HepG2 | cell counts and metabolite levels over time at 0, 6 or 22 mM glucose

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Value:

Metadata_ Cell count HPLC_rawdata_161109_newcalibrat HPLC_converted HPLC_glucose_pyruvate Layout_Lac_enzym_0mMGlc raw_Lac_0mM_ph1 raw_Lac_0mM_ph2 raw_lac_0mM_ph3 analysis_lac_0mM Layout_Lac_enzym_6mM raw_Lac_6mM_ph1 raw_Lac6mM_ph2 raw_lac_6mM_ph3 analysis_lac_6 mM Layout_Lac_enzym_22mMglc raw_lac_22mM_ph1 raw_lac_22mM_ph2 raw_lac_22mM_ph3 analysis_lac_22mM Aminoacids_analysis1 Aminoacids_analysis2 Translation amino acids

Metadata					Notes

Title experiment	Different glucose levels on IMOMESIC Hepg2 cells				The name of the experiment
Experimenter	Jurgen Haanstra				The person that measured the data
Project	IMOMESIC				The project that the asset belongs to
Start date	160802				start date; should correspond with an entry in your lab journal
End date	160808				end date; should correspond with an entry in your lab journal


Organism information
Organism	homo sapiens				Name of the organism
Cell type	liver cells				If organism is multicellular, specify specific cell type
Strain					name of the strain
cell line	hepg2				the specific cell line
Reference	ATCC				Refer to published papers that describe the construction of the cell line
Passage number	p14


Growth conditions
Growth medium	0 mM/6 mM/22 mM glucose				The medium that was used
Carbon source	glucose				Fill in if different from standard medium composition
Date growth medium was made	160726 (tested by HPLC)				The data on which the final medium was made
Temperature	37°C				Assay temperature
Conditions	5% CO2, humidified incubator				Describes important aspects of the grwoth conditions (e.g. shaker, incubator)
Culture vessel	TPP 6 wellsplates				Where did you grow the cells in
pre-culture conditions	full medium 6 mM glucose, 10 cm plates				How where the cells pre-grown?


ASSAY
Assay Title	Cell count	glucose, pyruvate	lactate	amino acids	The title of an exisitng assay
Assay_type					The assay_type describes the type of experiment you are performing
Technology_type	Counting chamber	HPLC	enzyme assay		Describes the type of instruments and/or equipment used for the experiment
Description				unit is micromolar	A brief, human readable description.
Experimentalist	Jurgen Haanstra	Jurgen Haanstra	Jurgen Haanstra	Albert Gerding	The names of the people who carried out the experiments
Date					The start date for the experiment if different from the upload date
SOP					Specifies which SOPs and protocols used to carry out the experiment

Experimental_conditions
Item					The name of the experimental condition you are fixing in your experiment (e.g. temperature, concentration, pH etc). If there is more than 1, please list them in columns across the spreadsheet
Compound (if concentration)					The compound name is only required if the item is concentration.
Unit					The SI units of the experimental conditions measurements.
Start_value (optional)					This field is used for recording changes throughout the experiment to measure different conditions (e.g. pH or dilutions)
End_value (optional)					This field is used for recording changes throughout the experiment to measure different conditions (e.g. pH or dilutions)
Comments					Additional information that would be useful for people reading this data file

Culture growth

FACTORS_STUDIED
Item					Factors Studied are the factors that you deliberately change during the course of your experiment to see the effects. For example, if you measure the concentration of compounds at pH 5, 6 & 7, the factor studied is the pH (i.e. it is about the treatment you apply rather than the measurements you make).
Compound (if concentration)					The compound name is only required if the Factors Studied item is concentration.
Unit					The SI units of the factors studied measurements
Start_value (optional)					This field is used for recording changes throughout the experiment for one particular factor studied (e.g. pH in crease from 4 - 7 or dilution rate doubling)
End_value (optional)					This field is used for recording changes throughout the experiment for one particular factor studied (e.g. pH in crease from 4 - 7 or dilution rate doubling)
SD (optional)					The standard deviation for the difference in factors studied measurements (optional)
Comments					Additional information that would be useful for people reading this data file

			0 mM	6 mM	22 mM	Each well is counted in duplicate	average		0 mM	6 mM	22 mM	0 mM	6 mM	22 mM
Date	Time	t	cells	cells	cells	If more data entries are available this denotes multiple wells						cells	cells	cells
160802	1899-12-31T10:0:0+0000	0	499200	499200	499200			0	511466.6666666667	511466.6666666667	511466.6666666667	38846.29540466718	38846.29540466718	38846.29540466718
		0	441600	441600	441600			23.75	947200	1318400	1004800	108611.6015902537	18101.933598375617	171968.36918456835
		0	524800	524800	524800			48	2467200	2659200	2860800	67882.25099390856	67882.25099390856	81458.70119269028
		0	544000	544000	544000			54.5	2150400	2841600	2995200	144815.46878700494	289630.9375740099	217223.2031805074
		0	512000	512000	512000			71.5	2227200	3660800	3328000	615997.9220744174	108611.6015902537	434446.4063610148
		0	547200	547200	547200			144	883200	1324800	198400	54305.80079512685	99560.6347910659	27152.900397563426
160803	1899-12-31T9:40:0+0000	23.75	1024000	1331200	1126400
		23.75	870400	1305600	883200
160804	1899-12-31T9:50:0+0000	48	2515200	2611200	2803200
		48	2419200	2707200	2918400
	1899-12-31T16:25:0+0000	54.5	2252800	3046400	2841600
		54.5	2048000	2636800	3148800
16805	1899-12-31T9:20:0+0000	71.5	2816000	3737600	3635200
		71.5	1587200	3584000	3020800
		71.5	2278400
160808	1899-12-31T9:55:0+0000	144	844800	1254400	217600
		144	921600	1395200	179200

with Hakan's python script
corrected for the dilution factor (1.205)

new calibration
	sample1	sample2	sample3	sample4	sample5	sample6	sample7	sample8	sample9	sample10	sample11	sample12	sample13	sample14	sample15	sample16	sample17	sample18	sample19	sample20	sample21	sample22	sample23	sample24	sample25	sample26	sample27	sample28	sample29	sample30	sample31	sample32	sample33	sample34	sample35	sample36	sample37	sample38	sample39	sample40	sample41	sample42	sample43	sample44	sample45	sample46	sample47	sample48	sample49	sample50	sample51	sample52	sample53	sample54	sample55	sample56	sample57	sample58	sample59	sample60	sample61	sample62	sample63	sample64	sample65	sample66	sample67	sample68	sample69	sample70	sample71	sample72	sample73	sample74	sample75	sample76	sample77	sample78	sample79	sample80	sample81	sample82	sample83	sample84	sample85	sample86	sample87	sample88	sample89	sample90	sample91	sample92	sample93
Glucose	0.26028	6.3624	21.693615	0.27353500000000003	6.266	21.917745	0.381985	6.28287	21.18872	0.20485000000000003	6.0045150000000005	20.3886	0.203645	7.07576	20.3645	0.201235	6.182855000000001	21.782785000000004	0.068685	5.89004	21.657465	0.11086	5.887630000000001	20.13073	0.051815	5.664704999999999	19.33543	0.068685	5.727365	19.586070000000003	0.06266	5.77918	20.797095	0.034945000000000004	4.7693900000000005	19.284820000000003	0.22172	4.62238	19.598119999999998	0.06989000000000001	4.66817	17.49419	0.030125000000000002	4.23919	18.943805	0.06748	4.79108	17.47973	0.06507	3.6282550000000002	17.873765000000002	0.03374	3.6836849999999997	17.853279999999998	0.06507	2.12562	17.471295	0.06989000000000001	3.40292	18.86066	0.066275	0.30125	14.34432	0.3856	0.34462999999999994	13.911724999999999	0.08314500000000001	0.09158	14.792580000000001	0.07109499999999999	0.054224999999999995	13.651445	0.087965	0.18918500000000002	12.61876	0.08917		10.11236	0.09398999999999999		12.118685000000001	0.09881000000000001	0.09398999999999999	7.50715	0.107245		7.201079999999999				0.095195	0.15665	0.654315
Pyruvate	0.06748	0.09881000000000001	0.08676	0.08314500000000001	0.08555499999999999	0.100015	0.08314500000000001	0.090375	0.10363	0.073505	0.078325	0.08435000000000001	0.06748	0.097605	0.09398999999999999	0.075915	0.08435000000000001	0.095195	0.38439500000000004	0.44826	0.442235	0.39765	0.44826	0.437415	0.360295	0.40729	0.41692999999999997	0.372345	0.42054499999999995	0.40729	0.36632	0.451875	0.44826	0.44344	0.87001	0.80012	0.40006	0.844705	0.885675	0.38319000000000003	0.859165	0.5796049999999999	0.41692999999999997	0.690465	0.842295	0.34101499999999996	0.9025449999999999	0.649495	0.38921500000000003	1.01461	0.95918	0.42295499999999997	1.049555	1.013405	0.39524000000000004	0.608525	0.9676150000000001	0.3856	1.03148	1.06763	0.26269	1.170055	1.3255000000000001	0.30727499999999996	1.338755	1.3532149999999998	0.27353500000000003	1.071245	1.4303350000000001	0.28076500000000004	1.049555	1.345985	0.227745	0.94954	1.374905	0.20003	0.5940650000000001	1.05317	0.201235	0.849525	1.341165	0.090375	0.757945	1.14475	0.107245	0.736255	1.08209				0.08194000000000001	0.27474	0.42656999999999995

			volume (start = 4 mL)		time		glucose (new calibration; already corrected for dilution)	pyruvate	glucose		average				stdev
Date	Time	t		1	0	well 1	0.26028	0.06748			0 mM	6 mM	22 mM	volume	0 mM	6 mM	22 mM
160802	1899-12-31T10:0:0+0000	0	-0.4	2	0	well 2	6.3624	0.09881000000000001		0	0.25425500000000006	6.3624	21.222660833333332	4	0.07005186428639844	0.37008608478298644	0.7002754283381417
	16.35	6.5	-0.4	3	0	well 3	21.693615	0.08676		6.5	0.072541	5.789783999999999	20.301358	3.6	0.022501580333389896	0.0990939426377824	0.943205850693526
160803	1899-12-31T9:15:0+0000	23.25	-0.4	4	0	well 4	0.27353500000000003	0.08314500000000001		23.25	0.084832	4.618042000000001	18.560133	3.2	0.07865152323699777	0.2229959859952642	1.006640152360813
	1899-12-31T16:10:0+0000	30.25	-0.4	5	0	well 5	6.266	0.08555499999999999		30.25	0.05844250000000001	3.21012	18.01475	2.8	0.016624342342881817	0.7331240862341573	0.5935364061425936
160804	1899-12-31T9:30:0+0000	47.5	-0.4	6	0	well 6	21.917745	0.100015		47.5	0.15152875000000002	0.19792125	14.175017500000001	2.4	0.1562087037361128	0.14623880198582725	0.5011646041056108
	1899-12-31T16:5:0+0000	54.25	-0.4	7	0	well 7	0.381985	0.08314500000000001		54.25	0.09037499999999998	0.18918500000000002	11.616601666666668	2	0.0031881303298328255		1.32649010369785
160805	1899-12-31T9:20:0+0000	71.5	-0.4	8	0	well 8	6.28287	0.090375		71.5	0.1030275		7.354115	1.6	0.005964445699308517		0.21642417251776727
	1899-12-31T16:10:0+0000	78.25	-0.2	9	0	well 9	21.18872	0.10363		78.25				1.2
160808	1899-12-31T9:45:0+0000	143.75	end of experiment	10	0	well 10	0.20485000000000003	0.073505		143.75	0.095195	0.15665	0.654315	0.8
				11	0	well 11	6.0045150000000005	0.078325
				12	0	well 12	20.3886	0.08435000000000001
				13	0	well 13	0.203645	0.06748
				14	0	well 14	7.07576	0.097605
				15	0	well 15	20.3645	0.09398999999999999
				16	0	well 16	0.201235	0.075915
				17	0	well 17	6.182855000000001	0.08435000000000001
				18	0	well 18	21.782785000000004	0.095195
				19	6.5	well 4	0.068685	0.38439500000000004
				20	6.5	well 5	5.89004	0.44826
				21	6.5	well 6	21.657465	0.442235
				22	6.5	well 7	0.11086	0.39765
				23	6.5	well 8	5.887630000000001	0.44826
				24	6.5	well 9	20.13073	0.437415
				25	6.5	well 10	0.051815	0.360295
				26	6.5	well 11	5.664704999999999	0.40729
				27	6.5	well 12	19.33543	0.41692999999999997
				28	6.5	well 13	0.068685	0.372345
				29	6.5	well 14	5.727365	0.42054499999999995
				30	6.5	well 15	19.586070000000003	0.40729
				31	6.5	well 16	0.06266	0.36632
				32	6.5	well 17	5.77918	0.451875
				33	6.5	well 18	20.797095	0.44826
				34	23.25	well 4	0.034945000000000004	0.44344
				35	23.25	well 5	4.7693900000000005	0.87001
				36	23.25	well 6	19.284820000000003	0.80012
				37	23.25	well 7	0.22172	0.40006
				38	23.25	well 8	4.62238	0.844705
				39	23.25	well 9	19.598119999999998	0.885675
				40	23.25	well 10	0.06989000000000001	0.38319000000000003
				41	23.25	well 11	4.66817	0.859165
				42	23.25	well 12	17.49419	0.5796049999999999
				43	23.25	well 13	0.030125000000000002	0.41692999999999997
				44	23.25	well 14	4.23919	0.690465
				45	23.25	well 15	18.943805	0.842295
				46	23.25	well 16	0.06748	0.34101499999999996
				47	23.25	well 17	4.79108	0.9025449999999999
				48	23.25	well 18	17.47973	0.649495
				49	30.25	well 7	0.06507	0.38921500000000003
				50	30.25	well 8	3.6282550000000002	1.01461
				51	30.25	well 9	17.873765000000002	0.95918
				52	30.25	well 10	0.03374	0.42295499999999997
				53	30.25	well 11	3.6836849999999997	1.049555
				54	30.25	well 12	17.853279999999998	1.013405
				55	30.25	well 13	0.06507	0.39524000000000004
				56	30.25	well 14	2.12562	0.608525
				57	30.25	well 15	17.471295	0.9676150000000001
				58	30.25	well 16	0.06989000000000001	0.3856
				59	30.25	well 17	3.40292	1.03148
				60	30.25	well 18	18.86066	1.06763
				61	47.5	well 7	0.066275	0.26269
				62	47.5	well 8	0.30125	1.170055
				63	47.5	well 9	14.34432	1.3255000000000001
				64	47.5	well 10	0.3856	0.30727499999999996
				65	47.5	well 11	0.34462999999999994	1.338755
				66	47.5	well 12	13.911724999999999	1.3532149999999998
				67	47.5	well 13	0.08314500000000001	0.27353500000000003
				68	47.5	well 14	0.09158	1.071245
				69	47.5	well 15	14.792580000000001	1.4303350000000001
				70	47.5	well 16	0.07109499999999999	0.28076500000000004
				71	47.5	well 17	0.054224999999999995	1.049555
				72	47.5	well 18	13.651445	1.345985
				73	54.25	well 10	0.087965	0.227745
				74	54.25	well 11	0.18918500000000002	0.94954
				75	54.25	well 12	12.61876	1.374905
				76	54.25	well 13	0.08917	0.20003
				77	54.25	well 14		0.5940650000000001
				78	54.25	well 15	10.11236	1.05317
				79	54.25	well 16	0.09398999999999999	0.201235
				80	54.25	well 17		0.849525
				81	54.25	well 18	12.118685000000001	1.341165
				82	71.5	well 13	0.09881000000000001	0.090375
				83	71.5	well 14	0.09398999999999999	0.757945
				84	71.5	well 15	7.50715	1.14475
				85	71.5	well 16	0.107245	0.107245
				86	71.5	well 17		0.736255
				87	71.5	well 18	7.201079999999999	1.08209
				88	78.25	well 16
				89	78.25	well 17
				90	78.25	well 18
				91	143.75	well 16	0.095195	0.08194000000000001
				92	143.75	well 17	0.15665	0.27474
				93	143.5	well 18	0.654315	0.42656999999999995


