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| Metadata | Values (examples) | ||||||||
| Asset Title | KDXD Kinetic Data | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | Computational pathway design for biotechnology | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 970 | ||||||||
| Assay Title | KDXD | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of kdXylonate dehydratase. Measures enzyme reaction rate with increasing concentration of kdXylonate, and check for inhibition with pathway intermediates. The activity was measured using a linked assay with KGSADH. | ||||||||
| Experimentalist | Lu Shen | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | degrees celcius | ||||||||
| Start_value (optional) | 37 | ||||||||
| End_value (optional) | 37 | ||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | ||||||||
| Compound (if concentration) | 2-keto-3-deoxy-D-xylonate | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 0 | ||||||||
| End_value (optional) | 10 | ||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | 2-keto-3-deoxy-D-xylonate dehydratase | ||||||||
| EC Number | |||||||||
| Reaction | 2-keto-3-deoxy-D-xylonate -> α-ketoglutarate semialdehyde + H2O |
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| CCNA00866 [µg] | CCNA00865 [µg] | NAD [mM] | 2-KDX [mM] | Mean spec. act. [U/mg] | SD | ||||
| 0.1419 | 24.396 | 5 | 0.05 | 14.445660523578717 | 0.42769547161233606 | ||||
| 0.1419 | 24.396 | 5 | 0.1 | 26.663101515415878 | 1.164192787200379 | ||||
| 0.1419 | 24.396 | 5 | 0.2 | 55.629955428056086 | 0.653315624351121 | ||||
| 0.1419 | 24.396 | 5 | 0.5 | 84.21914501328247 | 0.8038130748067227 | ||||
| 0.1419 | 24.396 | 5 | 1 | 92.18786232171412 | 0.5702606288164459 | ||||
| 0.1419 | 24.396 | 5 | 2 | 97.58846220373177 | 1.7863362997026773 | ||||
| 0.1419 | 24.396 | 5 | 3 | 98.40044050767149 | 2.398094134553881 | ||||
| 0.1419 | 24.396 | 5 | 5 | 97.05973214535243 | 1.2363821067831429 | ||||
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| CCNA00866 [µg] | CCNA00865 [µg] | NAD+ [mM] | 2-KDX [mM] | XA [mM] | mean spec. act. [U/mg] | SD | Percentage % | ||
| 0.1419 | 37.51 | 5 | 2 | 0 | 99.36348454257676 | 1.7451402306263073 | 100 | ||
| 0.1419 | 37.51 | 5 | 2 | 1 | 94.73709653175742 | 0.9382887612991423 | 95.3439756746484 | ||
| 0.1419 | 24.39 | 5 | 2 | 0 | 99.04246986427499 | 1.7045175597139097 | 100 | ||
| 0.1419 | 24.39 | 5 | 2 | 2 | 87.39152536355857 | 0.8653376838292168 | 88.2364156339371 | ||
| 0.1419 | 37.51 | 5 | 2 | 0 | 103.00794530212012 | 1.1076901805056003 | 100 | ||
| 0.1419 | 37.51 | 5 | 2 | 5 | 81.7832101014633 | 0.6638733038621787 | 79.39505041246564 | ||
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| CCNA00866 [µg] | CCNA00865 [µg] | NAD+ [mM] | 2-KDX [mM] | aKG [mM] | mean spec. act. [U/mg] | SD | Percentage % | ||
| 0.1419 | 37.51 | 5 | 2 | 0 | 99.36348454257676 | 1.7451402306263073 | 100 | ||
| 0.1419 | 37.51 | 5 | 2 | 1 | 94.94481191183502 | 1.4252763511971336 | 95.55302166476623 | ||
| 0.1419 | 24.39 | 5 | 2 | 0 | 99.04246986427499 | 1.7045175597139097 | 100 | ||
| 0.1419 | 24.39 | 5 | 2 | 2 | 89.48756238070528 | 2.0151143638514926 | 90.35271687321259 | ||
| 0.1419 | 37.51 | 5 | 2 | 0 | 103.00794530212012 | 1.1076901805056003 | 100 | ||
| 0.1419 | 37.51 | 5 | 2 | 5 | 75.07966828986795 | 1.0202180829637915 | 72.88725939505044 | ||
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| CCNA00866 [µg] | CCNA00865 [µg] | NAD+ [mM] | 2-KDX [mM] | Pyr [mM] | mean spec. act. [U/mg] | SD | Percentage % | ||
| 0.1419 | 37.51 | 5 | 2 | 0 | 99.36348454257676 | 1.7451402306263073 | 100 | ||
| 0.1419 | 37.51 | 5 | 2 | 1 | 95.83232308125748 | 0.7712123801637942 | 96.44621816799693 | ||
| 0.1419 | 24.39 | 5 | 2 | 0 | 99.04246986427499 | 1.7045175597139097 | 100 | ||
| 0.1419 | 24.39 | 5 | 2 | 2 | 88.07132115290345 | 1.1447341557523754 | 88.92278360343184 | ||
| 0.1419 | 37.51 | 5 | 2 | 0 | 103.00794530212012 | 1.1076901805056003 | 100 | ||
| 0.1419 | 37.51 | 5 | 2 | 5 | 80.57468425373906 | 1.7007478885463747 | 78.22181484876262 | ||
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| CCNA00866 [µg] | CCNA00865 [µg] | NAD+ [mM] | 2-KDX [mM] | Lac [mM] | mean spec. act. [U/mg] | SD | Percentage % | ||
| 0.1419 | 37.51 | 5 | 2 | 0 | 99.36348454257676 | 1.7451402306263073 | 100 | ||
| 0.1419 | 37.51 | 5 | 2 | 1 | 95.53019161932644 | 0.8855000379698724 | 96.1421512732801 | ||
| 0.1419 | 24.39 | 5 | 2 | 0 | 99.04246986427499 | 1.7045175597139097 | 100 | ||
| 0.1419 | 24.39 | 5 | 2 | 2 | 93.32085530395558 | 1.5558899437140097 | 94.2230695900858 | ||
| 0.1419 | 37.51 | 5 | 2 | 0 | 103.00794530212012 | 1.1076901805056003 | 100 | ||
| 0.1419 | 37.51 | 5 | 2 | 5 | 87.05162746888612 | 1.7241731203704551 | 84.50962419798351 | ||
