A series of fermentor runs of M49 (591) wild type and ldh knock out mutant strains at different pH (6.5 and 7.5) and dilution rates (0.05 1/h and 0.15 1/h).

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Metadata Environmental parameters Measurements environ parameters Medium composition (calculated) Extracellular metabolites Calculated fluxes

Item	Value
Experimentator	Tomas Fiedler
Raw data
Reference	SYSMO-LAB

Description	Growth in glucose-limited chemostat conditions at different growth rates and pH
Aim	Effect of growth rate and pH on pyruvate branching
Strain information	M49 (591)
Strain	Streptococcus pyogenes
Vessel type	Sartorius fermentor (Biostat Bplus)
Stirring rate	100	RPM
Temperature	37	degree celcius
Dilution rate	Variable	/h
Volume	750	ml
Gas flow	50	ml
pH
SOP's used
Jermtype	1

	experiment run	measurementnumber	Age	Date	Chemostat	Medium flow	Volume	Temperature	pH Chemostat	Dilution rate	%O2 input	Airflow	N2 flow	Total flow	Comments
Unit	1	1	hours	Date	name	ml/hour	ml	Celcius	1	1/hour	volume%	ml/min	ml/min	ml/min	1
	1	1				37.5	750	37	6.5	0.05	0	0	50	50
	2	1				37.5	750	37	7.5	0.05	0	0	50	50
	3	1				112.5	750	37	6.5	0.15	0	0	50	50
	4	1				112.5	750	37	7.5	0.15	0	0	50	50


















						37.5

Identifier	experiment run	measurementnumber	O2	O2	CO2	pH	RPM	OD	standard deviation OD	Dry weight	standard deviation dryweight
Unit	1	1	volume%	volume%	volume%	1	rpm	AU	AU	gram/liter	gram/liter
Description			Inputgas	Outputgas	Outputgas		stirring rate	Optical density (600nm)	Optical density (600nm)	Dryweight measured as described in SOP X
	1	1	?	?	?	6.45	100	2.55		1.2
	2	1	?	?	?	7.45	100	2.22		0.65
	3	1	?	?	?	6.45	100	2.36		0.7
	4	1	?	?	?	7.45	100	2.1		1

Identifier	Experiment	measurementnumber	Glucose	Ethanol	Acetate	Lactate	Formiate	Succinate	Pyruvate	Acetoin	2,3 Butanediol	%O2	CO2	Aspartic acid	Serine	Glutamate	Glycine	Histidine	NH3	Arginine	Threonine	Alanine	Proline	Cysteine	Tyrosine	Valine	Methionine	Ornithine	Lysine	Isoluecine	Leucine	Phenylalanine
Unit			mM	mM	mM	mM	mM	mM	mM	mM	mM	%	%	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	μM	mM	mM	mM	mM
	1	1	61.05	0	12.2	0	0	0	0	0	0			3.16	3.24	3.4	2.33	0.97		0.72	1.89	2.7	5.86	0.83	1.38	2.77	0.84	0	2.41	1.6	3.6	1.66
	2	1	61.05	0	12.2	0	0	0	0	0	0			3.16	3.24	3.4	2.33	0.97		0.72	1.89	2.7	5.86	0.83	1.38	2.77	0.84	0	2.41	1.6	3.6	1.66
	3	1	61.05	0	12.2	0	0	0	0	0	0			3.16	3.24	3.4	2.33	0.97		0.72	1.89	2.7	5.86	0.83	1.38	2.77	0.84	0	2.41	1.6	3.6	1.66
	4	1	61.05	0	12.2	0	0	0	0	0	0			3.16	3.24	3.4	2.33	0.97		0.72	1.89	2.7	5.86	0.83	1.38	2.77	0.84	0	2.41	1.6	3.6	1.66