	1	2	3	4	5	6	7	8	9	10	11	12
A	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
B	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
C
D	13	16	28	31	43	46	55	58	67	70	76	79	und
E	82	85	91
F	13	16	28	31	43	46	55	58	67	70	76	79	2x
G	82	85	91
H


	1	2	3	4	5	6	7	8	9	10	11	12
A	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
B	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
C
D	13	16	28	31	43	46	55	58	67	70	76	79	und
E	82	85	91
F	13	16	28	31	43	46	55	58	67	70	76	79	2x
G	82	85	91
H


Photometric1

Wavelength: 340

Plate: Plate 1

time base		0	0.012166666666666666	0.022166666666666668	0.027666666666666666	0.03983333333333334	0.04533333333333334	3.333333333333333E-4	0.012	0.022000000000000002	0.028	0.0395	0.04566666666666667	6.666666666666666E-4	0.011666666666666665	0.021666666666666667	0.028333333333333332	0.03933333333333333	0.046	0.001	0.011333333333333334	0.021333333333333333	0.039	0.0013333333333333333	0.011000000000000001	0.021	0.03866666666666666	0.0015	0.010666666666666666	0.020666666666666667	0.03833333333333333	0.0018333333333333333	0.010333333333333333	0.02033333333333333	0.038	0.0021666666666666666	0.010166666666666666	0.020166666666666666	0.03766666666666666	0.0025	0.009833333333333333	0.01983333333333333	0.0375	0.0195	0.03716666666666667	0.019166666666666665	0.036833333333333336	0.01883333333333333	0.0365
reading	avg time [min]	A01	B01	D01	E01	F01	G01	A02	B02	D02	E02	F02	G02	A03	B03	D03	E03	F03	G03	A04	B04	D04	F04	A05	B05	D05	F05	A06	B06	D06	F06	A07	B07	D07	F07	A08	B08	D08	F08	A09	B09	D09	F09	D10	F10	D11	F11	D12	F12
1	0	0.2285	0.2352	0.2315	0.236	0.2325	0.2342	0.2272	0.2365	0.2286	0.2325	0.2323	0.2334	0.2274	0.2314	0.231	0.2399	0.2284	0.2322	0.2334	0.2308	0.2305	0.2281	0.233	0.2306	0.2362	0.2268	0.2275	0.2277	0.2284	0.2249	0.2321	0.2244	0.2262	0.2253	0.2301	0.2263	0.2285	0.228	0.2281	0.2391	0.2339	0.2269	0.2312	0.2295	0.2402	0.2324	0.2319	0.2329
2	0.4999826388888887	0.2276	0.234	0.2313	0.2356	0.232	0.2323	0.2261	0.2352	0.2276	0.2319	0.2318	0.2323	0.2262	0.2313	0.2299	0.2389	0.2274	0.2321	0.2324	0.2303	0.2295	0.2279	0.2316	0.2296	0.2351	0.2257	0.2265	0.2278	0.2272	0.2241	0.231	0.2242	0.2262	0.2247	0.2293	0.2256	0.2285	0.2276	0.2282	0.2389	0.2333	0.2256	0.23	0.2291	0.2395	0.232	0.2313	0.2327
3	0.9999826388888893	0.2268	0.2333	0.2315	0.2339	0.2313	0.2313	0.2258	0.235	0.2278	0.2311	0.2312	0.232	0.2259	0.2305	0.2297	0.2386	0.2273	0.2305	0.2316	0.2302	0.2292	0.2272	0.2318	0.2286	0.2353	0.2254	0.2259	0.2274	0.2276	0.223	0.2312	0.2239	0.2261	0.2243	0.2292	0.2255	0.2278	0.2269	0.2268	0.2381	0.2332	0.2262	0.2298	0.2288	0.2391	0.2312	0.231	0.2317
4	1.4999826388888888	0.2259	0.2332	0.2306	0.2341	0.2313	0.2312	0.2258	0.2347	0.2274	0.231	0.2309	0.2318	0.2255	0.2311	0.2304	0.2377	0.2275	0.2308	0.2315	0.2284	0.2294	0.2267	0.2304	0.2294	0.2351	0.225	0.2252	0.227	0.2275	0.2231	0.2297	0.2241	0.226	0.224	0.2286	0.2251	0.2278	0.224	0.226	0.2378	0.2325	0.2255	0.2298	0.228	0.2391	0.231	0.2302	0.2311

	average	0.2272	0.233925	0.231225	0.2349	0.231775	0.23224999999999998	0.226225	0.23535	0.22785	0.231625	0.23155	0.232375	0.22625	0.231075	0.23025	0.23877500000000002	0.22765000000000002	0.2314	0.23222500000000001	0.229925	0.22965000000000002	0.227475	0.23170000000000002	0.22954999999999998	0.235425	0.22572499999999998	0.226275	0.227475	0.22767500000000002	0.22377499999999997	0.231	0.22415	0.226125	0.224575	0.2293	0.225625	0.22815000000000002	0.226625	0.227275	0.238475	0.23322500000000002	0.22605000000000003	0.23020000000000002	0.22885	0.239475	0.23165	0.2311	0.2321


Photometric3

Wavelength: 340

Plate: Plate 1

time base		135.81883333333334	135.831	135.841	135.8465	135.85866666666666	135.86416666666668	135.81916666666666	135.83066666666667	135.84066666666666	135.84683333333334	135.85833333333332	135.8645	135.8195	135.83033333333333	135.84033333333335	135.84716666666665	135.858	135.86483333333334	135.81966666666668	135.83016666666668	135.84016666666668	135.85783333333333	135.82	135.82983333333334	135.83983333333333	135.8575	135.82033333333334	135.8295	135.8395	135.85716666666667	135.82066666666665	135.82916666666668	135.83916666666667	135.85683333333333	135.821	135.82883333333334	135.83883333333333	135.8565	135.82133333333334	135.8285	135.8385	135.85616666666667	135.83833333333334	135.856	135.838	135.85566666666668	135.83766666666668	135.85533333333333
reading	avg time [min]	A01	B01	D01	E01	F01	G01	A02	B02	D02	E02	F02	G02	A03	B03	D03	E03	F03	G03	A04	B04	D04	F04	A05	B05	D05	F05	A06	B06	D06	F06	A07	B07	D07	F07	A08	B08	D08	F08	A09	B09	D09	F09	D10	F10	D11	F11	D12	F12
1	0	0.2215	0.2304	0.7453	0.3032	0.492	0.264	0.2534	0.2667	0.6684	0.3017	0.4914	0.2845	0.2923	0.2997	0.7488	0.2572	0.5119	0.2425	0.3633	0.3618	0.7655	0.502	0.4318	0.4384	0.8092	0.4776	0.491	0.4976	0.7239	0.4774	0.5678	0.5683	0.68	0.4498	0.6334	0.6349	0.7054	0.4758	0.8895	0.9232	0.5523	0.3957	0.5397	0.3915	0.4868	0.352	0.4786	0.3547
2	0.4998576388888675	0.2212	0.2311	0.7459	0.3042	0.4932	0.2644	0.2537	0.2673	0.666	0.3017	0.4915	0.2853	0.2928	0.3	0.7465	0.2578	0.5129	0.2428	0.3623	0.363	0.7668	0.5039	0.4333	0.4386	0.8101	0.478	0.4901	0.4977	0.723	0.4772	0.5668	0.5676	0.6793	0.4496	0.6355	0.6353	0.7056	0.4773	0.8877	0.9237	0.5537	0.3955	0.5399	0.3918	0.4868	0.3532	0.4798	0.3557
3	0.9998576388888826	0.2224	0.2315	0.746	0.3048	0.4922	0.265	0.2536	0.267	0.6658	0.3035	0.4903	0.286	0.2922	0.3006	0.7463	0.258	0.5104	0.2437	0.3633	0.3629	0.7664	0.5039	0.4336	0.4394	0.8124	0.4821	0.4923	0.4969	0.7266	0.4805	0.5682	0.568	0.6803	0.4502	0.635	0.6359	0.7041	0.475	0.8878	0.9228	0.5528	0.3953	0.5406	0.3924	0.4875	0.3537	0.4803	0.3562
4	1.4998541666666674	0.2228	0.2321	0.7452	0.3051	0.4922	0.2649	0.2533	0.2674	0.6653	0.3035	0.4909	0.2859	0.293	0.3	0.746	0.2576	0.5119	0.2439	0.3628	0.363	0.7674	0.5046	0.4333	0.4413	0.812	0.4813	0.4924	0.4999	0.7284	0.4796	0.569	0.5687	0.6802	0.4495	0.6359	0.6347	0.704	0.4754	0.8859	0.9221	0.5513	0.3954	0.5408	0.3932	0.4887	0.3539	0.481	0.3565
5	1.9998576388888827	0.2228	0.2322	0.7454	0.3046	0.4922	0.2649	0.2537	0.2671	0.6656	0.3042	0.4909	0.2855	0.2927	0.2996	0.7471	0.2583	0.5133	0.2431	0.3618	0.3625	0.7688	0.5033	0.433	0.4413	0.8128	0.4776	0.4926	0.5008	0.7258	0.4766	0.5704	0.5683	0.6794	0.4508	0.6363	0.6362	0.7029	0.4784	0.8837	0.9209	0.5524	0.3961	0.5402	0.3927	0.4886	0.3537	0.4815	0.3568
6	2.499854166666667	0.2226	0.2317	0.7448	0.3043	0.4937	0.265	0.254	0.2675	0.6682	0.303	0.4917	0.2852	0.2935	0.3016	0.7483	0.2579	0.5116	0.2433	0.3625	0.364	0.767	0.5029	0.4328	0.4404	0.8101	0.4792	0.4912	0.4986	0.7224	0.4783	0.5677	0.5689	0.6783	0.4503	0.6362	0.6372	0.7055	0.4771	0.8925	0.9274	0.5563	0.396	0.5419	0.3925	0.4869	0.3534	0.4805	0.3562
7	2.9998611111111284	0.2223	0.2311	0.7456	0.3033	0.4933	0.2643	0.2529	0.2668	0.668	0.3027	0.4906	0.285	0.2929	0.3006	0.7492	0.2581	0.513	0.2429	0.3622	0.3632	0.7649	0.5016	0.4333	0.4398	0.8094	0.4788	0.4913	0.4981	0.7243	0.4778	0.5672	0.5685	0.6794	0.4495	0.6351	0.6359	0.7051	0.4769	0.8892	0.9251	0.5558	0.3952	0.5411	0.3917	0.4869	0.3533	0.4802	0.355
8	3.499854166666667	0.2217	0.231	0.7437	0.3045	0.4933	0.2643	0.2536	0.2667	0.6695	0.3018	0.491	0.2842	0.2928	0.3008	0.7488	0.2579	0.512	0.2431	0.3625	0.3627	0.7659	0.5027	0.4332	0.4401	0.8098	0.4786	0.4901	0.4979	0.7238	0.4785	0.5677	0.5678	0.681	0.4508	0.6348	0.6366	0.7037	0.4751	0.8873	0.9236	0.5541	0.3958	0.5417	0.3917	0.487	0.3527	0.4802	0.3558
9	3.9998576388888827	0.2221	0.2316	0.7441	0.3039	0.4924	0.2651	0.2538	0.2675	0.668	0.3023	0.4912	0.2851	0.2931	0.3008	0.7498	0.258	0.5124	0.2429	0.3631	0.3637	0.7665	0.5025	0.4335	0.4394	0.81	0.4792	0.4911	0.4982	0.7234	0.4786	0.5676	0.568	0.6807	0.4498	0.6345	0.6369	0.7049	0.4767	0.8886	0.9252	0.5539	0.3965	0.5408	0.3921	0.4876	0.3534	0.4803	0.3555
10	4.499857638888883	0.2226	0.2322	0.7443	0.3045	0.493	0.265	0.2538	0.2671	0.6684	0.3027	0.4916	0.2852	0.2935	0.3006	0.7494	0.258	0.5131	0.2432	0.3632	0.3628	0.7663	0.5028	0.4332	0.44	0.8094	0.4792	0.4916	0.4987	0.7241	0.4783	0.568	0.5683	0.6808	0.4497	0.635	0.6368	0.7055	0.4769	0.8893	0.926	0.5538	0.396	0.5411	0.3927	0.4875	0.3531	0.4804	0.3555

