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| MRA Titer | CMR Titer | Mutant Selection on 6-TG Plates | ||||||||||||||||||||||
| Replicates | Average | Titer | Replicates | Average | Titer | Plate | Replicates | Total | Growth/No Growth | Total | Mutation Frequency | |||||||||||||
| Accesion Number | MRA Titer Replicate 1 | MRA Titer Replicate 2 | MRA Titer Replicate 3 | MRA Titer Replicate 4 | MRA Titer Replicate Avg. | MRA Titer Titer (pfu) | CMR Colonies Replicate 1 | CMR Colonies Replicate 2 | CMR Colonies Replicate 3 | CMR Colonies Replicate 4 | CMR Colonies Replicate Average | CMR Colonies Titer (cfu) | Titer Efficiency | 6-TG Replicate Plate | 6-TG Replicate 1 | 6-TG Replicate 2 | 6-TG Replicate 3 | 6-TG Replicate 4 | 6-TG Replicate Total | 6-TG Replicate No growth | 6-TG Replicate Confirmed | Mutant Frequency Total | Mutation Frequency | Mutant Frequency per 10^6 |
| 19-599 | 397 | 375 | 387 | 363 | 380.5 | 2283000 | 292 | 334 | 220 | 298 | 286 | 1716000 | 75.16425755584757 | K | 0 | 1 | 1 | 1 | 3 | 1 | 2 | 2 | 1.1655011655011655E-6 | 1.1655011655011656 |
| 19-556 | 548 | 596 | 520 | 604 | 567 | 3402000 | 519 | 462 | 433 | 425 | 459.75 | 2758500 | 81.08465608465607 | CDE | 62 | 72 | 87 | 63 | 284 | 5 | 279 | 279 | 1.0114192495921697E-4 | 101.14192495921696 |
| 19-600 | 368 | 284 | 296 | 360 | 327 | 1962000 | 272 | 312 | 238 | 237 | 264.75 | 1588500 | 80.96330275229357 | K | 2 | 3 | 0 | 1 | 6 | 6 | 6 | 3.777148253068933E-6 | 3.777148253068933 | |
| 19-605 | 516 | 452 | 356 | 472 | 449 | 2694000 | 393 | 332 | 345 | 381 | 362.75 | 2176500 | 80.79064587973274 | FG | 60 | 48 | 38 | 53 | 199 | 8 | 191 | 191 | 8.775557087066391E-5 | 87.7555708706639 |
| 19-1236 | 586 | 562 | 548 | 664 | 590 | 3540000 | 499 | 436 | 416 | 488 | 459.75 | 2758500 | 77.92372881355932 | K | 1 | 2 | 6 | 3 | 12 | 1 | 11 | 11 | 3.987674460757658E-6 | 3.9876744607576584 |
| 19-1163 | 448 | 356 | 452 | 468 | 431 | 2586000 | 304 | 307 | 359 | 363 | 333.25 | 1999500 | 77.3201856148492 | K | 0 | 3 | 4 | 3 | 10 | 2 | 8 | 8 | 4.0010002500625154E-6 | 4.001000250062515 |
| 19-604 | 718 | 626 | 654 | 684 | 670.5 | 4023000 | 382 | 454 | 458 | 424 | 429.5 | 2577000 | 64.05667412378821 | K | 4 | 3 | 3 | 2 | 12 | 2 | 10 | 10 | 3.880481179666279E-6 | 3.880481179666279 |
| 19-733 | 512 | 372 | 332 | 432 | 412 | 2472000 | 240 | 200 | 307 | 224 | 242.75 | 1456500 | 58.91990291262136 | A | 25 | 30 | 15 | 22 | 92 | 7 | 85 | 85 | 5.835907998626845E-5 | 58.35907998626845 |
