KGSADH-initial rate kinetics data

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MetadataKGSADH KGSADH act inhNADH inhaKG Inhibition on KGSADH

Metadata	Values (examples)

Asset Title	KGSADH Kinetic Data
Uploader	Jacky Snoep
Uploader SEEK ID	49
Project	Computational pathway design for biotechnology

ASSAY
Assay SEEK ID	972
Assay Title	KGSADH
Assay_type	enzymaticAssay
Technology_type	initial rate experiment
Description	Kinetic characterisation of α-ketoglutarate semialdehyde dehydrogenase. Measures enzyme reaction rate with increasing concentration of NAD and α-ketoglutarate semialdehyde and check for product inhibition.
Experimentalist	Lu Shen
Date
SOP
Publication (optional)

Experimental_conditions
Item	temperature
Compound (if concentration)
Unit	degrees celcius
Start_value (optional)	37
End_value (optional)	37
Comments

Culture growth	CultureGrowth

FACTORS_STUDIED
Item	concentration
Compound (if concentration)	α-ketoglutarate semialdehyde	NAD
Unit	mM	mM
Start_value (optional)	0	0
End_value (optional)	1	8
SD (optional)
Comments

ENZYME IDENTITY

Enzyme Name (IUBMB)	α-ketoglutarate semialdehyde dehydrogenase
EC Number

Reaction	α-ketoglutarate semialdehyde + NAD(P) -> α-ketoglutarate + NAD(P)H

Protein µg	NAD+ mM	KGSA mM	Specific activity U/mg	Standard deviation U/mg
0.13239	5	0.005	4.999195675966951	0.24539281924807177
0.13239	5	0.01	10.342465548255516	0.34526271176515894
0.13239	5	0.015	15.402380200043929	0.6660662236733403
0.13239	5	0.025	25.44125086919213	0.27824930282203847
0.13239	5	0.05	34.56933690101843	0.8507881993797333
0.13239	5	0.1	40.722193117593136	0.12639662748994096
0.13239	5	0.25	41.53177946187929	0.5464707823931505
0.13239	5	0.5	41.53177946187929	1.3725712173422682
0.13239	5	1	41.39010185162922	0.40428686403730585

Protein µg	NAD+ mM	KGSA mM	Specific activity U/mg	Standard deviation U/mg
0.13239	0.1	1	6.132616557967556	0.10516835110631541
0.13239	0.25	1	13.54033160818579	0.48194192965838817
0.13239	0.5	1	20.34085690018942	0.5564986056440774
0.13239	1	1	30.480925862373397	0.26466787956103904
0.13239	1.5	1	37.139773544126946	0.12639662748993982
0.13239	3	1	41.531779461879296	0.3981609512078173
0.13239	5	1	41.39010185162922	0.40428686403730585
0.13239	8	1	41.47106048605783	0.39816095120781353

CCNA00864 µg	NAD+ mM	KGSA [mM]	NADH mM	spez. mean [U/mg]	SD	Percentage %
0.24396	5	2	0	29.216041777270252	0.27436710138372367	100
0.24396	5	2	0.02	28.776702803175965	0.6835392990690051	98.49624060150377
0.24396	5	2	0.05	26.865578265865803	0.6674663780138665	91.95488721804512
0.24396	5	2	0.08	25.767230830630083	0.610250375023664	88.19548872180452

CCNA00865 µg	NAD+ mM	DOP [mM]	NADH mM	spez. mean [U/mg]	SD	Percentage %
0.24396	1	2	0	14.179665388893193	0.2802400711188178	100
0.24396	1	2	0.02	14.047863696664907	0.10066520522656675	99.07048799380325
0.24396	1	2	0.04	13.553607350808832	0.19861571889857374	95.58481797056547
0.24396	1	2	0.08	13.311970915056973	0.0871786250033812	93.88071262587144
0.24396	0.5	2	0	8.676944738362218	0.10592079079636776	100
0.24396	0.5	2	0.02	8.46825872566743	0.3487145000135378	97.59493670886076
0.24396	0.5	2	0.04	8.138754495096713	0.1318016922283469	93.79746835443036
0.24396	0.5	2	0.08	7.842200687583069	0.15099780783980635	90.37974683544304
0.24396	0.05	2	0	1.5047359862729417	0.06859177534592889	100
0.24396	0.05	2	0.02	1.383917768397012	0.06590084611413975	91.97080291970804
0.24396	0.05	2	0.04	1.2301491274640106	0.05033260261328251	81.75182481751824
0.24396	0.05	2	0.08	1.0983474352357239	0.0380478712438256	72.99270072992701

