2.2500000000000004	0	0	0	0	0
				2.291666666666667	0	0	0	0	0
				2.3333333333333335	0	0	0	0	0
				2.375	0	0	0	0	0
				2.4166666666666665	0	0	0	0	0
				2.458333333333333	0	0	0	0	0
				2.4999999999999996	0	0	0	0	0
				2.541666666666666	0	0	0	0	0
				2.5833333333333326	0	0	0	0	0
				2.624999999999999	0	0	0	0	0
				2.6666666666666656	0	0	0	0	0
				2.708333333333332	0	0	0	0	0
				2.7499999999999987	0	0	0	0	0
				2.791666666666665	0	0	0	0	0
				2.8333333333333317	0	0	0	0	0
				2.8749999999999982	0	0	0	0	0
				2.9166666666666647	0	0	0	0	0
				2.9583333333333313	0	0	0	0	0
				2.999999999999998	0	0	0	0	0
				3.0416666666666643	0	0	0	0	0
				3.083333333333331	0	0	0	0	0
				3.1249999999999973	0	0	0	0	0
				3.166666666666664	0	0	0	0	0
				3.2083333333333304	0	0	0	0	0
				3.249999999999997	0	0	0	0	0
				3.2916666666666634	0	0	0	0	0
				3.33333333333333	0	0	0	0	0
				3.3749999999999964	0	0	0	0	0
				3.416666666666663	0	0	0	0	0
				3.4583333333333295	0	0	0	0	0
				3.499999999999996	0	0	0	0	0
				3.5416666666666625	0	0	0	0	0
				3.583333333333329	0	0	0	0	0
				3.6249999999999956	0	0	0	0	0
				3.666666666666662	0	0	0	0	0
				3.7083333333333286	0	0	0	0	0
				3.749999999999995	0	0	0	0	0
				3.7916666666666616	0	0	0	0	0
				3.833333333333328	0	0	0	0	0
				3.8749999999999947	0	0	0	0	0
				3.916666666666661	0	0	0	0	0
				3.9583333333333277	0	0	0	0	0
				3.9999999999999942	0	0	0	0	0
				4.041666666666661	0	0	0	0	0
				4.083333333333328	0	0	0	0	0

In C6					in C6						In C				in C
	Starch (data)				Starch (simulation)							Starch (data)			Starch (simulation)
Hour	0	12	24		Hour	Col	lsf-1 (A)	lsf-1 (B)	prr7/prr9 (starch defect)	prr7/prr9 (starch+store)	Hour	0	12	24	Hour	Col	lsf-1 (A)	lsf-1 (B)	prr7/prr9 (starch defect)	prr7/prr9 (starch+store)
Col	2.16	40.97	3.24		0	2.9308	32.2891	26.2307	19.3871	19.457	Col	12.96	245.82	19.44	0	17.5848	193.7346	157.3842	116.3226	116.742
SD	0.41	2.66	0.42		1	6.5204	35.6654	29.7057	23.4158	23.4911	SD	2.46	15.96	2.52	1	39.1224	213.99239999999998	178.2342	140.4948	140.9466
lsf-1	29.7	58.44	36.09		2	10.0364	38.9708	33.1086	27.3611	27.4406	lsf-1	178.2	350.64	216.54000000000002	2	60.2184	233.82479999999998	198.65160000000003	164.16660000000002	164.6436
SD	1.19	1.28	4.18		3	13.4827	42.2083	36.4422	31.2268	31.3099	SD	7.14	7.68	25.08	3	80.8962	253.2498	218.6532	187.3608	187.8594
prr7/prr9	3.6	47.64	7.15		4	16.8605	45.3799	39.7083	35.0148	35.1013	prr7/prr9	21.6	285.84000000000003	42.900000000000006	4	101.16299999999998	272.2794	238.2498	210.0888	210.6078
SD	0.42	5.38	0.71		5	20.1709	48.4867	42.9081	38.7265	38.8163	SD	2.52	32.28	4.26	5	121.02539999999999	290.9202	257.4486	232.359	232.8978
					6	23.4152	51.5298	46.0426	42.363	42.456					6	140.4912	309.1788	276.2556	254.178	254.73600000000002
				lsf-1 (A)	7	26.5946	54.5104	49.1131	45.9258	46.0219					7	159.5676	327.06239999999997	294.6786	275.5548	276.1314
				P.ksX = 10*P.ksX;	8	29.7105	57.4296	52.1209	49.4165	49.5152					8	178.263	344.5776	312.7254	296.499	297.0912
				P.kdS = 0.045*P.kdS;	9	32.7681	60.2887	55.0671	52.8363	52.9377					9	196.60859999999997	361.7322	330.4026	317.0178	317.6262
					10	35.7726	63.104	57.9703	56.2053	56.3014					10	214.63559999999998	378.624	347.8218	337.2318	337.8084
