Optimum pH and stability

Selected Cell

Cell:

Value:

Introduction Results WT 0 h WT 30 min WT 1 h WT 5 h WT 7 h WT 24 h V37A 0 h V37A 30 min V37A 1 h V37A 5 h V37A 7 h V37A 24 h

pH optimum and pH stability

Methodology

The activity/pH profile was determined by incubating the enzyme at room temperature

in 50 mM of KPi pH=8, CHES pH= 9, CHES pH =10. All buffers contained 0.1 mM PLP.

Samples (90 μL) were taken from the reaction mixture at different time points and transfered to a 96-well plate.

The enzyme activity was photometrically determined according to the acetophenone assay (Schätzle et al. 2009).

Enantiopure (R)-phenylethylamine (R-PEA)/DMSO 1mM and Sodium pyruvate 4mM were then added

and the rate was determined by measuring the absorbance (245 nm) of acetophenone that is produced.

One unit of activity was defined as the amount of enzyme that produced 1 µmol acetophenone per minute.

Assay Conditions

Reaction mixture: 100 μL purified enzyme + 1,7 mL buffer

Incubation and measurements were done at room temperature.

Buffers:

Kpi 50 mM/ PLP 0.1 mM pH = 8

CHES 50 mM/ PLP 0.1 mM pH = 9

CHES 50 mM/ PLP 0.1 mM pH = 10

Stock solutions:

R-PEA/DMSO 20 mM, 20 mL

Sodium Pyruvate 4 mM, 50 mL in ddH2O

The assay componets were pipetted in the appropriate well in the following order:

10 μL of amine stock solution (20 mM R-PEA/DMSO)

90 μL reaction mixture

100 μl of pyruvate stock solution (4 mM, ddH2O)


pH Optimum and pH stability


	Optimum pH LS_ATA WT							Optimum pH LS_ATA V37A
	pH	U/mL	stdev					pH	U/mL	stdev
	8	1.3314048669943188	0.031201533091684715					8	0.1114289536681478	0.01492061503272873
	9	1.5575538991185927	0.026531417415365757					9	0.24274988814475817	0.004922148016298904
	10	2.2041703580760963	0.017207578285694723					10	0.3605740034454532	0.007666415320429861






	LS_ATA stability in fifferent pH conditions after 24 h of incubation							LS_ATA V37A stability in fifferent pH conditions after 24 h of incubation
	hours	pH 8		pH 9		pH 10			pH 8		pH 9		pH 10
	0	1.3314048669943188	0.031201533091684715	1.5575538991185927	0.026531417415365757	2.2041703580761	0.017207578285694723	0	0.1114289536681478	0.01492061503272873	0.24274988814475817	0.004922148016298904	0.3605740034454532	0.007666415320429861
	0.5	1.5481321411819355	0.03762497655866835	1.6484519806123485	0.012858079084280926	2.2003597579689385	0.00873034974322726	0.5	0.12496824528704581	0.004912929519516471	0.2588607463923367	0.004869676248052912	0.322916221378135	0.003261562128723631
	1	1.5830603709318567	0.03010759541781213	1.7893757239888275	0.15128364904018068	2.1434755328200272	0.019430243253075897	1	0.12327300709929301	0.002415887221377865	0.25406342473788257	0.004164240332139905	0.3028677441602239	0.004924349821720339
	5	1.7219593847633992	0.009841091265908661	2.0985091800354305	0.14645235299220194	2.210916075063697	0.14558545220083627	5	0.1436369880356361	0.00569779001817054	0.27719708404068455	0.0011270551551225544	0.2648951036278375	0.007441321543483378
	7	1.6139576454636624	0.02801632982826174	1.9374880029939325	0.03897196104685616	2.091170902160943	0.06642819144311542	7	0.15181203827035628	0.003375439196510291	0.2931707005024668	0.0039009098917662326	0.27177555332798004	0.002462264739874457
	24	1.5214517674921195	0.0723811472134384	1.6124868682270455	0	1.560894199391076	0	24	0.12031520569524523	0.0026923029919193237	0.2613694184630786	6.260059100852637E-4	0.27863907073433136	0.0021772799664530216

100

without incubation
LS_ATA WT					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	(mg/ml)	U/mg	STDEV
A1	WT_pH 8	0.005192485592271335	0.9994241879037777	0.3115491355362801	0.03404908585095957	40	1.3619634340383828	1.3314048669943188	0.031201533091684715	4.5	0.29586774822095974	0.006933674020374381
B1	WT_pH 8	0.004954716782514308	0.9998686140371876	0.2972830069508585	0.03248994611484792	40	1.299597844593917
C1	WT_pH 8	0.005080740791461881	0.9977400911775439	0.30484444748771283	0.033316333058766426	40	1.332653322350657

E1	WT_pH 9	0.005964726920392948	0.9995401753248008	0.35788361522357687	0.03911296341241277	40	1.5645185364965108	1.5575538991185927	0.026531417415365757	4.5	0.3461230886930206	0.005895870536747946
F1	WT_pH 9	0.005826395377398884	0.9992934487754209	0.349583722643933	0.03820587132720579	40	1.5282348530882317
G1	WT_pH 9	0.006023400423377074	0.9984026200836985	0.36140402540262445	0.039497707694275894	40	1.5799083077710359

A2	WT_pH 10	0.008430333755856674	0.9980462922367128	0.5058200253514005	0.05528087708758475	40	2.2112350835033903	2.2041703580760963	0.017207578285694723	4.5	0.4898156351280214	0.0038239062857099385
B2	WT_pH 10	0.00832861531505215	0.9994740373299923	0.499716918903129	0.05461387091837475	40	2.18455483673499
C2	WT_pH 10	0.008451249399586528	0.9988476348351235	0.5070749639751917	0.055418028849747725	40	2.216721153989909



Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared		Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared	R squared
A 1	0.8106	0	0.005192485592271335	0.9994241879037777		A 2	0.7746	0.01	0.008430333755856674	0.9980462922367128
A 1	0.8718	10.47				A 2	0.8917	10.49
A 1	0.9326	20.44				A 2	0.9816	20.45
A 1	0.9825	30.4				A 2	1.0659	30.42
A 1	1.0349	40.46				A 2	1.1514	40.48
A 1	1.0913	50.43				A 2	1.2313	50.44
A 1	1.1413	60.39				A 2	1.3135	60.41
A 1	1.194	70.46				A 2	1.3942	70.48
A 1	1.2442	80.43				A 2	1.4718	80.44
A 1	1.2971	90.39				A 2	1.5512	90.41
A 1	1.347	100.46				A 2	1.6291	100.48
A 1	1.3967	110.43				A 2	1.7032	110.44
A 1	1.4471	120.39				A 2	1.7782	120.41
A 1	1.4959	130.46				A 2	1.8506	130.48
A 1	1.5432	140.43				A 2	1.9215	140.44
A 1	1.5913	150.39				A 2	1.9895	150.41
A 1	1.6398	160.46				A 2	2.0588	160.48
A 1	1.6847	170.43				A 2	2.1254	170.44
A 1	1.7304	180.39				A 2	2.1914	180.41
A 1	1.7778	190.46				A 2	2.2562	190.48
A 1	1.8228	200.43				A 2	2.3197	200.44
A 1	1.8651	210.39				A 2	2.3792	210.41
A 1	1.9094	220.46				A 2	2.4375	220.48
A 1	1.9513	230.43				A 2	2.4921	230.44
A 1	1.9943	240.39				A 2	2.5446	240.41
A 1	2.033	250.46				A 2	2.5987	250.48
A 1	2.0761	260.43				A 2	2.648	260.44
A 1	2.1167	270.39				A 2	2.7021	270.41
A 1	2.1547	280.46				A 2	2.7469	280.48
A 1	2.1906	290.43				A 2	2.7822	290.44
A 1	2.2302	300.39				A 2	2.8338	300.41
B 1	0.8249	0.46	0.004954716782514308	0.9998686140371876		B 2	0.7794	0.44	0.00832861531505215	0.9994740373299923
B 1	0.8758	10.93				B 2	0.8725	10.91
B 1	0.9235	20.9				B 2	0.967	20.88
B 1	0.9743	30.86				B 2	1.0459	30.85
B 1	1.0247	40.92				B 2	1.1331	40.9
B 1	1.0758	50.89				B 2	1.2138	50.87
B 1	1.1238	60.85				B 2	1.2949	60.84
B 1	1.175	70.92				B 2	1.3767	70.9
B 1	1.2256	80.89				B 2	1.4555	80.87
B 1	1.276	90.85				B 2	1.5363	90.84
B 1	1.3247	100.92				B 2	1.6115	100.9
B 1	1.3757	110.89				B 2	1.686	110.87
B 1	1.4222	120.85				B 2	1.7609	120.84
B 1	1.469	130.92				B 2	1.8312	130.9
B 1	1.5144	140.89				B 2	1.902	140.87
B 1	1.5596	150.85				B 2	1.9691	150.84
B 1	1.6037	160.92				B 2	2.0364	160.9
B 1	1.6493	170.89				B 2	2.1038	170.87
B 1	1.6915	180.85				B 2	2.1658	180.84
B 1	1.7326	190.92				B 2	2.2272	190.9
B 1	1.7748	200.89				B 2	2.2879	200.87
B 1	1.8173	210.85				B 2	2.3452	210.84
B 1	1.8578	220.92				B 2	2.3974	220.9
B 1	1.8978	230.89				B 2	2.4547	230.87
B 1	1.9368	240.85				B 2	2.5089	240.84
B 1	1.979	250.92				B 2	2.5613	250.9
B 1	2.0154	260.89				B 2	2.6042	260.87
B 1	2.0513	270.85				B 2	2.6528	270.84
B 1	2.0897	280.92				B 2	2.7015	280.9
B 1	2.1247	290.89				B 2	2.7271	290.87
B 1	2.1479	300.85				B 2	2.7726	300.84
C 1	0.836	0.88	0.005080740791461881	0.9977400911775439		C 2	0.7893	0.9	0.008451249399586528	0.9988476348351235
C 1	0.8894	11.36				C 2	0.887	11.38
C 1	0.9419	21.32				C 2	0.9709	21.34
C 1	0.9955	31.29				C 2	1.0614	31.31
C 1	1.0569	41.35				C 2	1.1473	41.37
C 1	1.1156	51.31				C 2	1.24	51.33
C 1	1.1683	61.28				C 2	1.3204	61.3
C 1	1.219	71.35				C 2	1.3992	71.37
C 1	1.262	81.31				C 2	1.4741	81.33
C 1	1.32	91.28				C 2	1.5505	91.3
C 1	1.3643	101.35				C 2	1.6228	101.37
C 1	1.4055	111.31				C 2	1.6934	111.33
C 1	1.4558	121.28				C 2	1.7647	121.3
C 1	1.4978	131.35				C 2	1.8335	131.37
C 1	1.5439	141.31				C 2	1.8987	141.33
C 1	1.5876	151.28				C 2	1.9638	151.3
C 1	1.6334	161.35				C 2	2.0285	161.37
C 1	1.6779	171.31				C 2	2.0904	171.33
C 1	1.7212	181.28				C 2	2.1502	181.3

