Effect of growth rate and pH on pyruvate branching when L-LDH is knocked out

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Metadata Environmental parameters Measurements environ parameters Medium composition (calculated) Extracellular metabolites Calculated fluxes

Item	Value
Experimentator	Tomas Fiedler
Raw data
Reference	SYSMO-LAB

Description	Growth in glucose-limited chemostat conditions at different growth rates and pH
Aim	Effect of growth rate and pH on pyruvate branching when L-LDH is knocked out
Strain information	M49 (591) Δldh
Strain	Streptococcus pyogenes
Vessel type	Sartorius fermentor (Biostat Bplus)
Stirring rate	100	RPM
Temperature	37	degree celcius
Dilution rate	Variable	/h
Volume	750	ml
Gas flow	50	ml
pH
SOP's used
Jermtype	1

	experiment run	measurementnumber	Age	Date	Chemostat	Medium flow	Volume	Temperature	pH Chemostat	Dilution rate	%O2 input	Airflow	N2 flow	Total flow	Comments
Unit	1	1	hours	Date	name	ml/hour	ml	Celcius	1	1/hour	volume%	ml/min	ml/min	ml/min	1
	1	1				37.5	750	37	6.5	0.05	0	0	50	50
	2	1				37.5	750	37	7.5	0.05	0	0	50	50
	3	1				112.5	750	37	6.5	0.15	0	0	50	50
	4	1				112.5	750	37	7.5	0.15	0	0	50	50


















						37.5

Identifier

experiment run

measurementnumber

CO2

RPM

standard deviation OD

Dry weight

standard deviation dryweight

Unit

volume%

rpm

gram/liter

Description

Inputgas

Outputgas

stirring rate

Optical density (600nm)

Dryweight measured as described in SOP X

6.45

100

2.27

7.45

100

1.65

0.65

6.45

100

2.94

1.25

7.45

100

2.43

1.05

Identifier	Experiment	measurementnumber	Glucose	Ethanol	Acetate	Lactate	Formiate	Succinate	Pyruvate	Acetoin	2,3 Butanediol	%O2	CO2	Aspartic acid	Serine	Glutamate	Glycine	Histidine	NH3	Arginine	Threonine	Alanine	Proline	Cysteine	Tyrosine	Valine	Methionine	Ornithine	Lysine	Isoluecine	Leucine	Phenylalanine
Unit			mM	mM	mM	mM	mM	mM	mM	mM	mM	%	%	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	μM	mM	mM	mM	mM
	1	1	61.05	0	12.2	0	0	0	0	0	0			3.16	3.24	3.4	2.33	0.97		0.72	1.89	2.7	5.86	0.83	1.38	2.77	0.84	0	2.41	1.6	3.6	1.66
	2	1	61.05	0	12.2	0	0	0	0	0	0			3.16	3.24	3.4	2.33	0.97		0.72	1.89	2.7	5.86	0.83	1.38	2.77	0.84	0	2.41	1.6	3.6	1.66
	3	1	61.05	0	12.2	0	0	0	0	0	0			3.16	3.24	3.4	2.33	0.97		0.72	1.89	2.7	5.86	0.83	1.38	2.77	0.84	0	2.41	1.6	3.6	1.66
	4	1	61.05	0	12.2	0	0	0	0	0	0			3.16	3.24	3.4	2.33	0.97		0.72	1.89	2.7	5.86	0.83	1.38	2.77	0.84	0	2.41	1.6	3.6	1.66

Identifier	Experiment	measurementnumber	Glucose	Ethanol	Acetate	Lactate	Formiate	Succinate	Pyruvate	Acetoin	2,3 Butanediol	CO2	O2	Aspartic acid	Serine	Glutamate	Glycine	Histidine	NH3	Arginine	Threonine	Alanine	Proline	Cysteine	Tyrosine	Valine	Methionine	Ornithine	Lysine	Isoluecine	Leucine	Phenylalanine
Unit	1	1	mM	mM	mM	mM	mM	mM	mM	mM	mM	%	%	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	μM	mM	mM	mM	mM
	1	1	0	44.14	40.34	1.75	65.44	0	8.2	3.15	0			0.3306	0.011	0.3718	0.2658	0.0505	1.5942	0	0.1775	0.4432	0.7049	0.0015	0.1934	0.2438	0.0958	2.0498	0.224	0.0877	0.2798	0.2009
	2	1	0	61.08	40.8	1.72	71.47	0	15.35	2.6	0			0.3442	0.008	0.387	0.2079	0.0656	2.1152	0	0.2135	0.7143	0.7984	0.0033	0.1463	0.1664	0.0921	0.9542	0.215	0.0418	0.1465	0.2199
	3	1	0	55.65	42.12	2.25	74.6	0	19.53	2.98	0			0.5689	0.031	0.6046	0.4209	0.2097	1.6867	0	0.2923	0.6531	1.1723	0.0028	0.3745	0.394	0.1606	2.7241	0.3278	0.129	0.4032	0.2813
	4	1	0	55.26	39.59	0.8	69	0	15.02	2.51	0			0.1075	0.0068	0.1124	0.0719	0.0226	0.6755	0.0072	0.0604	0.2059	0.2589	0.0041	0.0707	0.0605	0.0336	0.3886	0.0726	0.0186	0.0593	0.0622

