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| Metadata | Values (examples) | ||||||||
| Asset Title | PGK-GAPDH reaction 70C | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | |||||||||
| Assay Title | Sulfolobus solfataricus PGK-GAPDH reaction | ||||||||
| Assay_type | Metabolite concentration | ||||||||
| Technology_type | progressive curve experiment | ||||||||
| Description | Progress curve of yeast Phosphoglycerate kinase-Glyceraldehyde 3-phosphate couple. The conversion of 3PG to GAP was followed over time | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | concentration | concentration | concentration | concentration | |||||
| Compound (if concentration) | 3PG | GAP | BPG | PYR | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | 3 | 0 | 0 | 0 | |||||
| End_value (optional) | 0 | 0 | 0 | 3 | |||||
| Comments | |||||||||
| Culture growth | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | enzymeActivity | ||||||||
| Compound (if concentration) | Sso PGK-GAPDH activity at 70C | ||||||||
| Unit | ug/ml | ||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | Glyceraldehyde 3-phosphate | |||||||
| EC Number | 2.7.2.3 | 1.2.1.12 | |||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | KEGG | CHEBI | |||||||
| 3PG | C00197 | 17794 | |||||||
| GAP | C00661 | 17138 | |||||||
| BPG | C00236 | 28907 | |||||||
| PYR | C00022 | 32816 | |||||||
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| time [min] | 3PG | stdev | PYR | stdev | BPG | stdev | GAP | stdev | |
| 0 | 2.969047619047619 | 0.1986633337619347 | 0.3416666666666667 | 0.0061731544389301545 | 0.032857142857142856 | 0.0031426968052735427 | 0.08313492063492064 | 0.02609560740093212 | |
| 20 | 2.375396825396826 | 0.021325442607213575 | 0.9714285714285715 | 0.024692617755720698 | 0.03777777777777778 | 0.016611397399303 | 0.18015873015873018 | 0.08025100770609227 | |
| 40 | 1.8285714285714287 | 0.08305698699651512 | 1.7694444444444448 | 0.023009030181467098 | 0.03420634920634921 | 0.016723636570919916 | 0.16309523809523813 | 0.004489566864676496 | |
| 60 | 1.3246031746031748 | 0.030304576336566216 | 2.4380952380952383 | 0.05050762722761004 | 0.04 | 0.011672873848158877 | 0.1876984126984127 | 0.05219121480186424 | |
| 80 | 0.8373015873015873 | 0.028059792904228133 | 3.043650793650794 | 0.021325442607213262 | 0.024444444444444442 | 0 | 0.15376984126984128 | 0.05359420444707566 | |
| 100 | 0.5047619047619047 | 0.029182184620397175 | 3.5325396825396824 | 0.04377327693059596 | 0.0338095238095238 | 0.015713484026367734 | 0.12202380952380953 | 0.09063313108065665 | |
| 120 | 0.15396825396825398 | 0.017958267458705983 | 3.6690476190476193 | 0.23009030181467002 | 0.009682539682539681 | 0.0035916534917411944 | 0.09742063492063494 | 0.026095607400932055 | |
| 150 | 0.07063492063492063 | 0.023570226039551587 | 3.6793650793650796 | 0.19754094204576558 | 0.0054761904761904765 | 0.007744502841566949 | 0.05972222222222223 | 0.02721799911710121 | |