CCNA00865 µg	NAD+ mM	KGSA [mM]	α-ketoglutarate mM	spez. mean [U/mg]	SD	Percentage %
0.24396	5	2	0	28.469165521309964	0.314327002574135	100
0.24396	5	2	2	25.009371100317434	0.45299342351954924	87.84722222222221
0.24396	5	2	5	22.9884118194837	0.16584682576867749	80.74845679012346
0.24396	5	2	10	20.51713009020332	0.4497863501036782	72.06790123456788


0.13239	5	1	0	23.558962618726852		100
0.13239	5	1	5	18.215692746438286		77.31958762886597
0.13239	5	1	10	13.176017753257026		55.92783505154638

KGSADH µg	KGSAmM	KDX mM	NADH mM	KG mM	Specific activity U/mg
0.2915	1	0	0	0	46.93541003678722	45.58415557626867	46.259782806527944	Black is the 1st replicate of measuremnet
0.2915	1	0	0.04	0	41.06158962677801	39.87579489611887	40.46869226144844	Green is the 2nd replicate of measurement
0.2915	1	2	0	0	37.58693529973031	38.248774219167956	37.91785475944913
0.2915	1	2	0.04	0	35.9047613794929	34.30531732418525	35.10503935183908
0.2915	1	0	0.08	0	40.51005719391329	39.296685841610916	39.9033715177621
0.2915	1	5	0	0	31.685538268077856	30.885816240424024	31.285677254250942
0.2915	1	5	0.08	0	26.66659312900895	27.411161913376322	27.03887752119264
0.2915	1	0	0	2	44.64655044039865	39.57245205804327	42.109501249220955
0.2915	1	0	0	5	37.58693529973031	35.93233800113616	36.75963665043324
0.2915	1	2	0.04	2	30.996122726996962	32.54041353901816	31.76826813300756
0.2915	1	5	0.08	5	24.129543937831265	23.32982191017743	23.729682924004347
0.2915	1	0	0	0	45.58415557626867
0.2915	1	0	0.04	0	39.87579489611887
0.2915	1	2	0	0	38.248774219167956
0.2915	1	2	0.04	0	34.30531732418525
0.2915	1	0	0.08	0	39.296685841610916
0.2915	1	5	0	0	30.885816240424024
0.2915	1	5	0.08	0	27.411161913376322
0.2915	1	0	0	2	39.57245205804327
0.2915	1	0	0	5	35.93233800113616
0.2915	1	2	0.04	2	32.54041353901816
0.2915	1	5	0.08	5	23.32982191017743
KDX mM	NADH mM	KG mM	Specific activity U/mg
0	0	0	46.93541003678722	45.58415557626867	46.259782806527944
0	0.04	0	41.06158962677801	39.87579489611887	40.46869226144844
0	0.08	0	40.51005719391329	39.296685841610916	39.9033715177621
0	0	0	46.93541003678722	45.58415557626867	46.259782806527944
0	0	2	44.64655044039865	39.57245205804327	42.109501249220955
0	0	5	37.58693529973031	35.93233800113616	36.75963665043324
0	0	0	46.93541003678722	45.58415557626867	46.259782806527944
2	0	0	37.58693529973031	38.248774219167956	37.91785475944913
5	0	0	31.685538268077856	30.885816240424024	31.285677254250942
2	0.04	0	35.9047613794929	34.30531732418525	35.10503935183908
5	0.08	0	26.66659312900895	27.411161913376322	27.03887752119264
2	0.04	2	30.996122726996962	32.54041353901816	31.76826813300756
5	0.08	5	24.129543937831265	23.32982191017743	23.729682924004347