MOA2010-05_2_transcriptomics_qPCR

Selected Cell

Cell:

Value:

qPCR assays

Assay name	qPCR chemistry	Reference	Target gene annotation		Sequence1	Primers/probe concentration (nM)	Efficiency of amplification	Gene target
RA	SybrGreen	Kogovšek et al. 2010	RUBISCO ACTIVASE	FW:	AGAGGCAGCACTCGGAGATGC	300	0.93	Sotub10g028070.1.1, PGSC0003DMG400019149
				RW:	GCAACAGGACGCGCTTTTTTCCC	300
CAB	SybrGreen	Kogovšek et al. 2010	CHLOROPHYLL A-B BINDING PROTEIN 4	FW:	GGGCCTGCCAAGTTGTTCTTATGG	300	0.83	Sotub07g017980.1.1, PGSC0003DMG400006149
				RW:	CAATTCAGCAAAAGCCTCGGGATCATC	300
CAB_NEW	TaqMan	Stare et al., 2015, BMC genomics	Chlorophyll A/B Binding Protein (Whole Family Of Cab Proteins)	FW:	TTGGTCCATGCACAAAGCAT	900	1.01	PGSC0003DMG400042498, PGSC0003DMG400016695, PGSC0003DMG400013460, PGSC0003DMG401013418, PGSC0003DMG400013415, PGSC0003DMG400013414, PGSC0003DMG400013413, PGSC0003DMG400013412, PGSC0003DMG400013411, PGSC0003DMG400008299, PGSC0003DMG400008298
				RW:	ACGGCTCCCATCAACACAA	900
				P:	FAM-TTGGCCATTTGGGCTTGCCAA- Zen Iowa BlackTM FQ	250
GBSS1	SybrGreen	Kogovšek et al., 2010, PLant Pathology	Granule-Bound Starch Synthase 1	FW:	CCAAGAAATGGGAGACATTGCTATTGGG	300	0.93	TC125827, Sotub08g026990, MICRO.920.C5
				RW:	TGATCTTATTGTTGAAACAAGGATAACCAAAGCTC	300
GluI	SybrGreen	Oufir et al. 2008	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE, BASIC (I)	FW:	ACGCGAGATGGTGGGTACAG	300	0.93	Sotub01g017890.1.1, Sotub01g017930.1.1, PGSC0003DMG400020017, PGSC0003DMG400040260
				RW:	TCAGCCCTGTTACTGGCACA	300
GluII	SybrGreen	Kogovšek et al. 2010	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE, ACIDIC (II)	FW:	GATGCCCTTKTGGATTCWATGTA	300	0.97	Sotub01g009310.1.1, Sotub01g009300.1.1, Sotub01g009360.1.1, PGSC0003DMG402010492, PGSC0003DMG401010492, PGSC0003DMG400010490
				RW:	GTATCKGAAAGTGGYTGGCCTT	300
GluIII	SybrGreen	Kogovšek et al., 2010, Plant Pathology	Glucan Endo-1,3-Beta-Glucosidase, Acidic (Iii)	FW:	CCCTGGAGTTGTTGTAAATGATAAYG	300	0.93	TA28935, X54456
				RW:	ATGCYACATACTCRGCCCTTGAGA	300
INV	TaqMan	Petek et al., 2014, Molecular ecology	CELL WALL INVERTASE, INVERTASE_CW, BETA-FRUCTOFURANOSIDASE	FW:	CGCGGAGAGAATCACAATTGA	900	0.86	Sotub10g025820.1.1, PGSC0003DMG402028252
				RW:	TCTCCCAATGTTCTAGTGCAACTTT	900
				P:	FAM-CTAAATGCTTGGAGCATGGCTAATG-TAMRA	250
PR-1b	SybrGreen	Baebler et al. 2011	PR-1B; PATHOGENESIS RELATED PROTEIN 1B	FW:	GTATGAATAATTCCACGTACCATATGTTC	300	0.93	Sotub09g006090.1.1, Sotub09g006100.1.1, Sotub09g006110.1.1, PGSC0003DMG400002027, PGSC0003DMG400002028, PGSC0003DMG400002029
				RW:	GTGGAAACAAGAAGATGCAATACTTAGT	300