Selected Cell
Cell:
Value:
target gene dsMesh_SYBR
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| Data | Standard curve | |||||||||||||||||||||||||||
| Involved | Sample name | Cq value | Average Cq value | Replicates Δ Cq | Relative quantity | log10(Relative quantity) | Slope | Slope difference | Calculated copy number | CV copy no. | Average copy no. | dilution corrected copy number | CV dilutions | Reference copy number | Normalized target copy number | Final result | CV Normalized target copy number | Warning | Involved | Cq value | Relative quantity | Cq | Replicates Δ Cq | Relative quantity | log10(Relative quantity) | Parcial slope | ||
| no | HT115::dsGFP | / | / | 10000 | 4 | / | / | / | / | / | / | / | / | / | / | / | yes | 9.79 | 10000 | 9.79 | 0.15 | 10000 | 4 | -3.38999999999999 | ||||
| no | HT115::dsGFP | 10000 | 4 | / | / | yes | 9.94 | 10000 | 9.94 | 10000 | 4 | |||||||||||||||||
| no | HT115::dsGFP | / | / | 1000 | 3 | / | / | / | / | / | / | yes | 13.22 | 1000 | 13.22 | 0.07 | 1000 | 3 | -3.62500000000001 | |||||||||
| no | HT115::dsGFP | 1000 | 3 | / | / | yes | 13.29 | 1000 | 13.29 | 1000 | 3 | |||||||||||||||||
| no | HT115::dsGFP | / | / | 100 | 2 | / | / | / | / | / | / | yes | 16.8 | 100 | 16.8 | 0.16 | 100 | 2 | -3.435 | |||||||||
| no | HT115::dsGFP | 100 | 2 | / | / | yes | 16.96 | 100 | 16.96 | 100 | 2 | |||||||||||||||||
| yes | HT115::dsMesh | 8.95 | 8.95 | / | 10000 | 4 | -3.28857142857143 | 0.23042857142858 | 17545.34482984864 | / | 17545.34482984864 | 1754.534482984864 | 15.53425188560625 | 1226.932448463975 | 1.43001718243038 | / | 19.22664056079566 | yes | 20.29 | 10 | 20.29 | 0.05 | 10 | 1 | -3.615 | |||
| no | HT115::dsMesh | 9.97 | 10000 | 4 | / | / | yes | 20.34 | 10 | 20.34 | 10 | 1 | ||||||||||||||||
| yes | HT115::dsMesh | 12.08 | 12.19 | 0.22 | 1000 | 3 | 2264.157920468495 | 10.15913482353318 | 2112.410915198686 | 2112.410915198686 | 1442.714513269711 | 1.46419190752521 | yes | 23.94 | 1 | 23.94 | 0.02 | 1 | 0 | / | ||||||||
| yes | HT115::dsMesh | 12.3 | 1000 | 3 | 1960.663909928877 | 10.15913482353318 | yes | 23.92 | 1 | 23.92 | 1 | 0 | ||||||||||||||||
| yes | HT115::dsMesh | 15.38 | 15.515 | 0.27 | 100 | 2 | 261.4287317149412 | 12.45717295790064 | 240.2648764053663 | 2402.648764053663 | 1208.407789105647 | 1.9882764623951 | ||||||||||||||||
| yes | HT115::dsMesh | 15.65 | 100 | 2 | 219.1010210957914 | 12.45717295790064 | ||||||||||||||||||||||
| yes | dsMesh in-vitro | 14.81 | 14.77 | 0.08 | 10000 | 4 | -3.72250000000002 | 0.20350000000001 | 379.572165521654 | 3.69976076419053 | 389.7690137381702 | 38.97690137381702 | 23.86236903657089 | / | / | / | / | Slope: | -3.519 | |||||||||
| yes | dsMesh in-vitro | 14.73 | 10000 | 4 | 399.9658619546865 | 3.69976076419053 | Inverse slope: | -0.28410709053066 | ||||||||||||||||||||
| yes | dsMesh in-vitro | 17.92 | 17.945 | 0.05 | 1000 | 3 | 49.62737521636136 | 2.31267204075673 | 48.82887367504933 | 48.82887367504933 | / | / | Correlation cofficient (rsq): | 0.99977285157738 | ||||||||||||||
| yes | dsMesh in-vitro | 17.97 | 1000 | 3 | 48.0303721337373 | 2.31267204075673 | Intercept: | 6.78692036835103 | ||||||||||||||||||||
| yes | dsMesh in-vitro | 22.04 | 22.215 | 0.35 | 100 | 2 | 3.35119803773114 | 16.11978683416412 | 3.00829968778844 | 30.08299687788436 | / | / | ||||||||||||||||
| yes | dsMesh in-vitro | 22.39 | 100 | 2 | 2.66540133784574 | 16.11978683416412 | ||||||||||||||||||||||
| Experiment | ||||||||||||||||||||||||||||
| Name: | Ecoli_dsRNA_production | |||||||||||||||||||||||||||
| Gene | ||||||||||||||||||||||||||||
| Name: | dsMesh_SYBR | |||||||||||||||||||||||||||
| Type: | target_gene | |||||||||||||||||||||||||||
| Conditions | ||||||||||||||||||||||||||||
| Cq at Limit of quantification (CqLOQ): | 28 | |||||||||||||||||||||||||||
| Slope between: | between(-3.8, -3) | |||||||||||||||||||||||||||
| Slope difference: | 0.5 | |||||||||||||||||||||||||||
| Final result calculation: | two_dilution |