↑ Previous 1 2 3 Next ↓

100

after 30 min of incubation					mM/min
LS_ATA WT					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A1	WT_pH 8	0.005874082924286031	0.9989560405820507	0.35244497545716186	0.038518576552695284	40	1.5407430621078113	1.5481321411819355	0.03762497655866835
B1	WT_pH 8	0.005774983874193791	0.9985430329844883	0.3464990324516275	0.03786874671602486	40	1.5147498686409944
C1	WT_pH 8	0.00605769456628857	0.9986367632636859	0.36346167397731416	0.03972258731992504	40	1.5889034927970016

D1	WT_pH 9	0.006234785683158333	0.9995453772821179	0.3740871409895	0.040883840545300545	40	1.6353536218120217	1.6484519806123485	0.012858079084280926
E1	WT_pH 9	0.006286609778861714	0.999663576141679	0.37719658673170287	0.04122367068106042	40	1.6489468272424168
F1	WT_pH 9	0.00633277406623369	0.9993546736130912	0.3799664439740214	0.04152638731956518	40	1.6610554927826071

A2	WT_pH 10	0.008351155635963194	0.9996259695830714	0.5010693381577916	0.05476167630139799	40	2.1904670520559195	2.2003597579689385	0.00873034974322726
B2	WT_pH 10	0.008414131578829786	0.9993651280226081	0.5048478947297872	0.05517463330380188	40	2.206985332152075
C2	WT_pH 10	0.008401327516976757	0.9994995425773544	0.5040796510186054	0.05509067224247054	40	2.2036268896988216

G1	blank KPi	-5.5635650758506745E-5	0.605883326598542
H1	blank CHES	-2.7381927580842764E-5	0.674484109315332


Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared		Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared
A 1	0.7977	0	0.005874082924286031	0.9989560405820507		A 2	0.7331	0.01	0.008351155635963194	0.9996259695830714
A 1	0.8509	10.47				A 2	0.8058	10.49
A 1	0.9218	20.44				A 2	0.8935	20.45
A 1	0.9823	30.4				A 2	0.9787	30.42
A 1	1.0454	40.46				A 2	1.0673	40.48
A 1	1.1085	50.43				A 2	1.1538	50.44
A 1	1.1674	60.39				A 2	1.2378	60.41
A 1	1.2257	70.46				A 2	1.3201	70.48
A 1	1.2842	80.43				A 2	1.4008	80.44
A 1	1.3405	90.39				A 2	1.4803	90.41
A 1	1.3956	100.46				A 2	1.5592	100.48
A 1	1.4497	110.43				A 2	1.6339	110.44
A 1	1.5049	120.39				A 2	1.7087	120.41
A 1	1.556	130.46				A 2	1.7823	130.48
A 1	1.6061	140.43				A 2	1.8548	140.44
A 1	1.6542	150.39				A 2	1.9252	150.41
A 1	1.702	160.46				A 2	1.9947	160.48
A 1	1.7517	170.43				A 2	2.0607	170.44
A 1	1.8009	180.39				A 2	2.1262	180.41
A 1	1.851	190.46				A 2	2.1914	190.48
A 1	1.9032	200.43				A 2	2.2557	200.44
A 1	1.9522	210.39				A 2	2.3174	210.41
A 1	1.9988	220.46				A 2	2.3757	220.48
A 1	2.0454	230.43				A 2	2.4331	230.44
A 1	2.0893	240.39				A 2	2.491	240.41
A 1	2.1324	250.46				A 2	2.5454	250.48
A 1	2.1759	260.43				A 2	2.6012	260.44
A 1	2.2181	270.39				A 2	2.651	270.41
A 1	2.2595	280.46				A 2	2.7031	280.48
A 1	2.301	290.43				A 2	2.7546	290.44
A 1	2.3369	300.39				A 2	2.795	300.41
B 1	0.7853	0.46	0.005774983874193791	0.9985430329844883		B 2	0.7381	0.44	0.008414131578829786	0.9993651280226081
B 1	0.8408	10.93				B 2	0.8257	10.91
B 1	0.9028	20.9				B 2	0.9181	20.88
B 1	0.9628	30.86				B 2	1.0055	30.85
B 1	1.023	40.92				B 2	1.0935	40.9
B 1	1.0849	50.89				B 2	1.1779	50.87
B 1	1.1454	60.85				B 2	1.2612	60.84
B 1	1.2072	70.92				B 2	1.3437	70.9
B 1	1.268	80.89				B 2	1.4239	80.87
B 1	1.3225	90.85				B 2	1.5022	90.83
B 1	1.3753	100.92				B 2	1.579	100.9
B 1	1.4272	110.89				B 2	1.6549	110.87
B 1	1.4768	120.85				B 2	1.7283	120.84
B 1	1.5233	130.92				B 2	1.8025	130.9
B 1	1.5661	140.89				B 2	1.8726	140.87
B 1	1.6114	150.85				B 2	1.9449	150.84
B 1	1.6616	160.92				B 2	2.0131	160.9
B 1	1.7115	170.89				B 2	2.0806	170.87
B 1	1.7624	180.85				B 2	2.1458	180.84
B 1	1.8136	190.92				B 2	2.2105	190.9
B 1	1.8631	200.89				B 2	2.2733	200.87
B 1	1.9105	210.85				B 2	2.3354	210.84
B 1	1.9583	220.92				B 2	2.396	220.9
B 1	2.0049	230.89				B 2	2.4549	230.87
B 1	2.0479	240.85				B 2	2.5079	240.84
B 1	2.0912	250.92				B 2	2.5638	250.9
B 1	2.1317	260.89				B 2	2.6207	260.87
B 1	2.1737	270.85				B 2	2.6707	270.84
B 1	2.2153	280.92				B 2	2.72	280.9
B 1	2.255	290.89				B 2	2.7695	290.87
B 1	2.2913	300.85				B 2	2.8136	300.84
C 1	0.7935	0.88	0.00605769456628857	0.9986367632636859		C 2	0.7401	0.9	0.008401327516976757	0.9994995425773544
C 1	0.8498	11.36				C 2	0.8259	11.38
C 1	0.9166	21.32				C 2	0.9148	21.34
C 1	0.9836	31.29				C 2	1.004	31.31
C 1	1.0506	41.35				C 2	1.0926	41.37
C 1	1.1142	51.31				C 2	1.1768	51.33
C 1	1.1781	61.28				C 2	1.2594	61.3
C 1	1.2405	71.35				C 2	1.3416	71.37
C 1	1.2961	81.31				C 2	1.422	81.33
C 1	1.3528	91.28				C 2	1.5	91.3
C 1	1.41	101.35				C 2	1.5798	101.37
C 1	1.4659	111.31				C 2	1.6559	111.33
C 1	1.5217	121.28				C 2	1.7303	121.3
C 1	1.5747	131.35				C 2	1.8055	131.37
C 1	1.6244	141.31				C 2	1.876	141.33
C 1	1.6708	151.28				C 2	1.9441	151.3
C 1	1.7151	161.35				C 2	2.0115	161.37

↑ Previous 1 2 3 Next ↓

100

after 1 hour of incubation					mM/min
LS_ATA WT					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A3	WT_pH 8	0.005931103271249529	0.9989184350562612	0.35586619627497174	0.03889248046721002	40	1.5556992186884009	1.5830603709318567	0.03010759541781213
B3	WT_pH 8	0.006158388167575014	0.9986923244913039	0.36950329005450083	0.04038287323000009	40	1.6153149292000035
C3	WT_pH 8	0.006016761553708571	0.998787028482415	0.36100569322251425	0.03945417412267915	40	1.5781669649071661

D3	WT_pH 9	0.006459998250311702	0.9991314783358707	0.3875998950187021	0.042360644264339026	40	1.694425770573561	1.7893757239888275	0.15128364904018068
E3	WT_pH 9	0.006518864004670044	0.9995283796768599	0.39113184028020265	0.04274664921095111	40	1.7098659684380444
F3	WT_pH 9	0.007487122588140467	0.9991420113433326	0.449227355288428	0.049095885823871914	40	1.9638354329548766

A4	WT_pH 10	0.008102029148903149	0.9994258337398851	0.4861217489341889	0.05312805999280753	40	2.125122399712301	2.1434755328200272	0.019430243253075897
B4	WT_pH 10	0.008249595184175968	0.9993506859583005	0.4949757110505581	0.054095706125744054	40	2.1638282450297623
C4	WT_pH 10	0.008164377073549943	0.9995859745340296	0.48986262441299655	0.05353689884295044	40	2.1414759537180177

G3	blank KPi	-1.0221660985651791E-6	0.25369874393014913
H3	blank CHES	1.22405227751189E-5	0.7047905071299414



Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared		Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared
A 3	0.783	0	0.005931103271249529	0.9989184350562612		A 4	0.7359	0.02	0.008102029148903149	0.9994258337398851
A 3	0.8453	10.42				A 4	0.8127	10.44
A 3	0.909	20.34				A 4	0.8995	20.36
A 3	0.9744	30.35				A 4	0.9874	30.37
A 3	1.0374	40.36				A 4	1.0714	40.37
A 3	1.0991	50.36				A 4	1.1529	50.38
A 3	1.1609	60.37				A 4	1.2341	60.39
A 3	1.2196	70.38				A 4	1.3144	70.4
A 3	1.2782	80.39				A 4	1.3899	80.4
A 3	1.3351	90.39				A 4	1.466	90.41
A 3	1.3911	100.4				A 4	1.5415	100.42
A 3	1.4461	110.41				A 4	1.6152	110.43
A 3	1.4996	120.42				A 4	1.6858	120.43
A 3	1.5517	130.42				A 4	1.7555	130.44
A 3	1.603	140.34				A 4	1.8235	140.36
A 3	1.6498	150.35				A 4	1.8896	150.37
A 3	1.7007	160.36				A 4	1.9564	160.37
A 3	1.7488	170.36				A 4	2.0204	170.38
A 3	1.7966	180.37				A 4	2.0857	180.39
A 3	1.8438	190.38				A 4	2.1477	190.4
A 3	1.8888	200.39				A 4	2.2078	200.4
A 3	1.9321	210.39				A 4	2.2653	210.41
A 3	1.978	220.4				A 4	2.3231	220.42
A 3	2.0201	230.41				A 4	2.3765	230.43
A 3	2.0618	240.42				A 4	2.4307	240.43
A 3	2.1035	250.42				A 4	2.487	250.44
A 3	2.1466	260.34				A 4	2.5384	260.36
A 3	2.1853	270.35				A 4	2.5934	270.37
A 3	2.2254	280.36				A 4	2.6366	280.37
A 3	2.2632	290.36				A 4	2.685	290.38
A 3	2.3009	300.37				A 4	2.7296	300.39
B 3	0.8062	0.46	0.006158388167575014	0.9986923244913039		B 4	0.7422	0.44	0.008249595184175968	0.9993506859583005
B 3	0.8737	10.89				B 4	0.8328	10.87
B 3	0.9425	20.8				B 4	0.9195	20.78
B 3	1.0068	30.81				B 4	1.0066	30.79
B 3	1.0732	40.82				B 4	1.0925	40.8
B 3	1.1389	50.82				B 4	1.1754	50.81
B 3	1.2026	60.83				B 4	1.2575	60.81
B 3	1.2644	70.84				B 4	1.3374	70.82
B 3	1.3238	80.85				B 4	1.4161	80.83
B 3	1.3828	90.85				B 4	1.4931	90.84
B 3	1.4408	100.86				B 4	1.569	100.84
B 3	1.4966	110.87				B 4	1.6426	110.85
B 3	1.5523	120.88				B 4	1.7169	120.86
B 3	1.6067	130.88				B 4	1.788	130.87
B 3	1.6587	140.8				B 4	1.8569	140.78
B 3	1.7106	150.81				B 4	1.9263	150.79
B 3	1.7611	160.82				B 4	1.9926	160.8
B 3	1.8097	170.82				B 4	2.0575	170.81
B 3	1.8573	180.83				B 4	2.1211	180.81
B 3	1.9065	190.84				B 4	2.183	190.82
B 3	1.9526	200.85				B 4	2.2447	200.83
B 3	1.9986	210.85				B 4	2.3041	210.84
B 3	2.0445	220.86				B 4	2.3617	220.84
B 3	2.0875	230.87				B 4	2.4206	230.85
B 3	2.1301	240.88				B 4	2.4764	240.86
B 3	2.1743	250.88				B 4	2.5297	250.87
B 3	2.2148	260.8				B 4	2.5861	260.78
B 3	2.253	270.81				B 4	2.628	270.79
B 3	2.2936	280.82				B 4	2.6806	280.8
B 3	2.3353	290.82				B 4	2.7281	290.81
B 3	2.3681	300.83				B 4	2.7747	300.81
C 3	0.7979	0.89	0.006016761553708571	0.998787028482415		C 4	0.7332	0.9	0.008164377073549943	0.9995859745340296
C 3	0.8571	11.31				C 4	0.817	11.33
C 3	0.9206	21.23				C 4	0.903	21.25
C 3	0.9892	31.23				C 4	0.9892	31.25
C 3	1.0542	41.24				C 4	1.0733	41.26
C 3	1.1172	51.25				C 4	1.1557	51.27
C 3	1.1791	61.26				C 4	1.2373	61.28
C 3	1.2396	71.26				C 4	1.3173	71.28
C 3	1.298	81.27				C 4	1.3953	81.29
C 3	1.3562	91.28				C 4	1.473	91.3
C 3	1.4126	101.29				C 4	1.5486	101.31
C 3	1.4679	111.29				C 4	1.6233	111.31
C 3	1.5216	121.3				C 4	1.6958	121.32
C 3	1.5751	131.31				C 4	1.7672	131.33
C 3	1.627	141.23				C 4	1.8373	141.24
C 3	1.6778	151.23				C 4	1.9051	151.25

↑ Previous 1 2 3 Next ↓

100

after 5 hours of incubation					mM/min
LS_ATA WT					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A7	WT_pH 8	0.006588172442613334	0.9989584353008215	0.3952903465568	0.04320113077123497	40	1.7280452308493988	1.7219593847633992	0.009841091265908661
B7	WT_pH 8	0.006521684191111468	0.9985985017395845	0.39130105146668803	0.042765142236796505	40	1.7106056894718602
C7	WT_pH 8	0.006585053829506578	0.9990386492335683	0.39510322977039464	0.043180680849223455	40	1.727227233968938

D7	WT_pH 9	0.007661572347766661	0.9988779666108772	0.4596943408659997	0.05023981867387975	40	2.0095927469551897	2.0985091800354305	0.14645235299220194
E7	WT_pH 9	0.007695124653562645	0.9987791066630566	0.4617074792137587	0.05045983379385341	40	2.0183933517541366
F7	WT_pH 9	0.008645001745325932	0.9994239865060143	0.518700104719556	0.056688536034924145	40	2.2675414413969657

A8	WT_pH 10	0.008603639356169611	0.9996183951736388	0.5162183613701766	0.05641730725357121	40	2.2566922901428486	2.210916075063697	0.14558545220083627
B8	WT_pH 10	0.008875926882122739	0.9994372452084582	0.5325556129273643	0.05820279922703435	40	2.328111969081374
C8	WT_pH 10	0.007807786370248687	0.9995692883929207	0.46846718221492123	0.051198599149171714	40	2.0479439659668683

G7	blank KPi	-9.923111899226703E-5	0.6203400409312654
H7	blank CHES	-6.561307259557142E-5	0.46525010307669223


Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared
A 7	0.904	0	0.006588172442613334	0.9989584353008215		A 8	0.8785	0.02	0.008603639356169611	0.9996183951736388
A 7	0.98	10.36				A 8	0.9734	10.38
A 7	1.0481	20.35				A 8	1.0616	20.37
A 7	1.1215	30.34				A 8	1.1503	30.36
A 7	1.192	40.33				A 8	1.2382	40.35
A 7	1.2555	50.32				A 8	1.3227	50.34
A 7	1.3171	60.31				A 8	1.4047	60.33
A 7	1.3823	70.3				A 8	1.4897	70.32
A 7	1.4415	80.29				A 8	1.569	80.31
A 7	1.5064	90.28				A 8	1.6508	90.3
A 7	1.5678	100.27				A 8	1.7274	100.29
A 7	1.6262	110.35				A 8	1.8043	110.37
A 7	1.6836	120.34				A 8	1.8785	120.36
A 7	1.7415	130.33				A 8	1.9523	130.35
A 7	1.7931	140.32				A 8	2.0241	140.34
A 7	1.8437	150.31				A 8	2.0892	150.33
A 7	1.8982	160.3				A 8	2.1609	160.32
A 7	1.9481	170.29				A 8	2.2271	170.31
A 7	1.9991	180.28				A 8	2.2939	180.3
A 7	2.0497	190.27				A 8	2.3594	190.29
A 7	2.0956	200.35				A 8	2.4217	200.37
A 7	2.1428	210.34				A 8	2.4837	210.36
A 7	2.1919	220.33				A 8	2.5433	220.35
A 7	2.2349	230.32				A 8	2.5961	230.34
A 7	2.2786	240.31				A 8	2.6548	240.33
A 7	2.3199	250.3				A 8	2.7051	250.32
A 7	2.3642	260.29				A 8	2.7541	260.31
A 7	2.4018	270.28				A 8	2.8082	270.3
A 7	2.4441	280.27				A 8	2.8532	280.29
A 7	2.4833	290.35				A 8	2.8995	290.37
A 7	2.5185	300.34				A 8	2.9487	300.36
B 7	0.8932	0.46	0.006521684191111468	0.9985985017395845		B 8	0.8728	0.45	0.008875926882122739	0.9994372452084582
B 7	0.9653	10.82				B 8	0.9707	10.8
B 7	1.039	20.81				B 8	1.0652	20.79
B 7	1.1088	30.8				B 8	1.1563	30.78
B 7	1.1774	40.79				B 8	1.245	40.77
B 7	1.2421	50.78				B 8	1.3327	50.76
B 7	1.306	60.77				B 8	1.4197	60.75
B 7	1.3686	70.76				B 8	1.503	70.74
B 7	1.4289	80.75				B 8	1.5854	80.73
B 7	1.4884	90.74				B 8	1.6668	90.72
B 7	1.5468	100.73				B 8	1.7467	100.71
B 7	1.604	110.81				B 8	1.8244	110.79
B 7	1.6602	120.8				B 8	1.8999	120.78
B 7	1.7138	130.79				B 8	1.9738	130.77
B 7	1.7662	140.78				B 8	2.0447	140.76
B 7	1.8176	150.77				B 8	2.1171	150.75
B 7	1.8695	160.76				B 8	2.1859	160.74
B 7	1.9189	170.75				B 8	2.2535	170.73
B 7	1.9698	180.74				B 8	2.3202	180.72
B 7	2.0164	190.73				B 8	2.3824	190.71
B 7	2.0634	200.81				B 8	2.4443	200.79
B 7	2.1096	210.8				B 8	2.5046	210.78
B 7	2.1554	220.79				B 8	2.5652	220.77
B 7	2.2005	230.78				B 8	2.6218	230.76
B 7	2.2431	240.77				B 8	2.6794	240.75
B 7	2.2859	250.76				B 8	2.7308	250.74
B 7	2.3303	260.75				B 8	2.7803	260.73
B 7	2.3664	270.74				B 8	2.8375	270.72
B 7	2.4087	280.73				B 8	2.8838	280.71
B 7	2.4469	290.81				B 8	2.9248	290.79
B 7	2.4853	300.8				B 8	2.975	300.78
C 7	0.8931	0.89	0.006585053829506578	0.9990386492335683		C 8	0.8396	0.91	0.007807786370248687	0.9995692883929207
C 7	0.9608	11.24				C 8	0.9205	11.26
C 7	1.0338	21.23				C 8	1.0034	21.25
C 7	1.1049	31.22				C 8	1.0849	31.24
C 7	1.1735	41.21				C 8	1.1652	41.23
C 7	1.2394	51.2				C 8	1.2427	51.22
C 7	1.3042	61.19				C 8	1.3203	61.21
C 7	1.3677	71.18				C 8	1.3948	71.2
C 7	1.43	81.17				C 8	1.4684	81.19
C 7	1.4913	91.16				C 8	1.5417	91.18
C 7	1.5493	101.15				C 8	1.6112	101.17
C 7	1.6069	111.24				C 8	1.6812	111.25
C 7	1.663	121.23				C 8	1.7497	121.24
C 7	1.7181	131.21				C 8	1.8173	131.23
C 7	1.7717	141.2				C 8	1.8816	141.22
C 7	1.8232	151.19				C 8	1.9453	151.21
C 7	1.8749	161.18				C 8	2.0076	161.2

↑ Previous 1 2 3 Next ↓

100

after 7 hours of incubation					mM/min
LS_ATA WT					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A9	WT_pH 8	0.006031235295216551	0.9989829065488088	0.36187411771299305	0.03954908390305935	40	1.5819633561223738	1.6139576454636624	0.02801632982826174
B9	WT_pH 8	0.006230009036267844	0.9996290894941169	0.3738005421760706	0.04085251827060881	40	1.6341007308243525
C9	WT_pH 8	0.006198396238506245	0.9996356074195468	0.3719037743103747	0.040645221236106525	40	1.625808849444261