	average	0.2222	0.23149000000000003	0.74503	0.30424	0.49275000000000013	0.26469	0.25358	0.26711000000000007	0.66732	0.30271000000000003	0.49111000000000005	0.28519	0.29288	0.3004300000000001	0.74802	0.25788	0.5122499999999999	0.24314	0.36269999999999997	0.36296000000000006	0.7665500000000001	0.50302	0.43309999999999993	0.43987	0.8105200000000001	0.47916	0.49137000000000003	0.4984400000000001	0.72457	0.47828	0.56804	0.56824	0.67994	0.45	0.63517	0.63604	0.70467	0.47646	0.8881499999999999	0.9240000000000002	0.5536399999999999	0.39575	0.54078	0.39223	0.48743	0.35324	0.48028000000000004	0.35579


well	calibration	sample	dilution factor	start	end	deltaA	average blank	deltaA -minus blanc	average calibration	calculation with calibration curve	correction for dilution			1	2	average technical	average	stdev
A01	0		1	0.2272	0.2222	-0.0050000000000000044	-0.0037174999999999847	-0.0012825000000000197	0	-0.009178373250000059	-0.009178373250000059	13	0	1.5192730372499996	1.5488211485000007	1.5340470928750003	1.4772131412500003	0.08037534519133147
B01	0		1	0.233925	0.23149000000000003	-0.002434999999999965		0.0012825000000000197		-0.001621626749999942	-0.001621626749999942	16	0	1.30027469375	1.5404836855000001	1.4203791896250002
D01		13	1	0.231225	0.74503	0.513805		0.5175224999999999		1.5192730372499996	1.5192730372499996	28	6.5	1.5309543237500003	1.6880243734999991	1.6094893486249997	1.610240604125	0.0010624357169075264
E01		82	1	0.2349	0.30424	0.06934000000000001		0.0730575		0.20983470075	0.20983470075	31	6.5	1.58731321675	1.6346705025000001	1.610991859625
F01		13	2	0.231775	0.49275000000000013	0.2609750000000001		0.2646925000000001		0.7744105742500004	1.5488211485000007	43	23.25	1.6998395062500005	1.5043939604999998	1.6021167333750002	1.5460966418750002	0.07922437316468187
G01		82	2	0.23224999999999998	0.26469	0.03244		0.03615749999999998		0.10112361074999994	0.20224722149999988	46	23.25	1.4694544862499999	1.5106986144999999	1.490076550375
A02	0.1		1	0.226225	0.25358	0.027355000000000018		0.031072500000000003	0.03327500000000003	0.08614269225	0.08614269225	55	30.25	1.3425364982499997	1.3393534385	1.3409449683749999	1.3936249189999999	0.07450070063901996
B02	0.1		1	0.23535	0.26711000000000007	0.031760000000000066		0.03547750000000005		0.09912026275000015	0.09912026275000015	58	30.25	1.4094276987499998	1.4831820404999998	1.4463048696249998
D02		16	1	0.22785	0.66732	0.43947		0.4431875		1.30027469375	1.30027469375	67	47.5	0.9495267582499997	1.0110105934999998	0.9802686758749997	0.9637152764999999	0.023410041899503
E02		85	1	0.231625	0.30271000000000003	0.07108500000000004		0.07480250000000002		0.21497564525000007	0.21497564525000007	70	47.5	0.9205518647500001	0.9737718895	0.9471618771250001
F02		16	2	0.23155	0.49111000000000005	0.25956		0.2632775		0.7702418427500001	1.5404836855000001	76	54.25	0.7360523522499999	0.7275368514999999	0.7317946018749999	0.7357902499999999	0.00565069976884559
G02		85	2	0.232375	0.28519	0.052815		0.056532499999999986		0.16115039824999997	0.32230079649999993	79	54.25	0.73966132475	0.7399104714999998	0.7397858981249998
A03	0.2		1	0.22625	0.29288	0.06662999999999997		0.07034749999999995	0.07171000000000001	0.20185076974999985	0.20185076974999985	82	71.5	0.20983470075	0.20224722149999988	0.20604096112499992	0.23733959099999996	0.04426294685292084
B03	0.2		1	0.231075	0.3004300000000001	0.06935500000000008		0.07307250000000007		0.2098788922500002	0.2098788922500002	85	71.5	0.21497564525000007	0.32230079649999993	0.268638220875
D03		28	1	0.23025	0.74802	0.5177700000000001		0.5214875000000001		1.5309543237500003	1.5309543237500003	91	143.5	0.0618373672499999	0.08027868149999991	0.0710580243749999	0.0710580243749999
E03		91	1	0.23877500000000002	0.25788	0.019104999999999983		0.022822499999999968		0.0618373672499999	0.0618373672499999
F03		28	2	0.22765000000000002	0.5122499999999999	0.28459999999999985		0.28831749999999984		0.8440121867499996	1.6880243734999991
G03		91	2	0.2314	0.24314	0.01174		0.015457499999999985		0.040139340749999954	0.08027868149999991
A04	0.4		1	0.23222500000000001	0.36269999999999997	0.13047499999999995		0.13419249999999994	0.1354725	0.3899445242499998	0.3899445242499998
B04	0.4		1	0.229925	0.36296000000000006	0.13303500000000007		0.13675250000000005		0.39748654025000013	0.39748654025000013
D04		31	1	0.22965000000000002	0.7665500000000001	0.5369		0.5406175		1.58731321675	1.58731321675
F04		31	2	0.227475	0.50302	0.27554500000000004		0.2792625		0.8173352512500001	1.6346705025000001
A05	0.6		1	0.23170000000000002	0.43309999999999993	0.2013999999999999		0.2051174999999999	0.20957749999999994	0.5988966667499998	0.5988966667499998
B05	0.6		1	0.22954999999999998	0.43987	0.21032		0.2140375		0.62517587875	0.62517587875
D05		43	1	0.235425	0.8105200000000001	0.5750950000000001		0.5788125000000002		1.6998395062500005	1.6998395062500005
F05		43	2	0.22572499999999998	0.47916	0.25343499999999997		0.25715249999999995		0.7521969802499999	1.5043939604999998
A06	0.8		1	0.226275	0.49137000000000003	0.265095		0.2688125	0.27174750000000003	0.7865485062500001	0.7865485062500001
B06	0.8		1	0.227475	0.4984400000000001	0.2709650000000001		0.2746825000000001		0.8038421132500003	0.8038421132500003
D06		46	1	0.22767500000000002	0.72457	0.49689500000000003		0.5006125		1.4694544862499999	1.4694544862499999
F06		46	2	0.22377499999999997	0.47828	0.254505		0.25822249999999997		0.7553493072499999	1.5106986144999999
A07	1		1	0.231	0.56804	0.33704		0.3407575	0.3442825	0.9985056707500001	0.9985056707500001
B07	1		1	0.22415	0.56824	0.34409		0.3478075		1.0192756757499999	1.0192756757499999
D07		55	1	0.226125	0.67994	0.45381499999999997		0.45753249999999995		1.3425364982499997	1.3425364982499997
F07		55	2	0.224575	0.45	0.22542500000000001		0.2291425		0.66967671925	1.3393534385
A08	1.2		1	0.2293	0.63517	0.40587		0.4095875	0.41186	1.2012857337499998	1.2012857337499998
B08	1.2		1	0.225625	0.63604	0.4104150000000001		0.41413250000000007		1.21467575825	1.21467575825
D08		58	1	0.22815000000000002	0.70467	0.47652		0.4802375		1.4094276987499998	1.4094276987499998
F08		58	2	0.226625	0.47646	0.249835		0.25355249999999996		0.7415910202499999	1.4831820404999998
A09	2		1	0.227275	0.8881499999999999	0.6608749999999999		0.6645924999999999	0.6769175000000001	1.9525559642499997	1.9525559642499997
B09	2		1	0.238475	0.9240000000000002	0.6855250000000002		0.6892425000000002		2.025177329250001	2.025177329250001
D09		67	1	0.23322500000000002	0.5536399999999999	0.3204149999999999		0.3241324999999999		0.9495267582499997	0.9495267582499997
F09		67	2	0.22605000000000003	0.39575	0.16969999999999996		0.17341749999999995		0.5055052967499999	1.0110105934999998
D10		70	1	0.23020000000000002	0.54078	0.31058		0.3142975		0.9205518647500001	0.9205518647500001
F10		70	2	0.22885	0.39223	0.16338000000000003		0.1670975		0.48688594475	0.9737718895
D11		76	1	0.239475	0.48743	0.24795499999999998		0.25167249999999997		0.7360523522499999	0.7360523522499999
F11		76	2	0.23165	0.35324	0.12159		0.1253075		0.36376842574999996	0.7275368514999999
D12		79	1	0.2311	0.48028000000000004	0.24918000000000004		0.2528975		0.73966132475	0.73966132475
F12		79	2	0.2321	0.35579	0.12369		0.12740749999999998		0.3699552357499999	0.7399104714999998


	1	2	3	4	5	6	7	8	9	10	11	12
A	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
B	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
C
D	14	17	29	32	44	47	56	59	68	71	77	80	2x
E	83	86	92
F	14	17	29	32	44	47	56	59	68	71	77	80	10x
G	83	86	92
H


	1	2	3	4	5	6	7	8	9	10	11	12
A	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
B	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
C
D	15	18	30	33	45	48	57	60	69	72	78	81	5x
E	84	87	93
F	15	18	30	33	45	48	57	60	69	72	78	81	20x
G	84	87	93
H

AZ(uM)																	concentrations in micromolar
buis nr.	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
monsternr	16	17	18	46	47	48	70	71	72	88	89	90	91	92	93
mM Glc	0	6	22	0	6	22	0	6	22	0	6	22	0	6	22

Taurine	10.1807	9.8632	9.2642	9.3551	8.5176	8.8494	8.4444	6.8414	7.4202	7.9344	3.3279	5.4374	3.3133	22.0629	48.1644
Asparaginezuur	13.6194	13.4369	nd	25.76	21.2321	21.4849	39.6189	27.3365	26.8646	26.7569	22.4817	15.9099	17.8858	21.9506	20.246
Threonine	732.9756	727.8681	696.7656	677.9641	644.1552	679.8405	647.5991	604.9975	608.0441	661.7886	493.2942	491.3665	733.1671	528.5776	458.4927
Serine	380.6859	378.4756	359.6072	291.9269	269.0365	256.5057	261.2216	208.3676	211.9052	138.95	237.2429	130.1062	44.3684	44.8235	36.6591
Asparagine	0.5677	0.3807	0.1958	3.0056	1.1003	1.1417	10.9035	0.7165	0.4436	1.799	0.7234	0.1868	15.4304	20.0348	1.4036
Glutaminezuur	89.5032	88.931	85.8358	228.2609	218.6031	230.0903	447.6887	391.5621	390.3207	421.6781	593.7632	442.1657	291.6187	340.3988	670.6153
Glutamine	1684.4297	1710.6993	1627.6312	921.4737	921.2789	988.2149	54.0743	216.0954	240.6739	3.1675	27.5128	43.4456	1.9604	1.0008	12.8809
Glycine	396.239	393.9561	375.2425	452.2252	396.6003	419.7008	622.2819	442.2986	420.8636	1022.2788	533.8474	307.3895	1339.2296	1228.045	265.0404
Alanine	99.611	101.5143	95.8031	171.1694	277.2352	279.618	134.9547	546.3139	525.7481	3.9647	186.4986	220.246	5.6084	4.1282	74.0526
Valine	687.6505	682.607	651.9628	580.5883	546.5355	582.9848	473	431.0286	440.1588	364.602	173.5152	188.4942	199.0469	12.6158	60.0643
Cystine	162.9779	160.2093	153.0764	99.7602	87.8479	94.0822	28.1632	25.3963	26.4067	12.3389	2.2704	3.0204	9.3029	3.2677	1.8433
Methionine	150.9868	149.8856	143.7462	133.212	124.8206	133.1767	113.2069	96.5167	99.1895	95.4116	45.5323	45.4362	87.6464	22.7555	21.8699
iso-leucine	703.74	697.9167	667.8349	510.359	486.5653	522.0996	381.9939	359.9437	369.7776	275.7557	122.4195	139.1587	119.1116	7.4155	49.023
leucine	675.879	673.8586	644.5804	500.9583	471.6263	505.2324	362.1059	320.3925	329.9112	245.0172	85.8279	99.7179	109.0597	4.5655	31.4141
Tyrosine	341.1117	341.0458	326.0552	329.0714	316.5417	333.5043	339.8101	343.3313	342.3413	385.4504	361.9037	356.0293	360.7809	278.0419	227.2412
Phenylalanine	365.2238	362.1475	348.0481	318.1543	296.1883	315.951	259.8835	206.9931	216.7817	170.8241	13.4258	24.6703	140.9912	22.4192	4.6006
Ornithine	21.2333	20.2352	19.2125	34.8739	33.0794	34.4005	60.5219	53.7486	52.8663	77.9394	52.1555	46.7029	32.318	12.3108	9.3696
Lysine	696.2848	690.1495	658.898	629.3763	593.2349	628.6294	577.9418	517.6346	527.9209	584.4462	253.6406	295.9309	662.6358	123.9453	102.9468
Histidine	180.4602	177.3049	169.7171	160.4519	150.719	159.9232	146.2743	129.3153	130.8706	143.4049	85.1158	82.7732	163.7249	93.2939	69.5736
Arginine	335.0133	330.3411	318.3322	277.4295	260.0258	276.1226	207.1983	169.4179	174.1671	111.5341	21.6963	22.1184	25.4222	8.7402	4.4079

som AZ	7728.373499999999	7710.826399999999	7351.809199999998	6355.375999999999	6124.9439	6471.552899999998	5176.8869	5098.248100000001	5142.6757	4755.0425000000005	3316.1950999999995	2960.306	4362.622600000001	2800.393500000001	2169.9093

