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| Metadata | Values (examples) | ||||||||
| Asset Title | PGK reaction 70C, 28 ug/ml | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | |||||||||
| Assay Title | Sso PGK reaction with ATP recycling | ||||||||
| Assay_type | Metabolite concentration | ||||||||
| Technology_type | progressive curve experiment | ||||||||
| Description | Progress curve of Sulfolobus solfataricus Phosphoglycerate kinase. The conversion of metabolites was followed using 13P-NMR | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | concentration | concentration | concentration | concentration | |||||
| Compound (if concentration) | 3PG | Pi | PEP | ATP | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | 5 | 0 | 5 | 10 | |||||
| End_value (optional) | 5 | 5 | 0 | 10 | |||||
| Comments | |||||||||
| Culture growth | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | enzymeActivity | ||||||||
| Compound (if concentration) | Sso PGK activity with ATP recycling system at 70C | ||||||||
| Unit | 28 ug/ml | ||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | ||||||||
| EC Number | 2.7.2.3 | ||||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | KEGG | CHEBI | |||||||
| 3PG | C00197 | 17794 | |||||||
| PEP | C00074 | 44897 | |||||||
| ATP | C00002 | 15422 | |||||||
| Pi | 24838 | ||||||||
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| time | 3PG | pi | atp | pep | |||||
| 7.467 | 5.378 | 0.813 | 10 | 4.707 | |||||
| 8.533 | 5.523 | 1.101 | 10.266 | 3.867 | |||||
| 9.6 | 5.685 | 1.234 | 9.89 | 3.971 | |||||
| 10.666 | 5.788 | 1.451 | 10.08 | 4.064 | |||||
| 11.733 | 5.071 | 1.822 | 9.746 | 3.227 | |||||
| 12.8 | 5.508 | 1.035 | 9.951 | 3.917 | |||||
| 13.866 | 5.405 | 1.848 | 10.104 | 3.403 | |||||
| 14.933 | 5.65 | 1.418 | 9.983 | 4.226 | |||||
| 16 | 5.141 | 1.991 | 10.168 | 3.476 | |||||
| 17.066 | 5.357 | 2.229 | 10.169 | 2.865 | |||||
| 18.133 | 5.589 | 2.197 | 9.981 | 3.135 | |||||
| 19.2 | 5.685 | 1.902 | 10.071 | 2.657 | |||||
| 20.266 | 5.947 | 2.791 | 9.992 | 3.493 | |||||
| 21.333 | 5.485 | 2.428 | 9.911 | 3.232 | |||||
| 22.4 | 5.55 | 2.465 | 10.181 | 3 | |||||
| 23.466 | 5.618 | 1.929 | 10.158 | 2.537 | |||||
| 24.533 | 5.737 | 2.715 | 10.289 | 1.834 | |||||
| 25.599 | 6.241 | 2.842 | 10.231 | 2.844 | |||||
| 26.666 | 5.586 | 3.353 | 10.014 | 2.491 | |||||
| 27.733 | 5.807 | 3.936 | 10.267 | 2.55 | |||||
| 28.799 | 5.818 | 3.832 | 10.209 | 2.247 | |||||
| 29.866 | 5.36 | 3.839 | 10.172 | 2.013 | |||||
| 30.933 | 5.306 | 3.74 | 10.284 | 1.689 | |||||
| 31.999 | 5.992 | 4.489 | 10.136 | 1.997 | |||||
| 33.066 | 5.869 | 3.655 | 10.589 | 1.311 | |||||
| 34.133 | 5.831 | 4.012 | 10.175 | 1.095 | |||||
| 35.199 | 5.461 | 4.044 | 10.142 | 1.196 | |||||
| 36.266 | 5.654 | 4.48 | 10.1 | 1.197 | |||||
| 37.333 | 6.166 | 4.566 | 9.751 | 1.084 | |||||
| 38.399 | 5.897 | 5.059 | 10.173 | 0.615 | |||||
| 39.466 | 6.161 | 4.164 | 10.333 | 1.588 | |||||
| 40.532 | 5.89 | 4.238 | 10.037 | 0.964 | |||||
| 41.599 | 5.859 | 4.713 | 9.873 | 0.949 | |||||
| 42.666 | 5.486 | 4.629 | 10.104 | 1.208 | |||||
| 43.732 | 5.598 | 4.937 | 10.236 | 0.86 | |||||
| 44.799 | 5.525 | 5.223 | 9.74 | 0.53 | |||||
| 45.866 | 5.486 | 5.181 | 9.823 | 0.824 | |||||
| 46.932 | 6.062 | 4.711 | 9.76 | 1.531 | |||||
| 47.999 | 6.177 | 5.046 | 10.092 | 0.714 | |||||
| 49.066 | 5.272 | 5.147 | 9.929 | 0.199 |