D9	WT_pH 9	0.007221446655578062	0.999546952653452	0.4332867993346837	0.04735374856116762	40	1.8941499424467048	1.9374880029939325	0.03897196104685616
E9	WT_pH 9	0.007429268897541852	0.9994305318936676	0.4457561338525111	0.048716517360930175	40	1.9486606944372071
F9	WT_pH 9	0.007509303481123189	0.9994366549374818	0.45055820886739134	0.04924133430244714	40	1.9696533720978857

A10	WT_pH 10	0.008156980836948298	0.9997533285177569	0.4894188502168979	0.05348839893080851	40	2.1395359572323405	2.091170902160943	0.06642819144311542
B10	WT_pH 10	0.007683824852223077	0.9996876462461527	0.46102949113338465	0.05038573673588903	40	2.0154294694355612
C10	WT_pH 10	0.008076961504294409	0.9995737553036018	0.48461769025766455	0.05296368199537317	40	2.1185472798149267

G9	blank KPi	-4.4946334926185954E-5	0.26696181921448003
H9	blank CHES	-4.585687950346681E-5	0.35798647443432274


Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared		Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared
A 9	0.9033	0	0.006031235295216551	0.9989829065488088		A 10	0.8643	0.01	0.008156980836948298	0.9997533285177569
A 9	0.9381	10.32				A 10	0.9413	10.34
A 9	1.0037	20.26				A 10	1.0275	20.28
A 9	1.0668	30.29				A 10	1.1115	30.31
A 9	1.1291	40.31				A 10	1.1945	40.33
A 9	1.192	50.25				A 10	1.2769	50.27
A 9	1.2554	60.28				A 10	1.3577	60.3
A 9	1.3162	70.3				A 10	1.436	70.32
A 9	1.3762	80.24				A 10	1.5123	80.26
A 9	1.4351	90.27				A 10	1.5891	90.29
A 9	1.492	100.29				A 10	1.665	100.31
A 9	1.5475	110.23				A 10	1.7361	110.25
A 9	1.6056	120.26				A 10	1.8093	120.28
A 9	1.6588	130.28				A 10	1.8818	130.3
A 9	1.7116	140.31				A 10	1.9503	140.33
A 9	1.7653	150.25				A 10	2.0183	150.27
A 9	1.8155	160.27				A 10	2.0842	160.29
A 9	1.8668	170.3				A 10	2.1496	170.32
A 9	1.9174	180.24				A 10	2.2155	180.26
A 9	1.9656	190.26				A 10	2.2774	190.28
A 9	2.0128	200.29				A 10	2.3378	200.31
A 9	2.0604	210.23				A 10	2.3987	210.25
A 9	2.105	220.25				A 10	2.4561	220.27
A 9	2.1513	230.28				A 10	2.5106	230.3
A 9	2.195	240.31				A 10	2.5715	240.33
A 9	2.2378	250.25				A 10	2.6213	250.27
A 9	2.2813	260.27				A 10	2.6723	260.29
A 9	2.3235	270.3				A 10	2.7233	270.32
A 9	2.3673	280.24				A 10	2.768	280.26
A 9	2.4043	290.26				A 10	2.8209	290.28
A 9	2.4422	300.29				A 10	2.8666	300.31
B 9	0.8912	0.46	0.006230009036267844	0.9996290894941169		B 10	0.8505	0.44	0.007683824852223077	0.9996876462461527
B 9	0.9538	10.79				B 10	0.9255	10.77
B 9	1.0181	20.72				B 10	1.0069	20.7
B 9	1.0843	30.75				B 10	1.0862	30.73
B 9	1.1496	40.78				B 10	1.1645	40.76
B 9	1.2125	50.71				B 10	1.2435	50.69
B 9	1.2742	60.74				B 10	1.3157	60.72
B 9	1.3344	70.77				B 10	1.39	70.75
B 9	1.3934	80.7				B 10	1.4627	80.68
B 9	1.4527	90.73				B 10	1.5342	90.71
B 9	1.5104	100.76				B 10	1.6048	100.74
B 9	1.5837	110.69				B 10	1.6742	110.67
B 9	1.6448	120.72				B 10	1.7421	120.7
B 9	1.6942	130.75				B 10	1.8093	130.73
B 9	1.7449	140.77				B 10	1.8742	140.76
B 9	1.7953	150.71				B 10	1.8995	150.69
B 9	1.8497	160.74				B 10	2.0274	160.72
B 9	1.8995	170.76				B 10	2.1029	170.74
B 9	1.9486	180.7				B 10	2.16	180.68
B 9	1.9975	190.73				B 10	2.2241	190.71
B 9	2.0457	200.75				B 10	2.2858	200.74
B 9	2.092	210.69				B 10	2.3464	210.67
B 9	2.1366	220.72				B 10	2.405	220.7
B 9	2.1819	230.74				B 10	2.4641	230.73
B 9	2.2253	240.77				B 10	2.5201	240.75
B 9	2.2681	250.71				B 10	2.5736	250.69
B 9	2.3093	260.74				B 10	2.6236	260.72
B 9	2.3513	270.76				B 10	2.6782	270.75
B 9	2.3863	280.7				B 10	2.7243	280.68
B 9	2.429	290.73				B 10	2.7728	290.71
B 9	2.4657	300.75				B 10	2.8142	300.74
C 9	0.9041	0.88	0.006198396238506245	0.9996356074195468		C 10	0.8795	0.9	0.008076961504294409	0.9995737553036018
C 9	0.9635	11.21				C 10	0.9483	11.23
C 9	1.0287	21.15				C 10	1.0329	21.17
C 9	1.0939	31.18				C 10	1.1175	31.19
C 9	1.1585	41.2				C 10	1.2006	41.22
C 9	1.222	51.14				C 10	1.2835	51.16
C 9	1.2843	61.16				C 10	1.3624	61.18
C 9	1.3459	71.19				C 10	1.4406	71.21
C 9	1.4061	81.13				C 10	1.5175	81.15
C 9	1.4656	91.15				C 10	1.5932	91.17
C 9	1.5242	101.18				C 10	1.6696	101.2
C 9	1.5814	111.12				C 10	1.742	111.14
C 9	1.6369	121.14				C 10	1.8151	121.16
C 9	1.6918	131.17				C 10	1.8858	131.19
C 9	1.7444	141.2				C 10	1.9545	141.22
C 9	1.796	151.13				C 10	2.0229	151.15
C 9	1.8478	161.16				C 10	2.0895	161.18

↑ Previous 1 2 3 Next ↓

100

after 24 hours of incubation					mM/min
LS_ATA WT					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A11	WT_pH 8	0.005605406538469597	0.9995941644718326	0.33632439230817585	0.036756764186685886	40	1.4702705674674355	1.5214517674921195	0.0723811472134384
B11	WT_pH 8	0.005995663188657813	0.9993468152817544	0.3597397913194688	0.03931582418792009	40	1.5726329675168036
	WT_pH 8
C11		0.006147606185115611	0.9994128895591302	0.36885637110693664	0.040312171705676136	40	1.6124868682270455	1.6124868682270455
D11	WT_pH 9	0.0030316565516571043	0.99821165023035	πηρα λιγοτερα από 90 μL, δεν μου εφτασε
	WT_pH 9
E11	WT_pH 9	0.005950909135178478	0.9995630614195287	0.35705454811070864	0.0390223549847769	40	1.560894199391076	1.560894199391076
F11		0.003837696318013859	0.9994251512029336	πηρα λιγοτερα από 90 μL, δεν μου εφτασε
	WT_pH 10
G11	WT_pH 10	-7.112293418133706E-5	0.7501336324385042
H11	WT_pH 10	-1.6573052154642316E-4	0.797280475562997

	blank KPi
Well	blank CHES	Measurement time(s)	SLOPE sample	R squared
A 11	0.7678	0	0.005605406538469597	0.9995941644718326
A 11	0.8141	10.29
A 11	0.8722	20.21
A 11	0.9288	30.21
A 11	0.9871	40.22
A 11	1.0461	50.23
A 11	1.1047	60.24
A 11	1.1621	70.25
A 11	1.2193	80.25
A 11	1.2743	90.26
A 11	1.3314	100.27
A 11	1.3846	110.28
A 11	1.4372	120.28
A 11	1.4891	130.29
A 11	1.5401	140.21
A 11	1.5885	150.22
A 11	1.6371	160.22
A 11	1.6835	170.23
A 11	1.7315	180.24
A 11	1.7754	190.25
A 11	1.8212	200.26
A 11	1.8692	210.26
A 11	1.9089	220.27
A 11	1.9451	230.28
A 11	1.977	240.29
A 11	2.0185	250.2
A 11	2.0582	260.21
A 11	2.0992	270.22
A 11	2.1444	280.23
A 11	2.1871	290.23
A 11	2.2271	300.24
B 11	0.7767	0.43	0.005995663188657813	0.9993468152817544
B 11	0.8387	10.72
B 11	0.9011	20.63
B 11	0.9704	30.64
B 11	1.0333	40.65
B 11	1.0923	50.65
B 11	1.1527	60.66
B 11	1.2127	70.67
B 11	1.2739	80.68
B 11	1.3331	90.69
B 11	1.3911	100.69
B 11	1.4487	110.7
B 11	1.5059	120.71
B 11	1.5604	130.72
B 11	1.6135	140.63
B 11	1.6659	150.64
B 11	1.719	160.65
B 11	1.7698	170.66
B 11	1.8197	180.66
B 11	1.8694	190.67
B 11	1.9183	200.68
B 11	1.9649	210.69
B 11	2.0105	220.7
B 11	2.0555	230.7
B 11	2.1001	240.71
B 11	2.1438	250.63
B 11	2.1856	260.63
B 11	2.2283	270.64
B 11	2.2698	280.65
B 11	2.3083	290.66
B 11	2.3463	300.67
C 11	0.793	0.85	0.006147606185115611	0.9994128895591302
C 11	0.8478	11.14
C 11	0.9134	21.06
C 11	0.9798	31.06
C 11	1.0448	41.07
C 11	1.1086	51.08
C 11	1.172	61.09
C 11	1.2349	71.09
C 11	1.2959	81.1
C 11	1.3563	91.11
C 11	1.4169	101.12
C 11	1.4753	111.13
C 11	1.5332	121.13
C 11	1.59	131.14
C 11	1.6441	141.06
C 11	1.6984	151.07
C 11	1.7524	161.07
C 11	1.8049	171.08
C 11	1.8553	181.09
C 11	1.9077	191.1