Int.St.(Area)	107.8672	107.2806	109.5705	109.5795	111.5321	108.6379	108.743	107.8505	108.7785	103.09	107.5078	108.3262	107.2167	106.9579	106.456






buis nr.	1	4	7	10	13		2	5	8	11	14		3	6	9	12	15
monsternr	16	46	70	88	91		17	47	71	89	92		18	48	72	90	93
mM Glc	0	0	0	0	0		6	6	6	6	6		22	22	22	22	22
time	0	23.25	47.5	78.25	143.75		0	23.25	47.5	78.25	143.75		0	23.25	47.5	78.25	143.75
Taurine	10.1807	9.3551	8.4444	7.9344	3.3133		9.8632	8.5176	6.8414	3.3279	22.0629		9.2642	8.8494	7.4202	5.4374	48.1644
Asparaginezuur	13.6194	25.76	39.6189	26.7569	17.8858		13.4369	21.2321	27.3365	22.4817	21.9506		nd	21.4849	26.8646	15.9099	20.246
Threonine	732.9756	677.9641	647.5991	661.7886	733.1671		727.8681	644.1552	604.9975	493.2942	528.5776		696.7656	679.8405	608.0441	491.3665	458.4927
Serine	380.6859	291.9269	261.2216	138.95	44.3684		378.4756	269.0365	208.3676	237.2429	44.8235		359.6072	256.5057	211.9052	130.1062	36.6591
Asparagine	0.5677	3.0056	10.9035	1.799	15.4304		0.3807	1.1003	0.7165	0.7234	20.0348		0.1958	1.1417	0.4436	0.1868	1.4036
Glutaminezuur	89.5032	228.2609	447.6887	421.6781	291.6187		88.931	218.6031	391.5621	593.7632	340.3988		85.8358	230.0903	390.3207	442.1657	670.6153
Glutamine	1684.4297	921.4737	54.0743	3.1675	1.9604		1710.6993	921.2789	216.0954	27.5128	1.0008		1627.6312	988.2149	240.6739	43.4456	12.8809
Glycine	396.239	452.2252	622.2819	1022.2788	1339.2296		393.9561	396.6003	442.2986	533.8474	1228.045		375.2425	419.7008	420.8636	307.3895	265.0404
Alanine	99.611	171.1694	134.9547	3.9647	5.6084		101.5143	277.2352	546.3139	186.4986	4.1282		95.8031	279.618	525.7481	220.246	74.0526
Valine	687.6505	580.5883	473	364.602	199.0469		682.607	546.5355	431.0286	173.5152	12.6158		651.9628	582.9848	440.1588	188.4942	60.0643
Cystine	162.9779	99.7602	28.1632	12.3389	9.3029		160.2093	87.8479	25.3963	2.2704	3.2677		153.0764	94.0822	26.4067	3.0204	1.8433
Methionine	150.9868	133.212	113.2069	95.4116	87.6464		149.8856	124.8206	96.5167	45.5323	22.7555		143.7462	133.1767	99.1895	45.4362	21.8699
iso-leucine	703.74	510.359	381.9939	275.7557	119.1116		697.9167	486.5653	359.9437	122.4195	7.4155		667.8349	522.0996	369.7776	139.1587	49.023
leucine	675.879	500.9583	362.1059	245.0172	109.0597		673.8586	471.6263	320.3925	85.8279	4.5655		644.5804	505.2324	329.9112	99.7179	31.4141
Tyrosine	341.1117	329.0714	339.8101	385.4504	360.7809		341.0458	316.5417	343.3313	361.9037	278.0419		326.0552	333.5043	342.3413	356.0293	227.2412
Phenylalanine	365.2238	318.1543	259.8835	170.8241	140.9912		362.1475	296.1883	206.9931	13.4258	22.4192		348.0481	315.951	216.7817	24.6703	4.6006
Ornithine	21.2333	34.8739	60.5219	77.9394	32.318		20.2352	33.0794	53.7486	52.1555	12.3108		19.2125	34.4005	52.8663	46.7029	9.3696
Lysine	696.2848	629.3763	577.9418	584.4462	662.6358		690.1495	593.2349	517.6346	253.6406	123.9453		658.898	628.6294	527.9209	295.9309	102.9468
Histidine	180.4602	160.4519	146.2743	143.4049	163.7249		177.3049	150.719	129.3153	85.1158	93.2939		169.7171	159.9232	130.8706	82.7732	69.5736
Arginine	335.0133	277.4295	207.1983	111.5341	25.4222		330.3411	260.0258	169.4179	21.6963	8.7402		318.3322	276.1226	174.1671	22.1184	4.4079

som AZ	7728.373499999999	6355.375999999999	5176.8869	4755.0425000000005	4362.622600000001		7710.826399999999	6124.9439	5098.248100000001	3316.1950999999995	2800.393500000001		7351.809199999998	6471.552899999998	5142.6757	2960.306	2169.9093

Int.St.(Area)	107.8672	109.5795	108.743	103.09	107.2167		107.2806	111.5321	107.8505	107.5078	106.9579		109.5705	108.6379	108.7785	108.3262	106.456



buis	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30
id	22	23	24	34	35	36	37	38	39	40	41	42	49	50	51	52	53	54	55	56	57	61	62	63	64	65	66	67	68	69
Condition	6.5 0	6,5 6	6,5 22	23,25 0	23,25 6	23,25 22	23,25 0	23,25 6	23,25 22	23,25 0	23,25 6	23,25 22	30,25 0	30,25 6	30,25 22	30,25 0	30,25 6	30,25 22	30,25 0	30,25 6	30,25 22	47,5 0	47,5 6	47,5 22	47,5 0	47,5 6	47,5 22	47,5 0	47,5 6	47,5 22

Taurine	11.2608	11.3438	10.6076	9.8186	9.5994	9.3577	9.7003	9.7533	9.0074	9.6533	9.3414	9.4994	9.4651	8.7396	7.906	9.8576	8.3077	8.6921	9.4172	8.4667	7.7867	8.7645	7.4201	6.788	9.1839	6.3842	7.4888	8.9504	7.3689	7.817
Asparaginezuur	19.0952	18.0907	17.5281	26.6532	23.5015	23.0091	27.7597	23.3526	22.5111	26.2898	22.7706	22.9386	35.2094	24.2591	24.1277	32.7079	24.8192	24.6219	32.3421	24.8132	22.9794	42.1774	27.8141	27.6195	41.2797	26.9063	26.1203	39.9903	27.6497	27.3561
Threonine	703.326	713.7484	687.4195	678.1282	671.153	675.3753	655.8859	662.9528	648.2123	662.5754	650.8373	666.6019	661.3436	635.605	628.3965	671.1056	640.2824	656.6454	645.6852	647.5492	621.5381	634.6409	577.6035	567.9828	622.9382	599.2471	564.2976	624.961	588.8449	584.9444
Serine	344.908	352.6755	339.7453	293.7642	282.0093	285.9215	279.9933	278.5829	270.9819	288.2859	277.8159	281.7001	267.1323	249.1376	240.8244	277.2982	257.5413	260.0938	264.6021	256.3328	243.3432	252.9779	193.5092	178.2669	251.1782	206.5614	195.4059	252.7411	203.6991	204.8431
Asparagine	1.1015	1.6352	1.5141	3.9213	2.1475	2.3648	3.8949	2.1298	2.4837	5.1342	1.9573	1.5361	8.621	2.1633	1.7684	8.1715	2.7169	5.3816	7.6075	3.0781	1.8043	8.4416	1.4539	0.8448	8.0201	0.97	0.548	8.3546	1.2326	2.6792
Glutaminezuur	125.1285	133.3436	126.6985	232.2359	234.4781	229.8506	242.3353	237.4932	233.5109	231.8182	226.9564	234.8932	315.3694	278.8762	275.9712	300.7904	272.2775	284.2533	293.6994	276.9519	268.6944	467.4895	388.3383	389.8068	445.7	394.4766	375.2481	447.4555	384.9895	389.5048
Glutamine	1458.2557	1527.3911	1452.4778	966.7634	1026.237	1041.6801	895.4687	1011.6303	971.3889	934.6915	991.2166	989.4678	639.7001	781.1319	751.3184	702.2629	794.5065	786.2516	656.1152	762.4117	748.2136	34.0832	243.61	211.0253	60.5955	267.2221	230.8372	49.7879	237.965	251.8547
Proline	31.2716	30.5292	28.6738	66.3283	72.9472	71.4092	66.4008	73.3525	76.6388	65.1177	72.7405	75.1462	89.1647	92.2665	89.6582	88.6732	95.0631	97.9256	83.9086	100.279	88.2682	131.0063	170.2871	171.8429	122.4267	170.1371	170.1258	128.1504	171.59	168.5523
Glycine	403.6958	407.0671	388.5657	455.0155	420.6053	420.5804	450.1344	415.419	407.319	445.7366	408.6804	418.2545	493.5739	414.7003	411.6311	490.5966	416.4721	426.3126	474.7207	421.8705	403.7684	627.9838	420.1199	414.8119	604.767	443.9301	401.3504	605.6211	430.9833	411.1417
Alanine	121.7458	133.5911	125.3393	178.3295	289.2436	272.0986	179.2682	288.1144	274.0886	177.6049	288.3913	288.4434	200.262	373.93	361.3768	205.2564	381.7948	383.2019	193.543	379.5585	350.8455	117.0191	543.0364	531.6013	152.6853	582.3001	529.4826	137.4774	549.036	521.6934
Citrulline	8.8386	9.4988	7.2554	7.2294	7.8273	7.6491	8.0583	8.2372	8.3252	6.4979	8.0967	7.4092	7.8739	8.3841	8.05	7.9598	7.4981	8.0163	7.902	7.6228	7.4928	6.5798	6.8843	6.3402	7.048	6.5003	6.2643	5.7479	6.7519	6.3126
a-a-boterzuur	3.2863	3.5404	1.9995	2.3986	2.6296	2.7879	2.8157	3.5805	2.547	2.5385	2.8094	2.834	2.7875	3.4519	2.7956	2.8977	2.6866	3.6245	2.2777	3.4102	2.6532	2.8383	2.4642	2.1066	2.6557	3.2978	1.8109	2.547	2.6991	2.5694
Valine	665.1382	677.0509	647.9079	602.9946	591.5993	602.7144	579.1393	589.7618	576.5195	589.4967	577.4208	589.6567	560.3939	544.5334	538.1437	577.0043	550.7421	561.207	550.6385	553.4688	534.1131	472.8859	430.0916	428.3084	477.0759	453.8895	424.0524	472.9201	437.9748	441.6006
Cystine	162.1526	163.4662	157.8489	113.6828	105.2651	109.2205	106.4917	103.1369	100.2609	110.143	104.0928	105.723	79.9177	79.7212	76.8943	90.1599	82.6527	83.2612	84.508	84.1626	78.9647	28.4452	30.5527	28.3726	32.3161	34.9236	29.8608	30.9721	32.3055	30.8141
Methionine	148.8356	154.3366	146.1233	139.0605	136.1024	138.6849	134.5698	135.1055	132.493	136.2905	133.8989	136.7645	130.3082	125.8099	124.0293	135.2499	128.3967	129.7545	127.5201	127.7313	123.7774	114.8173	98.094	100.4878	118.6958	104.5549	97.7686	114.7245	101.9988	102.2174
iso-leucine	632.6653	644.9379	622.5964	524.4665	521.7699	533.6104	500.2953	517.6831	507.4262	510.0162	508.9983	521.1826	470.1152	470.3376	464.4854	484.562	474.5532	485.1908	461.6952	477.0608	462.2198	377.5764	361.4318	364.2658	386.2841	378.8368	360.0459	382.0914	370.8198	370.2201
leucine	619.8315	624.049	601.9586	512.0194	502.5734	514.5756	488.863	500.0351	489.8429	498.2437	490.4331	502.0084	457.297	447.9905	440.3021	470.1134	451.5195	460.1933	449.8292	454.237	439.7779	353.912	320.3343	323.1012	366.0054	332.4423	319.1386	359.8843	330.475	330.2935
Tyrosine	348.1103	351.3231	338.3626	342.8224	341.2665	341.129	333.7786	340.308	329.61	334.1064	337.3201	340.3999	342.8394	338.6974	324.2586	343.2051	341.9193	341.8451	332.0004	341.0859	326.4309	352.307	335.4611	338.0932	346.8822	348.5438	332.4002	347.4809	342.8211	341.131
Phenylalanine	342.4589	350.3148	336.3198	319.0746	310.3356	315.7247	306.7904	307.4923	300.5687	308.0859	301.0194	307.5003	297.2068	282.3009	276.3695	305.3302	283.4504	288.9822	291.1704	285.0571	275.2571	248.6525	205.5511	203.0638	253.585	215.4615	200.8057	248.2637	207.8135	207.5384
Ornithine	25.5002	25.6671	24.073	35.8986	35.3891	34.9063	36.9642	34.8199	34.3926	36.0875	34.453	35.2551	44.3974	38.6863	38.3116	43.2346	39.2933	39.8899	40.4288	39.2719	37.0654	63.5582	51.5508	50.8955	60.0621	53.1579	49.7349	60.1209	52.4527	51.9337
Lysine	674.3832	682.1575	658.3626	641.841	631.0919	639.2682	621.7269	622.9778	609.4606	626.8811	613.5243	627.0999	618.8471	593.7414	583.7372	627.8546	599.824	610.1174	602.8565	602.8282	578.4904	574.883	510.0433	510.2182	577.3947	538.6436	508.2099	572.6847	523.0354	523.4259
Histidine	173.1984	177.2416	169.3585	163.6909	160.8889	163.0811	158.4946	158.2402	155.2619	158.5865	155.7137	158.9676	155.3089	149.9719	146.1532	158.5721	151.8689	154.0722	152.6863	151.617	145.7459	144.5263	125.9496	126.593	144.7944	133.4153	124.8362	143.4633	128.577	129.8574
Tryptofaan	81.51696863459648	88.27521421793541	81.51026630619666	78.9336819759929	78.93308321960934	77.11297831639557	77.14729742767574	75.7202690542833	72.52051913493598	75.12858793000672	73.69579193292559	74.73088318063226	73.06060760374945	72.2559239702292	67.7508610674535	76.80674446429356	73.4837087853523	73.69606628240734	75.01416528893276	72.26489504089389	71.19396426152987	68.27825519563517	57.9202763615631	60.529189343713426	72.38599807479437	62.91703982888095	59.04279389526062	65.95424910332997	59.09992033999559	64.46440081368824
Arginine	318.3268	323.5389	312.4474	285.1931	277.7121	283.8089	273.0373	275.6877	266.7242	277.8224	271.3015	276.4071	259.2999	248.7742	243.1481	267.7187	252.1791	257.7783	257.5901	252.0209	244.021	201.714	168.5104	163.6478	205.9801	177.3104	165.5399	201.3971	173.608	171.106

