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Metadata
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Metadata | Values (examples) | ||||||||
Asset Title | One pot cascade analysis | ||||||||
Uploader | Jacky Snoep | ||||||||
Uploader SEEK ID | 49 | ||||||||
Project | Computational pathway design for biotechnology | ||||||||
ASSAY | |||||||||
Assay SEEK ID | 991 | ||||||||
Assay Title | One pot cascade analysis | ||||||||
Assay_type | enzymaticAssay | ||||||||
Technology_type | One pot cascade analysis | ||||||||
Description | One pot cascade analysis for the Weimberg pathway in Caulobacter crescentus, with NAD recycling, old enzymes with optimal protein distribution | ||||||||
Experimentalist | Lu Shen | ||||||||
Date | |||||||||
SOP | |||||||||
Publication (optional) | |||||||||
Experimental_conditions | |||||||||
Item | temperature | ||||||||
Compound (if concentration) | |||||||||
Unit | degrees celcius | ||||||||
Start_value (optional) | 37 | ||||||||
End_value (optional) | 37 | ||||||||
Comments | |||||||||
Culture growth | CultureGrowth | ||||||||
FACTORS_STUDIED | |||||||||
Item | concentration | concentration | |||||||
Compound (if concentration) | Xyl | NAD | |||||||
Unit | mM | mM | |||||||
Start_value (optional) | 5 | 10 | |||||||
End_value (optional) | |||||||||
SD (optional) | |||||||||
Comments | |||||||||
ENZYME IDENTITY | |||||||||
Enzyme Name (IUBMB) | Xylose dehydrogenase | D-Xylono-1,4-lactonase | D-Xylonate dehydratase | 2-keto-3-deoxy-D-xylonate dehydratase | α-ketoglutarate semialdehyde dehydrogenase | ||||
EC Number | 1.1.1.175 | 3.1.1.68 | 4.2.1.82 | ||||||
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time | NAD | LAC | XYL | XA | KG | NAD2 | LAC2 | XYL2 | XA2 | KG2 | NAD3 | LAC3 | XYL3 | XA3 | KG3 |
min | mM | mM | mM | mM | mM | mM | mM | mM | mM | mM | mM | mM | mM | mM | mM |
5 | 12.944102518723163 | 0.6562716644796387 | 2.9684710179065092 | 0.7489327735881361 | 0.32423430097716466 | 10.634748218396712 | 0.634491147286887 | 4.4106561181013255 | 0.5204382132708001 | 0.1617538046409531 | 10.942194738837166 | 0.6462674805863369 | 4.631018660228523 | 0.5803400352438619 | 0.11368514732886378 |
10 | 13.214628751907433 | 1.1256609685911914 | 2.5968323730955323 | 0.7911257612430771 | 0.46058600409545847 | 10.705662142722456 | 1.7903908631607321 | 3.0303548628955923 | 1.2599278442617214 | 0.3909312675478278 | 10.98424029901455 | 1.952351881857052 | 2.461265287366145 | 1.0592451085112478 | 0.31924666666592577 |
15 | 12.88050098114044 | 2.173147651230553 | 1.7593103063628313 | 1.099260981702744 | 0.8589646797711417 | 10.556152527095879 | 3.0332629054407745 | 1.326445787726037 | 2.3091291451895355 | 0.7050423590059296 | 10.950477299539273 | 3.3244545472218556 | 1.2519766294658097 | 1.8771018308433793 | 0.6230717576297397 |
20 | 12.517546484369204 | 3.2042484835889686 | 1.134665990953566 | 1.0693890287284744 | 0.970681787842203 | 10.533383615609878 | 4.026208876802074 | 0.6241382920267008 | 2.2252228865955566 | 0.8736149310503398 | 11.00699506886603 | 4.299830733421862 | 0.35507369596848165 | 2.543499866309485 | 0.811997504443738 |
25 | 12.408831005707553 | 4.114241796861624 | 0.7580564494295027 | 1.1283564671267783 | 1.4006913446125326 | 10.605096897832997 | 4.6736025099651215 | 0.19568511766820817 | 2.3386268942376027 | 1.1797745299649138 | 11.079503683433705 | 4.853602116522338 | 0.10266397955314074 | 2.3431401796542533 | 1.1336850618074712 |
30 | 12.345308912869207 | 4.950823534594966 | 0.45785951999074903 | 1.152370581149708 | 1.6919341146783617 | 10.559191638890802 | 5.095389504542042 | 0.12868857863514602 | 2.1622037270429257 | 1.4332698315590435 | 11.04325976984793 | 5.1651876364828695 | -0.1542320234313361 | 2.489844997773788 | 1.4493416529744807 |
35 | 12.452531333570601 | 5.684130972434087 | 0.03024089628767379 | 0.7850549220366003 | 1.8974051636832172 | 10.495916389577808 | 5.386050103493824 | 0.019815019437473737 | 1.9618555839235063 | 1.6462480512524817 | 11.327637038884168 | 5.563419450529881 | 0.10793258958007153 | 1.3216690282027124 | 1.5715613489065485 |
40 | 12.36612348823504 | 6.0769117464819775 | 0.025878061585391594 | 0.5949623709370222 | 1.93536951831485 | 10.532114052809526 | 5.690996210057801 | 0.01640695047643033 | 1.6049812515409236 | 1.9059974376945306 | 11.066786726856868 | 5.770312594346169 | 0.001343790214914178 | 2.105530447017242 | 1.919474250003332 |
45 | 12.385860485418982 | 6.442102565061592 | 0.02373721466304206 | 0.2822868060509496 | 2.409192563566897 | 10.802118936872802 | 5.943378298452435 | 0.20141678608571345 | 1.2514790468071242 | 2.20219940102088 | 11.307383492238674 | 6.139970774774868 | 0.015161006308789818 | 0.8104540333529241 | 2.138349854692445 |
50 | 12.516548217627676 | 6.734290699436274 | 0.04943625648567215 | 0.1275699511258735 | 2.560920711800896 | 10.89740375495162 | 6.1860953887891945 | 0.002504490827039205 | 1.0845863364399393 | 2.396725971198772 | 11.056178556609373 | 6.309154724078779 | 0.01848621108093784 | 1.4493500797348586 | 2.3706209899919046 |