				lsf-1 (B)	11	38.7205	65.8719	60.8208	59.5111	59.6086					11	232.323	395.2314	364.9248	357.0666	357.65160000000003
				P.ksX = 10*P.ksX;	12	41.6132	68.5885	63.6185	62.7553	62.854					12	249.67919999999998	411.53099999999995	381.711	376.5318	377.124
				P.kdS = 0.05*P.kdS;	13	37.5832	66.3255	61.2478	58.5713	58.6792					13	225.49919999999997	397.95300000000003	367.4868	351.4278	352.0752
					14	33.676	63.9773	58.7966	54.4754	54.5902					14	202.056	383.86379999999997	352.77959999999996	326.8524	327.5412
					15	29.9022	61.5404	56.2615	50.4759	50.5953					15	179.41320000000002	369.2424	337.56899999999996	302.85540000000003	303.5718
					16	26.2649	59.0032	53.6314	46.5735	46.6954					16	157.5894	354.0192	321.7884	279.44100000000003	280.1724
					17	22.7737	56.3592	50.8997	42.7762	42.8987					17	136.6422	338.15520000000004	305.39820000000003	256.6572	257.3922
					18	19.4266	53.5742	48.0339	39.0739	39.1951					18	116.5596	321.4452	288.2034	234.4434	235.17059999999998
					19	16.2347	50.6291	45.0151	35.4738	35.5921					19	97.4082	303.7746	270.0906	212.84279999999998	213.5526
					20	13.2071	47.4887	41.8085	31.9787	32.0924					20	79.24260000000001	284.9322	250.851	191.8722	192.5544
					21	10.354	44.1027	38.364	28.5917	28.6993					21	62.123999999999995	264.6162	230.18399999999997	171.5502	172.19580000000002
					22	7.6856	40.3947	34.6036	25.3165	25.4166					22	46.1136	242.3682	207.6216	151.899	152.4996
					23	5.2065	36.2444	30.4003	22.1572	22.2487					23	31.239	217.4664	182.4018	132.9432	133.4922
					24	2.9026	31.4371	25.5157	19.1191	19.2008					24	17.4156	188.6226	153.0942	114.71459999999999	115.2048

In C4				in C4						In C					in C
	M+F (data)			M+F (simulation)							M+F (data)				M+F (simulation)
Hour	0	12	24	Hour	Col	lsf-1 (A)	lsf-1 (B)	prr7/prr9 (starch defect)	prr7/prr9 (starch+store)	Hour	0	12	24		Hour	Col	lsf-1 (A)	lsf-1 (B)	prr7/prr9 (starch defect)	prr7/prr9 (starch+store)
Col	6.37	16.89	6.5	0	6.8444	7.4286	7.4097	8.2191	29.8967	Col	25.48	67.56	26		0	27.3776	29.7144	29.6388	32.8764	119.5868
SD	0.97	0.95	0.67	1	7.8216	8.4792	8.4599	9.3876	30.7048	SD	3.88	3.8	2.68		1	31.2864	33.9168	33.8396	37.5504	122.8192
lsf-1	6.81	18.8	7.54	2	8.7783	9.5079	9.4883	10.5317	31.4944	lsf-1	27.24	75.2	30.16		2	35.1132	38.0316	37.9532	42.1268	125.9776
SD	1.44	0.44	0.35	3	9.7158	10.5157	10.4958	11.6526	32.2664	SD	5.76	1.76	1.4		3	38.8632	42.0628	41.9832	46.6104	129.0656
prr7/prr9	14.64	34.49	16.11	4	10.6344	11.503	11.4829	12.7508	33.0216	prr7/prr9	58.56	137.96	64.44		4	42.5376	46.012	45.9316	51.0032	132.0864
SD	1.15	4.59	2.24	5	11.5343	12.4704	12.45	13.8267	33.7602	SD	4.6	18.36	8.96		5	46.1372	49.8816	49.8	55.3068	135.0408
				6	12.416	13.418	13.3975	14.8808	34.4825						6	49.664	53.672	53.59	59.5232	137.93
				7	13.2797	14.3462	14.3255	15.9133	35.1889						7	53.1188	57.3848	57.302	63.6532	140.7556
				8	14.1259	15.2555	15.2347	16.9248	35.8796						8	56.5036	61.022	60.9388	67.6992	143.5184
				9	14.9567	16.1463	16.1253	17.9157	36.5549						9	59.8268	64.5852	64.5012	71.6628	146.2196
				10	15.7741	17.0228	17.0027	18.8926	37.2225						10	63.0964	68.0912	68.0108	75.5704	148.89
				11	16.5764	17.8843	17.8642	19.8512	37.8827						11	66.3056	71.5372	71.4568	79.4048	151.5308
				12	17.3637	18.7298	18.7097	20.792	38.5312						12	69.4548	74.9192	74.8388	83.168	154.1248