Dutch	English
Taurine	Taurine
Asparaginezuur	Aspartic acid
Threonine	Threonine
Serine	Serine
Asparagine	Asparagine
Glutaminezuur	Glutamate
Glutamine	Glutamine
Proline	Proline
Glycine	Glycine
Alanine	Alanine
Citrulline	Citrulline
a-a-boterzuur	Butyric acid
Valine	Valine
Cystine	Cystine
Methionine	Methionine
iso-leucine	iso-leucine
leucine	leucine
Tyrosine	Tyrosine
Phenylalanine	Phenylalanine
Ornithine	Ornithine
Lysine	Lysine
Histidine	Histidine
Tryptofaan	Tryptophan
Arginine	Arginine


Photometric2

Wavelength: 340

Plate: Plate 1

time base		6.2523333333333335	6.2645	6.274500000000001	6.28	6.292166666666666	6.297666666666667	6.2524999999999995	6.264166666666667	6.274166666666667	6.280166666666667	6.291833333333333	6.298	6.252833333333333	6.263833333333333	6.273833333333333	6.2805	6.2915	6.298166666666666	6.253166666666667	6.2635	6.2735	6.291166666666667	6.2535	6.263333333333334	6.273166666666667	6.2909999999999995	6.253833333333334	6.263	6.273	6.290666666666667	6.254166666666666	6.262666666666666	6.272666666666667	6.290333333333334	6.254333333333333	6.262333333333333	6.272333333333333	6.29	6.254666666666666	6.2620000000000005	6.272	6.289666666666666	6.2716666666666665	6.289333333333333	6.271333333333333	6.289	6.271166666666667	6.288833333333333
reading	avg time [min]	A01	B01	D01	E01	F01	G01	A02	B02	D02	E02	F02	G02	A03	B03	D03	E03	F03	G03	A04	B04	D04	F04	A05	B05	D05	F05	A06	B06	D06	F06	A07	B07	D07	F07	A08	B08	D08	F08	A09	B09	D09	F09	D10	F10	D11	F11	D12	F12
1	0	0.2281	0.2343	0.3859	0.264	0.3181	0.2397	0.2425	0.2352	0.3571	0.2639	0.3099	0.2503	0.2552	0.237	0.3851	0.2494	0.2998	0.2377	0.2822	0.2445	0.3816	0.3007	0.3107	0.2567	0.3594	0.3084	0.3248	0.2697	0.3572	0.2868	0.3361	0.272	0.3604	0.2932	0.381	0.2837	0.3868	0.2847	0.4302	0.3184	0.3275	0.2777	0.3303	0.2637	0.3231	0.2622	0.3169	0.273
2	0.4999097222222209	0.2281	0.2353	0.3931	0.265	0.3234	0.241	0.2433	0.2406	0.3655	0.2598	0.3185	0.2541	0.2572	0.244	0.3906	0.2495	0.3062	0.2376	0.2849	0.2554	0.3937	0.3064	0.3135	0.2728	0.3668	0.3117	0.3276	0.2881	0.3674	0.2925	0.3414	0.292	0.3611	0.3098	0.3865	0.3025	0.3941	0.2923	0.4483	0.3412	0.3327	0.2823	0.3312	0.2689	0.3241	0.2672	0.3185	0.2764
3	0.9999097222222209	0.2274	0.2352	0.4001	0.2661	0.3282	0.2417	0.2428	0.2437	0.3712	0.2607	0.3251	0.257	0.257	0.2493	0.3962	0.2496	0.3125	0.2381	0.2863	0.264	0.401	0.3131	0.315	0.2844	0.3736	0.3179	0.3304	0.3019	0.3753	0.2983	0.3454	0.306	0.3688	0.3186	0.3893	0.3179	0.4028	0.299	0.4471	0.3567	0.3372	0.2879	0.3381	0.2734	0.3255	0.2711	0.3234	0.2821
4	1.4999062499999998	0.2266	0.2342	0.4075	0.2669	0.3347	0.2428	0.2428	0.2463	0.3794	0.2625	0.3314	0.2588	0.2566	0.2526	0.4036	0.2495	0.3163	0.2381	0.288	0.2716	0.4072	0.32	0.3162	0.2942	0.381	0.3234	0.3359	0.3143	0.3828	0.3048	0.3497	0.3192	0.376	0.3251	0.393	0.3315	0.4115	0.3048	0.4527	0.3738	0.343	0.2948	0.3424	0.2765	0.3291	0.2742	0.3286	0.2873
5	1.9999097222222229	0.2262	0.2343	0.4156	0.267	0.3401	0.2432	0.2419	0.248	0.384	0.2638	0.3366	0.2583	0.2568	0.2555	0.4112	0.2489	0.3212	0.2385	0.2881	0.2771	0.4134	0.326	0.3196	0.3021	0.3884	0.3299	0.3411	0.3236	0.3891	0.3087	0.354	0.3292	0.3843	0.3319	0.3973	0.3421	0.4216	0.3115	0.4648	0.3874	0.3486	0.2998	0.3469	0.2797	0.3327	0.2777	0.3343	0.291
6	2.4999097222222235	0.2259	0.2331	0.4217	0.2682	0.3456	0.2438	0.2422	0.2505	0.3896	0.2659	0.3402	0.2595	0.2574	0.2579	0.4178	0.2493	0.3262	0.2378	0.2885	0.2821	0.42	0.3318	0.3234	0.3096	0.3946	0.3374	0.3468	0.3331	0.3971	0.3137	0.3588	0.3389	0.3954	0.3394	0.4033	0.3524	0.432	0.3177	0.4756	0.4	0.3544	0.3039	0.3515	0.2835	0.3387	0.2804	0.3389	0.2947
7	2.9999097222222235	0.2243	0.2319	0.4275	0.2667	0.3472	0.2432	0.2424	0.2523	0.3951	0.2657	0.3454	0.2598	0.2575	0.2591	0.4231	0.2492	0.3303	0.2377	0.289	0.2874	0.4259	0.3366	0.3271	0.3153	0.4013	0.3434	0.3516	0.3408	0.4055	0.3179	0.3635	0.349	0.4059	0.3459	0.4079	0.3634	0.4409	0.3229	0.4816	0.4101	0.3581	0.3068	0.3554	0.2859	0.3431	0.2826	0.342	0.296
8	3.4999097222222235	0.2249	0.2323	0.4355	0.2677	0.3502	0.2444	0.2428	0.254	0.4028	0.2675	0.3519	0.2619	0.2591	0.2623	0.4301	0.2492	0.3355	0.2381	0.2908	0.2922	0.4335	0.3416	0.3296	0.3213	0.4084	0.3495	0.3561	0.3495	0.4132	0.3211	0.3699	0.3552	0.4152	0.3517	0.4152	0.3727	0.4523	0.3287	0.4932	0.4222	0.3632	0.309	0.3615	0.2898	0.348	0.2857	0.347	0.2999
9	3.9999097222222235	0.2236	0.2317	0.4399	0.2675	0.3533	0.2453	0.2421	0.2538	0.4081	0.268	0.3561	0.2617	0.2589	0.2631	0.4358	0.2487	0.3385	0.2374	0.2928	0.2966	0.44	0.3452	0.3305	0.3257	0.4147	0.3544	0.3577	0.3566	0.4191	0.3249	0.3734	0.3596	0.4216	0.3545	0.4207	0.3815	0.4596	0.3336	0.502	0.4304	0.3666	0.3101	0.3649	0.2917	0.3516	0.2874	0.3518	0.3021
10	4.499909722222224	0.2226	0.2306	0.4452	0.2685	0.357	0.245	0.2419	0.2551	0.4122	0.2686	0.3589	0.2621	0.2591	0.2651	0.4418	0.2483	0.3414	0.2365	0.2929	0.2994	0.4457	0.349	0.3328	0.3297	0.4199	0.3594	0.361	0.3641	0.4248	0.3279	0.377	0.3657	0.4278	0.358	0.4246	0.3905	0.4683	0.3375	0.5076	0.4405	0.3706	0.3121	0.3694	0.293	0.3553	0.2889	0.3565	0.3047
11	4.99990625	0.2219	0.2311	0.4508	0.2685	0.3617	0.2452	0.2414	0.2567	0.4168	0.2699	0.3613	0.2636	0.2591	0.2672	0.449	0.2491	0.3453	0.2371	0.2932	0.3028	0.4509	0.3525	0.3347	0.3342	0.4261	0.3664	0.3654	0.3725	0.4318	0.3322	0.3818	0.3727	0.4321	0.3568	0.4306	0.3992	0.4755	0.3407	0.5153	0.4518	0.3741	0.3144	0.3732	0.2948	0.3595	0.2911	0.362	0.3074
12	5.499909722222224	0.2216	0.2299	0.455	0.2681	0.365	0.2452	0.2417	0.2568	0.4218	0.2704	0.3633	0.263	0.2589	0.2685	0.4548	0.2482	0.3481	0.2364	0.2948	0.3059	0.4563	0.3561	0.3359	0.338	0.432	0.3713	0.3682	0.3802	0.4371	0.3348	0.3847	0.3765	0.4378	0.359	0.4358	0.4072	0.4821	0.3445	0.5213	0.4615	0.3777	0.317	0.3756	0.2956	0.3622	0.2932	0.3649	0.3096
13	5.999909722222226	0.2208	0.229	0.4594	0.2681	0.368	0.2444	0.2409	0.2573	0.4255	0.2708	0.3658	0.2625	0.2587	0.2709	0.4612	0.2478	0.3506	0.2351	0.2961	0.3081	0.4623	0.3593	0.3372	0.3417	0.437	0.3758	0.3694	0.39	0.4407	0.3363	0.3874	0.3827	0.443	0.3591	0.4408	0.4164	0.4889	0.3472	0.5273	0.4693	0.3809	0.3191	0.3808	0.2969	0.366	0.2949	0.3683	0.3113