55 | 12.511106466494846 | 6.976874068976951 | 0.026083672239627462 | 0.20116226150182026 | 2.632870044179442 | 10.803193645646852 | 6.418897799645995 | 0.11582379479052388 | 1.2456742009448205 | 2.6182097691475943 | 11.315105976796797 | 6.6556386744135665 | 0.07351209564166719 | 0.5810782250187365 | 2.5937565932666624 |
60 | 12.76696059512873 | 7.289251527593934 | 0.09122159198787555 | 0.20275850279042176 | 3.0166856551873145 | 10.713711723558957 | 6.6881206957019135 | -0.07499077251700453 | 0.9026784006906519 | 2.8586937893031834 | 11.33320317963577 | 6.865461534279287 | 0.03249488315636285 | 0.5585820312336022 | 2.8666098395100272 |
65 | 12.16413702937155 | 7.378426482655184 | -0.13000854798148512 | 0.06101210188690877 | 3.204255914721716 | 10.860759167448055 | 6.873867093226105 | 0.07225477254600336 | 0.6296458252322623 | 3.0404208001287314 | 11.244004387488872 | 7.108505865613067 | -0.08219942070226534 | 0.29185873686875874 | 3.0592172353055425 |
70 | 12.58733234550629 | 7.826524313808464 | 0.02752364195875066 | 0.2597161655026626 | 3.299668285037687 | 10.736826048600502 | 7.0822343491673925 | 0.047104868495929715 | 0.6947677835311334 | 3.265242943206495 | 11.192340683410995 | 7.2026741076969545 | -0.04926871517282225 | 0.4097692165059418 | 3.1858765144742813 |
75 | 12.34655865809887 | 8.086667621017062 | -0.10062279518316608 | 0.12090334943835186 | 3.5100442971988937 | 10.861215528865928 | 7.289491599856519 | 0.10962291795720695 | 0.16852320052675143 | 3.3843340053021373 | 11.372164901383393 | 7.532839472958125 | -0.038391366302628196 | 0.156354479747928 | 3.401203950359325 |
80 | 12.452660951128898 | 8.147111407972645 | -0.023107423409616704 | 0.2615038956090595 | 3.654518578401167 | 10.72645673362464 | 7.449892936909393 | 0.11260866295499092 | 0.1453804224754614 | 3.576585278498748 | 11.196528361051802 | 7.563061881719888 | -0.0783602491968172 | 0.1431157951105968 | 3.5784625100324474 |
85 | 12.589763469982376 | -0.03081067968494572 | 0.16720889509650744 | 11.005191013020308 | 7.727210997530294 | 0.06472745145117936 | 0.24105858287187842 | 3.7888416143138293 | 11.23493425035883 | 7.795467815411471 | 0.0067889322229106736 | 0.046641041979685674 | 3.6734583587641403 | ||
90 | 12.422499043202146 | 8.696585484409942 | -0.3889309583781855 | 0.08699575529379358 | 4.1206524159050675 | 10.814914386867203 | 7.671010770788799 | 0.020731939252334693 | 0.06768183413063505 | 3.708766351988075 | 11.438376479365857 | 7.884214400664488 | 0.04987697739426748 | -0.021463937153936713 | 3.6637038698087565 |
95 | 12.474999169507551 | 8.909286808288632 | 0.15127886693756776 | 0.12877478869565095 | 4.322450841029843 | 10.806404321841935 | 7.772220383627971 | -0.03972882622263783 | 0.017200244764624378 | 3.865687822393217 | 11.200334631749062 | 7.827407859800544 | -0.016861934291899177 | -0.003014537167506443 | 3.732371565085171 |
100 | 12.522590585029345 | 9.019742912159272 | -0.23055290300829143 | 0.12546476066681403 | 4.40446632114096 | 10.630790446304504 | 7.756073176016585 | -0.06886047313312679 | 0.03596525486933087 | 3.912158672378347 | 11.30316103698394 | 7.81854562066886 | 0.13853074456698028 | 0.018681012955601428 | 3.725656172098847 |
105 | 12.061103042807213 | 8.9561370192194 | -0.012641374636734938 | 0.04046284465592034 | 4.500849920626337 | 10.92120388636099 | 7.8347624947972 | 0.057832937257462906 | -0.03232138280976458 | 3.9062992402924217 | 11.203706283945849 | 7.905855234261123 | -0.062144609736924594 | 0.006266972548006276 | 3.815838346609785 |
110 | 12.670638509796216 | 9.252357963546144 | -0.08538941785880644 | 0.017164610318043783 | 4.599724396671805 | 10.708555496786119 | 7.7696182217718075 | 0.11914504207680822 | 0.011507600703359913 | 3.909748063539293 | 11.230887387575805 | 7.822932243238663 | -0.05297244787820678 | 0.03448985034209541 | 3.7360741523975145 |
115 | 12.549693883607338 | 9.289147995902292 | 0.007074839387868114 | 0.07096193445344456 | 4.6784404237206605 | 10.9572878130603 | 7.930836799590044 | 0.0871924458220282 | -0.03262359257099814 | 3.9369660088512957 | 11.358030103830878 | 7.903157102181785 | -0.0717741921508787 | 0.014823596925811259 | 3.80192864805738 |
120 | 12.255582491877302 | 9.14441933390731 | -0.08029623554120978 | 0.11700072799071452 | 4.518434093249102 | 10.82868161782368 | 7.865190674397957 | 0.04054207107970011 | 0.019741134509028868 | 3.8991838908601117 | 11.223768812160193 | 7.826838923854874 | 0.03569350347438572 | -0.02352918566476281 | 3.747920877635033 |
125 | 12.338652085256372 | 9.338981147798322 | -0.15856441870340315 | 0.43779161415142687 | 4.69314423558487 | 10.797156248004825 | 7.881223645474217 | 0.20336140951746556 | -0.06215689460836343 | 4.023856041313892 | 11.223768812160193 | 7.826838923854874 | 0.03569350347438572 | -0.02352918566476281 | 3.747920877635033 |
130 | 12.753725538020602 | 9.522905812828906 | 0.07379957646878618 | 0.12263758507935833 | 4.9371717226663705 | 10.756104417826638 | 7.797414576434751 | 0.0015747981678098971 | -0.014038893029165946 | 3.948035619954809 | 11.223768812160193 | 7.826838923854874 | 0.03569350347438572 | -0.02352918566476281 | 3.747920877635033 |
