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| Metadata | Values (examples) | ||||||||
| Asset Title | PGK reaction 70C | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | |||||||||
| Assay Title | Sso PGK reaction with ATP recycling | ||||||||
| Assay_type | Metabolite concentration | ||||||||
| Technology_type | progressive curve experiment | ||||||||
| Description | Progress curve of Sulfolobus solfataricus Phosphoglycerate kinase. The conversion of metabolites was followed using 13P-NMR | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | concentration | concentration | concentration | concentration | |||||
| Compound (if concentration) | 3PG | Pi | PEP | ATP | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | 5 | 2 | 4 | 10 | |||||
| End_value (optional) | 5 | 5 | 1 | 10 | |||||
| Comments | |||||||||
| Culture growth | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | enzymeActivity | ||||||||
| Compound (if concentration) | Sso PGK activity without ATP recycling system at 70C | ||||||||
| Unit | ug/ml | ||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | ||||||||
| EC Number | 2.7.2.3 | ||||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | KEGG | CHEBI | |||||||
| 3PG | C00197 | 17794 | |||||||
| PEP | C00074 | 44897 | |||||||
| ATP | C00002 | 15422 | |||||||
| Pi | 24838 | ||||||||
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| time | 3PG | pi | atp | pep | |||||
| 7.467 | 6.303 | 1.684 | 10 | 4.113 | |||||
| 8.533 | 5.837 | 1.207 | 9.999 | 4.426 | |||||
| 9.6 | 6.066 | 2.311 | 10.057 | 4.29 | |||||
| 10.666 | 6.148 | 1.42 | 10.045 | 4.808 | |||||
| 11.733 | 6.534 | 2.382 | 10.021 | 3.892 | |||||
| 12.8 | 6.884 | 2.056 | 10.188 | 3.605 | |||||
| 13.866 | 6.987 | 2.253 | 9.967 | 3.573 | |||||
| 14.933 | 6.147 | 2.154 | 10.289 | 3.311 | |||||
| 16 | 6.359 | 2.988 | 9.963 | 2.768 | |||||
| 17.066 | 5.713 | 2.45 | 10.392 | 2.789 | |||||
| 18.133 | 6.365 | 3.479 | 10.492 | 3.56 | |||||
| 19.2 | 6.644 | 3.711 | 10.477 | 2.619 | |||||
| 20.266 | 6.506 | 3.351 | 10.144 | 1.699 | |||||
| 21.333 | 6.353 | 4.319 | 10.5 | 2.647 | |||||
| 22.4 | 6.646 | 2.981 | 10.227 | 2.127 | |||||
| 23.466 | 6.137 | 3.997 | 10.462 | 2.18 | |||||
| 24.533 | 6.362 | 4.637 | 10.219 | 1.259 | |||||
| 25.599 | 6.321 | 4.417 | 10.295 | 1.653 | |||||
| 26.666 | 6.664 | 4.454 | 10.316 | 1.968 | |||||
| 27.733 | 6.762 | 4.884 | 10.438 | 0.997 | |||||
| 28.799 | 6.608 | 4.741 | 10.762 | 1.452 | |||||
| 29.866 | 6.687 | 5.269 | 10.242 | 1.568 | |||||
| 30.933 | 6.521 | 5.011 | 9.891 | 1.426 | |||||
| 31.999 | 6.331 | 5.238 | 10.275 | 1.067 | |||||
| 33.066 | 7.17 | 5.221 | 9.902 | 1.521 | |||||
| 34.133 | 7.086 | 5.585 | 10.015 | 2.029 | |||||
| 35.199 | 6.589 | 5.501 | 10.01 | 1.859 | |||||
| 36.266 | 6.686 | 5.315 | 9.564 | 1.438 | |||||
| 37.333 | 6.978 | 5.134 | 9.756 | 0.567 | |||||
| 38.399 | 6.701 | 5.311 | 9.979 | 1.091 | |||||
| 39.466 | 6.605 | 5.345 | 10.097 | 0.884 | |||||
| 40.532 | 6.802 | 5.541 | 9.724 | 1.228 | |||||
| 41.599 | 7.04 | 5.898 | 9.655 | 1.89 | |||||
| 42.666 | 6.977 | 6.056 | 9.829 | 1.137 | |||||
| 43.732 | 6.378 | 5.73 | 9.884 | 1.21 | |||||
| 44.799 | 6.746 | 5.348 | 9.642 | 1.587 |