				13	16.4374	17.7763	17.7548	19.6973	37.7619						13	65.7496	71.1052	71.0192	78.7892	151.0476
				14	15.5187	16.8215	16.799	18.609	36.9967						14	62.0748	67.286	67.196	74.436	147.9868
				15	14.6085	15.8672	15.844	17.5282	36.2384						15	58.434	63.4688	63.376	70.1128	144.9536
				16	13.7064	14.9134	14.8897	16.4546	35.487						16	54.8256	59.6536	59.5588	65.8184	141.948
				17	12.8141	13.9623	13.9386	15.3904	34.7476						17	51.2564	55.8492	55.7544	61.5616	138.9904
				18	11.9276	13.0102	12.9867	14.3309	34.0108						18	47.7104	52.0408	51.9468	57.3236	136.0432
				19	11.048	12.0588	12.0358	13.2777	33.2804						19	44.192	48.2352	48.1432	53.1108	133.1216
				20	10.1748	11.1081	11.0859	12.2304	32.5562						20	40.6992	44.4324	44.3436	48.9216	130.2248
				21	9.3077	10.1583	10.1371	11.1888	31.8382						21	37.2308	40.6332	40.5484	44.7552	127.3528
				22	8.4462	9.2092	9.1893	10.1525	31.1262						22	33.7848	36.8368	36.7572	40.61	124.5048
				23	7.5898	8.2608	8.2422	9.1212	30.4202						23	30.3592	33.0432	32.9688	36.4848	121.6808
				24	6.7375	7.3126	7.2952	8.0946	29.7201						24	26.95	29.2504	29.1808	32.3784	118.8804

											M+F + starch (data)
										Hour	0	12	24	Turnover
										Col	38.44	313.38	45.44	0.8550003191014104

										lsf-1	205.44	425.84	246.70000000000002	0.42067443171144087

										prr7/prr9	80.16	423.80000000000007	107.34	0.7467201510146295

100

	Biomass (Data)					Biomass (simulation) - from plot_LH2 sheet, FW columns
Day	21	30	39			Day	Col	lsf-1	prr7/prr9 (starch defect)	prr7/prr9 (starch+store)
Col	0.02037	0.11558	0.65736			0.041666666666666664	0	0	0	0
SD	0.005347	0.012648	0.126642			0.08333333333333333	0	0	0	0
lsf-1	0.01514	0.10316	0.550836			0.125	0	0	0	0
SD	0.003585	0.012487	0.164038			0.16666666666666666	0	0	0	0
prr7/prr9	0.01486	0.0809	0.454443	0.6913152610441766	of WT mass	0.20833333333333331	0	0	0	0
SD	0.001797	0.013998	0.055393			0.24999999999999997	0	0	0	0
				n=5 each		0.29166666666666663	0	0	0	0
				two-tailed p = 0.0111		0.3333333333333333	0	0	0	0
						0.375	0	0	0	0
						0.4166666666666667	0	0	0	0
						0.45833333333333337	0	0	0	0
						0.5	0	0	0	0
						0.5416666666666666	0	0	0	0
						0.5833333333333333	0	0	0	0
						0.6249999999999999	0	0	0	0
						0.6666666666666665	0	0	0	0
						0.7083333333333331	0	0	0	0
						0.7499999999999998	0	0	0	0
						0.7916666666666664	0	0	0	0
						0.833333333333333	0	0	0	0
						0.8749999999999997	0	0	0	0
						0.9166666666666663	0	0	0	0
						0.9583333333333329	0	0	0	0
						0.9999999999999996	0	0	0	0
						1.0416666666666663	0	0	0	0
						1.083333333333333	0	0	0	0
						1.1249999999999998	0	0	0	0
						1.1666666666666665	0	0	0	0
						1.2083333333333333	0	0	0	0
						1.25	0	0	0	0
						1.2916666666666667	0	0	0	0
						1.3333333333333335	0	0	0	0
						1.3750000000000002	0	0	0	0
						1.416666666666667	0	0	0	0
						1.4583333333333337	0	0	0	0
						1.5000000000000004	0	0	0	0
						1.5416666666666672	0	0	0	0
						1.583333333333334	0	0	0	0
						1.6250000000000007	0	0	0	0
						1.6666666666666674	0	0	0	0
						1.7083333333333341	0	0	0	0
						1.7500000000000009	0	0	0	0
						1.7916666666666676	0	0	0	0
						1.8333333333333344	0	0	0	0
						1.875000000000001	0	0	0	0