135 | 12.272338008743645 | 9.28357740708533 | -0.07808716677602331 | 0.11901725637818542 | 4.571268284869829 | 10.809711083706077 | 7.850832069991743 | 0.19161022565696437 | -0.021155683454017922 | 3.9372153796276255 | 11.183714386781826 | 7.827258082990612 | -0.007049712781494005 | 0.009046373369284887 | 3.808252570462164 |
140 | 12.649462061558467 | 9.419784247654428 | -0.15523725031619198 | 0.0990097546278241 | 4.766054766001011 | 10.715684038840097 | 7.750167578907368 | -0.17838483483466128 | 0.02103713176200497 | 3.930961241242084 | 11.183714386781826 | 7.827258082990612 | -0.007049712781494005 | 0.009046373369284887 | 3.808252570462164 |
145 | 12.595497928635416 | 0.07208790827810083 | 0.11556687011265027 | 10.739711340823593 | 7.8090311421173855 | 0.049026292330864064 | 0.04405907756297053 | 3.9301707069077603 | 11.14753316153297 | 7.807971468139687 | -0.08658770575971633 | -0.02827359342868646 | 3.795871996160096 | ||
150 | 12.692850207117754 | 9.499473409559776 | 0.17871685777673318 | 0.1288287549679043 | 4.789906165200665 | 10.697528095474553 | 7.8187844870083225 | 0.10297275618429202 | -0.00964122982056895 | 3.990334025134371 | 11.14753316153297 | 7.807971468139687 | -0.08658770575971633 | -0.02827359342868646 | 3.795871996160096 |
155 | 12.18041148923133 | 9.261175722674702 | 0.009729295756754224 | 0.0835021014783957 | 4.725292542503711 | 10.755149784105559 | 7.767925813161471 | 0.16788695047939142 | 0.03709663109362302 | 3.8914807160061917 | 11.216487487015872 | 7.845806168988395 | 0.05578539593071332 | -0.0045100185695112404 | 3.8201105114984975 |
160 | 12.277015185126377 | 9.26987872195456 | 0.020084928494023595 | 0.03791979283373389 | 4.674512914810905 | 10.669008555137642 | 7.787856506518047 | -0.003323636832395965 | -0.007635065419746505 | 3.887583264077122 | 11.216487487015872 | 7.845806168988395 | 0.05578539593071332 | -0.0045100185695112404 | 3.8201105114984975 |
165 | 12.375338361534167 | 9.37493875316303 | 0.04565335239778613 | 0.1697549952184577 | 4.765109128996441 | 10.727119280940647 | 7.7456884351865245 | 0.04445458698246047 | 0.03146523235354665 | 3.902632007224056 | 11.082414653051 | 7.7699585985942825 | -0.15943875025513646 | -0.015896184767986207 | 3.8355988195591633 |
170 | 12.451437894910264 | 9.367806247608804 | -0.030746844417780972 | 0.2541337284121508 | 4.707485202901784 | 10.707151028819892 | 7.772108519893274 | 0.08026573269654684 | 0.005598170011344612 | 3.9816502611292375 | 11.110644586124936 | 7.7719017572412525 | -0.039126815874219935 | -0.012461655152228964 | 3.788254440666737 |
175 | 12.341734891064661 | 9.350761163064163 | 0.022506318756813096 | 0.06753769501160686 | 4.804851633793439 | 10.685299556908934 | 7.741891109906338 | -0.12224769908794687 | -0.040605177567005296 | 3.904970167313268 | 11.024193521677873 | 7.82040363764121 | 0.05354197316843104 | -0.004179901594152797 | 3.816078848019121 |
180 | 12.454060095538459 | 9.350745911949309 | 0.026950124586841536 | 0.13125900524766965 | 4.6972461351819605 | 10.654282124300092 | 7.734510227295626 | 0.06909063667701865 | -0.06920314548581911 | 3.850482720919305 | 11.043321748187033 | 7.754004351464536 | -0.04532988192544245 | 0.005261860743327792 | 3.803431366891105 |
185 | 12.55674573827025 | 9.405339784964195 | -0.1024815501168787 | 0.12028438003489558 | 4.7781451193084035 | 10.732707443093545 | 7.75960336857216 | 0.21483623114749006 | 0.0039089212025078465 | 3.856165351800866 | 10.963965963231441 | 7.732109721563894 | -0.0810804831228705 | 0.013814656697474254 | 3.834276832710899 |
190 | 12.225161048033852 | 9.349176583836375 | 0.030220215616617314 | 0.031253085985334585 | 4.7154999560607065 | 10.553017846408263 | 7.763067164884053 | 0.04207473504687009 | -0.03992180216115208 | 3.9535655517775274 | 11.140614517783744 | 7.813940392464405 | 0.07891811029757223 | 0.022848444863969534 | 3.818431989860988 |
195 | 12.358753787086165 | 9.373867311789539 | -0.08713099226604198 | 0.040726042677512744 | 4.754184965163963 | 10.637362611930769 | 7.683978066027082 | 0.38982344688824433 | 0.03326806524648672 | 3.898348992708074 | 10.974995852192574 | 7.845978363660855 | -0.11349021944059817 | 0.012080010475507648 | 3.9668991529224993 |
200 | 12.482232729560327 | 9.373347311340751 | 0.05219651287564987 | 0.07982865083851391 | 4.692447865909182 | 10.686387855649356 | 7.704851705629876 | -0.2201400059233789 | -0.0446686983130777 | 3.874797680548866 | 10.936095611343038 | 7.829326245465563 | 0.058960896406262335 | 0.01726789642127613 | 3.8754035482551914 |
205 | 12.429905870938502 | 9.389534790943694 | -0.06252077873119163 | 0.1313450413091259 | 4.697340572093626 | 10.66607208907763 | 7.714011440610722 | 0.11442396472224732 | 0.028143054529395483 | 3.8213731854047897 | 10.994549197718237 | 7.747143126684003 | 0.013581637256546264 | -0.017621451912855128 | 3.7374382859072193 |