14	6.499909722222222	0.22	0.2289	0.4652	0.2672	0.372	0.2446	0.2398	0.2578	0.4307	0.2703	0.3692	0.2636	0.2587	0.2732	0.4664	0.2477	0.3536	0.2356	0.2967	0.3109	0.4682	0.3631	0.3409	0.3449	0.442	0.3798	0.3724	0.3963	0.4463	0.3401	0.3915	0.3891	0.4475	0.3637	0.4465	0.4271	0.4948	0.3502	0.5334	0.4777	0.3833	0.3223	0.3841	0.2987	0.3697	0.2961	0.3727	0.3137
15	6.999909722222222	0.2203	0.229	0.4719	0.2683	0.376	0.2449	0.2414	0.2583	0.4355	0.2712	0.3724	0.2641	0.2593	0.2755	0.4724	0.248	0.3565	0.235	0.2979	0.3132	0.4737	0.3662	0.3412	0.3489	0.4466	0.3831	0.3761	0.4021	0.4517	0.343	0.3965	0.3962	0.4534	0.3672	0.4521	0.4373	0.5016	0.3535	0.5397	0.4854	0.387	0.3253	0.3861	0.301	0.3722	0.2984	0.377	0.3162
16	7.499909722222222	0.2195	0.2287	0.4806	0.2692	0.3795	0.2454	0.2417	0.2581	0.4403	0.2726	0.3742	0.2646	0.2599	0.2759	0.4781	0.247	0.3594	0.2355	0.2991	0.3151	0.4794	0.3694	0.3419	0.3508	0.4513	0.388	0.3773	0.4074	0.4581	0.3455	0.4011	0.4026	0.4589	0.3705	0.4602	0.4455	0.5065	0.3563	0.5464	0.4927	0.3907	0.3271	0.3888	0.3021	0.3758	0.2991	0.3807	0.3197
17	7.999909722222222	0.2191	0.2283	0.487	0.269	0.383	0.2449	0.2415	0.2583	0.4427	0.2726	0.3769	0.2645	0.2599	0.2769	0.4842	0.2472	0.3611	0.2349	0.2993	0.3165	0.4833	0.3724	0.3408	0.3536	0.4556	0.3904	0.3781	0.4113	0.4635	0.3468	0.4029	0.4075	0.4646	0.3743	0.466	0.452	0.5121	0.3586	0.5546	0.5004	0.3933	0.3275	0.3922	0.3033	0.3799	0.3001	0.3854	0.3218
18	8.499916666666664	0.2192	0.2282	0.4928	0.27	0.3863	0.2453	0.2413	0.2584	0.4464	0.2728	0.3799	0.2653	0.2609	0.2774	0.4905	0.2476	0.3644	0.2354	0.3003	0.3177	0.4834	0.3762	0.3418	0.3559	0.4589	0.393	0.3793	0.4164	0.4686	0.3488	0.4065	0.4129	0.4688	0.3759	0.471	0.4595	0.5179	0.3604	0.5641	0.5093	0.3961	0.3299	0.3962	0.3044	0.3825	0.3009	0.3891	0.3242
19	8.99990625	0.2194	0.2285	0.4989	0.2698	0.3899	0.2451	0.2414	0.259	0.4502	0.2733	0.3812	0.2654	0.2607	0.2782	0.4954	0.247	0.3666	0.2351	0.3024	0.3191	0.4904	0.379	0.3423	0.3588	0.4634	0.3952	0.3825	0.4201	0.4721	0.352	0.4085	0.419	0.4729	0.3783	0.4737	0.4663	0.5231	0.3628	0.5734	0.5159	0.3977	0.3306	0.4004	0.3056	0.385	0.3014	0.3917	0.3257
20	9.499909722222219	0.2184	0.2273	0.5108	0.2706	0.3931	0.2448	0.2416	0.2583	0.4536	0.2734	0.3846	0.2651	0.2608	0.2786	0.5004	0.247	0.3692	0.235	0.3035	0.3206	0.4946	0.3808	0.3428	0.3601	0.4677	0.3975	0.3838	0.4228	0.4759	0.3527	0.4101	0.4262	0.4797	0.3812	0.4765	0.4725	0.5281	0.3652	0.5819	0.5229	0.4005	0.3335	0.4031	0.3067	0.388	0.3025	0.395	0.3265
21	9.999909722222219	0.2177	0.2273	0.5121	0.2702	0.3964	0.2449	0.2408	0.2582	0.457	0.2739	0.3871	0.265	0.2607	0.2793	0.5054	0.2469	0.3712	0.234	0.3039	0.3213	0.4984	0.3827	0.3435	0.3616	0.4712	0.4	0.3871	0.4261	0.4765	0.3541	0.4121	0.4338	0.4828	0.3835	0.4798	0.4789	0.5313	0.3673	0.5896	0.529	0.4017	0.3347	0.4057	0.3069	0.3892	0.3029	0.3965	0.3274
22	10.499909722222219	0.2179	0.2275	0.517	0.2722	0.3996	0.246	0.2421	0.2598	0.4607	0.2751	0.3896	0.2664	0.2619	0.2797	0.5097	0.2467	0.3737	0.2349	0.3051	0.3234	0.504	0.3859	0.3457	0.3646	0.4775	0.4045	0.3892	0.4293	0.4815	0.357	0.4176	0.4405	0.4862	0.3873	0.4826	0.4856	0.5361	0.3707	0.5966	0.5366	0.4053	0.3374	0.4092	0.3089	0.3931	0.3046	0.4004	0.3293
23	10.999909722222215	0.2183	0.2266	0.5244	0.2736	0.4032	0.2461	0.2425	0.2587	0.4636	0.2754	0.3929	0.2667	0.2624	0.2807	0.514	0.2472	0.3759	0.2351	0.3061	0.324	0.5089	0.3886	0.3484	0.3665	0.4815	0.4065	0.3936	0.4316	0.4857	0.3589	0.4202	0.4461	0.4897	0.3903	0.4865	0.491	0.5409	0.3732	0.6033	0.543	0.4071	0.3393	0.4121	0.3117	0.3958	0.3063	0.4026	0.3304
24	11.499909722222215	0.2173	0.2274	0.5325	0.2729	0.4053	0.2448	0.2422	0.2589	0.4661	0.2756	0.3939	0.266	0.2615	0.2803	0.5185	0.2463	0.3784	0.2342	0.3057	0.3243	0.5123	0.391	0.3487	0.3672	0.4843	0.4085	0.3981	0.4325	0.489	0.36	0.4236	0.4502	0.4932	0.3921	0.4894	0.4953	0.5436	0.3745	0.6087	0.5487	0.4097	0.3436	0.4145	0.3122	0.3984	0.307	0.4046	0.3312
25	11.999909722222215	0.2172	0.2263	0.5365	0.2732	0.4078	0.2451	0.2422	0.2594	0.4688	0.2762	0.3965	0.2666	0.2625	0.281	0.5223	0.2463	0.3798	0.2336	0.3063	0.3255	0.5179	0.3932	0.3504	0.3688	0.4879	0.4106	0.4035	0.4348	0.4947	0.3617	0.4275	0.4541	0.4992	0.3945	0.4927	0.4991	0.5473	0.3766	0.6157	0.5542	0.4115	0.346	0.4175	0.3142	0.3999	0.3078	0.4081	0.3329
26	12.499909722222215	0.2166	0.2264	0.5411	0.2741	0.4077	0.2454	0.2429	0.2596	0.4722	0.2763	0.3973	0.2666	0.2627	0.2814	0.5267	0.2466	0.3806	0.2338	0.3073	0.326	0.5215	0.3954	0.3518	0.3701	0.4916	0.4137	0.4057	0.4369	0.4983	0.3644	0.4307	0.4582	0.5034	0.3977	0.4968	0.5025	0.5507	0.3787	0.6218	0.559	0.4133	0.3473	0.4214	0.3157	0.4031	0.3082	0.4113	0.3345
27	12.999906249999997	0.2168	0.2261	0.5476	0.2751	0.4143	0.2456	0.2424	0.2589	0.4759	0.2769	0.4005	0.2674	0.2629	0.2817	0.53	0.2468	0.3827	0.2341	0.3076	0.3273	0.5248	0.3975	0.3537	0.3715	0.4957	0.4157	0.4066	0.4384	0.5046	0.3658	0.4336	0.462	0.5096	0.3998	0.501	0.5055	0.5551	0.3795	0.6275	0.5635	0.4154	0.3471	0.4229	0.3173	0.4038	0.3095	0.4122	0.335
28	13.499909722222219	0.2164	0.226	0.5492	0.2752	0.4117	0.2449	0.2421	0.2592	0.4796	0.277	0.4004	0.267	0.263	0.2813	0.535	0.246	0.3841	0.2332	0.3088	0.3273	0.5272	0.3985	0.3563	0.3728	0.5002	0.418	0.4091	0.4393	0.5069	0.3673	0.4343	0.4642	0.5117	0.4019	0.5053	0.5078	0.5592	0.3813	0.6335	0.5688	0.4184	0.3479	0.4251	0.317	0.4065	0.3101	0.4145	0.336
29	13.999906249999997	0.2162	0.2261	0.5511	0.2762	0.4156	0.245	0.2426	0.2596	0.4821	0.2775	0.4024	0.268	0.2633	0.282	0.5389	0.2466	0.3856	0.2336	0.3098	0.329	0.5317	0.4002	0.363	0.3746	0.5045	0.4199	0.4104	0.4422	0.508	0.3678	0.4357	0.4679	0.514	0.4039	0.5098	0.5109	0.5637	0.3832	0.639	0.5745	0.421	0.3504	0.4277	0.3174	0.4081	0.312	0.4163	0.3371
30	14.499906249999997	0.2163	0.2256	0.5559	0.2758	0.4164	0.2459	0.2428	0.2597	0.4861	0.2783	0.4044	0.268	0.2633	0.2825	0.5421	0.2464	0.3881	0.2336	0.3106	0.3299	0.5349	0.4022	0.3676	0.3753	0.5081	0.4227	0.4118	0.444	0.5103	0.3694	0.4359	0.4703	0.5165	0.4058	0.5146	0.5134	0.5681	0.3853	0.6445	0.58	0.4214	0.352	0.4313	0.3174	0.4103	0.312	0.4179	0.3377
31	14.99990625	0.2163	0.2252	0.5637	0.2763	0.4198	0.2465	0.2427	0.2596	0.4894	0.2779	0.4056	0.2684	0.2644	0.2828	0.5455	0.2461	0.3892	0.2334	0.3115	0.33	0.5389	0.4034	0.3695	0.3765	0.5109	0.4256	0.4125	0.4457	0.5125	0.3717	0.4355	0.4738	0.5193	0.4081	0.5187	0.5154	0.5717	0.3869	0.6507	0.5853	0.4231	0.3545	0.4336	0.318	0.4123	0.3124	0.4204	0.3381