| 19-774 | 248 | 337 | 325 | 309 | 304.75 | 1828500 | 240 | 190 | 155 | 238 | 205.75 | 1234500 | 67.51435602953241 | K | 0 | 1 | 1 | 1 | 3 | 1 | 2 | 2 | 1.6200891049007696E-6 | 1.6200891049007695 |
| 19-738 | 512 | 456 | 544 | 468 | 495 | 2970000 | 281 | 326 | 298 | 263 | 292 | 1752000 | 58.98989898989899 | HIJ | 55 | 58 | 96 | 88 | 297 | 6 | 291 | 291 | 1.6609589041095891E-4 | 166.0958904109589 |
| 19-777 | 546 | 512 | 496 | 616 | 542.5 | 3255000 | 290 | 304 | 337 | 301 | 308 | 1848000 | 56.774193548387096 | K | 2 | 0 | 0 | 1 | 3 | 0 | 3 | 3 | 1.6233766233766235E-6 | 1.6233766233766234 |
| 19-611 | 684 | 628 | 632 | 548 | 623 | 3738000 | 449 | 435 | 359 | 355 | 399.5 | 2397000 | 64.12520064205457 | QRST | 100 | 98 | 86 | 82 | 366 | 3 | 363 | 363 | 1.5143929912390488E-4 | 151.43929912390487 |
| 19-773 | 580 | 444 | 412 | 504 | 485 | 2910000 | 298 | 351 | 364 | 348 | 340.25 | 2041500 | 70.15463917525773 | K | 2 | 1 | 0 | 6 | 9 | 9 | 9 | 4.40852314474651E-6 | 4.40852314474651 | |
| 19-584 | 229 | 204 | 225 | 221 | 219.75 | 1318500 | 149 | 176 | 162 | 161 | 162 | 972000 | 73.72013651877133 | B | 34 | 19 | 23 | 23 | 99 | 3 | 96 | 96 | 9.876543209876543E-5 | 98.76543209876543 |
| 19-1060 | 484 | 520 | 572 | 564 | 535 | 3210000 | 278 | 344 | 335 | 320 | 319.25 | 1915500 | 59.67289719626169 | K | 2 | 3 | 0 | 2 | 7 | 7 | 7 | 3.6543983294179064E-6 | 3.6543983294179063 | |
| 19-598 | 620 | 660 | 636 | 492 | 602 | 3612000 | 296 | 23 | 213 | 340 | 218 | 1308000 | 36.21262458471761 | H | 3 | 1 | 0 | 0 | 4 | 3 | 1 | 1 | 7.645259938837921E-7 | 0.764525993883792 |
| 19-778 | 584 | 587 | 619 | 634 | 606 | 3636000 | 272 | 312 | 347 | 257 | 297 | 1782000 | 49.00990099009901 | F | 3 | 2 | 1 | 6 | 12 | 1 | 11 | 11 | 6.172839506172839E-6 | 6.172839506172839 |
| 19-194 | 444 | 528 | 486 | 2916000 | 581 | 613 | 524 | 401 | 529.75 | 3178500 | 109.00205761316872 | LMNOP | 104 | 128 | 100 | 93 | 425 | 3 | 422 | 422 | 1.3276702847254994E-4 | 132.76702847254995 | ||
| 19-612 | 276 | 224 | 316 | 340 | 289 | 1734000 | 174 | 167 | 179 | 142 | 165.5 | 993000 | 57.266435986159166 | TUV | 66 | 69 | 68 | 43 | 246 | 5 | 241 | 241 | 2.4269889224572003E-4 | 242.69889224572003 |
| 19-608 | 480 | 480 | 428 | 668 | 514 | 3084000 | 285 | 328 | 279 | 297 | 297.25 | 1783500 | 57.8307392996109 | FG | 55 | 40 | 48 | 45 | 188 | 4 | 184 | 184 | 1.0316792823100645E-4 | 103.16792823100644 |
| 19-554 | 184 | 140 | 186 | 173 | 170.75 | 1024500 | 87 | 82 | 89 | 90 | 87 | 522000 | 50.95168374816984 | A | 33 | 18 | 28 | 18 | 97 | 3 | 94 | 94 | 1.8007662835249042E-4 | 180.07662835249042 |