						1.9166666666666679	0	0	0	0
						1.9583333333333346	0	0	0	0
						2.0000000000000013	0	0	0	0
						2.041666666666668	0	0	0	0
						2.0833333333333344	0	0	0	0
						2.125000000000001	0	0	0	0
						2.1666666666666674	0	0	0	0
						2.208333333333334	0	0	0	0
						2.2500000000000004	0	0	0	0
						2.291666666666667	0	0	0	0
						2.3333333333333335	0	0	0	0
						2.375	0	0	0	0
						2.4166666666666665	0	0	0	0
						2.458333333333333	0	0	0	0
						2.4999999999999996	0	0	0	0
						2.541666666666666	0	0	0	0
						2.5833333333333326	0	0	0	0
						2.624999999999999	0	0	0	0
						2.6666666666666656	0	0	0	0
						2.708333333333332	0	0	0	0
						2.7499999999999987	0	0	0	0
						2.791666666666665	0	0	0	0
						2.8333333333333317	0	0	0	0
						2.8749999999999982	0	0	0	0
						2.9166666666666647	0	0	0	0
						2.9583333333333313	0	0	0	0
						2.999999999999998	0	0	0	0
						3.0416666666666643	0	0	0	0
						3.083333333333331	0	0	0	0
						3.1249999999999973	0	0	0	0
						3.166666666666664	0	0	0	0
						3.2083333333333304	0	0	0	0
						3.249999999999997	0	0	0	0
						3.2916666666666634	0	0	0	0
						3.33333333333333	0	0	0	0
						3.3749999999999964	0	0	0	0
						3.416666666666663	0	0	0	0
						3.4583333333333295	0	0	0	0
						3.499999999999996	0	0	0	0
						3.5416666666666625	0	0	0	0
						3.583333333333329	0	0	0	0
						3.6249999999999956	0	0	0	0
						3.666666666666662	0	0	0	0
						3.7083333333333286	0	0	0	0
						3.749999999999995	0	0	0	0
						3.7916666666666616	0	0	0	0
						3.833333333333328	0	0	0	0
						3.8749999999999947	0	0	0	0
						3.916666666666661	0	0	0	0
						3.9583333333333277	0	0	0	0
						3.9999999999999942	0	0	0	0
						4.041666666666661	0	0	0	0
						4.083333333333328	0	0	0	0

In C6				in C6	plot_LH2 sheet "starchC6"	Rows 674-698			In C					in C
	Starch (data)			Starch (simulation)						Starch (data)				Starch (simulation)
Hour	0	12	24	Hour	Col	lsf-1	prr7/prr9 (starch defect)	prr7/prr9 (starch+store)	Hour	0	12	24	ave 0,24	Hour	Col	lsf-1	prr7/prr9 (starch defect)	prr7/prr9 (starch+store)
Col	2.16	40.97	3.24	0	3.31519511745447	29.2257449279761	5.61693722223772	5.63365322642316	Col	12.96	245.82	19.44	16.200000000000003	0	19.891170704726818	175.3544695678566	33.70162333342632	33.80191935853896
SD	0.41	2.66	0.42	1	6.89306587769821	32.7444244219323	9.88358587500085	9.90599776751708	SD	2.46	15.96	2.52		1	41.35839526618926	196.4665465315938	59.3015152500051	59.435986605102485
lsf-1	29.7	58.44	36.09	2	10.3986161101028	36.189336351648	14.0638272154432	14.0913637673246	lsf-1	178.2	350.64	216.54000000000002	197.37	2	62.3916966606168	217.13601810988803	84.3829632926592	84.5481826039476
SD	1.19	1.28	4.18	3	13.834675611444	39.5636900331128	18.1611141009643	18.1930853059582	SD	7.14	7.68	25.08		3	83.008053668664	237.38214019867678	108.96668460578582	109.1585118357492
prr7/prr9	3.6	47.64	7.15	4	17.2024298567157	42.8695675674034	22.1768619287783	22.2131267318548	prr7/prr9	21.6	285.84000000000003	42.900000000000006	32.25	4	103.2145791402942	257.2174054044204	133.06117157266982	133.2787603911288
SD	0.42	5.38	0.71	5	20.5030438676149	46.1081405938744	26.1124616712077	26.152882194281	SD	2.52	32.28	4.26		5	123.0182632056894	276.64884356324643	156.6747700272462	156.91729316568598