↑ Previous 1 2 3 Next ↓

100

without incubation					mM/min
LS_ATA V37A					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A1	V37A_pH 8	3.7802294103051625E-4	0.9281974097938879	0.022681376461830976	0.0024788389575771557	40	0.09915355830308623	0.1114289536681478	0.01492061503272873	0.671450084152715	0.1659526989392771	0.02222148062064309
B1	V37A_pH 8	4.0830783049922365E-4	0.978349741132676	0.02449846982995342	0.0026774283967162206	40	0.10709713586864883
C1	V37A_pH 8	4.8813788604970044E-4	0.9977670309240102	0.029288273162982027	0.0032009041708177077	40	0.1280361668327083

D1	V37A_pH 9	9.092244731973357E-4	0.99869825792155	0.054553468391840144	0.005962127693097283	40	0.2384851077238913	0.24274988814475817	0.004922148016298904	0.671450084152715	0.36153080306941626	0.007330623872822999
E1	V37A_pH 9	9.212091229053054E-4	0.9992029639900936	0.055272547374318325	0.00604071556003479	40	0.2416286224013916
F1	V37A_pH 9	9.460182495530307E-4	0.9992576668353714	0.05676109497318184	0.0062033983577247905	40	0.2481359343089916

A2	V37A_pH 10	0.0013418139175236281	0.9974214469100742	0.08050883505141769	0.008798779787040184	40	0.35195119148160736	0.3605740034454532	0.007666415320429861	0.671450084152715	0.5370079056590662	0.011417699545162625
B2	V37A_pH 10	0.0013977392497758591	0.9972385438680185	0.08386435498655155	0.009165503277218748	40	0.3666201310887499
C2	V37A_pH 10	0.001384511997107884	0.9972005774235188	0.08307071982647304	0.009078767194150059	40	0.36315068776600234

G1	blank KPi	-1.0439045770522653E-5	0.3107360292104848
H1	blank CHES	1.004693218296248E-6	0.025703502394261998


Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared	Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared
A 1	0.6803	0	3.7802294103051625E-4	0.9281974097938879	A 2	0.6506	0.02	0.0013418139175236281	0.9974214469100742
A 1	0.6915	10.47			A 2	0.6584	10.49
A 1	0.6973	20.44			A 2	0.6755	20.45
A 1	0.7004	30.4			A 2	0.6903	30.42
A 1	0.7044	40.47			A 2	0.7057	40.49
A 1	0.7082	50.44			A 2	0.7226	50.45
A 1	0.7156	60.4			A 2	0.7376	60.42
A 1	0.7223	70.47			A 2	0.7534	70.49
A 1	0.7305	80.44			A 2	0.768	80.45
A 1	0.7378	90.4			A 2	0.7834	90.42
A 1	0.7435	100.47			A 2	0.7983	100.49
A 1	0.7484	110.44			A 2	0.8125	110.45
A 1	0.7532	120.4			A 2	0.8268	120.42
A 1	0.7571	130.47			A 2	0.8411	130.49
A 1	0.7599	140.44			A 2	0.8539	140.45
A 1	0.7623	150.4			A 2	0.8677	150.42
A 1	0.7621	160.47			A 2	0.8804	160.49
A 1	0.7576	170.44			A 2	0.8923	170.45
A 1	0.7512	180.4			A 2	0.9047	180.42
A 1	0.7485	190.47			A 2	0.9165	190.49
A 1	0.7528	200.44			A 2	0.9287	200.45
A 1	0.7594	210.4			A 2	0.9405	210.42
A 1	0.7672	220.47			A 2	0.9529	220.49
A 1	0.7762	230.44			A 2	0.9661	230.45
A 1	0.7838	240.4			A 2	0.9787	240.42
A 1	0.7893	250.47			A 2	0.9904	250.49
A 1	0.795	260.44			A 2	1.0033	260.45
A 1	0.8011	270.4			A 2	1.0165	270.42
A 1	0.8053	280.47			A 2	1.029	280.49
A 1	0.8106	290.44			A 2	1.0405	290.45
A 1	0.8158	300.4			A 2	1.0534	300.42
B 1	0.6964	0.46	4.0830783049922365E-4	0.978349741132676	B 2	0.6733	0.44	0.0013977392497758591	0.9972385438680185
B 1	0.6894	10.93			B 2	0.6833	10.91
B 1	0.6939	20.9			B 2	0.6987	20.88
B 1	0.6997	30.86			B 2	0.7172	30.85
B 1	0.7039	40.93			B 2	0.7338	40.91
B 1	0.7071	50.9			B 2	0.7505	50.88
B 1	0.7104	60.86			B 2	0.7665	60.85
B 1	0.7161	70.93			B 2	0.7824	70.91
B 1	0.7219	80.9			B 2	0.7984	80.88
B 1	0.7272	90.86			B 2	0.8137	90.85
B 1	0.7323	100.93			B 2	0.8285	100.91
B 1	0.7381	110.9			B 2	0.8437	110.88
B 1	0.7437	120.86			B 2	0.8581	120.85
B 1	0.7494	130.93			B 2	0.8729	130.91
B 1	0.7545	140.9			B 2	0.8864	140.88
B 1	0.7588	150.86			B 2	0.8998	150.85
B 1	0.7629	160.93			B 2	0.9139	160.91
B 1	0.7679	170.9			B 2	0.9272	170.88
B 1	0.7721	180.86			B 2	0.9397	180.85
B 1	0.7757	190.93			B 2	0.9528	190.92
B 1	0.7794	200.9			B 2	0.9664	200.88
B 1	0.7822	210.86			B 2	0.9796	210.85
B 1	0.7829	220.93			B 2	0.9931	220.91
B 1	0.7832	230.9			B 2	1.0051	230.88
B 1	0.7842	240.86			B 2	1.0182	240.85
B 1	0.7858	250.93			B 2	1.0312	250.91
B 1	0.7888	260.9			B 2	1.0435	260.88
B 1	0.7935	270.86			B 2	1.0562	270.85
B 1	0.7989	280.93			B 2	1.068	280.91
B 1	0.8054	290.9			B 2	1.0801	290.88
B 1	0.8118	300.86			B 2	1.0909	300.85
C 1	0.6778	0.89	4.8813788604970044E-4	0.9977670309240102	C 2	0.6576	0.91	0.001384511997107884	0.9972005774235188
C 1	0.6745	11.36			C 2	0.667	11.38
C 1	0.6765	21.32			C 2	0.6839	21.34
C 1	0.683	31.29			C 2	0.7005	31.31
C 1	0.688	41.36			C 2	0.7161	41.38
C 1	0.6924	51.32			C 2	0.7316	51.34
C 1	0.6933	61.29			C 2	0.7476	61.31
C 1	0.6959	71.36			C 2	0.763	71.38
C 1	0.7007	81.32			C 2	0.7783	81.34
C 1	0.7055	91.29			C 2	0.7934	91.31
C 1	0.7112	101.36			C 2	0.8091	101.38
C 1	0.7173	111.32			C 2	0.8241	111.34
C 1	0.7224	121.29			C 2	0.8385	121.31
C 1	0.7291	131.36			C 2	0.8531	131.38
C 1	0.734	141.32			C 2	0.8674	141.34
C 1	0.7397	151.29			C 2	0.8815	151.31
C 1	0.7445	161.36			C 2	0.8949	161.38

↑ Previous 1 2 3 Next ↓

100

after 30 min of incubation					mM/min
LS_ATA V37A					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A3	V37A_pH 8	4.819158146670567E-4	0.9931573233712028	0.028914948880023404	0.0031601037027347983	40	0.12640414810939193	0.12496824528704581	0.004912929519516471
B3	V37A_pH 8	4.918248592854368E-4	0.9966977231001345	0.02950949155712621	0.0032250810444946674	40	0.1290032417797867
C3	V37A_pH 8	4.5558363151809283E-4	0.9802658025224887	0.02733501789108557	0.002987433649298969	40	0.11949734597195877
				0	0	40	0
D3	V37A_pH 9	9.678181428834176E-4	0.9993678758292016	0.05806908857300506	0.00634634847792405	40	0.253853939116962	0.2588607463923367	0.004869676248052912
E3	V37A_pH 9	0.0010049010434961436	0.9997044321213708	0.06029406260976862	0.006589515039318975	40	0.263580601572759
F3	V37A_pH 9	9.880006004827897E-4	0.9994486221255379	0.059280036028967385	0.006478692462182228	40	0.25914769848728914
				0	0	40	0
A4	V37A_pH 10	0.0012167726660533553	0.9978383919285281	0.07300635996320132	0.007978837154448231	40	0.31915348617792927	0.322916221378135	0.003261562128723631
B4	V37A_pH 10	0.0012388184692650398	0.9981093830630642	0.0743291081559024	0.008123399798459278	40	0.3249359919383711
C4	V37A_pH 10	0.0012377631466940243	0.999152834584321	0.07426578880164146	0.008116479650452618	40	0.32465918601810473

G3	blank KPi	-2.6761340046715796E-6	0.130970654356451
H3	blank CHES	-7.430541421045292E-6	0.5440613238007445


Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared	Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared
A 3	0.6642	0	4.819158146670567E-4	0.9931573233712028	A 4	0.6442	0.02	0.0012167726660533553	0.9978383919285281
A 3	0.6594	10.43			A 4	0.6504	10.45
A 3	0.6621	20.35			A 4	0.6655	20.37
A 3	0.6668	30.36			A 4	0.6807	30.38
A 3	0.6752	40.37			A 4	0.6946	40.38
A 3	0.6808	50.37			A 4	0.7089	50.39
A 3	0.6868	60.38			A 4	0.7227	60.4
A 3	0.6904	70.39			A 4	0.7354	70.41
A 3	0.6962	80.4			A 4	0.7496	80.41
A 3	0.7017	90.4			A 4	0.763	90.42
A 3	0.707	100.41			A 4	0.7759	100.43
A 3	0.7125	110.42			A 4	0.7894	110.44
A 3	0.7177	120.43			A 4	0.8013	120.44
A 3	0.7227	130.43			A 4	0.8137	130.45
A 3	0.7284	140.35			A 4	0.8269	140.37
A 3	0.7327	150.36			A 4	0.8381	150.38
A 3	0.7386	160.37			A 4	0.8502	160.38
A 3	0.7433	170.37			A 4	0.8622	170.39
A 3	0.7485	180.38			A 4	0.8743	180.4
A 3	0.7527	190.39			A 4	0.8838	190.41
A 3	0.758	200.4			A 4	0.8958	200.41
A 3	0.763	210.4			A 4	0.9068	210.42
A 3	0.7667	220.41			A 4	0.9189	220.43
A 3	0.7716	230.42			A 4	0.9301	230.44
A 3	0.7756	240.43			A 4	0.9416	240.44
A 3	0.7783	250.43			A 4	0.9526	250.45
A 3	0.7824	260.35			A 4	0.9625	260.37
A 3	0.7836	270.36			A 4	0.9727	270.38
A 3	0.7881	280.36			A 4	0.986	280.38
A 3	0.791	290.37			A 4	0.9969	290.39
A 3	0.7942	300.38			A 4	1.0086	300.4
B 3	0.6834	0.46	4.918248592854368E-4	0.9966977231001345	B 4	0.6546	0.45	0.0012388184692650398	0.9981093830630642
B 3	0.6781	10.9			B 4	0.6582	10.88
B 3	0.6849	20.81			B 4	0.672	20.79
B 3	0.6899	30.82			B 4	0.6859	30.8
B 3	0.6961	40.83			B 4	0.7009	40.81
B 3	0.7018	50.83			B 4	0.7145	50.82
B 3	0.7075	60.84			B 4	0.7282	60.82
B 3	0.7131	70.85			B 4	0.7425	70.83
B 3	0.718	80.86			B 4	0.7553	80.84
B 3	0.7236	90.86			B 4	0.7688	90.85
B 3	0.7284	100.87			B 4	0.782	100.85
B 3	0.7337	110.88			B 4	0.7951	110.86
B 3	0.7391	120.89			B 4	0.8081	120.87
B 3	0.7441	130.89			B 4	0.8207	130.88
B 3	0.749	140.81			B 4	0.833	140.79
B 3	0.7545	150.82			B 4	0.8458	150.8
B 3	0.7598	160.83			B 4	0.8583	160.81
B 3	0.764	170.83			B 4	0.8705	170.82
B 3	0.7694	180.84			B 4	0.8828	180.82
B 3	0.7744	190.85			B 4	0.8948	190.83
B 3	0.7786	200.86			B 4	0.9066	200.84
B 3	0.7837	210.86			B 4	0.9186	210.85
B 3	0.7882	220.87			B 4	0.93	220.85
B 3	0.7929	230.88			B 4	0.9413	230.86
B 3	0.797	240.89			B 4	0.9525	240.87
B 3	0.801	250.89			B 4	0.9643	250.88
B 3	0.8051	260.81			B 4	0.9742	260.79
B 3	0.8089	270.82			B 4	0.985	270.8
B 3	0.8129	280.83			B 4	0.996	280.81
B 3	0.8161	290.83			B 4	1.0065	290.82
B 3	0.8203	300.84			B 4	1.0176	300.82
C 3	0.6627	0.89	4.5558363151809283E-4	0.9802658025224887	C 4	0.6458	0.91	0.0012377631466940243	0.999152834584321
C 3	0.6549	11.32			C 4	0.6535	11.34
C 3	0.6583	21.24			C 4	0.6748	21.26
C 3	0.6638	31.24			C 4	0.6885	31.26
C 3	0.6711	41.25			C 4	0.6997	41.27
C 3	0.6795	51.26			C 4	0.7126	51.28
C 3	0.6868	61.27			C 4	0.7253	61.29
C 3	0.6942	71.27			C 4	0.7377	71.29
C 3	0.6987	81.28			C 4	0.7506	81.3
C 3	0.7053	91.29			C 4	0.7635	91.31
C 3	0.7089	101.3			C 4	0.7774	101.32
C 3	0.7153	111.3			C 4	0.7889	111.32
C 3	0.7196	121.31			C 4	0.8017	121.33
C 3	0.7245	131.32			C 4	0.8145	131.34
C 3	0.7292	141.24			C 4	0.8273	141.25
C 3	0.7346	151.24			C 4	0.84	151.26
C 3	0.7394	161.25			C 4	0.8525	161.27

↑ Previous 1 2 3 Next ↓

100

after 1 hour of incubation					mM/min
LS_ATA V37A					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A5	V37A_pH 8	4.5990072221825873E-4	0.9981182301028454	0.027594043333095526	0.003015742440775467	40	0.12062969763101868	0.12327300709929301	0.002415887221377865
B5	V37A_pH 8	4.779609250230622E-4	0.9995416598619934	0.028677655501383733	0.0031341700001512275	40	0.1253668000060491
C5	V37A_pH 8	4.720733714568429E-4	0.999184873755018	0.028324402287410577	0.0030955630915202813	40	0.12382252366081126

D5	V37A_pH 9	9.593973512100964E-4	0.9997955358829912	0.05756384107260579	0.006291130171869485	40	0.25164520687477937	0.25406342473788257	0.004164240332139905
E5	V37A_pH 9	9.869489208540639E-4	0.999761240199501	0.05921693525124383	0.00647179620232173	40	0.2588718480928692
F5	V37A_pH 9	9.595041483753721E-4	0.999744921276701	0.057570248902522325	0.006291830481149981	40	0.2516732192459992

A6	V37A_pH 10	0.0011388443165725219	0.9987269614657291	0.06833065899435131	0.00746783158408211	40	0.2987132633632844	0.3028677441602239	0.004924349821720339
B6	V37A_pH 10	0.0011497843381743777	0.9986497272868535	0.06898706029046266	0.007539569430651656	40	0.30158277722606625
C6	V37A_pH 10	0.0011754211690856611	0.9983469230644096	0.07052527014513967	0.007707679797283023	40	0.30830719189132094

G5	blank KPi	2.631317777650959E-5	0.9089696254364421
H5	blank CHES	2.8773224934368343E-5	0.779842057871121

Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared	Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared
A 5	0.6608	0	4.5990072221825873E-4	0.9981182301028454	A 6	0.6472	0.02	0.0011388443165725219	0.9987269614657291
A 5	0.6527	10.4			A 6	0.6499	10.42
A 5	0.6547	20.35			A 6	0.661	20.37
A 5	0.6584	30.4			A 6	0.6736	30.42
A 5	0.6621	40.35			A 6	0.6868	40.37
A 5	0.6669	50.4			A 6	0.6997	50.41
A 5	0.6715	60.35			A 6	0.7128	60.37
A 5	0.6757	70.39			A 6	0.7257	70.41
A 5	0.6798	80.35			A 6	0.7385	80.36
A 5	0.6831	90.39			A 6	0.7507	90.41
A 5	0.6879	100.34			A 6	0.7635	100.36
A 5	0.6906	110.39			A 6	0.7745	110.41
A 5	0.6945	120.34			A 6	0.7856	120.36
A 5	0.6997	130.39			A 6	0.7965	130.4
A 5	0.7055	140.34			A 6	0.8084	140.36
A 5	0.7102	150.38			A 6	0.819	150.4
A 5	0.7163	160.34			A 6	0.8307	160.36
A 5	0.7208	170.38			A 6	0.8419	170.4
A 5	0.7266	180.33			A 6	0.8534	180.35
A 5	0.7304	190.38			A 6	0.8631	190.4
A 5	0.7355	200.33			A 6	0.8745	200.35
A 5	0.741	210.38			A 6	0.8857	210.39
A 5	0.7449	220.33			A 6	0.8972	220.35
A 5	0.7493	230.37			A 6	0.9069	230.39
A 5	0.7536	240.33			A 6	0.9178	240.35
A 5	0.759	250.37			A 6	0.9286	250.39
A 5	0.7633	260.32			A 6	0.9385	260.34
A 5	0.7687	270.37			A 6	0.9501	270.39
A 5	0.7726	280.32			A 6	0.9595	280.34
A 5	0.7775	290.37			A 6	0.971	290.39
A 5	0.7825	300.32			A 6	0.9815	300.34
B 5	0.6706	0.47	4.779609250230622E-4	0.9995416598619934	B 6	0.6364	0.45	0.0011497843381743777	0.9986497272868535
B 5	0.6633	10.86			B 6	0.6459	10.84
B 5	0.6653	20.82			B 6	0.6579	20.8
B 5	0.6709	30.86			B 6	0.6709	30.84
B 5	0.6761	40.81			B 6	0.6844	40.79
B 5	0.681	50.86			B 6	0.6982	50.84
B 5	0.6857	60.81			B 6	0.7113	60.79
B 5	0.6905	70.85			B 6	0.7237	70.84
B 5	0.6951	80.81			B 6	0.7356	80.79
B 5	0.7004	90.85			B 6	0.7478	90.83
B 5	0.7048	100.81			B 6	0.7594	100.79
B 5	0.7089	110.85			B 6	0.7713	110.83
B 5	0.7128	120.8			B 6	0.7835	120.79
B 5	0.7169	130.85			B 6	0.7947	130.83
B 5	0.7215	140.8			B 6	0.8064	140.78
B 5	0.7263	150.85			B 6	0.8179	150.83
B 5	0.7316	160.8			B 6	0.8297	160.78
B 5	0.7369	170.84			B 6	0.8409	170.83
B 5	0.7424	180.8			B 6	0.8524	180.78
B 5	0.7473	190.84			B 6	0.8635	190.82
B 5	0.7527	200.79			B 6	0.8746	200.78
B 5	0.7571	210.84			B 6	0.8853	210.82
B 5	0.7624	220.79			B 6	0.8966	220.77
B 5	0.7671	230.84			B 6	0.9072	230.82
B 5	0.7717	240.79			B 6	0.9178	240.77
B 5	0.7764	250.83			B 6	0.9286	250.82
B 5	0.7808	260.79			B 6	0.9387	260.77
B 5	0.786	270.83			B 6	0.9493	270.81
B 5	0.7904	280.78			B 6	0.9591	280.77
B 5	0.7958	290.83			B 6	0.97	290.81
B 5	0.8001	300.78			B 6	0.9799	300.76
C 5	0.6857	0.89	4.720733714568429E-4	0.999184873755018	C 6	0.6441	0.91	0.0011754211690856611	0.9983469230644096
C 5	0.6779	11.29			C 6	0.6436	11.31
C 5	0.6783	21.24			C 6	0.6581	21.26
C 5	0.6821	31.29			C 6	0.6738	31.3
C 5	0.687	41.24			C 6	0.689	41.26
C 5	0.692	51.28			C 6	0.7027	51.3
C 5	0.6972	61.24			C 6	0.716	61.25
C 5	0.7014	71.28			C 6	0.7284	71.3
C 5	0.7062	81.23			C 6	0.7399	81.25
C 5	0.7108	91.28			C 6	0.7516	91.3
C 5	0.7155	101.23			C 6	0.7637	101.25
C 5	0.7202	111.28			C 6	0.7757	111.29
C 5	0.7243	121.23			C 6	0.7875	121.25
C 5	0.7288	131.27			C 6	0.7993	131.29
C 5	0.7329	141.23			C 6	0.8114	141.24
C 5	0.7371	151.27			C 6	0.8231	151.29
C 5	0.7424	161.22			C 6	0.8355	161.24
C 5	0.7479	171.27			C 6	0.8478	171.29

