Selected Cell
Cell:
Value:
Metadata_
Layout_sample wells
Cell count
Amino acids
Translation amino acids
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| Metadata | Notes | ||||||||
| Title experiment | SSZ inhibition on HepG2 cells | The name of the experiment | |||||||
| Experimenter | Jurgen Haanstra | The person that measured the data | |||||||
| Project | The project that the asset belongs to | ||||||||
| Start date | 2018-06-11T0:0:0+0000 | start date; should correspond with an entry in your lab journal | |||||||
| End date | 2018-06-15T0:0:0+0000 | end date; should correspond with an entry in your lab journal | |||||||
| Organism information | |||||||||
| Organism | homo sapiens | Name of the organism | |||||||
| Cell type | liver | If organism is multicellular, specify specific cell type | |||||||
| Strain | name of the strain | ||||||||
| cell line | HepG2 | the specific cell line | |||||||
| Reference | Refer to published papers that describe the construction of the cell line | ||||||||
| Passage number | |||||||||
| Growth conditions | |||||||||
| Growth medium | Standard DMEM (10%FCS, 6 mM Glc, 1.8 mM Gln) | The medium that was used | |||||||
| Carbon source | Glc, Gln, amino acids | Fill in if different from standard medium composition | |||||||
| Date growth medium was made | 2018-06-11T0:0:0+0000 | The data on which the final medium was made | |||||||
| Temperature | 37C | Assay temperature | |||||||
| Conditions | 5% CO2 | Describes important aspects of the grwoth conditions (e.g. shaker, incubator) | |||||||
| Culture vessel | TPP 6 wellsplate | Where did you grow the cells in | |||||||
| pre-culture conditions | p20 | How where the cells pre-grown? | |||||||
| ASSAY | |||||||||
| Assay Title | Cell count | amino acid measurements | The title of an exisitng assay | ||||||
| Assay_type | The assay_type describes the type of experiment you are performing | ||||||||
| Technology_type | counting chamber | Describes the type of instruments and/or equipment used for the experiment | |||||||
| Description | A brief, human readable description. | ||||||||
| Experimentalist | The names of the people who carried out the experiments | ||||||||
| Date | The start date for the experiment if different from the upload date | ||||||||
| SOP | Specifies which SOPs and protocols used to carry out the experiment | ||||||||
| Experimental_conditions | |||||||||
| Item | The name of the experimental condition you are fixing in your experiment (e.g. temperature, concentration, pH etc). If there is more than 1, please list them in columns across the spreadsheet | ||||||||
| Compound (if concentration) | The compound name is only required if the item is concentration. | ||||||||
| Unit | The SI units of the experimental conditions measurements. | ||||||||
| Start_value (optional) | This field is used for recording changes throughout the experiment to measure different conditions (e.g. pH or dilutions) | ||||||||
| End_value (optional) | This field is used for recording changes throughout the experiment to measure different conditions (e.g. pH or dilutions) | ||||||||
| Comments | Additional information that would be useful for people reading this data file | ||||||||
| Culture growth | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | SSZ (glutamate transport inhibition) | Factors Studied are the factors that you deliberately change during the course of your experiment to see the effects. For example, if you measure the concentration of compounds at pH 5, 6 & 7, the factor studied is the pH (i.e. it is about the treatment you apply rather than the measurements you make). | |||||||
| Compound (if concentration) | The compound name is only required if the Factors Studied item is concentration. | ||||||||
| Unit | The SI units of the factors studied measurements | ||||||||
