Selected Cell
Cell:
Value:
miRNA assays
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
| Table xxx: RT-qPCR assays for selected miRNAs used for sRNA-Seq results validation. | |||||||||||||||
| TaqMan MicroRNA Assays (Thermo Fisher Scientific), ordered according to the sRNA-Seq sequence of the selected miRNAs together with their IDs, mature miRNA sequence and efficiency of amplification. | |||||||||||||||
| Assay name | qPCR chemistry | Assay ID | Potato target miRNA ID | Target miRNA sequence | Efficiency of amplification | ||||||||||
| gma-miR390b | Taqman | 007109_mat | stu-miR390-5p | AAGCTCAGGAGGGATAGCACC | 1.065 | ||||||||||
| stu-miR398a-5p | Taqman | 469260_mat | stu-miR398a-5p | GGGTTGATTTGAGAACATATG | 0.916 | ||||||||||
| stu-miR408b-5p | Taqman | 468849_mat | stu-miR408b-5p | ACGGGGACGAGACAGAGCATG | 1.042 | ||||||||||
| sly-miR4376 | Taqman | 473102_mat | stu-miR4376-5p.1 | ACGCAGGAGAGATGATGCTGGA | 1.046 | ||||||||||
| stu-miR6022 | Taqman | 475531_mat | stu-miR6022-3p | TGGAAGGGAGAATATCCAGGA | 1.007 | ||||||||||
| stu-miR827-5p | Taqman | 468282_mat | stu-miR827-5p | TTTGTTGATGGTCATCTATTC | 1.056 | ||||||||||
| bna-miR167a | Taqman | 006933_mat | stu-miR167a-5p.1 | TGAAGCTGCCAGCATGATCTAA | 0.983 | ||||||||||
