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| Metadata | Values (examples) | ||||||||
| Asset Title | XLA Kinetic Data | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | Computational pathway design for biotechnology | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 969 | ||||||||
| Assay Title | XLA | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of Xylonolactonase. Measures enzyme reaction rate with increasing concentration of Xylonolactone. The activity was measured using a linked assay with XAD, KDXD, and KGSADH (0.3 U each). | ||||||||
| Experimentalist | Lu Shen | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | degrees celcius | ||||||||
| Start_value (optional) | 37 | ||||||||
| End_value (optional) | 37 | ||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | |||||||
| Compound (if concentration) | D-Xylono-1,4-lactone | NAD | |||||||
| Unit | mM | mM | |||||||
| Start_value (optional) | 0 | 5 | |||||||
| End_value (optional) | 2 | 5 | |||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | D-Xylono-1,4-lactonase | ||||||||
| EC Number | 3.1.1.68 | ||||||||
| Reaction | D-xylono-1,4-lactone + H2O -> D-xylonate |
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| XLA (ug) | NAD mM | D-xylonolactone mM | Control (without lactonase) uM/min | With lactonase U/mg | Standard deviation U/mg | ||||
| 0.0069 | 5 | 0.15 | 0.9967845659163986 | 334.3585442005686 | 9.099037322226897 | ||||
| 0.0069 | 5 | 0.25 | 1.5916398713826367 | 538.6240427481864 | 8.744062513939475 | ||||
| 0.0069 | 5 | 0.35 | 2.282958199356913 | 733.1811050530468 | 4.850333192287943 | ||||
| 0.0069 | 5 | 0.5 | 3.15112540192926 | 984.0470975659009 | 15.160089236795068 | ||||
| 0.0069 | 5 | 0.75 | 5.594855305466237 | 1460.148810910729 | 6.416387701297994 | ||||
| 0.0069 | 5 | 1 | 7.242765273311896 | 1743.2468117495375 | 12.125832980719867 | ||||
| 0.0069 | 5 | 1.5 | 10.980707395498392 | 2312.937850474548 | 12.832775402595823 | ||||
| 0.0069 | 5 | 2 | 14.284565916398716 | 2789.4279012690868 | 18.060822893631883 | ||||
| 0.3 U of XAD, KDXD, and KGSADH | |||||||||
