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| Metadata | Values (examples) | ||||||||
| Asset Title | PGK-GAPDH reaction 30C | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | |||||||||
| Assay Title | yeast PGK-GAPDH reaction | ||||||||
| Assay_type | Metabolite concentration | ||||||||
| Technology_type | progressive curve experiment | ||||||||
| Description | Progress curve of yeast Phosphoglycerate kinase-Glyceraldehyde 3-phosphate couple. The production of Pyruvatevia ATP recycling system was followed over time | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | concentration | ||||||||
| Compound (if concentration) | PYR | ||||||||
| Unit | mM | ||||||||
| Start_value (optional) | 0 | ||||||||
| End_value (optional) | 3 | ||||||||
| Comments | |||||||||
| Culture growth | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | enzymeActivity | ||||||||
| Compound (if concentration) | yeast PGK-GAPDH activity at 30C | ||||||||
| Unit | ug/ml | ||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | Glyceraldehyde 3-phosphate | |||||||
| EC Number | 2.7.2.3 | 1.2.1.12 | |||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | KEGG | CHEBI | |||||||
| PYR | C00022 | 32816 | |||||||
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| time | Pyr | stdev | |||||||
| 0 | 0.156084656 | 0.200806721 | |||||||
| 20 | 0.631746032 | 0.145071029 | |||||||
| 40 | 1.225925926 | 0.14094249 | |||||||
| 60 | 1.65026455 | 0.061256955 | |||||||
| 80 | 2.051851852 | 0.126908046 | |||||||
| 100 | 2.45978836 | 0.124634344 | |||||||
| 120 | 2.803174603 | 0.237973514 | |||||||
| 150 | 3.092063492 | 0.096225045 | |||||||