				6	23.7376625963352	49.2805604984478	29.9692803342451	30.0137218158339						6	142.4259755780112	295.68336299068676	179.8156820054706	180.0823308950034
				7	26.9076620976702	52.3879587965205	33.7489312482259	33.7969921424227						7	161.4459725860212	314.327752779123	202.4935874893554	202.7819528545362
				8	30.014280711792	55.431657653684	37.4529253196544	37.5044563634941						8	180.085684270752	332.589945922104	224.7175519179264	225.0267381809646
				9	33.0635205025613	58.4129397848349	41.0881181197959	41.1376690826814						9	198.38112301536782	350.4776387090094	246.52870871877542	246.8260144960884
				10	36.059335021208	61.3474999248483	44.6603407233507	44.7123999247866						10	216.356010127248	368.0849995490898	267.96204434010417	268.2743995487196
				11	38.9986840516074	64.2323466250662	48.1652616110627	48.2196516690804						11	233.9921043096444	385.39407975039717	288.99156966637617	289.3179100144824
				12	41.8829562216658	67.0636265961162	51.6045403799933	51.6611184852489						12	251.2977373299948	402.38175957669716	309.62724227995983	309.9667109114934
				13	38.5722653268839	63.801586529328	47.6554982641411	47.7211517621636						13	231.43359196130342	382.809519175968	285.9329895848466	286.3269105729816
				14	35.2762619480777	60.5487521556468	43.7236208956719	43.7959918032185						14	211.65757168846622	363.2925129338808	262.3417253740314	262.775950819311
				15	31.997843159932	57.3101378032396	39.8124992364067	39.8893544333585						15	191.987058959592	343.8608268194376	238.87499541844022	239.33612660015098
				16	28.7367832512898	54.0855733993597	35.9218681561121	36.0010185130064						16	172.4206995077388	324.5134403961582	215.53120893667258	216.0061110780384
				17	25.4968175418249	50.8826627628455	32.056437642588	32.1357070322189						17	152.9809052509494	305.295976577073	192.338625855528	192.81424219331342
				18	22.270369965744	47.6874174778641	28.2067496748345	28.2840607340606						18	133.622219794464	286.1245048671846	169.24049804900702	169.7043644043636
				19	19.0603814837543	44.5055251908415	24.3764933159407	24.4497871287713						19	114.36228890252579	267.03315114504903	146.25895989564418	146.69872277262778
				20	15.8666432054632	41.336824875577	20.5654227776845	20.6326809309391						20	95.1998592327792	248.020949253462	123.39253666610699	123.79608558563459
				21	12.6889634631446	38.1811826655304	16.7733136115375	16.832557232714						21	76.13378077886759	229.0870959931824	100.63988166922499	100.995343396284
				22	9.52715364297956	35.0384676851584	12.9999449122324	13.0492338207293						22	57.16292185787736	210.23080611095043	77.99966947339439	78.29540292437581
				23	6.38102818705189	31.9085503422613	9.24509932715182	9.28253120563874						23	38.28616912231134	191.45130205356782	55.47059596291092	55.69518723383244
				24	3.25040453369068	28.7913026777138	5.50856298756935	5.53227257343402						24	19.50242720214408	172.7478160662828	33.051377925416105	33.19363544060412

In C4				in C4 - from plot_LH2 sheet, 'malatefumarateC4' columns, rows from 674 = hour from 671					In C					in C
	M+F (data)			M+F (simulation)						M+F (data)				M+F (simulation)
Hour	0	12	24	Hour	Col	lsf-1	prr7/prr9 (starch defect)	prr7/prr9 (starch+store)	Hour	0	12	24	ave 0,24	Hour	Col	lsf-1	prr7/prr9 (starch defect)	prr7/prr9 (starch+store)