210 | 12.349115679972584 | 9.38620198740431 | 0.16064236909369584 | -0.08033123153383066 | 4.84432049928663 | 10.71494124355802 | 7.686845381127494 | 0.21585378389048296 | 0.0203929554950274 | 3.8321071225658656 | 10.99507496943916 | 7.787741129777198 | 0.0770959235242969 | 0.07825500673833555 | 3.809634240646123 |
215 | 12.324763136141081 | 9.356235030416077 | -0.03205084604742877 | -0.006636957120752878 | 4.728941239746498 | 10.616342180896261 | 7.66472613550339 | 0.3457431066117958 | 0.06026609064055832 | 3.8108327272992892 | 11.090736436332357 | 7.781582553447613 | -0.03409288999570278 | 0.023836212104037836 | 3.7718677421416302 |
220 | 12.34007033120011 | 9.424020964671326 | 0.009685499517659889 | 0.037770964657458786 | 4.672201771361735 | 10.733497972456687 | 7.720247347020443 | -0.0815903870728375 | -0.037080517575065336 | 3.9261871316382466 | 11.090736436332357 | 7.781582553447613 | -0.03409288999570278 | 0.023836212104037836 | 3.7718677421416302 |
225 | 12.727842401338169 | -0.010179574720177609 | 0.10391971247900766 | 10.738064996929975 | 7.740096711365469 | 0.024314427048843578 | -0.01967674483549352 | 3.7943468195042955 | 11.16001631535962 | 7.791972210383601 | -0.07373837776379119 | 8.426754807035015E-4 | 3.8699203122478787 | ||
230 | 12.456018149160183 | 9.41330966190697 | 0.016308062512727897 | 0.10219085690823423 | 4.7154090632537 | 10.601192756941078 | 7.635569597978859 | -0.07599196364753905 | 0.014109344588286029 | 3.8389700097528463 | 11.187542707253526 | 7.812732327166721 | 0.06497892043908036 | 0.006796894860225541 | 3.8245548297085374 |
235 | 12.612567607990934 | 9.375738684360348 | 9.419135749420243E-4 | 0.13300553379281865 | 4.7640852375349825 | 10.681706636592308 | 7.674212547401592 | 0.2441694178310217 | -0.02617478378992094 | 3.886638598284817 | 11.01521661284773 | 7.7179493357699025 | -0.06718057279096754 | -0.014330502445702664 | 3.8036931637005154 |
240 | 12.665350540409488 | 9.452998315979483 | 0.11790388749606076 | 0.23250881684912114 | 4.744414482870093 | 10.648663175743147 | 7.665666455653143 | 0.04864015251165578 | 0.05531758111430121 | 3.8985382213945585 | 10.959058757774852 | 7.814706209969047 | 0.060590268146943495 | -0.01765690907969969 | 3.8516670423989052 |
245 | 10.67087011735018 | 7.647995651194119 | 0.0048036061007080706 | 0.0049458471576831016 | 3.7480922491101443 | 10.959058757774852 | 7.814706209969047 | 0.060590268146943495 | -0.01765690907969969 | 3.8516670423989052 | |||||
250 | 10.728317363046376 | 7.731758807263887 | 0.2628593776475574 | 0.07960476801909534 | 3.8392822061128875 | 11.165915676954157 | 7.805639169747895 | -0.15702687042779323 | 0.007172891546869576 | 3.824257403405109 | |||||
255 | 10.646805663543548 | 7.675437798863165 | 0.19827359599268524 | -0.0015945414539605112 | 3.8010027396142614 | 11.043153610278939 | 7.7253216657547945 | 0.009511174347290617 | 0.019621840895865005 | 3.791211738310932 | |||||
260 | 10.542509958802961 | 7.643651255738595 | 0.25073395971814955 | 0.07612080547381403 | 3.8376742961394035 | 11.083541148537648 | 7.742410825159817 | -0.0633641771003894 | -9.479974550892709E-4 | 3.8412394442434286 | |||||
265 | 10.569648175429663 | 7.601380898979105 | -0.2777156276433973 | 0.03970718812945637 | 3.86864710177539 | 11.134147862003566 | 7.803485727533137 | -0.15475333409066686 | 0.02707629133544473 | 3.8272076896203555 | |||||
270 | 10.736650524631962 | 7.667242893025566 | -0.18355067379610998 | -0.20072493747805176 | 3.792025395463288 | 11.11678957602749 | 7.778048878683464 | -0.0474500309151668 | -0.015340532045746716 | 3.789082492727709 | |||||
275 | 10.700459607540227 | 7.69534170715187 | 0.17812738949998597 | 0.02228442356474602 | 3.8268975659136895 | 11.074792064280876 | 7.77409515588984 | 0.07610983815199526 | 0.012785363824594064 | 3.783495773019874 | |||||
280 | 10.62112482443885 | 7.656242599649293 | 0.04336228785793006 | 0.0760654225516828 | 3.8675088514089753 | 11.006937804885222 | 7.772603697317924 | -0.12793734878720853 | -0.0189104624653826 | 3.8376767384481134 | |||||
285 | 10.592191616853594 | 7.613286626950724 | 0.036559868437031566 | 0.04114464645145328 | 3.793591535172463 | 10.96738622738075 | 7.764168527626137 | 0.047527322494908306 | 0.027275307795717886 | 3.824210988270692 | |||||
290 | 10.549189266840546 | 7.564999928625664 | -0.2388141319907014 | 0.10390064148534751 | 3.7844485969957975 | 10.998881673293672 | 7.748680728313268 | -0.013992254504514127 | 0.028152551581285858 | 3.7784519748168277 | |||||
295 | 10.590276453374743 | 7.640702648959143 | -0.03437540978820521 | -0.13778662184867826 | 3.8099825533660567 | 11.068049982195202 | 7.7750762108776446 | -0.2086692836983695 | 0.002571820024728194 | 3.84442881509923 | |||||
300 | 10.639060303175292 | 7.663814680269833 | 0.05435721986620577 | 0.11535193698818291 | 3.895212323141436 | 10.920678803994262 | 7.72186619852175 | -0.011942909657440371 | 0.038121226304865474 | 3.785648278543081 |
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time (min) | NAD (mM) | LAC (mM) | XYL (mM) | XA (mM) | KG (mM) | ||||
