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| Metadata | Values (examples) | ||||||||
| Asset Title | XAD Kinetic Data | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | Computational pathway design for biotechnology | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 970 | ||||||||
| Assay Title | XAD | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of Xylonate dehydratase. Measures enzyme reaction rate with increasing concentration of Xylonate. The activity was measured using a linked assay with KDXD, and KGSADH. | ||||||||
| Experimentalist | Lu Shen | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | degrees celcius | ||||||||
| Start_value (optional) | 37 | ||||||||
| End_value (optional) | 37 | ||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | ||||||||
| Compound (if concentration) | D-Xylonate | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 0 | ||||||||
| End_value (optional) | 10 | ||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | D-Xylonate dehydratase | ||||||||
| EC Number | 4.2.1.82 | ||||||||
| Reaction | D-xylonate -> 2-keto-3-deoxy-D-xylonate + H2O |
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| Protein µg | D-xylonate mM | Specific activity U/mg | Standard deviation U/mg | ||||||
| 3.2888 | 0.15 | 6.9630260277304785 | 0.030406227195329816 | ||||||
| 3.2888 | 0.25 | 9.973242520068109 | 0.35848413167573534 | ||||||
| 3.2888 | 0.5 | 16.358550231087325 | 0.08044731546657218 | ||||||
| 3.2888 | 1 | 22.956701532473854 | 0.6385307711019212 | ||||||
| 3.2888 | 2 | 29.960269196464765 | 0.8644856335255009 | ||||||
| 3.2888 | 3 | 34.8252655477175 | 0.6144265209692585 | ||||||
| 3.2888 | 5 | 37.703721722208705 | 0.08044731546656815 | ||||||
| 3.2888 | 7.5 | 37.57196140436228 | 0.517203876679358 | ||||||
| 3.2888 | 10 | 38.43347117489662 | 0.7764014433716686 | ||||||