Col	6.37	16.89	6.5	0	6.83685278818308	7.595236183861	8.20962693446628	29.7140905332828	Col	25.48	67.56	26	25.740000000000002	0	27.34741115273232	30.380944735444	32.83850773786512	118.8563621331312
SD	0.97	0.95	0.67	1	7.81257574649926	8.67043385984802	9.38058160651899	30.5274616250254	SD	3.88	3.8	2.68		1	31.25030298599704	34.68173543939208	37.52232642607596	122.1098465001016
lsf-1	6.81	18.8	7.54	2	8.76823397069703	9.72317391416758	10.5274360061838	31.3225640024816	lsf-1	27.24	75.2	30.16	28.7	2	35.07293588278812	38.89269565667032	42.1097440247352	125.2902560099264
SD	1.44	0.44	0.35	3	9.70464915175301	10.7544246077939	11.651195561325	32.1002672139625	SD	5.76	1.76	1.4		3	38.81859660701204	43.0176984311756	46.6047822453	128.40106885585
prr7/prr9	14.64	34.49	16.11	4	10.6221501388358	11.7648159460475	12.7522549695558	32.861032068551	prr7/prr9	58.56	137.96	64.44	61.5	4	42.4886005553432	47.05926378419	51.0090198782232	131.444128274204
SD	1.15	4.59	2.24	5	11.521060092275	12.7547047060702	13.831002100366	33.6051504595424	SD	4.6	18.36	8.96		5	46.0842403691	51.0188188242808	55.324008401464	134.4206018381696
				6	12.4016965912488	13.7244415008043	14.887818124371	34.3329091804877						6	49.6067863649952	54.8977660032172	59.551272497484	137.3316367219508
				7	13.2644469780881	14.6743708953336	15.923158592332	35.044590025073						7	53.0577879123524	58.6974835813344	63.692634369328	140.178360100292
				8	14.1096577059052	15.6048945645418	16.9374488026577	35.7406018186551						8	56.4386308236208	62.4195782581672	67.7497952106308	142.9624072746204
				9	14.9398551762625	16.5164022642658	17.9334807171272	36.4214453808234						9	59.75942070505	66.0656090570632	71.7339228685088	145.6857815232936
				10	15.7564441748338	17.4133706700182	18.9133895978918	37.0993974612015						10	63.0257766993352	69.6534826800728	75.6535583915672	148.397589844806
				11	16.5577901897037	18.2949984436245	19.8750029004366	37.7652504901323						11	66.2311607588148	73.179993774498	79.5000116017464	151.0610019605292
				12	17.3442729480428	19.1602220501997	20.8187776662694	38.419350979595						12	69.3770917921712	76.6408882007988	83.2751106650776	153.67740391838
				13	16.4345950689927	18.1645150615739	19.7274576592491	37.6614366260625						13	65.7383802759708	72.6580602462956	78.9098306369964	150.64574650425
				14	15.528566919242	17.1717105841808	18.6404457155032	36.9044084948931						14	62.114267676968	68.6868423367232	74.5617828620128	147.6176339795724
				15	14.6274181778376	16.1832383299995	17.5592225090537	36.151144256276						15	58.5096727113504	64.732953319998	70.2368900362148	144.604577025104
				16	13.7310842107751	15.1990469492853	16.4837118401882	35.4015889428475						16	54.9243368431004	60.7961877971412	65.9348473607528	141.60635577139
				17	12.8414935327975	14.2212579999593	15.4162281590483	34.6610126179886						17	51.36597413119	56.8850319998372	61.6649126361932	138.6440504719544
				18	11.9550248601797	13.2459420333061	14.3524292295252	33.9201709820853						18	47.8200994407188	52.9837681332244	57.4097169181008	135.6806839283412
				19	11.0731242027797	12.2746940227622	13.294050759255	33.1828634398035						19	44.2924968111188	49.0987760910488	53.17620303702	132.731453759214