| Start_value (optional) | This field is used for recording changes throughout the experiment for one particular factor studied (e.g. pH in crease from 4 - 7 or dilution rate doubling) | ||||||||
| End_value (optional) | This field is used for recording changes throughout the experiment for one particular factor studied (e.g. pH in crease from 4 - 7 or dilution rate doubling) | ||||||||
| SD (optional) | The standard deviation for the difference in factors studied measurements (optional) | ||||||||
| Comments | Additional information that would be useful for people reading this data file | ||||||||
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| Time (h) | 0 uM SSZ | 100 uM SSZ | 200 uM SSZ | This table decodes which of the 16 wells was harvested at which time point | |||||
| 0 | 13 | 13 | 13 | The solvent was addded to wells 13 and 16 just prior to harvesting | |||||
| 0 | 16 | 16 | 16 | ||||||
| 24.75 | 1 | 2 | 3 | ||||||
| 45.25 | 4 | 5 | 6 | ||||||
| 45.25 | 14 | 15 | |||||||
| 68.75 | 7 | 8 | 9 | ||||||
| 68.75 | 10 | 11 | 12 | ||||||
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| Date | Time | t (h) | 0 uM | 100 uM | 200 uM | Each well is counted twice; if there are 4 counts, there were separate wells counted | |||
| 2018-06-12T0:0:0+0000 | 1899-12-31T12:15:0+0000 | 0 | 300800 | 300800 | 300800 | ||||
| 0 | 291200 | 291200 | 291200 | ||||||
| 0 | 409600 | 409600 | 409600 | ||||||
| 0 | 361600 | 361600 | 361600 | ||||||
| 2018-06-13T0:0:0+0000 | 1899-12-31T13:0:0+0000 | 24.75 | 857600 | 640000 | 556800 | ||||
| 24.75 | 704000 | 704000 | 627200 | ||||||
| 2018-06-14T0:0:0+0000 | 1899-12-31T9:25:0+0000 | 45.25 | 1638400 | 1177600 | 1152000 | ||||
| 45.25 | 1459200 | 1625600 | 1036800 | ||||||
| 45.25 | 1190400 | 1216000 | |||||||
| 45.25 | 1548800 | 1241600 | |||||||
| 2018-06-15T0:0:0+0000 | 1899-12-31T9:0:0+0000 | 68.75 | 2822400 | 2803200 | 2092800 | ||||
| 68.75 | 3148800 | 2976000 | 2707200 | ||||||
| 68.75 | 2438400 | 2841600 | 2515200 | ||||||
| 68.75 | 2342400 | 2764800 | 2707200 | ||||||
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| concentrations are in micromolar | |||||||||||||||||||
| buis | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | |||
| id | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | This is the ID that corresponds to the well number | ||
| Dutch name | |||||||||||||||||||
| Taurine | 10.5661 | 9.8767 | 10.7739 | 7.5879 | 6.5513 | 7.0642 | 5.9684 | 4.668 | 4.9869 | 5.4598 | 5.0293 | 4.9833 | 13.0768 | 7.3076 | 8.0657 | 12.1706 | |||
| Asparaginezuur | 24.8539 | 24.272 | 24.5266 | 28.1534 | 25.4107 | 23.6609 | 27.108 | 20.9213 | 19.7733 | 26.0587 | 21.8265 | 20.3047 | 17.3048 | 33.7134 | 35.4213 | 17.2032 | |||
| Threonine | 689.8435 | 628.8808 | 660.9514 | 632.3158 | 581.0364 | 592.5018 | 534.685 | 505.4579 | 514.8731 | 521.4539 | 519.7681 | 502.7544 | 706.0124 | 605.9745 | 649.2072 | 708.683 | |||
| Serine | 321.5285 | 299.5064 | 314.786 | 309.9675 | 300.7722 | 295.4638 | 323.8843 | 319.7679 | 303.0324 | 304.8349 | 331.6981 | 307.9502 | 362.3693 | 284.0626 | 271.7288 | 364.1488 | |||
| Asparagine | 0.9299 | 2.9909 | 10.869 | 2.592 | 5.2074 | 28.1598 | 1.1881 | 1.9372 | 27.335 | 1.9236 | 2.3444 | 25.3923 | 0.3637 | 2.8961 | 33.3391 | 0.9411 | |||
| Glutaminezuur | 207.0301 | 146.2281 | 139.4949 | 304.7498 | 189.5698 | 161.0543 | 421.7292 | 248.1458 | 208.6594 | 397.7472 | 269.5402 | 225.7019 | 95.8138 | 243.6113 | 234.7282 | 96.9471 | |||
| Glutamine | 1135.4238 | 1037.5467 | 1129.3194 | 620.8379 | 592.6756 | 680.3757 | 169.1914 | 203.7942 | 232.9939 | 180.509 | 200.7761 | 197.4904 | 1572.5509 | 530.6938 | 580.4466 | 1569.4872 | |||
| Proline | 68.6675 | 69.5802 | 62.3533 | 156.4903 | 117.4368 | 100.1948 | 255.3652 | 196.2202 | 173.1107 | 238.9153 | 216.3732 | 202.7583 | 30.485 | 138.3012 | 136.5292 | 27.5992 | |||
| Glycine | 442.4109 | 405.6228 | 412.9112 | 482.3051 | 452.1164 | 440.2008 | 519.3131 | 492.8561 | 498.3841 | 499.3434 | 513.4998 | 514.5921 | 396.3056 | 468.4429 | 526.1684 | 397.535 | |||