Photometric2

Wavelength: 340

Plate: Plate 1

time base		6.843833333333333	6.856166666666667	6.866166666666667	6.8715	6.883833333333333	6.889166666666667	6.844166666666666	6.855833333333334	6.865833333333333	6.871833333333333	6.8835	6.8895	6.8445	6.8555	6.8655	6.872166666666667	6.883166666666667	6.889833333333333	6.844833333333333	6.855166666666666	6.865166666666667	6.882833333333334	6.845166666666667	6.854833333333334	6.864833333333333	6.882499999999999	6.8455	6.8545	6.8645000000000005	6.8821666666666665	6.845666666666667	6.854333333333333	6.864333333333334	6.882000000000001	6.846	6.854	6.864	6.881666666666666	6.846333333333333	6.853666666666667	6.863666666666666	6.881333333333333	6.863333333333333	6.881	6.8629999999999995	6.8806666666666665	6.862666666666667	6.880333333333334
reading	avg time [min]	A01	B01	D01	E01	F01	G01	A02	B02	D02	E02	F02	G02	A03	B03	D03	E03	F03	G03	A04	B04	D04	F04	A05	B05	D05	F05	A06	B06	D06	F06	A07	B07	D07	F07	A08	B08	D08	F08	A09	B09	D09	F09	D10	F10	D11	F11	D12	F12
1	0	0.2363	0.2341	0.2951	0.4005	0.2472	0.2677	0.2503	0.2338	0.311	0.3633	0.2477	0.2532	0.2583	0.2371	0.2984	0.2342	0.2485	0.2282	0.2844	0.2355	0.27	0.2377	0.3013	0.2436	0.4058	0.2573	0.3041	0.2427	0.3422	0.2518	0.2989	0.2569	0.3584	0.2551	0.3203	0.2674	0.3429	0.2677	0.4077	0.3012	0.4451	0.2651	0.4628	0.2719	0.4522	0.2788	0.4416	0.2814
2	0.49994791666666366	0.2363	0.2339	0.2994	0.4126	0.2475	0.2748	0.2514	0.237	0.3189	0.3678	0.2495	0.2584	0.2616	0.2416	0.302	0.2341	0.2515	0.2296	0.2882	0.2397	0.2731	0.2398	0.3061	0.2533	0.4195	0.2631	0.3003	0.2525	0.3498	0.256	0.3007	0.2695	0.3686	0.2597	0.3233	0.2759	0.3538	0.2728	0.4121	0.3172	0.4684	0.2724	0.4888	0.2797	0.4724	0.2882	0.4555	0.2899
3	0.9999513888888885	0.2357	0.2347	0.3037	0.4254	0.2487	0.2806	0.2515	0.2385	0.3199	0.378	0.2506	0.2626	0.2651	0.2441	0.304	0.2341	0.2531	0.2291	0.292	0.2432	0.2741	0.2401	0.3056	0.26	0.4305	0.2672	0.3048	0.2593	0.3558	0.2591	0.305	0.2787	0.3767	0.2631	0.3265	0.285	0.3665	0.2775	0.4137	0.3309	0.4719	0.2779	0.5102	0.2843	0.4919	0.2946	0.4714	0.2951
4	1.4999513888888885	0.2351	0.2344	0.3059	0.4332	0.2495	0.2855	0.2519	0.2406	0.3212	0.3897	0.2522	0.2667	0.2626	0.2467	0.3083	0.2339	0.2555	0.2293	0.2941	0.2454	0.2758	0.2411	0.3041	0.2653	0.4411	0.2709	0.3108	0.2645	0.3638	0.2619	0.3092	0.2862	0.3846	0.267	0.3319	0.2932	0.3794	0.2821	0.4187	0.3444	0.4917	0.2847	0.528	0.2887	0.5053	0.3017	0.4845	0.2996
5	1.9999513888888893	0.2348	0.2339	0.3069	0.4431	0.2496	0.2888	0.2509	0.2416	0.321	0.3989	0.2526	0.2705	0.2617	0.2479	0.3126	0.2334	0.257	0.2283	0.2936	0.2469	0.2781	0.2415	0.3051	0.2694	0.4475	0.2742	0.3128	0.2693	0.3708	0.2633	0.3147	0.292	0.3929	0.2705	0.3364	0.2998	0.3848	0.2857	0.427	0.3576	0.51	0.2888	0.5462	0.2921	0.5257	0.3066	0.4958	0.3048
6	2.4999513888888893	0.2333	0.2332	0.3085	0.4531	0.2502	0.2924	0.2505	0.2415	0.3228	0.4076	0.2536	0.2735	0.2623	0.2497	0.3165	0.2328	0.2572	0.2278	0.2948	0.2483	0.2798	0.2413	0.3086	0.2726	0.4518	0.2755	0.3168	0.2729	0.3779	0.2648	0.318	0.2976	0.4006	0.2731	0.3398	0.3058	0.3881	0.2896	0.4366	0.369	0.5321	0.2923	0.5667	0.2953	0.5417	0.3113	0.5048	0.3091
7	2.9999513888888893	0.233	0.2327	0.3084	0.463	0.2506	0.2965	0.2509	0.2422	0.324	0.415	0.2534	0.2761	0.2625	0.2498	0.3202	0.2321	0.2577	0.2275	0.2975	0.2497	0.2815	0.2412	0.3135	0.2763	0.4586	0.2771	0.3222	0.2767	0.3843	0.2666	0.3201	0.3023	0.408	0.2749	0.3425	0.3126	0.394	0.2927	0.4407	0.3799	0.5573	0.2957	0.5839	0.2989	0.5493	0.315	0.5139	0.3142
8	3.4999479166666663	0.2326	0.2322	0.3114	0.4738	0.2507	0.2995	0.2504	0.2429	0.3258	0.422	0.2541	0.2781	0.2616	0.2501	0.3234	0.2319	0.2577	0.2267	0.2977	0.2501	0.2836	0.2419	0.3156	0.2796	0.4667	0.2785	0.3218	0.2793	0.3884	0.2673	0.3239	0.3073	0.4149	0.2767	0.3459	0.3187	0.3988	0.2957	0.4444	0.3911	0.5732	0.2975	0.5945	0.3021	0.5649	0.3176	0.5278	0.3175
9	3.9999513888888893	0.2315	0.2319	0.3172	0.4831	0.2504	0.3011	0.2508	0.2431	0.3286	0.4293	0.255	0.2804	0.263	0.2519	0.3274	0.2314	0.2589	0.2272	0.2976	0.2509	0.2855	0.2419	0.3171	0.2825	0.4742	0.2798	0.3241	0.2821	0.3954	0.2686	0.3284	0.3125	0.4224	0.2772	0.3495	0.324	0.403	0.2982	0.4514	0.3988	0.58	0.3004	0.6036	0.3045	0.5832	0.3203	0.5435	0.3208
10	4.499947916666667	0.2316	0.2309	0.3216	0.4885	0.2502	0.3026	0.2505	0.2428	0.33	0.4359	0.2549	0.2828	0.2625	0.2526	0.3302	0.2312	0.2589	0.2269	0.2986	0.2527	0.2881	0.2426	0.3189	0.2851	0.4792	0.2809	0.3278	0.2855	0.4016	0.2694	0.331	0.3163	0.4285	0.2778	0.3534	0.3285	0.409	0.3	0.4579	0.4084	0.5921	0.3019	0.6158	0.3069	0.5926	0.3215	0.559	0.3213
11	4.999951388888889	0.2309	0.2308	0.3244	0.4974	0.2506	0.3061	0.2502	0.2434	0.3326	0.4435	0.2554	0.2844	0.2629	0.2539	0.3325	0.231	0.2601	0.2257	0.2984	0.2543	0.2891	0.2428	0.3205	0.2882	0.4859	0.283	0.3297	0.2883	0.404	0.2703	0.3346	0.3203	0.4341	0.2796	0.3556	0.334	0.4157	0.3019	0.4654	0.4194	0.6094	0.3045	0.6335	0.3101	0.6059	0.3249	0.5688	0.3249
12	5.499951388888891	0.2301	0.2301	0.3251	0.5056	0.2501	0.3067	0.2511	0.2426	0.3344	0.4501	0.2559	0.2863	0.2622	0.254	0.3355	0.2304	0.2597	0.2249	0.2991	0.2554	0.2914	0.2425	0.3211	0.29	0.4923	0.2829	0.3314	0.2909	0.4077	0.2702	0.3382	0.3242	0.4383	0.2809	0.3587	0.3385	0.4222	0.3031	0.4704	0.427	0.6201	0.3061	0.6522	0.3109	0.6154	0.3273	0.5812	0.3301
13	5.999951388888888	0.2296	0.2298	0.328	0.5148	0.2498	0.3091	0.2502	0.2428	0.3369	0.4567	0.2564	0.2873	0.2627	0.2543	0.3389	0.2297	0.26	0.2248	0.3002	0.2562	0.2928	0.2427	0.324	0.292	0.4984	0.2838	0.3373	0.293	0.4127	0.272	0.3403	0.3273	0.4447	0.2812	0.3618	0.3428	0.4297	0.3044	0.4765	0.4345	0.6293	0.3086	0.6668	0.3135	0.6251	0.3304	0.5917	0.3341
14	6.499951388888888	0.2287	0.2288	0.3306	0.5242	0.2489	0.31	0.2503	0.2426	0.3392	0.4643	0.2563	0.2889	0.2627	0.2553	0.3404	0.23	0.2598	0.2244	0.3008	0.2575	0.294	0.2431	0.3265	0.2936	0.5039	0.2842	0.3382	0.2949	0.4182	0.2719	0.3437	0.3304	0.4494	0.2826	0.3643	0.346	0.4356	0.3066	0.4819	0.4423	0.6379	0.3109	0.6731	0.315	0.6381	0.3324	0.59	0.3382
15	6.999951388888888	0.229	0.2292	0.3345	0.5317	0.2492	0.3115	0.2504	0.2433	0.3412	0.4708	0.2575	0.2922	0.2636	0.2565	0.3426	0.2298	0.261	0.2243	0.302	0.2588	0.2969	0.2434	0.3295	0.2963	0.5117	0.2857	0.3393	0.2981	0.4227	0.2731	0.3458	0.3349	0.4555	0.2847	0.3684	0.3509	0.4394	0.3089	0.4888	0.4509	0.6532	0.3136	0.6912	0.3177	0.6488	0.3349	0.6075	0.341
16	7.499951388888888	0.2284	0.2285	0.3372	0.539	0.2499	0.3137	0.2504	0.243	0.3437	0.4775	0.2572	0.2937	0.2636	0.2574	0.3444	0.2297	0.2607	0.2244	0.3028	0.2596	0.299	0.244	0.332	0.2983	0.5195	0.2867	0.3404	0.2983	0.4257	0.2735	0.3487	0.3384	0.46	0.2864	0.3719	0.354	0.4446	0.3095	0.4941	0.4574	0.6653	0.3156	0.7028	0.3195	0.6592	0.3383	0.6388	0.3431
17	7.999951388888888	0.2283	0.2276	0.3398	0.5464	0.2499	0.3156	0.2496	0.2428	0.3455	0.4847	0.2577	0.2945	0.2652	0.2569	0.3479	0.2294	0.2613	0.2243	0.3033	0.2606	0.3014	0.2442	0.3352	0.3	0.5262	0.2873	0.3442	0.3026	0.4317	0.2733	0.3505	0.3406	0.4661	0.2875	0.3756	0.3581	0.4494	0.311	0.5043	0.4629	0.6767	0.3178	0.7076	0.3214	0.6759	0.3403	0.6517	0.3478
18	8.499951388888887	0.2279	0.2269	0.3428	0.5534	0.2502	0.3161	0.25	0.2429	0.3476	0.4909	0.2584	0.2959	0.2652	0.2581	0.3492	0.229	0.2604	0.2237	0.3042	0.2624	0.3023	0.2452	0.3371	0.3014	0.5337	0.2877	0.3463	0.3052	0.4382	0.2746	0.3528	0.3429	0.4723	0.289	0.3769	0.3613	0.4546	0.3122	0.51	0.4681	0.6886	0.3193	0.7136	0.3233	0.6898	0.3428	0.6576	0.3514
19	8.999951388888883	0.2273	0.2267	0.3447	0.5603	0.2491	0.3178	0.2498	0.2429	0.3495	0.4974	0.2579	0.2967	0.2665	0.258	0.3519	0.2274	0.2604	0.2227	0.304	0.2624	0.3048	0.2451	0.3395	0.3033	0.54	0.2869	0.3487	0.3084	0.4386	0.2744	0.3544	0.3465	0.477	0.29	0.3772	0.3647	0.4593	0.3126	0.5139	0.4729	0.7004	0.322	0.7275	0.3244	0.7013	0.3453	0.6699	0.3542
20	9.499951388888883	0.2275	0.2263	0.3473	0.5675	0.25	0.3198	0.2504	0.2437	0.3516	0.5056	0.2583	0.299	0.2668	0.2593	0.3548	0.2289	0.2609	0.2232	0.306	0.2641	0.3067	0.2453	0.342	0.3049	0.5462	0.289	0.3518	0.3114	0.4449	0.2761	0.3569	0.3504	0.482	0.2922	0.3796	0.3686	0.465	0.3144	0.5184	0.4777	0.7121	0.3243	0.7402	0.3264	0.7076	0.3486	0.6771	0.3576
21	9.999951388888883	0.2269	0.2261	0.3494	0.5739	0.2499	0.3216	0.2506	0.2432	0.354	0.5101	0.2579	0.3002	0.2677	0.2597	0.3565	0.2282	0.2612	0.2222	0.3065	0.2646	0.3098	0.2457	0.3435	0.3062	0.552	0.2891	0.3538	0.3142	0.4514	0.277	0.3578	0.3527	0.4863	0.2934	0.3814	0.3712	0.4686	0.3155	0.5236	0.4821	0.7249	0.3255	0.7501	0.3285	0.7228	0.3512	0.6916	0.3616
22	10.499951388888883	0.2266	0.226	0.3514	0.5808	0.2505	0.3221	0.2509	0.2426	0.3559	0.5166	0.2579	0.3019	0.2683	0.26	0.3589	0.2278	0.2612	0.2221	0.3072	0.2649	0.3123	0.2462	0.3447	0.3082	0.5605	0.2898	0.3543	0.3153	0.4544	0.2779	0.3589	0.3565	0.4904	0.294	0.3847	0.3741	0.474	0.3167	0.5285	0.4868	0.7346	0.3281	0.7568	0.3306	0.737	0.3551	0.6957	0.3657
23	10.999951388888883	0.2259	0.226	0.353	0.5894	0.2507	0.3227	0.251	0.2429	0.3579	0.5234	0.2584	0.3037	0.2687	0.2609	0.3618	0.2277	0.2618	0.2217	0.3084	0.2662	0.3126	0.2466	0.346	0.3094	0.5676	0.2905	0.3541	0.3167	0.4596	0.2787	0.3606	0.3598	0.4955	0.2962	0.3877	0.3771	0.4789	0.3179	0.5351	0.492	0.7472	0.331	0.7701	0.3319	0.745	0.3579	0.7139	0.3712
24	11.499951388888883	0.2264	0.2263	0.3543	0.5965	0.2501	0.3238	0.2509	0.2427	0.3595	0.5294	0.2581	0.3043	0.2688	0.2612	0.363	0.2278	0.2621	0.2218	0.3093	0.266	0.3147	0.2465	0.3474	0.3109	0.5735	0.2918	0.3537	0.3181	0.4662	0.2787	0.3632	0.3626	0.5002	0.298	0.3894	0.3798	0.4842	0.3182	0.5419	0.4965	0.7593	0.3332	0.7822	0.3333	0.7589	0.3598	0.7153	0.3761
25	11.999951388888887	0.2258	0.2258	0.3553	0.6041	0.2498	0.3248	0.2507	0.2428	0.3609	0.5345	0.2588	0.3059	0.2697	0.2609	0.3656	0.2276	0.2624	0.2222	0.3103	0.2665	0.3182	0.2476	0.3477	0.3124	0.5815	0.2922	0.3538	0.321	0.4729	0.2793	0.3657	0.3658	0.5052	0.2994	0.3917	0.3828	0.4884	0.3196	0.5457	0.5022	0.7679	0.3351	0.7958	0.3353	0.7666	0.3628	0.7349	0.378
26	12.499951388888887	0.2255	0.2256	0.3559	0.6115	0.2497	0.3259	0.2503	0.2421	0.3634	0.5392	0.259	0.3073	0.2696	0.2617	0.367	0.2276	0.2626	0.2214	0.3105	0.2679	0.3195	0.2473	0.35	0.3142	0.5881	0.2932	0.3539	0.3227	0.4771	0.2803	0.369	0.3692	0.5091	0.3004	0.3943	0.3859	0.4914	0.3202	0.5461	0.5079	0.7794	0.3373	0.8102	0.3357	0.7818	0.3664	0.7563	0.3772
27	12.999951388888887	0.2258	0.2248	0.3578	0.6183	0.2503	0.3277	0.2512	0.2428	0.3652	0.5459	0.2589	0.3089	0.2709	0.2618	0.3697	0.2272	0.2633	0.221	0.3119	0.2673	0.3204	0.2481	0.3519	0.3155	0.5948	0.2938	0.3568	0.3245	0.4818	0.2814	0.3715	0.3712	0.514	0.3025	0.3958	0.389	0.4964	0.3217	0.5454	0.5139	0.79	0.3397	0.8245	0.3369	0.7936	0.3703	0.7614	0.3809
28	13.499951388888887	0.2256	0.2247	0.3593	0.6264	0.2511	0.3298	0.2515	0.2424	0.3669	0.5515	0.2591	0.3106	0.2715	0.2622	0.3718	0.2277	0.2638	0.2221	0.3134	0.269	0.322	0.2483	0.3532	0.3165	0.6007	0.2936	0.3576	0.3272	0.4877	0.2813	0.3734	0.3739	0.519	0.3033	0.3983	0.3918	0.5015	0.3203	0.5499	0.5193	0.8019	0.3426	0.8386	0.3389	0.8048	0.3726	0.7651	0.3872
29	13.999951388888887	0.2251	0.2244	0.3602	0.632	0.2518	0.3324	0.2514	0.2428	0.369	0.5566	0.2587	0.3106	0.2716	0.2623	0.3734	0.2271	0.2634	0.2209	0.3136	0.2696	0.3238	0.2485	0.3544	0.3182	0.602	0.2931	0.3596	0.3283	0.4913	0.2822	0.3759	0.3751	0.5227	0.3044	0.4001	0.394	0.5064	0.3199	0.5506	0.5244	0.8099	0.3447	0.8478	0.3415	0.8148	0.3754	0.7738	0.3877
30	14.499951388888887	0.225	0.2241	0.3615	0.64	0.2524	0.3348	0.2521	0.2433	0.371	0.5632	0.2584	0.3126	0.2727	0.2625	0.3759	0.2274	0.264	0.2211	0.3148	0.2701	0.326	0.2495	0.355	0.3206	0.6098	0.2954	0.3613	0.3294	0.4969	0.2827	0.377	0.3773	0.5279	0.3058	0.4024	0.398	0.5091	0.322	0.5561	0.5305	0.8185	0.3471	0.862	0.3425	0.8205	0.3799	0.7882	0.3897
31	14.999951388888887	0.2248	0.2239	0.3627	0.6467	0.2522	0.3362	0.2523	0.243	0.3727	0.5676	0.2587	0.3132	0.2729	0.2632	0.3777	0.2269	0.2641	0.2208	0.3161	0.2705	0.3281	0.2496	0.3562	0.3229	0.616	0.2959	0.363	0.3322	0.5022	0.2834	0.38	0.3795	0.5321	0.3075	0.4043	0.4012	0.5135	0.3232	0.5595	0.5351	0.8285	0.3489	0.8732	0.3445	0.8287	0.3816	0.7933	0.3905