| 19-1365 | 804 | 956 | 744 | 824 | 832 | 4992000 | 471 | 427 | 372 | 401 | 417.75 | 2506500 | 50.21033653846154 | P | 22 | 19 | 15 | 12 | 68 | 0 | 68 | 68 | 2.7129463395172553E-5 | 27.129463395172554 |
| 19-739 | 420 | 540 | 424 | 404 | 447 | 2682000 | 242 | 289 | 257 | 214 | 250.5 | 1503000 | 56.04026845637584 | HIJK | 81 | 72 | 88 | 87 | 328 | 14 | 314 | 314 | 2.0891550232867597E-4 | 208.91550232867598 |
| 19-602 | 592 | 576 | 423 | 379 | 492.5 | 2955000 | 306 | 292 | 247 | 260 | 276.25 | 1657500 | 56.09137055837563 | K | 0 | 1 | 1 | 3 | 5 | 1 | 4 | 4 | 2.4132730015082956E-6 | 2.4132730015082955 |
| 19-1255 | 532 | 556 | 592 | 520 | 550 | 3300000 | 285 | 296 | 298 | 267 | 286.5 | 1719000 | 52.090909090909086 | Q(+sides) | 23 | 28 | 26 | 30 | 107 | 5 | 102 | 102 | 5.933682373472949E-5 | 59.33682373472949 |
| 19-603 | 300 | 328 | 331 | 309 | 317 | 1902000 | 272 | 191 | 156 | 148 | 191.75 | 1150500 | 60.488958990536275 | P | 1 | 4 | 1 | 0 | 6 | 0 | 6 | 6 | 5.215123859191656E-6 | 5.215123859191656 |
| 19-1167 | 628 | 420 | 620 | 740 | 602 | 3612000 | 302 | 294 | 274 | 292 | 290.5 | 1743000 | 48.25581395348838 | B | 3 | 1 | 2 | 4 | 10 | 1 | 9 | 9 | 5.163511187607573E-6 | 5.163511187607573 |
| 19-775 | 576 | 679 | 650 | 633 | 634.5 | 3807000 | 334 | 265 | 281 | 234 | 278.5 | 1671000 | 43.89282899921198 | A(sides) | 2 | 2 | 1 | 1 | 6 | 1 | 5 | 5 | 2.992220227408737E-6 | 2.9922202274087373 |
| 19-744 | 588 | 612 | 600 | 3600000 | 413 | 466 | 504 | 465 | 462 | 2772000 | 77 | BCDE | 104 | 122 | 77 | 80 | 383 | 13 | 370 | 370 | 1.3347763347763348E-4 | 133.47763347763347 | ||
| 19-1256 | 576 | 564 | 664 | 748 | 638 | 3828000 | 339 | 364 | 326 | 331 | 340 | 2040000 | 53.29153605015674 | K | 20 | 12 | 12 | 14 | 58 | 4 | 54 | 54 | 2.647058823529412E-5 | 26.47058823529412 |
| 19-1248 | 359 | 318 | 291 | 325 | 323.25 | 1939500 | 177 | 174 | 192 | 184 | 181.75 | 1090500 | 56.22583139984532 | A | 4 | 6 | 5 | 3 | 18 | 7 | 11 | 11 | 1.008711600183402E-5 | 10.087116001834021 |
| 19-585 | 171 | 188 | 163 | 194 | 179 | 1074000 | 92 | 102 | 109 | 144 | 111.75 | 670500 | 62.43016759776536 | C | 18 | 12 | 9 | 10 | 49 | 3 | 46 | 46 | 6.86055182699478E-5 | 68.60551826994781 |
| 19-601 | 413 | 298 | 400 | 353 | 366 | 2196000 | 221 | 233 | 219 | 176 | 212.25 | 1273500 | 57.99180327868852 | A | 0 | 0 | 3 | 4 | 7 | 2 | 5 | 5 | 3.926187671770711E-6 | 3.9261876717707107 |
| 19-618 | 342 | 502 | 446 | 364 | 413.5 | 2481000 | 241 | 210 | 256 | 298 | 251.25 | 1507500 | 60.761789600967354 | BC | 34 | 36 | 50 | 40 | 160 | 13 | 147 | 147 | 9.751243781094528E-5 | 97.51243781094527 |