| Alanine | 456.1559 | 500.219 | 433.242 | 771.8988 | 803.0678 | 677.3063 | 695.0943 | 756.1764 | 715.9989 | 715.906 | 765.1612 | 660.5132 | 122.3666 | 783.7909 | 807.8325 | 123.4821 | |||
| Citrulline | 9.6026 | 7.8971 | 8.4848 | 7.0275 | 8.0194 | 8.1856 | 7.2455 | 7.2848 | 7.025 | 7.6896 | 6.8595 | 6.8399 | 9.5842 | 7.5851 | 8.1132 | 9.8774 | |||
| a-a-boterzuur | 2.7901 | 2.0986 | 1.4386 | 2.6793 | 3.5238 | 1.9693 | 3.1789 | 3.5304 | 3.9756 | 1.7501 | 3.4577 | 1.9394 | 4.117 | 2.7525 | 3.3444 | 3.8668 | |||
| Valine | 653.4813 | 593.9797 | 624.0096 | 557.2008 | 506.9933 | 519.7724 | 416.5294 | 391.22 | 399.3738 | 415.2042 | 403.7041 | 380.6072 | 701.2014 | 525.499 | 572.5588 | 703.3338 | |||
| Cystine | 118.6082 | 134.2737 | 144.4021 | 68.4977 | 118.5329 | 128.1248 | 24.8014 | 100.5592 | 111.4617 | 25.5665 | 101.676 | 110.393 | 163.9535 | 121.2197 | 139.3905 | 161.0297 | |||
| Methionine | 136.1036 | 124.3658 | 132.1063 | 109.6361 | 98.1055 | 105.0043 | 68.1185 | 61.8047 | 67.2487 | 71.4668 | 62.5924 | 61.1186 | 145.7535 | 102.5542 | 113.2912 | 144.4309 | |||
| iso-leucine | 591.3147 | 544.2549 | 572.9001 | 492.863 | 455.4429 | 469.8349 | 366.562 | 350.4469 | 355.1168 | 366.2279 | 358.3469 | 338.3233 | 670.9737 | 466.7645 | 512.5127 | 673.4458 | |||
| leucine | 591.9677 | 538.4969 | 570.1357 | 466.4734 | 425.5607 | 449.6765 | 308.3128 | 290.0794 | 302.3444 | 314.3677 | 294.4956 | 279.776 | 676.5958 | 437.5684 | 487.9052 | 675.9601 | |||
| Tyrosine | 340.6388 | 316.6312 | 332.9933 | 348.2276 | 330.1659 | 326.7901 | 353.7964 | 341.3342 | 335.1629 | 339.9271 | 351.4864 | 340.9592 | 335.7473 | 343.4357 | 356.3341 | 332.642 | |||
| Phenylalanine | 316.7381 | 284.4865 | 301.9529 | 253.0724 | 209.6393 | 232.0542 | 116.3967 | 98.7507 | 120.5417 | 126.5048 | 100.4955 | 100.7558 | 344.2861 | 223.216 | 250.4789 | 346.2656 | |||
| Ornithine | 44.1702 | 41.0492 | 43.0662 | 64.371 | 56.7857 | 54.2993 | 76.3464 | 68.7095 | 66.2524 | 73.0759 | 70.4439 | 68.2943 | 28.0935 | 61.681 | 69.4453 | 28.8846 | |||
| Lysine | 642.4068 | 589.7644 | 622.9413 | 598.9752 | 526.7784 | 545.4644 | 439.7422 | 414.7418 | 426.7093 | 437.7018 | 424.6582 | 405.5854 | 672.9616 | 548.1092 | 592.403 | 672.9363 | |||
| Histidine | 162.1565 | 147.4553 | 155.45 | 146.5627 | 128.5876 | 135.6923 | 104.9582 | 98.2248 | 102.8275 | 103.7326 | 98.573 | 97.2189 | 170.9222 | 131.3856 | 143.6606 | 170.6285 | |||
| Tryptofaan | 75.16252611935778 | 67.41421967506692 | 70.95570436094296 | 66.19543860513193 | 60.46704199410811 | 65.88355264280126 | 44.62009019575321 | 41.63371451289891 | 45.35846001607798 | 46.59074623533399 | 41.02731036209703 | 40.26499324539869 | 77.4516657231186 | 60.00479918723599 | 64.04542517774132 | 77.56356304228288 | |||
| Arginine | 287.0859 | 257.7604 | 277.7088 | 226.5043 | 196.8663 | 212.8341 | 109.7792 | 105.1448 | 119.5092 | 116.5719 | 106.0135 | 101.1261 | 325.2054 | 196.2864 | 217.6392 | 326.3631 | |||
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| Dutch | English | ||||||||
| Taurine | Taurine | ||||||||
| Asparaginezuur | Aspartic acid | ||||||||
| Threonine | Threonine | ||||||||
| Serine | Serine | ||||||||
| Asparagine | Asparagine | ||||||||
| Glutaminezuur | Glutamate | ||||||||
| Glutamine | Glutamine | ||||||||
| Proline | Proline | ||||||||
| Glycine | Glycine | ||||||||
| Alanine | Alanine | ||||||||
| Citrulline | Citrulline | ||||||||
| a-a-boterzuur | Butyric acid | ||||||||
| Valine | Valine | ||||||||
| Cystine | Cystine | ||||||||
| Methionine | Methionine | ||||||||
| iso-leucine | iso-leucine | ||||||||
| leucine | leucine | ||||||||
| Tyrosine | Tyrosine | ||||||||
| Phenylalanine | Phenylalanine | ||||||||
| Ornithine | Ornithine | ||||||||
| Lysine | Lysine | ||||||||
| Histidine | Histidine | ||||||||
| Tryptofaan | Tryptophan | ||||||||
| Arginine | Arginine | ||||||||