Photometric3

Wavelength: 340

Plate: Plate 1

time base		136.41066666666669	136.42283333333333	136.43283333333335	136.43833333333333	136.4505	136.456	136.411	136.42266666666666	136.43266666666668	136.43866666666665	136.45033333333333	136.45633333333333	136.41133333333335	136.42233333333334	136.43233333333333	136.439	136.45	136.45666666666665	136.41166666666666	136.422	136.432	136.44966666666667	136.412	136.42166666666668	136.43166666666667	136.44933333333333	136.41216666666665	136.42133333333334	136.43133333333333	136.44899999999998	136.4125	136.421	136.43099999999998	136.44866666666667	136.41283333333334	136.42083333333332	136.43083333333334	136.4485	136.41316666666665	136.4205	136.4305	136.44816666666668	136.43016666666668	136.44783333333334	136.42983333333333	136.44750000000002	136.42950000000002	136.44716666666667
reading	avg time [min]	A01	B01	D01	E01	F01	G01	A02	B02	D02	E02	F02	G02	A03	B03	D03	E03	F03	G03	A04	B04	D04	F04	A05	B05	D05	F05	A06	B06	D06	F06	A07	B07	D07	F07	A08	B08	D08	F08	A09	B09	D09	F09	D10	F10	D11	F11	D12	F12
1	0	0.2305	0.2304	0.4415	1.2502	0.2742	0.4404	0.2654	0.2629	0.4872	1.2213	0.2775	0.4238	0.3075	0.3088	0.5414	0.2361	0.2895	0.2279	0.3619	0.3648	0.5564	0.2879	0.428	0.4291	0.8322	0.3366	0.4989	0.4977	0.7684	0.3322	0.539	0.6124	1.0439	0.3852	0.6265	0.6326	1.0948	0.3887	0.899	0.9088	1.6736	0.5048	1.8023	0.5286	1.65	0.5155	1.5937	0.5067
2	0.49997569444447737	0.2306	0.23	0.4412	1.2503	0.274	0.4401	0.2655	0.264	0.4873	1.2218	0.2776	0.4234	0.3078	0.3085	0.5409	0.2356	0.2889	0.2277	0.3618	0.3645	0.5563	0.2873	0.4277	0.4289	0.8289	0.3363	0.4998	0.497	0.7685	0.3319	0.5395	0.6116	1.0435	0.3858	0.6267	0.6314	1.0942	0.388	0.8993	0.9088	1.6739	0.5038	1.8046	0.5282	1.6522	0.5155	1.5954	0.506
3	0.9999756944444774	0.2311	0.2305	0.4419	1.2512	0.2747	0.441	0.2653	0.2639	0.488	1.2225	0.2776	0.4245	0.3086	0.3094	0.5411	0.2371	0.2896	0.2279	0.3619	0.3652	0.5575	0.2876	0.4283	0.4296	0.8296	0.3371	0.5006	0.498	0.7693	0.3326	0.5396	0.6123	1.0442	0.3854	0.6271	0.6316	1.0958	0.3885	0.9002	0.9094	1.6758	0.5042	1.8059	0.5288	1.6543	0.5155	1.5979	0.5074
4	1.4999756944444773	0.2302	0.2308	0.4416	1.2511	0.2739	0.441	0.2652	0.2631	0.4882	1.2236	0.2779	0.4242	0.3078	0.3085	0.5413	0.2365	0.2893	0.2275	0.3617	0.3651	0.5569	0.2866	0.4276	0.4297	0.8286	0.337	0.4999	0.4975	0.7691	0.3327	0.5402	0.6124	1.0433	0.3853	0.6272	0.6316	1.0951	0.3875	0.8995	0.9099	1.6753	0.5042	1.8056	0.5287	1.6537	0.5151	1.5984	0.5064
5	1.9999756944444773	0.2314	0.2306	0.442	1.2511	0.2745	0.4411	0.2656	0.2641	0.4884	1.2238	0.2784	0.4241	0.309	0.3089	0.5415	0.2371	0.2903	0.228	0.362	0.3651	0.5562	0.2876	0.4287	0.4301	0.8294	0.3374	0.5005	0.4983	0.7693	0.3329	0.54	0.6126	1.044	0.3857	0.6283	0.6326	1.0964	0.3891	0.9006	0.9108	1.6758	0.5046	1.8062	0.5292	1.6545	0.5157	1.6001	0.5067
6	2.4999756944444775	0.231	0.2301	0.4415	1.2504	0.2743	0.4405	0.2655	0.2644	0.4888	1.2235	0.2778	0.4239	0.308	0.3089	0.5411	0.2366	0.2894	0.2281	0.3627	0.3652	0.5566	0.2876	0.4283	0.4297	0.8285	0.3368	0.5002	0.4982	0.7686	0.3325	0.5397	0.6126	1.0438	0.3849	0.6278	0.6321	1.0956	0.3887	0.9003	0.9097	1.6754	0.5039	1.8099	0.5285	1.653	0.5156	1.5994	0.5067
7	2.999972222222262	0.2309	0.2311	0.4419	1.2502	0.2741	0.44	0.2657	0.2644	0.4883	1.2242	0.2775	0.4236	0.3083	0.3094	0.5412	0.237	0.2892	0.2277	0.3619	0.365	0.5572	0.2882	0.428	0.43	0.8289	0.337	0.5004	0.4983	0.7688	0.3324	0.5399	0.6135	1.0445	0.3858	0.6274	0.6325	1.0958	0.3887	0.9006	0.9104	1.6765	0.5038	1.8087	0.5286	1.6531	0.5156	1.6	0.5066
8	3.4999756944444775	0.2309	0.2307	0.4416	1.2501	0.2744	0.44	0.2655	0.264	0.4881	1.2238	0.2771	0.4238	0.3082	0.309	0.5413	0.2362	0.2893	0.2275	0.362	0.3658	0.5562	0.2876	0.4284	0.4303	0.8291	0.3367	0.4997	0.498	0.7693	0.3327	0.5394	0.6152	1.0441	0.3855	0.6275	0.6317	1.0963	0.3885	0.9003	0.9096	1.6774	0.5045	1.808	0.5282	1.6527	0.5154	1.5998	0.5067
9	3.9999756944444775	0.2312	0.2304	0.4415	1.2511	0.2742	0.4402	0.2657	0.2638	0.4877	1.2237	0.2779	0.4238	0.3081	0.3088	0.5407	0.237	0.2895	0.2275	0.3618	0.3651	0.557	0.2876	0.428	0.4304	0.829	0.3368	0.4995	0.498	0.7695	0.3328	0.5396	0.6159	1.0444	0.3856	0.6266	0.6321	1.0954	0.3891	0.9001	0.9099	1.6771	0.5043	1.8084	0.5279	1.6545	0.5147	1.6001	0.5067
10	4.499979166666693	0.2312	0.2307	0.4416	1.2516	0.2742	0.4403	0.2654	0.2645	0.4886	1.2256	0.2778	0.4241	0.3084	0.3093	0.5419	0.2373	0.2904	0.228	0.3627	0.3652	0.5578	0.2879	0.4285	0.4304	0.8296	0.337	0.5001	0.4981	0.7695	0.3327	0.5401	0.6144	1.0443	0.386	0.6275	0.6319	1.0961	0.3888	0.9001	0.9096	1.6785	0.5045	1.8096	0.5281	1.6526	0.5154	1.5998	0.5069

		0.23089999999999997	0.23053000000000004	0.44162999999999997	1.2507300000000001	0.27425	0.44046	0.26548	0.26391	0.48806000000000005	1.2233800000000001	0.27771	0.42392	0.30816999999999994	0.30895	0.54124	0.23664999999999997	0.28954	0.22778	0.36204000000000003	0.36510000000000004	0.5568100000000001	0.28759	0.4281499999999999	0.42982	0.8293799999999999	0.33687	0.4999599999999999	0.49790999999999996	0.76903	0.33254	0.5397	0.6132899999999999	1.044	0.38552	0.6272599999999999	0.6320100000000001	1.0955499999999998	0.38855999999999996	0.8999999999999998	0.90969	1.67593	0.50426	1.8069199999999999	0.52848	1.65306	0.5154	1.5984599999999998	0.50668


well	calibration	sample	dilution factor	start	end	deltaA	average blank	deltaA -minus blanc	average calibration	calculation with calibration curve	correction for dilution			2	10	average tech	average bio	stdev
A01	0		1	0.23450000000000001	0.23089999999999997	-0.0036000000000000476	-0.0030475000000000085	-5.525000000000391E-4	0	-0.013725123500000115	-0.013725123500000115	14	0	1.2537059369999999	1.2988873149999995	1.2762966259999997	1.3882242495000001	0.1582895631578901
B01	0		1	0.233025	0.23053000000000004	-0.0024949999999999695		5.525000000000391E-4		-0.010474876499999885	-0.010474876499999885	17	0	1.537139241	1.4631645050000008	1.5001518730000005
D01		14	2	0.22744999999999999	0.44162999999999997	0.21417999999999998		0.2172275		0.6268529684999999	1.2537059369999999	29	6.5	1.8489570550000005	1.8133381750000008	1.8311476150000008	1.8634588940000008	0.045695048979420935
E01		83	2	0.23259999999999997	1.2507300000000001	1.0181300000000002		1.0211775000000003		2.991591498500001	5.983182997000002	32	6.5	1.9414346710000008	1.850105675000001	1.8957701730000007
F01		14	10	0.229025	0.27425	0.04522499999999999		0.048272499999999996		0.12988873149999997	1.2988873149999995	44	23.25	3.555645576999999	3.4136068450000003	3.4846262109999997	3.3579621735	0.17912999969743446
G01		83	10	0.22949999999999998	0.44046	0.21096000000000004		0.21400750000000004		0.6173816605000001	6.173816605000001	47	23.25	3.215325596999999	3.2472706749999998	3.2312981359999995
A02	0.1		1	0.232825	0.26548	0.03265499999999999		0.0357025	0.035629999999999995	0.09291533349999999	0.09291533349999999	56	30.25	4.8276245929999995	4.808565794999999	4.818095194	4.910455153999999	0.13061670805223596
B02	0.1		1	0.2314	0.26391	0.03250999999999998		0.03555749999999999		0.09248883049999997	0.09248883049999997	59	30.25	5.1083812229999985	4.897249004999998	5.002815113999999
D02		17	2	0.22569999999999998	0.48806000000000005	0.26236000000000004		0.2654075		0.7685696205	1.537139241	68	47.5	8.516316676999999	8.186469555000002	8.351393116	8.7235022765	0.5262418214623655
E02		86	2	0.23920000000000002	1.2233800000000001	0.98418		0.9872275		2.8917309685	5.783461937	71	47.5	9.263667588999999	8.927555284999997	9.095611436999999
F02		17	10	0.2269	0.27771	0.05081000000000002		0.05385750000000003		0.14631645050000008	1.4631645050000008	77	54.25	8.363687430999999	8.496493115	8.430090273	8.251253152999999	0.25291388055974573
G02		86	10	0.226075	0.42392	0.19784500000000002		0.20089250000000003		0.5788051995000001	5.788051995000001	80	54.25	8.021749680999998	8.123082384999998	8.072416032999998
A03	0.2		1	0.230725	0.30816999999999994	0.07744499999999993		0.08049249999999994	0.08060749999999997	0.22466063949999981	0.22466063949999981	83	71.5	5.983182997000002	6.173816605000001	6.078499801000001	5.932128383500001	0.2070004437722748
B03	0.2		1	0.231275	0.30895	0.077675		0.0807225		0.2253371615	0.2253371615	86	71.5	5.783461937	5.788051995000001	5.785756966000001
D03		29	2	0.225875	0.54124	0.31536500000000006		0.3184125000000001		0.9244785275000003	1.8489570550000005	92	143.5	0.032848452999999986		0.032848452999999986	0.032848452999999986
E03		92	2	0.22999999999999998	0.23664999999999997	0.006649999999999989		0.009697499999999998		0.016424226499999993	0.032848452999999986
F03		29	10	0.226825	0.28954	0.06271500000000002		0.06576250000000003		0.18133381750000008	1.8133381750000008
G03		92	10	0.226575	0.22778	0.0012050000000000116		0.00425250000000002		4.083035000000592E-4	0.004083035000000592
A04	0.4		1	0.22885	0.36204000000000003	0.13319000000000003		0.13623750000000004	0.13886750000000003	0.3886289825000001	0.3886289825000001
B04	0.4		1	0.22665000000000002	0.36510000000000004	0.13845000000000002		0.14149750000000003		0.40410074650000005	0.40410074650000005
D04		32	2	0.22572499999999998	0.5568100000000001	0.3310850000000002		0.33413250000000017		0.9707173355000004	1.9414346710000008
F04		32	10	0.223625	0.28759	0.06396500000000002		0.06701250000000003		0.18501056750000008	1.850105675000001
A05	0.6		1	0.22599999999999998	0.4281499999999999	0.20214999999999994		0.20519749999999995	0.20680749999999998	0.5914679264999998	0.5914679264999998
B05	0.6		1	0.22444999999999998	0.42982	0.20537		0.2084175		0.6009392345	0.6009392345
D05		44	2	0.2239	0.8293799999999999	0.6054799999999999		0.6085274999999999		1.7778227884999995	3.555645576999999
F05		44	10	0.21975	0.33687	0.11712		0.12016750000000001		0.3413606845	3.4136068450000003
A06	0.8		1	0.2291	0.4999599999999999	0.2708599999999999		0.27390749999999986	0.2766949999999999	0.7935715204999996	0.7935715204999996
B06	0.8		1	0.22147499999999998	0.49790999999999996	0.276435		0.27948249999999997		0.8099698254999999	0.8099698254999999
D06		47	2	0.2214	0.76903	0.54763		0.5506774999999999		1.6076627984999996	3.215325596999999
F06		47	10	0.22107500000000002	0.33254	0.11146499999999998		0.11451249999999999		0.32472706749999997	3.2472706749999998
A07	1		1	0.22385	0.5397	0.31584999999999996		0.31889749999999994	0.3555674999999999	0.9259051064999998	0.9259051064999998
B07	1		1	0.22410000000000002	0.6132899999999999	0.38918999999999987		0.3922374999999999		1.1416273824999996	1.1416273824999996
D07		56	2	0.22230000000000003	1.044	0.8217		0.8247475		2.4138122964999997	4.8276245929999995
F07		56	10	0.220975	0.38552	0.16454499999999997		0.16759249999999998		0.4808565794999999	4.808565794999999
A08	1.2		1	0.230175	0.6272599999999999	0.3970849999999999		0.4001324999999999	0.40507	1.1648497354999996	1.1648497354999996
B08	1.2		1	0.22505	0.6320100000000001	0.4069600000000001		0.4100075000000001		1.1938960605000002	1.1938960605000002
D08		59	2	0.22612500000000002	1.0955499999999998	0.8694249999999998		0.8724724999999998		2.5541906114999993	5.1083812229999985
F08		59	10	0.221	0.38855999999999996	0.16755999999999996		0.17060749999999997		0.48972490049999984	4.897249004999998


	1	2	3	4	5	6	7	8	9	10	11	12
A	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
B	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
C
D	13	16	28	31	43	46	55	58	67	70	76	79	und
E	82	85	91
F	13	16	28	31	43	46	55	58	67	70	76	79	2x
G	82	85	91
H


	1	2	3	4	5	6	7	8	9	10	11	12
A	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
B	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
C
D	14	17	29	32	44	47	56	59	68	71	77	80	2x
E	83	86	92
F	14	17	29	32	44	47	56	59	68	71	77	80	10x
G	83	86	92
H


	1	2	3	4	5	6	7	8	9	10	11	12
A	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
B	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
C
D	15	18	30	33	45	48	57	60	69	72	78	81	5x
E	84	87	93
F	15	18	30	33	45	48	57	60	69	72	78	81	20x
G	84	87	93
H


	1	2	3	4	5	6	7	8	9	10	11	12
A	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
B	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
C
D	13	16	28	31	43	46	55	58	67	70	76	79	und
E	82	85	91
F	13	16	28	31	43	46	55	58	67	70	76	79	2x
G	82	85	91
H


	1	2	3	4	5	6	7	8	9	10	11	12
A	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
B	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
C
D	14	17	29	32	44	47	56	59	68	71	77	80	2x
E	83	86	92
F	14	17	29	32	44	47	56	59	68	71	77	80	10x
G	83	86	92
H


	1	2	3	4	5	6	7	8	9	10	11	12
A	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
B	0	0.1	0.2	0.4	0.6	0.8	1	1.2	2
C
D	15	18	30	33	45	48	57	60	69	72	78	81	5x
E	84	87	93
F	15	18	30	33	45	48	57	60	69	72	78	81	20x
G	84	87	93
H