5 | 12.944102518723163 | 0.6562716644796387 | 2.9684710179065092 | 0.7489327735881361 | 0.32423430097716466 | ||||
10 | 13.214628751907433 | 1.1256609685911914 | 2.5968323730955323 | 0.7911257612430771 | 0.46058600409545847 | ||||
15 | 12.88050098114044 | 2.173147651230553 | 1.7593103063628313 | 1.099260981702744 | 0.8589646797711417 | ||||
20 | 12.517546484369204 | 3.2042484835889686 | 1.134665990953566 | 1.0693890287284744 | 0.970681787842203 | ||||
25 | 12.408831005707553 | 4.114241796861624 | 0.7580564494295027 | 1.1283564671267783 | 1.4006913446125326 | ||||
30 | 12.345308912869207 | 4.950823534594966 | 0.45785951999074903 | 1.152370581149708 | 1.6919341146783617 | ||||
35 | 12.452531333570601 | 5.684130972434087 | 0.03024089628767379 | 0.7850549220366003 | 1.8974051636832172 | ||||
40 | 12.36612348823504 | 6.0769117464819775 | 0.025878061585391594 | 0.5949623709370222 | 1.93536951831485 | ||||
45 | 12.385860485418982 | 6.442102565061592 | 0.02373721466304206 | 0.2822868060509496 | 2.409192563566897 | ||||
50 | 12.516548217627676 | 6.734290699436274 | 0.04943625648567215 | 0.1275699511258735 | 2.560920711800896 | ||||
55 | 12.511106466494846 | 6.976874068976951 | 0.026083672239627462 | 0.20116226150182026 | 2.632870044179442 | ||||
60 | 12.76696059512873 | 7.289251527593934 | 0.09122159198787555 | 0.20275850279042176 | 3.0166856551873145 | ||||
65 | 12.16413702937155 | 7.378426482655184 | -0.13000854798148512 | 0.06101210188690877 | 3.204255914721716 | ||||
70 | 12.58733234550629 | 7.826524313808464 | 0.02752364195875066 | 0.2597161655026626 | 3.299668285037687 | ||||
75 | 12.34655865809887 | 8.086667621017062 | -0.10062279518316608 | 0.12090334943835186 | 3.5100442971988937 | ||||
80 | 12.452660951128898 | 8.147111407972645 | -0.023107423409616704 | 0.2615038956090595 | 3.654518578401167 | ||||
85 | 12.589763469982376 | -0.03081067968494572 | 0.16720889509650744 | ||||||
90 | 12.422499043202146 | 8.696585484409942 | -0.3889309583781855 | 0.08699575529379358 | 4.1206524159050675 | ||||
95 | 12.474999169507551 | 8.909286808288632 | 0.15127886693756776 | 0.12877478869565095 | 4.322450841029843 | ||||
100 | 12.522590585029345 | 9.019742912159272 | -0.23055290300829143 | 0.12546476066681403 | 4.40446632114096 | ||||
105 | 12.061103042807213 | 8.9561370192194 | -0.012641374636734938 | 0.04046284465592034 | 4.500849920626337 | ||||
110 | 12.670638509796216 | 9.252357963546144 | -0.08538941785880644 | 0.017164610318043783 | 4.599724396671805 | ||||
115 | 12.549693883607338 | 9.289147995902292 | 0.007074839387868114 | 0.07096193445344456 | 4.6784404237206605 | ||||
120 | 12.255582491877302 | 9.14441933390731 | -0.08029623554120978 | 0.11700072799071452 | 4.518434093249102 | ||||
125 | 12.338652085256372 | 9.338981147798322 | -0.15856441870340315 | 0.43779161415142687 | 4.69314423558487 | ||||
130 | 12.753725538020602 | 9.522905812828906 | 0.07379957646878618 | 0.12263758507935833 | 4.9371717226663705 | ||||
135 | 12.272338008743645 | 9.28357740708533 | -0.07808716677602331 | 0.11901725637818542 | 4.571268284869829 | ||||
140 | 12.649462061558467 | 9.419784247654428 | -0.15523725031619198 | 0.0990097546278241 | 4.766054766001011 | ||||
145 | 12.595497928635416 | 0.07208790827810083 | 0.11556687011265027 | ||||||
150 | 12.692850207117754 | 9.499473409559776 | 0.17871685777673318 | 0.1288287549679043 | 4.789906165200665 | ||||
155 | 12.18041148923133 | 9.261175722674702 | 0.009729295756754224 | 0.0835021014783957 | 4.725292542503711 | ||||
160 | 12.277015185126377 | 9.26987872195456 | 0.020084928494023595 | 0.03791979283373389 | 4.674512914810905 | ||||
165 | 12.375338361534167 | 9.37493875316303 | 0.04565335239778613 | 0.1697549952184577 | 4.765109128996441 | ||||
170 | 12.451437894910264 | 9.367806247608804 | -0.030746844417780972 | 0.2541337284121508 | 4.707485202901784 | ||||
175 | 12.341734891064661 | 9.350761163064163 | 0.022506318756813096 | 0.06753769501160686 | 4.804851633793439 | ||||
180 | 12.454060095538459 | 9.350745911949309 | 0.026950124586841536 | 0.13125900524766965 | 4.6972461351819605 | ||||
185 | 12.55674573827025 | 9.405339784964195 | -0.1024815501168787 | 0.12028438003489558 | 4.7781451193084035 | ||||
190 | 12.225161048033852 | 9.349176583836375 | 0.030220215616617314 | 0.031253085985334585 | 4.7154999560607065 | ||||
195 | 12.358753787086165 | 9.373867311789539 | -0.08713099226604198 | 0.040726042677512744 | 4.754184965163963 | ||||
200 | 12.482232729560327 | 9.373347311340751 | 0.05219651287564987 | 0.07982865083851391 | 4.692447865909182 | ||||
205 | 12.429905870938502 | 9.389534790943694 | -0.06252077873119163 | 0.1313450413091259 | 4.697340572093626 | ||||
210 | 12.349115679972584 | 9.38620198740431 | 0.16064236909369584 | -0.08033123153383066 | 4.84432049928663 | ||||
215 | 12.324763136141081 | 9.356235030416077 | -0.03205084604742877 | -0.006636957120752878 | 4.728941239746498 | ||||
220 | 12.34007033120011 | 9.424020964671326 | 0.009685499517659889 | 0.037770964657458786 | 4.672201771361735 | ||||
