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Fig 5D
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| 3 independent enzymatic reactions for each protein purification | |||||||||
| without ATP | with 1 mM ATP | ||||||||
| Kcat (sec-1) | wt NamPT 1mM | wt NamPT 100µM | Δ42-51 NamPT 1mM | Δ42-51 NamPT 100µM | wt NamPT 1mM | wt NamPT 100µM | Δ42-51 NamPT 1mM | Δ42-51 NamPT 100µM | |
| Purification 1 | 0.0112082122093023 | 0.0068316722037652265 | 0.009792378167641324 | ND | 0.009927273671096346 | 0.007578886351052047 | 0.00780743664717349 | ND | |
| 0.0116351917220377 | 0.006618182447397562 | 0.008866072124756338 | ND | 0.010354253183831671 | 0.00736539659468438 | 0.007013460038986352 | ND | ||
| 0.01142170196567 | 0.006831672203765226 | 0.009792378167641324 | ND | 0.010247508305647844 | 0.007365396594684391 | 0.007939766081871346 | ND | ||
| Purification 2 | 0.0122628035630567 | 0.006709835911861229 | 0.01036298117500275 | ND | 0.010527501172058132 | 0.006594149085794656 | 0.008635817645835625 | ND | |
| 0.0118000562587904 | 0.006941209563994372 | 0.009964404975964179 | ND | 0.010296127519924987 | 0.006709835911861226 | 0.008237241446797057 | ND | ||
| 0.011915743084857 | 0.006594149085794656 | ND | 0.010527501172058132 | 0.006709835911861228 | 0.008370100179809915 | ND | |||
| Purification 3 | 0.0108745616502579 | 0.006247088607594933 | 0.008000556074281562 | ND | 0.00913925925925926 | 0.004511786216596343 | 0.007324452744060586 | ND | |
| 0.0115686826066573 | 0.004627473042662914 | 0.008338607739392049 | ND | 0.007403956868260666 | 0.004627473042662914 | 0.007099084967320257 | ND | ||
| 0.010296127519925 | 0.004164725738396624 | ND | 0.007982390998593528 | 0.005321593999062352 | 0.006761033302209769 | ND | |||
| Purification 4 | 0.0112216221284576 | 0.007519643694327237 | x | x | 0.009833380215658697 | 0.007635330520393811 | x | x | |
| 0.0120314299109236 | 0.007288270042194093 | x | x | 0.01029612751992498 | 0.007403956868260662 | x | x | ||
| 0.0114529957805907 | 0.008098077824660101 | x | x | 0.010527501172058138 | x | x | |||
| Average | 0.011474094033377182 | 0.006539333363867848 | 0.009302482632097073 | 0.009755231754864367 | 0.006529421917901274 | 0.007687599228229377 | |||
| SD | 5.126369882418239E-4 | 0.0010685542640089499 | 8.330079164396447E-4 | 0.00100258354381768 | 0.001116134880525604 | 6.268256783964292E-4 | |||
| Data used for one-tailed Mann-Whitney test | |||||||||
| without ATP | with 1 mM ATP | ||||||||
| Average Kcat (sec-1) | wt NamPT 1mM | wt NamPT 100µM | Δ42-51 NamPT 1mM | Δ42-51 NamPT 100µM | wt NamPT 1mM | wt NamPT 100µM | Δ42-51 NamPT 1mM | Δ42-51 NamPT 100µM | |
| Purification 1 | 0.01142170196567 | 0.00676050895164267 | 0.00948360948667966 | ND | 0.010176345053525286 | 0.007436559846806939 | 0.00758688758934373 | ND | |
| Purification 2 | 0.011992867635568033 | 0.006748398187216753 | 0.010163693075483465 | ND | 0.010450376621347084 | 0.006671273636505703 | 0.008414386424147532 | ND | |
| Purification 3 | 0.0109131239256134 | 0.005013095796218157 | 0.008169581906836806 | ND | 0.00817520237537115 | 0.004820284419440536 | 0.007061523671196872 | ND | |
| Purification 4 | 0.0115686826066573 | 0.00763533052039381 | x | x | 0.010219002969213938 | 0.007519643694327236 | x | x | |
| n= | 4 | 4 | 3 | 3 | 4 | 4 | 3 | 3 | |
| ND | not detected | ||||||||
| wt vs Δ42-51 NamPT (1mM) wo ATP | p=0.0286 | significant | |||||||
| wt NamPT 100µM without vs with ATP | p=0.0571 | not significant | |||||||
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