Identifier	experiment run	measurementnumber	Glucose	Ethanol	Acetate	Lactate	Formate	Succinate	Pyruvate	Acetoin	2,3-butanediol	CO2	O2	Aspartic acid	Serine	Glutamate	Glycine	Histidine	NH3	Arginine	Threonine	Alanine	Proline	Cysteine	Tyrosine	Valine	Methionine	Ornithine	Lysine	Isoluecine	Leucine	Phenylalanine	Yield glucose	Yield glucose	ATP (calculated)	Lactate (mol/mol glucose)	Formate (mol/mol glucose)
Unit	1	1	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	g/mol	g/g	mmol/gram/hour	mol/mol	mol/mol
	1	1	-3.0525	2.2070000000000003	1.4070000000000003	0.08750000000000001	3.2720000000000002	0	0.41	0.1575	0	0	0	-0.14147	-0.16145	-0.15141000000000002	-0.10321000000000001	-0.045975	0.07971	-0.036	-0.085625	-0.11284000000000001	-0.257755	-0.041425000000000003	-0.059329999999999994	-0.12631	-0.03721	1.0249E-4	-0.10930000000000001	-0.07561500000000002	-0.16601000000000002	-0.07295499999999999	16.38001638001638	0.09100009100009099	5.833500000000002	0.028665028665028666	1.071908271908272
	2	1	-4.696153846153846	4.6984615384615385	2.1999999999999997	0.13230769230769232	5.497692307692308	0	1.1807692307692308	0.2	0	0	0	-0.21660000000000004	-0.24861538461538463	-0.23176923076923078	-0.16323846153846153	-0.06956923076923077	0.16270769230769233	-0.05538461538461538	-0.12896153846153846	-0.15274615384615386	-0.38935384615384616	-0.0635923076923077	-0.09489999999999998	-0.2002769230769231	-0.05753076923076923	7.34E-5	-0.16884615384615387	-0.11986153846153846	-0.2656538461538462	-0.11077692307692308	10.647010647010648	0.05915005915005915	10.811538461538463	0.028173628173628174	1.1706797706797707
	3	1	-7.325999999999999	6.678	3.5904	0.26999999999999996	8.952	0	2.3436	0.35760000000000003	0	0	0	-0.310932	-0.38508	-0.33544799999999997	-0.229092	-0.091236	0.20240399999999997	-0.0864	-0.19172399999999998	-0.245628	-0.562524	-0.09926399999999999	-0.12065999999999996	-0.28512	-0.081528	3.2689199999999996E-4	-0.24986400000000003	-0.17652	-0.383616	-0.16544399999999998	20.475020475020475	0.11375011375011375	17.187599999999996	0.036855036855036855	1.221949221949222
	4	1	-8.72142857142857	7.894285714285713	3.912857142857143	0.11428571428571428	9.857142857142856	0	2.1457142857142855	0.3585714285714285	0	0	0	-0.4360714285714286	-0.46188571428571423	-0.46965714285714283	-0.3225857142857143	-0.13534285714285713	0.09649999999999999	-0.10182857142857142	-0.26137142857142853	-0.35629999999999995	-0.8001571428571429	-0.11798571428571428	-0.18704285714285712	-0.38707142857142857	-0.1152	5.5514285714285704E-5	-0.3339142857142857	-0.2259142857142857	-0.5058142857142858	-0.22825714285714283	17.1990171990172	0.09555009555009555	18.697142857142854	0.013104013104013107	1.1302211302211302