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| Metadata | Values (examples) | ||||||||
| Asset Title | XDH Kinetic Data | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | Computational pathway design for biotechnology | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 968 | ||||||||
| Assay Title | XDH | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of Xylose dehydrogenase. Measures enzyme reaction rate with increasing concentration of XYL, NAD, XLAC, NADH | ||||||||
| Experimentalist | Lu Shen | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | degrees celcius | ||||||||
| Start_value (optional) | 37 | ||||||||
| End_value (optional) | 37 | ||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | concentration | concentration | |||||
| Compound (if concentration) | Xyl | NAD | XLAC | NADH | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | 0 | 0 | 0 | 0 | |||||
| End_value (optional) | 5 | 3 | 10 | 0.08 | |||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Xylose dehydrogenase | ||||||||
| EC Number | 1.1.1.175 | ||||||||
| Reaction | Xylose + NAD+ -> Xylonolactone + NADH |
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| Protein µg | NAD+ mM | D-xylose mM | Specific activity U/mg | Standard deviation U/mg | |||||
| 0.136 | 3 | 0.03 | 11.506210201122249 | 0.474087755166603 | |||||
| 0.136 | 3 | 0.05 | 20.904262026353948 | 0.33609801772491943 | |||||
| 0.136 | 3 | 0.1 | 35.52345375449215 | 0.6816579933487071 | |||||
| 0.136 | 3 | 0.15 | 47.404009835445436 | 0.2576425042285742 | |||||
| 0.136 | 3 | 0.25 | 65.31350482315112 | 2.061140033828571 | |||||
| 0.136 | 3 | 0.5 | 82.96686841939346 | 1.5775471377187777 | |||||
| 0.136 | 3 | 1 | 102.07821070550405 | 2.231249537565835 | |||||
| 0.136 | 3 | 2 | 102.51166382951892 | 3.93155749456089 | |||||
| 0.136 | 3 | 5 | 102.1373179496879 | 1.3308977502834451 | |||||
| Protein µg | NAD+ mM | D-xylose mM | Specific activity U/mg | Standard deviation U/mg | |||||
| 0.136 | 0.01 | 2 | 6.028938906752411 | 0.40950700008721375 | |||||
| 0.136 | 0.05 | 2 | 30.637254901960787 | 0.729521648287217 | |||||
| 0.136 | 0.15 | 2 | 49.275739234600586 | 0.6721960354498447 | |||||
| 0.136 | 0.25 | 2 | 62.377845028686714 | 3.744029087928424 | |||||
| 0.136 | 0.5 | 2 | 84.18841813252631 | 3.362539033350729 | |||||
| 0.136 | 1 | 2 | 99.83213542651788 | 0.8924998150263398 | |||||
| 0.136 | 2 | 2 | 100.81725616291533 | 2.3953631550733636 | |||||
| 0.136 | 3 | 2 | 101.21130445747433 | 0.7484324724647985 | |||||
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| Protein µg | NAD+ mM | D-xylose mM | XLAC mM | Specific activity U/mg | fraction act | ||||
| 0.13365 | 3 | 0 | 0 | 0 | 0 | ||||
| 0.13365 | 3 | 2 | 0 | 49.86148251600199 | 1 | ||||
| 0.13365 | 3 | 2 | 5 | 27.847848498080726 | 0.5585042219541616 | ||||
| 0.13365 | 3 | 2 | 10 | 21.051289361400112 | 0.422195416164053 | ||||
| 0.1309 | 3 | 5 | 0 | 105.80964335456486 | 1 | ||||
| 0.1309 | 3 | 5 | 2 | 89.67925082924138 | 0.8475527181272975 | ||||
| 0.1309 | 3 | 5 | 5 | 78.03179734331619 | 0.7374733991100796 | ||||
| 0.1309 | 3 | 5 | 10 | 57.00906495307857 | 0.538788934029793 | ||||
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| Protein µg | NAD+ mM | D-xylose mM | NADH mM | Specific activity U/mg | SD (U/mg) | fraction act | |||
| 0.1309 | 3 | 5 | 0 | 93.09774772229851 | 0.8920335745318775 | 1 | |||
| 0.1309 | 3 | 5 | 0.02 | 90.94839338834043 | 1.0439721050653503 | 0.9769129287598945 | |||
| 0.1309 | 3 | 5 | 0.05 | 84.15233968477774 | 1.1753797168056395 | 0.9039138082673703 | |||
| 0.1309 | 3 | 5 | 0.08 | 82.658026671645 | 1.260030393631726 | 0.8878627968337733 | |||
