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| Metadata | Values (examples) | ||||||||
| Asset Title | yeast PGK reaction | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | |||||||||
| Assay Title | yeast PGK reaction at 30C NoRecycling | ||||||||
| Assay_type | Metabolite concentration | ||||||||
| Technology_type | progressive curve experiment | ||||||||
| Description | Progress curve of yeast Phosphoglycerate kinase. Measures 3PG consumption and BPG formation | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | concentration | concentration | |||||||
| Compound (if concentration) | 3PG | BPG | |||||||
| Unit | mM | mM | |||||||
| Start_value (optional) | 4 | 0 | |||||||
| End_value (optional) | 4 | 0 | |||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | enzymeActivity | ||||||||
| Compound (if concentration) | S. cereviseae PGK activity No ATP recycling | ||||||||
| Unit | ug/ml | ||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | ||||||||
| EC Number | 2.7.2.3 | ||||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | KEGG | CHEBI | |||||||
| BPG | C00236 | 16001 | |||||||
| 3PG | C00197 | 17794 | |||||||
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| time | time | mM BPG | stdev | mM 3PG | stdev | ||||
| 0 | 0 | 0.12456140350877191 | 0.04558028440970731 | 3.6982456140350877 | 0.2670415186230662 | ||||
| 5 | 5 | 0.2631578947368421 | 0.25922400792164074 | 3.3257309941520474 | 0.3997164789801361 | ||||
| 10 | 10 | 0.32982456140350874 | 0.15750764767015923 | 3.337426900584795 | 0.4724165406865363 | ||||
| 20 | 20 | 0.2245614035087719 | 0.2639112025038299 | 3.1228070175438596 | 0.38287438904154136 | ||||
| 30 | 30 | 0.29298245614035084 | 0.18847201114304182 | 3.0538011695906433 | 0.4601923043819992 | ||||