↑ Previous 1 2 3 Next ↓

100

after 5 hours of incubation					mM/min
LS_ATA V37A					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A7	V37A_pH 8	5.256196679544011E-4	0.9998443321992725	0.03153718007726406	0.003446686347241974	40	0.13786745388967897	0.1436369880356361	0.00569779001817054
B7	V37A_pH 8	5.481738093135239E-4	0.9992083690101946	0.032890428558811434	0.0035945823561542548	40	0.1437832942461702
C7	V37A_pH 8	5.690545733896628E-4	0.9996761511302229	0.03414327440337977	0.0037315053992764773	40	0.1492602159710591

D7	V37A_pH 9	0.0010538955569679117	0.999085358790568	0.0632337334180747	0.006910790537494503	40	0.2764316214997801	0.27719708404068455	0.0011270551551225544
E7	V37A_pH 9	0.0010547980119435777	0.9996530569799741	0.06328788071661466	0.0069167082750398534	40	0.27666833100159416
F7	V37A_pH 9	0.0010617480798038403	0.9987014733710946	0.06370488478823041	0.006962282490516985	40	0.2784912996206794

A8	V37A_pH 10	9.79648902577471E-4	0.9977696615448766	0.05877893415464826	0.0064239272300162025	40	0.2569570892006481	0.2648951036278375	0.007441321543483378
B8	V37A_pH 10	0.0010141843428218907	0.9985168359335725	0.06085106056931344	0.0066503891332583	40	0.266015565330332
C8	V37A_pH 10	0.00103590450234403	0.9983832297829033	0.062154270140641794	0.006792816408813311	40	0.27171265635253244

G7	blank KPi	3.1675956579806344E-6	0.0640161294752702
H7	blank CHES	6.303599864910989E-6	0.37888970969241403

Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared	Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared
A 7	0.6863	0	5.256196679544011E-4	0.9998443321992725	A 8	0.6762	0.02	9.79648902577471E-4	0.9977696615448766
A 7	0.6982	10.36			A 8	0.6874	10.38
A 7	0.7026	20.35			A 8	0.6982	20.37
A 7	0.7077	30.34			A 8	0.7116	30.36
A 7	0.7143	40.33			A 8	0.7241	40.35
A 7	0.7198	50.32			A 8	0.7356	50.34
A 7	0.725	60.31			A 8	0.7459	60.33
A 7	0.7306	70.3			A 8	0.7569	70.32
A 7	0.7357	80.29			A 8	0.7684	80.31
A 7	0.7408	90.28			A 8	0.7784	90.3
A 7	0.7466	100.27			A 8	0.7887	100.29
A 7	0.7514	110.35			A 8	0.7987	110.37
A 7	0.7573	120.34			A 8	0.8095	120.36
A 7	0.7629	130.33			A 8	0.8198	130.35
A 7	0.7676	140.32			A 8	0.8287	140.34
A 7	0.7719	150.31			A 8	0.8376	150.33
A 7	0.7777	160.3			A 8	0.8473	160.32
A 7	0.7827	170.29			A 8	0.8574	170.31
A 7	0.7881	180.28			A 8	0.8663	180.3
A 7	0.793	190.27			A 8	0.8754	190.29
A 7	0.7982	200.35			A 8	0.8851	200.37
A 7	0.8042	210.34			A 8	0.8955	210.36
A 7	0.8092	220.33			A 8	0.9044	220.35
A 7	0.814	230.32			A 8	0.9133	230.34
A 7	0.8198	240.31			A 8	0.9225	240.33
A 7	0.8252	250.3			A 8	0.9313	250.32
A 7	0.8297	260.29			A 8	0.9399	260.31
A 7	0.8353	270.28			A 8	0.9485	270.3
A 7	0.8401	280.27			A 8	0.9571	280.29
A 7	0.8451	290.35			A 8	0.9653	290.37
A 7	0.8503	300.34			A 8	0.975	300.36
B 7	0.7054	0.46	5.481738093135239E-4	0.9992083690101946	B 8	0.6772	0.44	0.0010141843428218907	0.9985168359335725
B 7	0.7118	10.82			B 8	0.6909	10.8
B 7	0.7183	20.81			B 8	0.7038	20.79
B 7	0.724	30.8			B 8	0.7151	30.78
B 7	0.7304	40.79			B 8	0.7277	40.77
B 7	0.7367	50.78			B 8	0.7385	50.76
B 7	0.7423	60.77			B 8	0.7493	60.75
B 7	0.7488	70.76			B 8	0.7602	70.74
B 7	0.7546	80.75			B 8	0.7713	80.73
B 7	0.7603	90.74			B 8	0.7824	90.72
B 7	0.7653	100.73			B 8	0.7926	100.71
B 7	0.7712	110.81			B 8	0.8035	110.79
B 7	0.7764	120.8			B 8	0.814	120.78
B 7	0.7826	130.79			B 8	0.8246	130.77
B 7	0.7874	140.78			B 8	0.8346	140.76
B 7	0.7932	150.77			B 8	0.8448	150.75
B 7	0.799	160.76			B 8	0.8551	160.74
B 7	0.8041	170.75			B 8	0.8647	170.73
B 7	0.8097	180.74			B 8	0.8748	180.72
B 7	0.8145	190.73			B 8	0.8841	190.71
B 7	0.82	200.81			B 8	0.895	200.79
B 7	0.825	210.8			B 8	0.904	210.78
B 7	0.8304	220.79			B 8	0.9133	220.77
B 7	0.8358	230.78			B 8	0.9226	230.76
B 7	0.8407	240.77			B 8	0.9324	240.75
B 7	0.8464	250.76			B 8	0.9414	250.74
B 7	0.8513	260.75			B 8	0.9506	260.73
B 7	0.8564	270.74			B 8	0.9599	270.72
B 7	0.862	280.73			B 8	0.9694	280.71
B 7	0.867	290.81			B 8	0.9781	290.8
B 7	0.872	300.8			B 8	0.987	300.78
C 7	0.6866	0.88	5.690545733896628E-4	0.9996761511302229	C 8	0.6815	0.9	0.00103590450234403	0.9983832297829033
C 7	0.6887	11.24			C 8	0.6969	11.26
C 7	0.6945	21.23			C 8	0.7091	21.25
C 7	0.7017	31.22			C 8	0.7214	31.24
C 7	0.7077	41.21			C 8	0.7331	41.23
C 7	0.7133	51.2			C 8	0.7446	51.22
C 7	0.7189	61.19			C 8	0.7561	61.21
C 7	0.7251	71.18			C 8	0.7676	71.2
C 7	0.7315	81.17			C 8	0.779	81.19
C 7	0.7366	91.16			C 8	0.79	91.18
C 7	0.7423	101.15			C 8	0.8008	101.17
C 7	0.7486	111.24			C 8	0.8143	111.25
C 7	0.754	121.23			C 8	0.8228	121.24
C 7	0.7594	131.21			C 8	0.8329	131.23
C 7	0.7654	141.2			C 8	0.8435	141.22
C 7	0.7714	151.19			C 8	0.8536	151.21
C 7	0.777	161.18			C 8	0.864	161.2
C 7	0.7833	171.17			C 8	0.8741	171.19

↑ Previous 1 2 3 Next ↓

100

after 7 hours of incubation					mM/min
LS_ATA V37A					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A9	V37A_pH 8	5.911242943105972E-4	0.9984924328040213	0.035467457658635834	0.0038762248807252277	40	0.1550489952290091	0.15181203827035628	0.003375439196510291
B9	V37A_pH 8	5.65444663961504E-4	0.9978087312954448	0.03392667983769024	0.003707833862042649	40	0.14831335448170596
C9	V37A_pH 8	5.797812294450988E-4	0.9997235004130065	0.03478687376670593	0.0038018441275088446	40	0.15207376510035378

D9	V37A_pH 9	0.0011056375373247067	0.9991778715377899	0.0663382522394824	0.00725008221196529	40	0.29000328847861157	0.2931707005024668	0.0039009098917662326
E9	V37A_pH 9	0.001113176916364131	0.9991303297603475	0.06679061498184787	0.007299520763043482	40	0.2919808305217393
F9	V37A_pH 9	0.0011343254333081264	0.9991009176201349	0.06805952599848758	0.007438199562676238	40	0.2975279825070495

A10	V37A_pH 10	0.0010277025637039812	0.9984584301497251	0.06166215382223887	0.00673903320461627	40	0.26956132818465084	0.27177555332798004	0.002462264739874457
B10	V37A_pH 10	0.0010344765443958714	0.9981478501950244	0.06206859266375228	0.006783452750136861	40	0.2713381100054745
C10	V37A_pH 10	0.0010462537830889186	0.9966693272763796	0.06277522698533512	0.0068606805448453686	40	0.27442722179381474

G9	blank KPi	2.8790402360419987E-5	0.9390469287700254
H9	blank CHES	5.98412881478762E-6	0.4397586040974787

Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared	Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared
A 9	0.7058	0	5.911242943105972E-4	0.9984924328040213	A 10	0.688	0.02	0.0010277025637039812	0.9984584301497251
A 9	0.705	10.32			A 10	0.6946	10.34
A 9	0.7105	20.26			A 10	0.7075	20.28
A 9	0.7169	30.29			A 10	0.7171	30.31
A 9	0.7241	40.31			A 10	0.7289	40.33
A 9	0.7311	50.25			A 10	0.741	50.27
A 9	0.7384	60.28			A 10	0.7519	60.3
A 9	0.7448	70.3			A 10	0.7639	70.32
A 9	0.7516	80.24			A 10	0.7752	80.26
A 9	0.7576	90.27			A 10	0.7862	90.29
A 9	0.7632	100.29			A 10	0.7969	100.31
A 9	0.7692	110.32			A 10	0.8075	110.34
A 9	0.7759	120.26			A 10	0.8184	120.28
A 9	0.7813	130.28			A 10	0.829	130.3
A 9	0.7877	140.31			A 10	0.8399	140.33
A 9	0.7938	150.25			A 10	0.8502	150.27
A 9	0.7995	160.27			A 10	0.8611	160.29
A 9	0.805	170.3			A 10	0.8712	170.32
A 9	0.811	180.24			A 10	0.8796	180.26
A 9	0.8164	190.26			A 10	0.8896	190.28
A 9	0.821	200.29			A 10	0.8985	200.31
A 9	0.8273	210.32			A 10	0.9077	210.34
A 9	0.8328	220.25			A 10	0.9181	220.27
A 9	0.8388	230.28			A 10	0.9275	230.3
A 9	0.8449	240.31			A 10	0.938	240.33
A 9	0.8494	250.24			A 10	0.9473	250.26
A 9	0.8554	260.27			A 10	0.9577	260.29
A 9	0.8609	270.3			A 10	0.9659	270.32
A 9	0.8655	280.32			A 10	0.9756	280.34
A 9	0.8709	290.26			A 10	0.9841	290.28
A 9	0.8778	300.29			A 10	0.9945	300.31
B 9	0.6963	0.46	5.65444663961504E-4	0.9978087312954448	B 10	0.6735	0.44	0.0010344765443958714	0.9981478501950244
B 9	0.6971	10.79			B 10	0.683	10.77
B 9	0.7038	20.72			B 10	0.6952	20.7
B 9	0.7099	30.75			B 10	0.708	30.73
B 9	0.7172	40.78			B 10	0.7214	40.76
B 9	0.7239	50.71			B 10	0.7329	50.69
B 9	0.7308	60.74			B 10	0.7445	60.72
B 9	0.7371	70.77			B 10	0.7557	70.75
B 9	0.7436	80.7			B 10	0.767	80.68
B 9	0.7492	90.73			B 10	0.7775	90.71
B 9	0.7551	100.76			B 10	0.7885	100.74
B 9	0.761	110.78			B 10	0.7995	110.76
B 9	0.7669	120.72			B 10	0.81	120.7
B 9	0.7729	130.75			B 10	0.8212	130.73
B 9	0.7783	140.77			B 10	0.831	140.75
B 9	0.7842	150.71			B 10	0.8416	150.69
B 9	0.7897	160.74			B 10	0.8521	160.72
B 9	0.795	170.76			B 10	0.8616	170.75
B 9	0.8002	180.7			B 10	0.8712	180.68
B 9	0.8054	190.73			B 10	0.8816	190.71
B 9	0.8109	200.75			B 10	0.8917	200.73
B 9	0.8164	210.78			B 10	0.9013	210.76
B 9	0.8216	220.72			B 10	0.9107	220.7
B 9	0.8267	230.74			B 10	0.9196	230.72
B 9	0.8322	240.77			B 10	0.9301	240.75
B 9	0.8371	250.71			B 10	0.9389	250.69
B 9	0.8421	260.73			B 10	0.9503	260.71
B 9	0.8474	270.76			B 10	0.9585	270.74
B 9	0.8529	280.79			B 10	0.9676	280.77
B 9	0.8573	290.72			B 10	0.9767	290.7
B 9	0.863	300.75			B 10	0.986	300.73
C 9	0.6975	0.89	5.797812294450988E-4	0.9997235004130065	C 10	0.671	0.91	0.0010462537830889186	0.9966693272763796
C 9	0.7051	11.21			C 10	0.6754	11.23
C 9	0.712	21.15			C 10	0.6868	21.17
C 9	0.7166	31.17			C 10	0.7002	31.19
C 9	0.7225	41.2			C 10	0.7118	41.22
C 9	0.7288	51.14			C 10	0.7248	51.16
C 9	0.7361	61.16			C 10	0.737	61.18
C 9	0.7417	71.19			C 10	0.749	71.21
C 9	0.7479	81.13			C 10	0.7623	81.15
C 9	0.7524	91.15			C 10	0.7728	91.17
C 9	0.7581	101.18			C 10	0.784	101.2
C 9	0.7638	111.21			C 10	0.7959	111.23
C 9	0.77	121.14			C 10	0.8067	121.16
C 9	0.7765	131.17			C 10	0.8175	131.19
C 9	0.7815	141.2			C 10	0.8276	141.22
C 9	0.7885	151.13			C 10	0.8383	151.15
C 9	0.7944	161.16			C 10	0.8491	161.18
C 9	0.7996	171.19			C 10	0.8591	171.21

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100

after 24 hours of incubation					mM/min
LS_ATA V37A					mM/min
R-PEA				*60	(ΔΑbs/min) / (9,15 Αbs/mM)	40	*αραίωση πηγαδιού	μmol/(ml * min)		(Bradford)	μmol/ (mg * min)
Well	Sample	ΔΑbs/s	R sqrd	ΔΑbs/min	mmol/ (L * min)	αραιωση πηγαδιού	mmol/ (L * min)	U /ml	STDEV	Συγκέντρωση (mg/ml)	U/mg	STDEV
A1	V37A_pH 8	4.5415663588523503E-4	0.9994947238718537	0.027249398153114102	0.002978076300886787	40	0.11912305203547148	0.12031520569524523	0.0026923029919193237
B1	V37A_pH 8	4.51494567409666E-4	0.9993240021425754	0.02708967404457996	0.002960620114161744	40	0.11842480456646975
C1	V37A_pH 8	4.7045396184446645E-4	0.9973119553163832	0.02822723771066799	0.003084944012094862	40	0.12339776048379449

D1	V37A_pH 9	9.97226856054291E-4	0.9996978118756655	0.05983361136325746	0.006539192498716662	40	0.26156769994866647	0.2613694184630786	6.260059100852637E-4
E1	V37A_pH 9	9.937978318014433E-4	0.9987016616474389	0.0596278699080866	0.006516707093779956	40	0.2606682837511982
F1	V37A_pH 9	9.983880358157276E-4	0.999512575689697	0.05990328214894365	0.006546806792234279	40	0.26187227168937116

A5	V37A_pH 10	0.0010597775305895437	0.998719758193114	0.06358665183537263	0.006949360856324877	40	0.27797443425299506	0.27863907073433136	0.0021772799664530216
B5	V37A_pH 10	0.0010715839822024473	0.9988027511777052	0.06429503893214683	0.007026780211163588	40	0.28107120844654354
C5	V37A_pH 10	0.0010555728587319242	0.9986002054949648	0.06333437152391545	0.006921789237586388	40	0.27687156950345554

G1	blank KPi	-1.0856104046004154E-5	0.0778969855019793	5.98412881478762E-6
H1	blank CHES	-2.8977715861646116E-6	0.647446411323261



Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared	Well	Raw absorbance	Measurement time(s)	SLOPE sample	R squared
A 1	1.0583	0	4.5415663588523503E-4	0.9994947238718537	A 5	0.9182	0	0.0010597775305895437	0.998719758193114
A 1	1.0372	10.47			A 5	0.9301	10
A 1	1.0333	20.44			A 5	0.9408	20
A 1	1.0342	30.4			A 5	0.9529	30
A 1	1.037	40.47			A 5	0.9653	40
A 1	1.042	50.44			A 5	0.9771	50
A 1	1.0463	60.4			A 5	0.9889	60
A 1	1.0507	70.47			A 5	1.0006	70
A 1	1.0553	80.44			A 5	1.0131	80
A 1	1.0608	90.4			A 5	1.0233	90
A 1	1.0661	100.47			A 5	1.0344	100
A 1	1.0711	110.44			A 5	1.0458	110
A 1	1.0752	120.4			A 5	1.0557	120
A 1	1.0783	130.47			A 5	1.0671	130
A 1	1.0822	140.44			A 5	1.0774	140
A 1	1.0892	150.4			A 5	1.0875	150
A 1	1.0941	160.47			A 5	1.0981	160
A 1	1.0973	170.44			A 5	1.1089	170
A 1	1.1013	180.4			A 5	1.12	180
A 1	1.1063	190.47			A 5	1.1304	190
A 1	1.1111	200.44			A 5	1.1408	200
A 1	1.1157	210.4			A 5	1.1501	210
A 1	1.1194	220.47			A 5	1.1602	220
A 1	1.1232	230.44			A 5	1.1702	230
A 1	1.1286	240.4			A 5	1.1799	240
A 1	1.1331	250.47			A 5	1.19	250
A 1	1.1371	260.44			A 5	1.1998	260
A 1	1.1415	270.4			A 5	1.2098	270
A 1	1.1478	280.47			A 5	1.2195	280
A 1	1.1512	290.44			A 5	1.2277	290
A 1	1.1555	300.4			A 5	1.237	300
B 1	1.0553	0.47	4.51494567409666E-4	0.9993240021425754	B 5	0.9336	0.43	0.0010715839822024473	0.9988027511777052
B 1	1.0481	10.93			B 5	0.9466	10.43
B 1	1.047	20.9			B 5	0.9588	20.43
B 1	1.0499	30.86			B 5	0.9713	30.43
B 1	1.0547	40.93			B 5	0.9842	40.43
B 1	1.0586	50.9			B 5	0.9957	50.43
B 1	1.0646	60.86			B 5	1.0076	60.43
B 1	1.0691	70.93			B 5	1.019	70.43
B 1	1.0739	80.9			B 5	1.03	80.43
B 1	1.078	90.86			B 5	1.0413	90.43
B 1	1.0839	100.93			B 5	1.053	100.43
B 1	1.0893	110.9			B 5	1.0634	110.43
B 1	1.0938	120.86			B 5	1.075	120.43
B 1	1.0971	130.93			B 5	1.0859	130.43
B 1	1.1012	140.9			B 5	1.0967	140.43
B 1	1.1072	150.86			B 5	1.1074	150.43
B 1	1.1108	160.93			B 5	1.1177	160.43
B 1	1.115	170.9			B 5	1.1289	170.43
B 1	1.1191	180.86			B 5	1.1393	180.43
B 1	1.1245	190.93			B 5	1.1494	190.43
B 1	1.1288	200.9			B 5	1.16	200.43
B 1	1.1339	210.86			B 5	1.1699	210.43
B 1	1.1369	220.93			B 5	1.1805	220.43
B 1	1.142	230.9			B 5	1.1907	230.43
B 1	1.1471	240.86			B 5	1.2005	240.43
B 1	1.1509	250.93			B 5	1.2103	250.43
B 1	1.1549	260.9			B 5	1.2207	260.43
B 1	1.1587	270.86			B 5	1.2298	270.43
B 1	1.1647	280.93			B 5	1.2397	280.43
B 1	1.1681	290.9			B 5	1.2493	290.43
B 1	1.172	300.86			B 5	1.2581	300.43
C 1	1.0454	0.89	4.7045396184446645E-4	0.9973119553163832	C 5	0.9275	0.85	0.0010555728587319242	0.9986002054949648
C 1	1.0263	11.36			C 5	0.9445	10.85
C 1	1.0291	21.32			C 5	0.9585	20.85
C 1	1.0363	31.29			C 5	0.9694	30.85
C 1	1.042	41.36			C 5	0.9816	40.85
C 1	1.0485	51.32			C 5	0.9937	50.85
C 1	1.0522	61.29			C 5	1.0056	60.85
C 1	1.0571	71.36			C 5	1.0171	70.85
C 1	1.0648	81.32			C 5	1.028	80.85
C 1	1.0695	91.29			C 5	1.0399	90.85
C 1	1.0761	101.36			C 5	1.0509	100.85
C 1	1.0816	111.32			C 5	1.0619	110.85
C 1	1.0842	121.29			C 5	1.0722	120.85
C 1	1.0892	131.36			C 5	1.0828	130.85
C 1	1.0933	141.32			C 5	1.0936	140.85
C 1	1.0994	151.29			C 5	1.1043	150.85

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