Analysis of NAMPT WT and Δ42-51 by NMR

Selected Cell

Cell:

Value:

Fig 5D Sheet1 Sheet2 Sheet3

3 independent enzymatic reactions for each protein purification
	without ATP				with 1 mM ATP
Kcat (sec-1)	wt NamPT 1mM	wt NamPT 100µM	Δ42-51 NamPT 1mM	Δ42-51 NamPT 100µM	wt NamPT 1mM	wt NamPT 100µM	Δ42-51 NamPT 1mM	Δ42-51 NamPT 100µM
Purification 1	0.0112082122093023	0.0068316722037652265	0.009792378167641324	ND	0.009927273671096346	0.007578886351052047	0.00780743664717349	ND
	0.0116351917220377	0.006618182447397562	0.008866072124756338	ND	0.010354253183831671	0.00736539659468438	0.007013460038986352	ND
	0.01142170196567	0.006831672203765226	0.009792378167641324	ND	0.010247508305647844	0.007365396594684391	0.007939766081871346	ND
Purification 2	0.0122628035630567	0.006709835911861229	0.01036298117500275	ND	0.010527501172058132	0.006594149085794656	0.008635817645835625	ND
	0.0118000562587904	0.006941209563994372	0.009964404975964179	ND	0.010296127519924987	0.006709835911861226	0.008237241446797057	ND
	0.011915743084857	0.006594149085794656		ND	0.010527501172058132	0.006709835911861228	0.008370100179809915	ND
Purification 3	0.0108745616502579	0.006247088607594933	0.008000556074281562	ND	0.00913925925925926	0.004511786216596343	0.007324452744060586	ND
	0.0115686826066573	0.004627473042662914	0.008338607739392049	ND	0.007403956868260666	0.004627473042662914	0.007099084967320257	ND
	0.010296127519925	0.004164725738396624		ND	0.007982390998593528	0.005321593999062352	0.006761033302209769	ND
Purification 4	0.0112216221284576	0.007519643694327237	x	x	0.009833380215658697	0.007635330520393811	x	x
	0.0120314299109236	0.007288270042194093	x	x	0.01029612751992498	0.007403956868260662	x	x
	0.0114529957805907	0.008098077824660101	x	x	0.010527501172058138		x	x

Average	0.011474094033377182	0.006539333363867848	0.009302482632097073		0.009755231754864367	0.006529421917901274	0.007687599228229377
SD	5.126369882418239E-4	0.0010685542640089499	8.330079164396447E-4		0.00100258354381768	0.001116134880525604	6.268256783964292E-4

Data used for one-tailed Mann-Whitney test
	without ATP				with 1 mM ATP
Average Kcat (sec-1)	wt NamPT 1mM	wt NamPT 100µM	Δ42-51 NamPT 1mM	Δ42-51 NamPT 100µM	wt NamPT 1mM	wt NamPT 100µM	Δ42-51 NamPT 1mM	Δ42-51 NamPT 100µM
Purification 1	0.01142170196567	0.00676050895164267	0.00948360948667966	ND	0.010176345053525286	0.007436559846806939	0.00758688758934373	ND
Purification 2	0.011992867635568033	0.006748398187216753	0.010163693075483465	ND	0.010450376621347084	0.006671273636505703	0.008414386424147532	ND
Purification 3	0.0109131239256134	0.005013095796218157	0.008169581906836806	ND	0.00817520237537115	0.004820284419440536	0.007061523671196872	ND
Purification 4	0.0115686826066573	0.00763533052039381	x	x	0.010219002969213938	0.007519643694327236	x	x
n=	4	4	3	3	4	4	3	3

ND	not detected

wt vs Δ42-51 NamPT (1mM) wo ATP		p=0.0286	significant
wt NamPT 100µM without vs with ATP		p=0.0571	not significant