Identifier	Experiment	measurementnumber	Glucose	Ethanol	Acetate	Lactate	Formiate	Succinate	Pyruvate	Acetoin	2,3 Butanediol	CO2	O2	Aspartic acid	Serine	Glutamate	Glycine	Histidine	NH3	Arginine	Threonine	Alanine	Proline	Cysteine	Tyrosine	Valine	Methionine	Ornithine	Lysine	Isoluecine	Leucine	Phenylalanine
Unit	1	1	mM	mM	mM	mM	mM	mM	mM	mM	mM	%	%	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	mM	μM	mM	mM	mM	mM
	1	1	0	19.26	35.1	69.97	35.41	0	1.5	0	0			0.4196	0.0397	0.5093	0.3461	0.0916	1.7007	0	0.2182	0.3751	0.8403	0.0013	0.2612	0.3379	0.1219	2.5458	0.2546	0.1635	0.3945	0.2532
	2	1	0	8.32	22.84	83.79	13.63	0	0	0	0			0.3494	0.0133	0.4202	0.2785	0.087	1.9354	0.0488	0.1964	0.3841	0.7889	0.0019	0.1557	0.3216	0.0887	1.296	0.2229	0.1592	0.3836	0.2292
	3	1	0	0	20.82	99.67	8.14	0	1.44	0	0			1.0399	0.026	1.1502	0.732	0.1807	5.5765	0	0.5721	0.7386	2.2655	7.0E-4	0.5838	0.8574	0.2398	4.894	0.6218	0.0918	0.9873	0
	4	1	0	11	20.22	56.22	20.69	0	0	0	0			0.2098	0.0062	0.259	0.2474	0.1117	1.235	0.0187	0.173	0.2176	0.6305	0.0019	0.269	0.2253	0.103	0.4674	0.1444	0.104	0.2738	0.265

Identifier	experiment run	measurementnumber	Glucose	Ethanol	Acetate	Lactate	Formate	Succinate	Pyruvate	Acetoin	2,3-butanediol	CO2	O2	Aspartic acid	Serine	Glutamate	Glycine	Histidine	NH3	Arginine	Threonine	Alanine	Proline	Cysteine	Tyrosine	Valine	Methionine	Ornithine	Lysine	Isoluecine	Leucine	Phenylalanine	Yield glucose	Yield glucose	ATP (calculated)	Lactate (mol/mol glucose)	Formate (mol/mol glucose)
Unit	1	1	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	mmol/gram/hour	g/mol	g/g	mmol/gram/hour	mol/mol	mol/mol
	1	1	-2.54375	0.8025000000000001	0.9541666666666669	2.9154166666666668	1.4754166666666666	0	0.06250000000000001	0	0	0	0	-0.11418333333333335	-0.13334583333333336	-0.12044583333333334	-0.08266250000000001	-0.0366	0.07086250000000001	-0.03	-0.06965833333333334	-0.09687083333333336	-0.2091541666666667	-0.034529166666666666	-0.04661666666666666	-0.10133750000000002	-0.029920833333333334	1.0607500000000002E-4	-0.08980833333333335	-0.05985416666666668	-0.13356250000000003	-0.05861666666666667	19.656019656019655	0.1092001092001092	5.688750000000001	1.146109746109746	0.5800163800163799
	2	1	-4.696153846153846	0.64	0.8184615384615385	6.445384615384616	1.0484615384615386	0	0	0	0	0	0	-0.21620000000000003	-0.2482076923076923	-0.22921538461538463	-0.1578076923076923	-0.06792307692307692	0.14887692307692307	-0.05163076923076923	-0.13027692307692307	-0.17814615384615384	-0.3900846153846154	-0.06369999999999999	-0.09417692307692307	-0.18833846153846154	-0.05779230769230769	9.969230769230769E-5	-0.16823846153846153	-0.11083076923076923	-0.24741538461538465	-0.11006153846153845	10.647010647010648	0.05915005915005915	8.722307692307693	1.3724815724815724	0.22325962325962326
	3	1	-13.082142857142856	0	1.8471428571428574	21.357857142857146	1.7442857142857144	0	0.3085714285714286	0	0	0	0	-0.45430714285714285	-0.6887142857142858	-0.48210000000000003	-0.34242857142857147	-0.1691357142857143	1.1949642857142857	-0.1542857142857143	-0.28240714285714286	-0.42030000000000006	-0.7702500000000001	-0.17770714285714284	-0.1706142857142857	-0.4098428571428571	-0.1286142857142857	0.0010487142857142859	-0.38318571428571435	-0.3231857142857143	-0.5598642857142857	-0.3557142857142857	11.466011466011466	0.0637000637000637	25.36071428571429	1.632596232596233	0.13333333333333336
	4	1	-9.157499999999999	1.65	1.2029999999999998	8.433	3.1035	0	0	0	0	0	0	-0.44253000000000003	-0.48507	-0.47114999999999996	-0.31239	-0.128745	0.18525	-0.10519499999999998	-0.25754999999999995	-0.37236	-0.784425	-0.12421499999999999	-0.16664999999999996	-0.381705	-0.11055	7.010999999999999E-5	-0.33984	-0.2244	-0.49893	-0.20925	16.38001638001638	0.09100009100009099	12.488999999999999	0.920884520884521	0.33890253890253896