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| Metadata | Values (examples) | ||||||||
| Asset Title | PGK reaction 70C | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | |||||||||
| Assay Title | Sso PGK reaction NO Recycling | ||||||||
| Assay_type | Metabolite concentration | ||||||||
| Technology_type | progressive curve experiment | ||||||||
| Description | Progress curve of Sulfolobus solfataricus Phosphoglycerate kinase. The conversion of metabolites was followed using 13P-NMR | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | concentration | concentration | concentration | concentration | |||||
| Compound (if concentration) | 3PG | Pi | ADP | ATP | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | 5 | 0 | 0 | 9 | |||||
| End_value (optional) | 5 | 1 | 1 | 8 | |||||
| Comments | |||||||||
| Culture growth | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | enzymeActivity | ||||||||
| Compound (if concentration) | Sso PGK activity without ATP recycling system at 70C | ||||||||
| Unit | ug/ml | ||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | ||||||||
| EC Number | 2.7.2.3 | ||||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | KEGG | CHEBI | |||||||
| 3PG | C00197 | 17794 | |||||||
| ADP | C00008 | 16761 | |||||||
| ATP | C00002 | 15422 | |||||||
| Pi | 24838 | ||||||||
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| time | 3PG | pi | atp | adp | |||||
| (min) | (mM) | (mM) | (mM) | (mM) | |||||
| 8.533 | 4.842 | 0.346 | 9.62 | 0.38 | |||||
| 9.6 | 5.235 | 0.642 | 9.536 | 0.599 | |||||
| 10.666 | 4.836 | 0.714 | 9.844 | 0.548 | |||||
| 11.733 | 4.813 | 0.738 | 9.592 | 0.658 | |||||
| 12.8 | 4.839 | 0.696 | 9.608 | 0.889 | |||||
| 13.866 | 4.329 | 0.648 | 9.511 | 0.602 | |||||
| 14.933 | 4.77 | 0.487 | 9.502 | 0.444 | |||||
| 16 | 4.801 | 0.772 | 9.46 | 0.431 | |||||
| 17.066 | 4.795 | 0.775 | 9.377 | 0.6 | |||||
| 18.133 | 4.647 | 0.454 | 9.383 | 0.453 | |||||
| 19.2 | 4.631 | 0.488 | 9.47 | 0.583 | |||||
| 20.266 | 4.724 | 0.908 | 9.459 | 0.685 | |||||
| 21.333 | 4.691 | 0.589 | 9.611 | 0.808 | |||||
| 22.4 | 5.055 | 0.685 | 9.288 | 0.363 | |||||
| 23.466 | 5.276 | 0.818 | 9.334 | 0.724 | |||||
| 24.533 | 4.865 | 0.925 | 9.447 | 0.95 | |||||
| 25.599 | 4.942 | 0.668 | 9.259 | 0.906 | |||||
| 26.666 | 5.091 | 0.805 | 9.414 | 0.714 | |||||
| 27.733 | 4.969 | 0.927 | 9.418 | 1.137 | |||||
| 28.799 | 4.604 | 1.172 | 9.345 | 1.198 | |||||
| 29.866 | 4.893 | 0.901 | 9.358 | 0.989 | |||||
| 30.933 | 4.873 | 0.805 | 9.327 | 1.077 | |||||
| 31.999 | 4.56 | 0.804 | 9.172 | 1.048 | |||||
| 33.066 | 4.439 | 1.115 | 9.237 | 1.208 | |||||
| 34.133 | 4.345 | 0.828 | 9.411 | 1.22 | |||||
| 35.199 | 4.718 | 1.386 | 9.392 | 0.873 | |||||
| 36.266 | 4.628 | 0.781 | 9.072 | 1.152 | |||||
| 37.333 | 4.981 | 1.29 | 9.022 | 1.16 | |||||
| 38.399 | 4.93 | 1.099 | 9.107 | 1.164 | |||||
| 39.466 | 4.719 | 0.888 | 9.357 | 0.774 | |||||
| 40.532 | 4.844 | 0.737 | 8.859 | 1.116 | |||||
| 41.599 | 4.201 | 1.142 | 9.075 | 0.99 | |||||
| 42.666 | 4.781 | 0.949 | 9.048 | 0.965 | |||||
| 43.732 | 4.853 | 1.261 | 8.854 | 1.04 | |||||
| 44.799 | 4.946 | 1.064 | 9.069 | 1.122 | |||||
| 45.866 | 4.991 | 0.991 | 9.117 | 1.387 | |||||
| 46.932 | 4.924 | 0.964 | 8.869 | 1.092 | |||||
| 47.999 | 4.416 | 0.902 | 8.741 | 1.296 | |||||
| 49.066 | 4.676 | 1.217 | 8.964 | 1.77 | |||||
| 50.132 | 5.102 | 1.052 | 8.882 | 1.182 | |||||
| 51.199 | 4.74 | 1.103 | 8.963 | 1.118 | |||||
| 52.266 | 4.638 | 1.011 | 8.82 | 1.353 | |||||
| 53.332 | 4.824 | 1.111 | 8.808 | 1.165 |