225 | 12.727842401338169 | -0.010179574720177609 | 0.10391971247900766 | ||||||
230 | 12.456018149160183 | 9.41330966190697 | 0.016308062512727897 | 0.10219085690823423 | 4.7154090632537 | ||||
235 | 12.612567607990934 | 9.375738684360348 | 9.419135749420243E-4 | 0.13300553379281865 | 4.7640852375349825 | ||||
240 | 12.665350540409488 | 9.452998315979483 | 0.11790388749606076 | 0.23250881684912114 | 4.744414482870093 |
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time (min) | NAD (mM) | LAC (mM) | XYL (mM) | XA (mM) | KG (mM) | ||||
5 | 10.634748218396712 | 0.634491147286887 | 4.4106561181013255 | 0.5204382132708001 | 0.1617538046409531 | ||||
10 | 10.705662142722456 | 1.7903908631607321 | 3.0303548628955923 | 1.2599278442617214 | 0.3909312675478278 | ||||
15 | 10.556152527095879 | 3.0332629054407745 | 1.326445787726037 | 2.3091291451895355 | 0.7050423590059296 | ||||
20 | 10.533383615609878 | 4.026208876802074 | 0.6241382920267008 | 2.2252228865955566 | 0.8736149310503398 | ||||
25 | 10.605096897832997 | 4.6736025099651215 | 0.19568511766820817 | 2.3386268942376027 | 1.1797745299649138 | ||||
30 | 10.559191638890802 | 5.095389504542042 | 0.12868857863514602 | 2.1622037270429257 | 1.4332698315590435 | ||||
35 | 10.495916389577808 | 5.386050103493824 | 0.019815019437473737 | 1.9618555839235063 | 1.6462480512524817 | ||||
40 | 10.532114052809526 | 5.690996210057801 | 0.01640695047643033 | 1.6049812515409236 | 1.9059974376945306 | ||||
45 | 10.802118936872802 | 5.943378298452435 | 0.20141678608571345 | 1.2514790468071242 | 2.20219940102088 | ||||
50 | 10.89740375495162 | 6.1860953887891945 | 0.002504490827039205 | 1.0845863364399393 | 2.396725971198772 | ||||
55 | 10.803193645646852 | 6.418897799645995 | 0.11582379479052388 | 1.2456742009448205 | 2.6182097691475943 | ||||
60 | 10.713711723558957 | 6.6881206957019135 | -0.07499077251700453 | 0.9026784006906519 | 2.8586937893031834 | ||||
65 | 10.860759167448055 | 6.873867093226105 | 0.07225477254600336 | 0.6296458252322623 | 3.0404208001287314 | ||||
70 | 10.736826048600502 | 7.0822343491673925 | 0.047104868495929715 | 0.6947677835311334 | 3.265242943206495 | ||||
75 | 10.861215528865928 | 7.289491599856519 | 0.10962291795720695 | 0.16852320052675143 | 3.3843340053021373 | ||||
80 | 10.72645673362464 | 7.449892936909393 | 0.11260866295499092 | 0.1453804224754614 | 3.576585278498748 | ||||
85 | 11.005191013020308 | 7.727210997530294 | 0.06472745145117936 | 0.24105858287187842 | 3.7888416143138293 | ||||
90 | 10.814914386867203 | 7.671010770788799 | 0.020731939252334693 | 0.06768183413063505 | 3.708766351988075 | ||||
95 | 10.806404321841935 | 7.772220383627971 | -0.03972882622263783 | 0.017200244764624378 | 3.865687822393217 | ||||
100 | 10.630790446304504 | 7.756073176016585 | -0.06886047313312679 | 0.03596525486933087 | 3.912158672378347 | ||||
105 | 10.92120388636099 | 7.8347624947972 | 0.057832937257462906 | -0.03232138280976458 | 3.9062992402924217 | ||||
110 | 10.708555496786119 | 7.7696182217718075 | 0.11914504207680822 | 0.011507600703359913 | 3.909748063539293 | ||||
115 | 10.9572878130603 | 7.930836799590044 | 0.0871924458220282 | -0.03262359257099814 | 3.9369660088512957 | ||||
120 | 10.82868161782368 | 7.865190674397957 | 0.04054207107970011 | 0.019741134509028868 | 3.8991838908601117 | ||||
125 | 10.797156248004825 | 7.881223645474217 | 0.20336140951746556 | -0.06215689460836343 | 4.023856041313892 | ||||
130 | 10.756104417826638 | 7.797414576434751 | 0.0015747981678098971 | -0.014038893029165946 | 3.948035619954809 | ||||
135 | 10.809711083706077 | 7.850832069991743 | 0.19161022565696437 | -0.021155683454017922 | 3.9372153796276255 | ||||
140 | 10.715684038840097 | 7.750167578907368 | -0.17838483483466128 | 0.02103713176200497 | 3.930961241242084 | ||||
145 | 10.739711340823593 | 7.8090311421173855 | 0.049026292330864064 | 0.04405907756297053 | 3.9301707069077603 | ||||
150 | 10.697528095474553 | 7.8187844870083225 | 0.10297275618429202 | -0.00964122982056895 | 3.990334025134371 | ||||
155 | 10.755149784105559 | 7.767925813161471 | 0.16788695047939142 | 0.03709663109362302 | 3.8914807160061917 | ||||
160 | 10.669008555137642 | 7.787856506518047 | -0.003323636832395965 | -0.007635065419746505 | 3.887583264077122 | ||||
165 | 10.727119280940647 | 7.7456884351865245 | 0.04445458698246047 | 0.03146523235354665 | 3.902632007224056 | ||||
170 | 10.707151028819892 | 7.772108519893274 | 0.08026573269654684 | 0.005598170011344612 | 3.9816502611292375 | ||||
175 | 10.685299556908934 | 7.741891109906338 | -0.12224769908794687 | -0.040605177567005296 | 3.904970167313268 | ||||
180 | 10.654282124300092 | 7.734510227295626 | 0.06909063667701865 | -0.06920314548581911 | 3.850482720919305 | ||||
185 | 10.732707443093545 | 7.75960336857216 | 0.21483623114749006 | 0.0039089212025078465 | 3.856165351800866 | ||||
190 | 10.553017846408263 | 7.763067164884053 | 0.04207473504687009 | -0.03992180216115208 | 3.9535655517775274 | ||||