| 19-780 | 414 | 440 | 388 | 420 | 415.5 | 2493000 | 246 | 295 | 258 | 265 | 266 | 1596000 | 64.01925391095065 | A | 0 | 0 | 3 | 2 | 5 | 0 | 5 | 5 | 3.1328320802005014E-6 | 3.1328320802005014 |
| 19-1366 | 166 | 181 | 155 | 139 | 160.25 | 961500 | 94 | 97 | 85 | 70 | 86.5 | 519000 | 53.97815912636506 | D | 13 | 9 | 14 | 10 | 46 | 5 | 41 | 41 | 7.89980732177264E-5 | 78.9980732177264 |
| 19-1150 | 348 | 298 | 195 | 321 | 290.5 | 1743000 | 167 | 157 | 149 | 168 | 160.25 | 961500 | 55.163511187607575 | A | 1 | 4 | 3 | 2 | 10 | 4 | 6 | 6 | 6.2402496099843995E-6 | 6.240249609984399 |
| 19-1392 | 368 | 348 | 412 | 356 | 371 | 2226000 | 245 | 157 | 252 | 230 | 221 | 1326000 | 59.56873315363881 | D | 16 | 9 | 22 | 18 | 65 | 2 | 63 | 63 | 4.751131221719457E-5 | 47.51131221719457 |
| 19-1377 | 422 | 383 | 469 | 452 | 431.5 | 2589000 | 224 | 282 | 293 | 247 | 261.5 | 1569000 | 60.60254924681344 | A | 0 | 1 | 2 | 5 | 8 | 2 | 6 | 6 | 3.824091778202677E-6 | 3.824091778202677 |
| 19-737 | 276 | 250 | 308 | 280 | 278.5 | 1671000 | 138 | 111 | 113 | 104 | 116.5 | 699000 | 41.83123877917414 | EF | 33 | 38 | 35 | 59 | 165 | 10 | 155 | 155 | 2.217453505007153E-4 | 221.74535050071532 |
| 19-804 | 378 | 406 | 439 | 460 | 420.75 | 2524500 | 321 | 264 | 278 | 162 | 256.25 | 1537500 | 60.903149138443254 | A | 1 | 1 | 2 | 3 | 7 | 0 | 7 | 7 | 4.552845528455284E-6 | 4.5528455284552845 |
| 19-610 | 211 | 264 | 256 | 255 | 246.5 | 1479000 | 185 | 205 | 166 | 196 | 188 | 1128000 | 76.26774847870182 | VWX | 68 | 62 | 38 | 68 | 236 | 10 | 226 | 226 | 2.00354609929078E-4 | 200.354609929078 |
| 19-607 | 450 | 495 | 495 | 490 | 482.5 | 2895000 | 313 | 238 | 297 | 349 | 299.25 | 1795500 | 62.020725388601036 | MN | 45 | 51 | 52 | 44 | 192 | 9 | 183 | 183 | 1.0192147034252297E-4 | 101.92147034252298 |
| 19-722 | 243 | 222 | 293 | 290 | 262 | 1572000 | 181 | 188 | 190 | 186.33333333333334 | 1118000 | 71.11959287531806 | A | 8 | 13 | 11 | 7 | 39 | 4 | 35 | 35 | 3.1305903398926656E-5 | 31.305903398926656 | |
| 19-776 | 285 | 280 | 275 | 232 | 268 | 1608000 | 165 | 133 | 102 | 123 | 130.75 | 784500 | 48.78731343283582 | A | 1 | 1 | 1 | 1 | 4 | 1 | 3 | 3 | 3.824091778202677E-6 | 3.824091778202677 |
| 19-1164 | 416 | 436 | 408 | 424 | 421 | 2526000 | 313 | 317 | 332 | 330 | 323 | 1938000 | 76.72209026128266 | F | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 1 | 5.159958720330238E-7 | 0.5159958720330238 |
| 19-555 | 120 | 135 | 189 | 149 | 148.25 | 889500 | 141 | 165 | 114 | 120 | 135 | 810000 | 91.06239460370995 | GH | 47 | 48 | 28 | 31 | 154 | 5 | 149 | 149 | 1.8395061728395062E-4 | 183.95061728395063 |
