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MetadataKGSADH
KGSADH act
inhNADH
inhaKG
Inhibition on KGSADH
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| Metadata | Values (examples) | ||||||||
| Asset Title | KGSADH Kinetic Data | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | Computational pathway design for biotechnology | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 972 | ||||||||
| Assay Title | KGSADH | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of α-ketoglutarate semialdehyde dehydrogenase. Measures enzyme reaction rate with increasing concentration of NAD and α-ketoglutarate semialdehyde and check for product inhibition. | ||||||||
| Experimentalist | Lu Shen | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | degrees celcius | ||||||||
| Start_value (optional) | 37 | ||||||||
| End_value (optional) | 37 | ||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | ||||||||
| Compound (if concentration) | α-ketoglutarate semialdehyde | NAD | |||||||
| Unit | mM | mM | |||||||
| Start_value (optional) | 0 | 0 | |||||||
| End_value (optional) | 1 | 8 | |||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | α-ketoglutarate semialdehyde dehydrogenase | ||||||||
| EC Number | |||||||||
| Reaction | α-ketoglutarate semialdehyde + NAD(P) -> α-ketoglutarate + NAD(P)H |
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| Protein µg | NAD+ mM | KGSA mM | Specific activity U/mg | Standard deviation U/mg | |||||
| 0.13239 | 5 | 0.005 | 4.999195675966951 | 0.24539281924807177 | |||||
| 0.13239 | 5 | 0.01 | 10.342465548255516 | 0.34526271176515894 | |||||
| 0.13239 | 5 | 0.015 | 15.402380200043929 | 0.6660662236733403 | |||||
| 0.13239 | 5 | 0.025 | 25.44125086919213 | 0.27824930282203847 | |||||
| 0.13239 | 5 | 0.05 | 34.56933690101843 | 0.8507881993797333 | |||||
| 0.13239 | 5 | 0.1 | 40.722193117593136 | 0.12639662748994096 | |||||
| 0.13239 | 5 | 0.25 | 41.53177946187929 | 0.5464707823931505 | |||||
| 0.13239 | 5 | 0.5 | 41.53177946187929 | 1.3725712173422682 | |||||
| 0.13239 | 5 | 1 | 41.39010185162922 | 0.40428686403730585 | |||||
| Protein µg | NAD+ mM | KGSA mM | Specific activity U/mg | Standard deviation U/mg | |||||
| 0.13239 | 0.1 | 1 | 6.132616557967556 | 0.10516835110631541 | |||||
| 0.13239 | 0.25 | 1 | 13.54033160818579 | 0.48194192965838817 | |||||
| 0.13239 | 0.5 | 1 | 20.34085690018942 | 0.5564986056440774 | |||||
| 0.13239 | 1 | 1 | 30.480925862373397 | 0.26466787956103904 | |||||
| 0.13239 | 1.5 | 1 | 37.139773544126946 | 0.12639662748993982 | |||||
| 0.13239 | 3 | 1 | 41.531779461879296 | 0.3981609512078173 | |||||
| 0.13239 | 5 | 1 | 41.39010185162922 | 0.40428686403730585 | |||||
| 0.13239 | 8 | 1 | 41.47106048605783 | 0.39816095120781353 | |||||
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| CCNA00864 µg | NAD+ mM | KGSA [mM] | NADH mM | spez. mean [U/mg] | SD | Percentage % | |||
| 0.24396 | 5 | 2 | 0 | 29.216041777270252 | 0.27436710138372367 | 100 | |||
| 0.24396 | 5 | 2 | 0.02 | 28.776702803175965 | 0.6835392990690051 | 98.49624060150377 | |||
| 0.24396 | 5 | 2 | 0.05 | 26.865578265865803 | 0.6674663780138665 | 91.95488721804512 | |||
| 0.24396 | 5 | 2 | 0.08 | 25.767230830630083 | 0.610250375023664 | 88.19548872180452 | |||
| CCNA00865 µg | NAD+ mM | DOP [mM] | NADH mM | spez. mean [U/mg] | SD | Percentage % | |||
| 0.24396 | 1 | 2 | 0 | 14.179665388893193 | 0.2802400711188178 | 100 | |||
| 0.24396 | 1 | 2 | 0.02 | 14.047863696664907 | 0.10066520522656675 | 99.07048799380325 | |||
| 0.24396 | 1 | 2 | 0.04 | 13.553607350808832 | 0.19861571889857374 | 95.58481797056547 | |||
| 0.24396 | 1 | 2 | 0.08 | 13.311970915056973 | 0.0871786250033812 | 93.88071262587144 | |||
| 0.24396 | 0.5 | 2 | 0 | 8.676944738362218 | 0.10592079079636776 | 100 | |||
| 0.24396 | 0.5 | 2 | 0.02 | 8.46825872566743 | 0.3487145000135378 | 97.59493670886076 | |||
| 0.24396 | 0.5 | 2 | 0.04 | 8.138754495096713 | 0.1318016922283469 | 93.79746835443036 | |||
| 0.24396 | 0.5 | 2 | 0.08 | 7.842200687583069 | 0.15099780783980635 | 90.37974683544304 | |||