195 | 10.637362611930769 | 7.683978066027082 | 0.38982344688824433 | 0.03326806524648672 | 3.898348992708074 | ||||
200 | 10.686387855649356 | 7.704851705629876 | -0.2201400059233789 | -0.0446686983130777 | 3.874797680548866 | ||||
205 | 10.66607208907763 | 7.714011440610722 | 0.11442396472224732 | 0.028143054529395483 | 3.8213731854047897 | ||||
210 | 10.71494124355802 | 7.686845381127494 | 0.21585378389048296 | 0.0203929554950274 | 3.8321071225658656 | ||||
215 | 10.616342180896261 | 7.66472613550339 | 0.3457431066117958 | 0.06026609064055832 | 3.8108327272992892 | ||||
220 | 10.733497972456687 | 7.720247347020443 | -0.0815903870728375 | -0.037080517575065336 | 3.9261871316382466 | ||||
225 | 10.738064996929975 | 7.740096711365469 | 0.024314427048843578 | -0.01967674483549352 | 3.7943468195042955 | ||||
230 | 10.601192756941078 | 7.635569597978859 | -0.07599196364753905 | 0.014109344588286029 | 3.8389700097528463 | ||||
235 | 10.681706636592308 | 7.674212547401592 | 0.2441694178310217 | -0.02617478378992094 | 3.886638598284817 | ||||
240 | 10.648663175743147 | 7.665666455653143 | 0.04864015251165578 | 0.05531758111430121 | 3.8985382213945585 | ||||
245 | 10.67087011735018 | 7.647995651194119 | 0.0048036061007080706 | 0.0049458471576831016 | 3.7480922491101443 | ||||
250 | 10.728317363046376 | 7.731758807263887 | 0.2628593776475574 | 0.07960476801909534 | 3.8392822061128875 | ||||
255 | 10.646805663543548 | 7.675437798863165 | 0.19827359599268524 | -0.0015945414539605112 | 3.8010027396142614 | ||||
260 | 10.542509958802961 | 7.643651255738595 | 0.25073395971814955 | 0.07612080547381403 | 3.8376742961394035 | ||||
265 | 10.569648175429663 | 7.601380898979105 | -0.2777156276433973 | 0.03970718812945637 | 3.86864710177539 | ||||
270 | 10.736650524631962 | 7.667242893025566 | -0.18355067379610998 | -0.20072493747805176 | 3.792025395463288 | ||||
275 | 10.700459607540227 | 7.69534170715187 | 0.17812738949998597 | 0.02228442356474602 | 3.8268975659136895 | ||||
280 | 10.62112482443885 | 7.656242599649293 | 0.04336228785793006 | 0.0760654225516828 | 3.8675088514089753 | ||||
285 | 10.592191616853594 | 7.613286626950724 | 0.036559868437031566 | 0.04114464645145328 | 3.793591535172463 | ||||
290 | 10.549189266840546 | 7.564999928625664 | -0.2388141319907014 | 0.10390064148534751 | 3.7844485969957975 | ||||
295 | 10.590276453374743 | 7.640702648959143 | -0.03437540978820521 | -0.13778662184867826 | 3.8099825533660567 | ||||
300 | 10.639060303175292 | 7.663814680269833 | 0.05435721986620577 | 0.11535193698818291 | 3.895212323141436 |
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25
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29
30
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32
33
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35
36
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38
39
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time (min) | NAD (mM) | LAC (mM) | XYL (mM) | XA (mM) | KG (mM) | ||||
5 | 10.942194738837166 | 0.6462674805863369 | 4.631018660228523 | 0.5803400352438619 | 0.11368514732886378 | ||||
10 | 10.98424029901455 | 1.952351881857052 | 2.461265287366145 | 1.0592451085112478 | 0.31924666666592577 | ||||
15 | 10.950477299539273 | 3.3244545472218556 | 1.2519766294658097 | 1.8771018308433793 | 0.6230717576297397 | ||||
20 | 11.00699506886603 | 4.299830733421862 | 0.35507369596848165 | 2.543499866309485 | 0.811997504443738 | ||||
25 | 11.079503683433705 | 4.853602116522338 | 0.10266397955314074 | 2.3431401796542533 | 1.1336850618074712 | ||||
30 | 11.04325976984793 | 5.1651876364828695 | -0.1542320234313361 | 2.489844997773788 | 1.4493416529744807 | ||||
35 | 11.327637038884168 | 5.563419450529881 | 0.10793258958007153 | 1.3216690282027124 | 1.5715613489065485 | ||||
40 | 11.066786726856868 | 5.770312594346169 | 0.001343790214914178 | 2.105530447017242 | 1.919474250003332 | ||||
45 | 11.307383492238674 | 6.139970774774868 | 0.015161006308789818 | 0.8104540333529241 | 2.138349854692445 | ||||
50 | 11.056178556609373 | 6.309154724078779 | 0.01848621108093784 | 1.4493500797348586 | 2.3706209899919046 | ||||
55 | 11.315105976796797 | 6.6556386744135665 | 0.07351209564166719 | 0.5810782250187365 | 2.5937565932666624 | ||||
60 | 11.33320317963577 | 6.865461534279287 | 0.03249488315636285 | 0.5585820312336022 | 2.8666098395100272 | ||||
65 | 11.244004387488872 | 7.108505865613067 | -0.08219942070226534 | 0.29185873686875874 | 3.0592172353055425 | ||||
70 | 11.192340683410995 | 7.2026741076969545 | -0.04926871517282225 | 0.4097692165059418 | 3.1858765144742813 | ||||
75 | 11.372164901383393 | 7.532839472958125 | -0.038391366302628196 | 0.156354479747928 | 3.401203950359325 | ||||
80 | 11.196528361051802 | 7.563061881719888 | -0.0783602491968172 | 0.1431157951105968 | 3.5784625100324474 | ||||
85 | 11.23493425035883 | 7.795467815411471 | 0.0067889322229106736 | 0.046641041979685674 | 3.6734583587641403 | ||||
90 | 11.438376479365857 | 7.884214400664488 | 0.04987697739426748 | -0.021463937153936713 | 3.6637038698087565 | ||||
