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| Metadata | Values (examples) | ||||||||
| Asset Title | Kinetics PGM1 | ||||||||
| Uploader | Sofia Doello | ||||||||
| Uploader SEEK ID | |||||||||
| Project | SCyCode | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 1466 | ||||||||
| Assay Title | Kinetics of PGM1 variants | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | enzymatic activity measurements | ||||||||
| Description | Kinetic characterisation of PGM1 variants. Expermental data for enzyme reaction rates with increasing concentrations of glucose-1-phosphate | ||||||||
| Experimentalist | Sofia Doello | ||||||||
| Date | |||||||||
| SOP | https://fairdomhub.org/sops/490 | ||||||||
| Publication (optional) | doi: 10.1101/2021.04.15.439997 | ||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | °C | ||||||||
| Start_value (optional) | 30 | ||||||||
| End_value (optional) | 30 | ||||||||
| Comments | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | ||||||||
| Compound (if concentration) | Glucose-1-phosphate | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 3 | ||||||||
| End_value (optional) | 0.0625 | ||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglucomutase 1 | ||||||||
| EC Number | 5.4.2.2 | ||||||||
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| Concentration | |||||||||
| Glucose-1-phosphate (mM) | Activity (U/mg) | ||||||||
| PGM1 | PGM1 S47D | PGM1 S47A | |||||||
| 3 | 183.3685 | 180.9532 | 182.571 | 1.609851 | 1.559874 | 1.623671 | 94.62782 | 93.56427 | 95.0087 |
| 2 | 181.1129 | 180.6774 | 178.6935 | 1.548387 | 1.258065 | 1.693548 | 97.06452 | 87.67742 | 96 |
| 1 | 146.9516 | 140.7581 | 143.5645 | 1.258065 | 1.016129 | 1.016129 | 75.3871 | 65.08065 | 74.51613 |
| 0.5 | 105.3387 | 101.8065 | 102.7742 | 0.967742 | 0.774194 | 0.725806 | 52.59677 | 47.66129 | 53.70968 |
| 0.25 | 59.95161 | 59.66129 | 57.87097 | 0.580645 | 0.483871 | 0.483871 | 29.32258 | 29.95161 | 31.1129 |
| 0.125 | 33.14516 | 33.48387 | 31.93548 | 0.290323 | 0.193548 | 0.241935 | 17.22581 | 18.33871 | 19.69355 |
| 0.0625 | 17.03226 | 17.03226 | 16.59677 | 9.580645 | 9.483871 | 9.629032 | |||