| 0.24396 | 0.05 | 2 | 0 | 1.5047359862729417 | 0.06859177534592889 | 100 | |||
| 0.24396 | 0.05 | 2 | 0.02 | 1.383917768397012 | 0.06590084611413975 | 91.97080291970804 | |||
| 0.24396 | 0.05 | 2 | 0.04 | 1.2301491274640106 | 0.05033260261328251 | 81.75182481751824 | |||
| 0.24396 | 0.05 | 2 | 0.08 | 1.0983474352357239 | 0.0380478712438256 | 72.99270072992701 | |||
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| CCNA00865 µg | NAD+ mM | KGSA [mM] | α-ketoglutarate mM | spez. mean [U/mg] | SD | Percentage % | |||
| 0.24396 | 5 | 2 | 0 | 28.469165521309964 | 0.314327002574135 | 100 | |||
| 0.24396 | 5 | 2 | 2 | 25.009371100317434 | 0.45299342351954924 | 87.84722222222221 | |||
| 0.24396 | 5 | 2 | 5 | 22.9884118194837 | 0.16584682576867749 | 80.74845679012346 | |||
| 0.24396 | 5 | 2 | 10 | 20.51713009020332 | 0.4497863501036782 | 72.06790123456788 | |||
| 0.13239 | 5 | 1 | 0 | 23.558962618726852 | 100 | ||||
| 0.13239 | 5 | 1 | 5 | 18.215692746438286 | 77.31958762886597 | ||||
| 0.13239 | 5 | 1 | 10 | 13.176017753257026 | 55.92783505154638 | ||||
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| KGSADH µg | KGSAmM | KDX mM | NADH mM | KG mM | Specific activity U/mg | |||||||
| 0.2915 | 1 | 0 | 0 | 0 | 46.93541003678722 | 45.58415557626867 | 46.259782806527944 | Black is the 1st replicate of measuremnet | ||||
| 0.2915 | 1 | 0 | 0.04 | 0 | 41.06158962677801 | 39.87579489611887 | 40.46869226144844 | Green is the 2nd replicate of measurement | ||||
| 0.2915 | 1 | 2 | 0 | 0 | 37.58693529973031 | 38.248774219167956 | 37.91785475944913 | |||||
| 0.2915 | 1 | 2 | 0.04 | 0 | 35.9047613794929 | 34.30531732418525 | 35.10503935183908 | |||||
| 0.2915 | 1 | 0 | 0.08 | 0 | 40.51005719391329 | 39.296685841610916 | 39.9033715177621 | |||||
| 0.2915 | 1 | 5 | 0 | 0 | 31.685538268077856 | 30.885816240424024 | 31.285677254250942 | |||||
| 0.2915 | 1 | 5 | 0.08 | 0 | 26.66659312900895 | 27.411161913376322 | 27.03887752119264 | |||||
| 0.2915 | 1 | 0 | 0 | 2 | 44.64655044039865 | 39.57245205804327 | 42.109501249220955 | |||||
| 0.2915 | 1 | 0 | 0 | 5 | 37.58693529973031 | 35.93233800113616 | 36.75963665043324 | |||||
| 0.2915 | 1 | 2 | 0.04 | 2 | 30.996122726996962 | 32.54041353901816 | 31.76826813300756 | |||||
| 0.2915 | 1 | 5 | 0.08 | 5 | 24.129543937831265 | 23.32982191017743 | 23.729682924004347 | |||||
| 0.2915 | 1 | 0 | 0 | 0 | 45.58415557626867 | |||||||
| 0.2915 | 1 | 0 | 0.04 | 0 | 39.87579489611887 | |||||||
| 0.2915 | 1 | 2 | 0 | 0 | 38.248774219167956 | |||||||
| 0.2915 | 1 | 2 | 0.04 | 0 | 34.30531732418525 | |||||||
| 0.2915 | 1 | 0 | 0.08 | 0 | 39.296685841610916 | |||||||
| 0.2915 | 1 | 5 | 0 | 0 | 30.885816240424024 | |||||||
| 0.2915 | 1 | 5 | 0.08 | 0 | 27.411161913376322 | |||||||
| 0.2915 | 1 | 0 | 0 | 2 | 39.57245205804327 | |||||||
| 0.2915 | 1 | 0 | 0 | 5 | 35.93233800113616 | |||||||
| 0.2915 | 1 | 2 | 0.04 | 2 | 32.54041353901816 | |||||||
| 0.2915 | 1 | 5 | 0.08 | 5 | 23.32982191017743 | |||||||
| KDX mM | NADH mM | KG mM | Specific activity U/mg | |||||||||
| 0 | 0 | 0 | 46.93541003678722 | 45.58415557626867 | 46.259782806527944 | |||||||
| 0 | 0.04 | 0 | 41.06158962677801 | 39.87579489611887 | 40.46869226144844 | |||||||
| 0 | 0.08 | 0 | 40.51005719391329 | 39.296685841610916 | 39.9033715177621 | |||||||
| 0 | 0 | 0 | 46.93541003678722 | 45.58415557626867 | 46.259782806527944 | |||||||
| 0 | 0 | 2 | 44.64655044039865 | 39.57245205804327 | 42.109501249220955 | |||||||
| 0 | 0 | 5 | 37.58693529973031 | 35.93233800113616 | 36.75963665043324 | |||||||
| 0 | 0 | 0 | 46.93541003678722 | 45.58415557626867 | 46.259782806527944 | |||||||
| 2 | 0 | 0 | 37.58693529973031 | 38.248774219167956 | 37.91785475944913 | |||||||
| 5 | 0 | 0 | 31.685538268077856 | 30.885816240424024 | 31.285677254250942 | |||||||
| 2 | 0.04 | 0 | 35.9047613794929 | 34.30531732418525 | 35.10503935183908 | |||||||
| 5 | 0.08 | 0 | 26.66659312900895 | 27.411161913376322 | 27.03887752119264 | |||||||
| 2 | 0.04 | 2 | 30.996122726996962 | 32.54041353901816 | 31.76826813300756 | |||||||
| 5 | 0.08 | 5 | 24.129543937831265 | 23.32982191017743 | 23.729682924004347 |