95 | 11.200334631749062 | 7.827407859800544 | -0.016861934291899177 | -0.003014537167506443 | 3.732371565085171 | ||||
100 | 11.30316103698394 | 7.81854562066886 | 0.13853074456698028 | 0.018681012955601428 | 3.725656172098847 | ||||
105 | 11.203706283945849 | 7.905855234261123 | -0.062144609736924594 | 0.006266972548006276 | 3.815838346609785 | ||||
110 | 11.230887387575805 | 7.822932243238663 | -0.05297244787820678 | 0.03448985034209541 | 3.7360741523975145 | ||||
115 | 11.358030103830878 | 7.903157102181785 | -0.0717741921508787 | 0.014823596925811259 | 3.80192864805738 | ||||
120 | 11.223768812160193 | 7.826838923854874 | 0.03569350347438572 | -0.02352918566476281 | 3.747920877635033 | ||||
125 | 11.223768812160193 | 7.826838923854874 | 0.03569350347438572 | -0.02352918566476281 | 3.747920877635033 | ||||
130 | 11.223768812160193 | 7.826838923854874 | 0.03569350347438572 | -0.02352918566476281 | 3.747920877635033 | ||||
135 | 11.183714386781826 | 7.827258082990612 | -0.007049712781494005 | 0.009046373369284887 | 3.808252570462164 | ||||
140 | 11.183714386781826 | 7.827258082990612 | -0.007049712781494005 | 0.009046373369284887 | 3.808252570462164 | ||||
145 | 11.14753316153297 | 7.807971468139687 | -0.08658770575971633 | -0.02827359342868646 | 3.795871996160096 | ||||
150 | 11.14753316153297 | 7.807971468139687 | -0.08658770575971633 | -0.02827359342868646 | 3.795871996160096 | ||||
155 | 11.216487487015872 | 7.845806168988395 | 0.05578539593071332 | -0.0045100185695112404 | 3.8201105114984975 | ||||
160 | 11.216487487015872 | 7.845806168988395 | 0.05578539593071332 | -0.0045100185695112404 | 3.8201105114984975 | ||||
165 | 11.082414653051 | 7.7699585985942825 | -0.15943875025513646 | -0.015896184767986207 | 3.8355988195591633 | ||||
170 | 11.110644586124936 | 7.7719017572412525 | -0.039126815874219935 | -0.012461655152228964 | 3.788254440666737 | ||||
175 | 11.024193521677873 | 7.82040363764121 | 0.05354197316843104 | -0.004179901594152797 | 3.816078848019121 | ||||
180 | 11.043321748187033 | 7.754004351464536 | -0.04532988192544245 | 0.005261860743327792 | 3.803431366891105 | ||||
185 | 10.963965963231441 | 7.732109721563894 | -0.0810804831228705 | 0.013814656697474254 | 3.834276832710899 | ||||
190 | 11.140614517783744 | 7.813940392464405 | 0.07891811029757223 | 0.022848444863969534 | 3.818431989860988 | ||||
195 | 10.974995852192574 | 7.845978363660855 | -0.11349021944059817 | 0.012080010475507648 | 3.9668991529224993 | ||||
200 | 10.936095611343038 | 7.829326245465563 | 0.058960896406262335 | 0.01726789642127613 | 3.8754035482551914 | ||||
205 | 10.994549197718237 | 7.747143126684003 | 0.013581637256546264 | -0.017621451912855128 | 3.7374382859072193 | ||||
210 | 10.99507496943916 | 7.787741129777198 | 0.0770959235242969 | 0.07825500673833555 | 3.809634240646123 | ||||
215 | 11.090736436332357 | 7.781582553447613 | -0.03409288999570278 | 0.023836212104037836 | 3.7718677421416302 | ||||
220 | 11.090736436332357 | 7.781582553447613 | -0.03409288999570278 | 0.023836212104037836 | 3.7718677421416302 | ||||
225 | 11.16001631535962 | 7.791972210383601 | -0.07373837776379119 | 8.426754807035015E-4 | 3.8699203122478787 | ||||
230 | 11.187542707253526 | 7.812732327166721 | 0.06497892043908036 | 0.006796894860225541 | 3.8245548297085374 | ||||
235 | 11.01521661284773 | 7.7179493357699025 | -0.06718057279096754 | -0.014330502445702664 | 3.8036931637005154 | ||||
240 | 10.959058757774852 | 7.814706209969047 | 0.060590268146943495 | -0.01765690907969969 | 3.8516670423989052 | ||||
245 | 10.959058757774852 | 7.814706209969047 | 0.060590268146943495 | -0.01765690907969969 | 3.8516670423989052 | ||||
250 | 11.165915676954157 | 7.805639169747895 | -0.15702687042779323 | 0.007172891546869576 | 3.824257403405109 | ||||
255 | 11.043153610278939 | 7.7253216657547945 | 0.009511174347290617 | 0.019621840895865005 | 3.791211738310932 | ||||
260 | 11.083541148537648 | 7.742410825159817 | -0.0633641771003894 | -9.479974550892709E-4 | 3.8412394442434286 | ||||
265 | 11.134147862003566 | 7.803485727533137 | -0.15475333409066686 | 0.02707629133544473 | 3.8272076896203555 | ||||
270 | 11.11678957602749 | 7.778048878683464 | -0.0474500309151668 | -0.015340532045746716 | 3.789082492727709 | ||||
275 | 11.074792064280876 | 7.77409515588984 | 0.07610983815199526 | 0.012785363824594064 | 3.783495773019874 | ||||
280 | 11.006937804885222 | 7.772603697317924 | -0.12793734878720853 | -0.0189104624653826 | 3.8376767384481134 | ||||
285 | 10.96738622738075 | 7.764168527626137 | 0.047527322494908306 | 0.027275307795717886 | 3.824210988270692 | ||||
290 | 10.998881673293672 | 7.748680728313268 | -0.013992254504514127 | 0.028152551581285858 | 3.7784519748168277 | ||||
295 | 11.068049982195202 | 7.7750762108776446 | -0.2086692836983695 | 0.002571820024728194 | 3.84442881509923 | ||||
300 | 10.920678803994262 | 7.72186619852175 | -0.011942909657440371 | 0.038121226304865474 | 3.785648278543081 |