				20	10.195730403188	11.3074655590359	12.2410206144171	32.4490328356508						20	40.782921612752	45.2298622361436	48.9640824576684	129.7961313426032
				21	9.32279026713574	10.3442158750252	11.1932761857291	31.7186415573292						21	37.29116106854296	41.3768635001008	44.7731047429164	126.8745662293168
				22	8.45425143108857	9.38490507850324	10.1507558334216	30.9916524598038						22	33.81700572435428	37.53962031401296	40.6030233336864	123.9666098392152
				23	7.59006237919115	8.42949368148228	9.1133989082454	30.2680289504342						23	30.3602495167646	33.71797472592912	36.4535956329816	121.0721158017368
				24	6.73017242688376	7.47794269709312	8.08114573248314	29.547734979776						24	26.92068970753504	29.91177078837248	32.32458292993256	118.190939919104

100

simulation	WT	WT	WT	WT	WT	WT	WT	WT	WT	WT	WT	WT	WT	lsf1	lsf1	lsf1	lsf1	lsf1	lsf1	lsf1	lsf1	lsf1	lsf1	lsf1	lsf1	lsf1	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9_noMF	prr7prr9	prr7prr9	prr7prr9	prr7prr9	prr7prr9	prr7prr9	prr7prr9	prr7prr9	prr7prr9	prr7prr9	prr7prr9	prr7prr9	prr7prr9
variable	Total_shoot	Starch_carbon	MF_carbon	starch	sucrose	NPP	Gas_exchange_perFW	starch_perFW	sucrose_perFW	FW	starchC6	malatefumarateC4	sugar	Total_shoot	Starch_carbon	MF_carbon	starch	sucrose	NPP	Gas_exchange_perFW	starch_perFW	sucrose_perFW	FW	starchC6	malatefumarateC4	sugar	Total_shoot	Starch_carbon	MF_carbon	starch	sucrose	NPP	Gas_exchange_perFW	starch_perFW	sucrose_perFW	FW	starchC6	malatefumarateC4	sugar	Total_shoot	Starch_carbon	MF_carbon	starch	sucrose	NPP	Gas_exchange_perFW	starch_perFW	sucrose_perFW	FW	starchC6	malatefumarateC4	sugar
0	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
1	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
2	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
3	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
4	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
5	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
6	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
7	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
8	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
9	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
10	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
11	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
12	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
13	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
14	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
15	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
16	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
17	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
18	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
19	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
20	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
21	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
22	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
23	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
24	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
25	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
26	0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0				0	0	0	0	0	0				0
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