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| Metadata | Values (examples) | ||||||||
| Asset Title | yeast PGK reaction | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | |||||||||
| Assay Title | yeast PGK reaction at 30C including ATP recycling | ||||||||
| Assay_type | Metabolite concentration | ||||||||
| Technology_type | progressive curve experiment | ||||||||
| Description | Progress curve of yeast Phosphoglycerate kinase. Measures 3PG consumption and BPG formation in presence of an ATP recycling system | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | concentration | concentration | concentration | ||||||
| Compound (if concentration) | 3PG | ATP | BPG | PYR | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | 4 | 10 | 0 | 0 | |||||
| End_value (optional) | 2 | 10 | 2 | 2 | |||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | enzymeActivity | ||||||||
| Compound (if concentration) | S. cereviseae PGK activity in presence of ATP recycling system | ||||||||
| Unit | ug/ml | ||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | ||||||||
| EC Number | 2.7.2.3 | ||||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | KEGG | CHEBI | |||||||
| BPG | C00236 | 16001 | |||||||
| 3PG | C00197 | 17794 | |||||||
| PYR | C00022 | 32816 | |||||||
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| time | mM BPG | stdev | mM 3PG | stdev | time | mM PYR | |||
| 0 | 0.1131578947368421 | 0.06822960169343882 | 4.016666666666667 | 0.14762404730035 | 0 | 0.2175438596491228 | |||
| 5 | 0.8947368421052632 | 0.16126996763903673 | 3.419298245614035 | 0.05458368135475121 | 5 | 0.7175438596491228 | |||
| 10 | 1.2999999999999998 | 0.004962152850432085 | 2.7359649122807017 | 0.006202691063040067 | 10 | 1.1614035087719297 | |||
| 20 | 1.6096491228070176 | 0.12529435947340586 | 2.3 | 0.027291840677375605 | 20 | 1.4912280701754388 | |||
| 30 | 1.776315789473684 | 0.10792682449689411 | 2.0596491228070173 | 0.06698906348083071 | 30 | 1.7578947368421052 | |||