| 19-734 | 262 | 342 | 310 | 336 | 312.5 | 1875000 | 320 | 376 | 289 | 306 | 322.75 | 1936500 | 103.28 | U | 1 | 0 | 2 | 2 | 5 | 0 | 5 | 5 | 2.581977794990963E-6 | 2.581977794990963 |
| 19-606 | 191 | 220 | 207 | 229 | 211.75 | 1270500 | 264 | 205 | 264 | 195 | 232 | 1392000 | 109.56316410861866 | JK | 54 | 49 | 52 | 46 | 201 | 10 | 191 | 191 | 1.3721264367816093E-4 | 137.21264367816093 |
| 19-779 | 286 | 324 | 254 | 324 | 297 | 1782000 | 237 | 233 | 262 | 215 | 236.75 | 1420500 | 79.71380471380472 | F | 0 | 4 | 1 | 4 | 9 | 1 | 8 | 8 | 5.631819781766984E-6 | 5.631819781766984 |
| 19-1254 | 260 | 276 | 286 | 252 | 268.5 | 1611000 | 211 | 252 | 228 | 240 | 232.75 | 1396500 | 86.6852886405959 | E | 25 | 14 | 23 | 27 | 89 | 3 | 86 | 86 | 6.158252774794128E-5 | 61.58252774794128 |
| 19-1148 | 364 | 284 | 280 | 312 | 310 | 1860000 | 221 | 238 | 186 | 217 | 215.5 | 1293000 | 69.51612903225806 | E + sides | 4 | 2 | 3 | 3 | 12 | 0 | 12 | 12 | 9.280742459396751E-6 | 9.280742459396752 |
| 19-1393 | 180 | 172 | 169 | 173 | 173.5 | 1041000 | 197 | 141 | 237 | 152 | 181.75 | 1090500 | 104.75504322766571 | F | 13 | 28 | 16 | 30 | 87 | 7 | 80 | 80 | 7.336084364970197E-5 | 73.36084364970196 |
| 19-772 | 185 | 187 | 158 | 157 | 171.75 | 1030500 | 148 | 154 | 114 | 125 | 135.25 | 811500 | 78.74818049490538 | F | 1 | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 1.2322858903265557E-6 | 1.2322858903265557 |
| 19-736 | 173 | 156 | 141 | 148 | 154.5 | 927000 | 145 | 153 | 167 | 140 | 151.25 | 907500 | 97.89644012944983 | KLM | 40 | 47 | 48 | 101 | 236 | 10 | 226 | 226 | 2.490358126721763E-4 | 249.0358126721763 |
| 19-805 | 432 | 288 | 452 | 432 | 401 | 2406000 | 300 | 299 | 257 | 273 | 282.25 | 1693500 | 70.38653366583542 | M | 3 | 1 | 3 | 1 | 8 | 1 | 7 | 7 | 4.133451431945675E-6 | 4.133451431945675 |
| 19-609 | 206 | 284 | 281 | 244 | 253.75 | 1522500 | 184 | 229 | 345 | 296 | 263.5 | 1581000 | 103.84236453201972 | HI | 39 | 36 | 39 | 31 | 145 | 9 | 136 | 136 | 8.602150537634408E-5 | 86.02150537634408 |
| 19-1235 | 300 | 360 | 356 | 360 | 344 | 2064000 | 328 | 208 | 263 | 236 | 258.75 | 1552500 | 75.21802325581395 | I | 1 | 2 | 0 | 0 | 3 | 0 | 3 | 3 | 1.932367149758454E-6 | 1.932367149758454 |
| 19-1394 | 178 | 216 | 217 | 221 | 208 | 1248000 | 220 | 221 | 180 | 230 | 212.75 | 1276500 | 102.28365384615385 | N | 33 | 18 | 22 | 16 | 89 | 11 | 78 | 78 | 6.110458284371328E-5 | 61.10458284371328 |
| 19-1378 | 205 | 193 | 187 | 182 | 191.75 | 1150500 | 204 | 170 | 138 | 152 | 166 | 996000 | 86.57105606258149 | U | 2 | 1 | 0 | 0 | 3 | 0 | 3 | 3 | 3.012048192771084E-6 | 3.012048192771084 |
