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iNS142_ko_biomass_fba_results.x
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| Reaction id | Reaction name | Reaction definition | Lower bound | Upper bound | Flux | Subsystem | |||
| PGK | phosphoglycerate kinase | 3pg[c] + atp[c] <=> 13dpg[c] + adp[c] | -1000 | 1000 | -19.787791 | Glycolysis/Gluconeogenesis | |||
| PGM | phosphoglycerate mutase | 2pg[c] <=> 3pg[c] | -1000 | 1000 | -19.668367 | Glycolysis/Gluconeogenesis | |||
| CO2t | CO2 transporter via diffusion | co2[e] <=> co2[c] | -1000 | 1000 | -18.427845 | Transport, Extracellular | |||
| H2Ot | H2O transport via diffusion | h2o[e] <=> h2o[c] | -1000 | 1000 | -10.067156 | Transport, Extracellular | |||
| EX_glc(e) | D-Glucose exchange | glc_D[e] <=> | -10 | 1000 | -10 | Exchange | |||
| ATPS4r | ATP synthase (four protons for one ATP) | adp[c] + 4 h[e] + pi[c] <=> atp[c] + h2o[c] + 3 h[c] | -1000 | 1000 | -5.77962 | Oxidative Phosphorylation | |||
| EX_nh4(e) | Ammonium exchange | nh4[e] <=> | -1000 | 1000 | -0.43529 | Exchange | |||
| GLUDy | glutamate dehydrogenase (NADP) | glu_L[c] + h2o[c] + nadp[c] <=> akg[c] + h[c] + nadph[c] + nh4[c] | -1000 | 1000 | -0.414878 | Glutamate Metabolism | |||
| FUM | fumarase | fum[c] + h2o[c] <=> mal_L[c] | -1000 | 1000 | -0.300779 | Citric Acid Cycle | |||
| MDH | malate dehydrogenase | mal_L[c] + nad[c] <=> h[c] + nadh[c] + oaa[c] | -1000 | 1000 | -0.300779 | Citric Acid Cycle | |||
| EX_pi(e) | Phosphate exchange | pi[e] <=> | -1000 | 1000 | -0.293666 | Exchange | |||
| RPE | ribulose 5-phosphate 3-epimerase | ru5p_D[c] <=> xu5p_D[c] | -1000 | 1000 | -0.057381 | Pentose Phosphate Pathway | |||
| RPI | ribose-5-phosphate isomerase | r5p[c] <=> ru5p_D[c] | -1000 | 1000 | -0.057381 | Pentose Phosphate Pathway | |||
| TKT2 | transketolase | e4p[c] + xu5p_D[c] <=> f6p[c] + g3p[c] | -1000 | 1000 | -0.0431 | Pentose Phosphate Pathway | |||
| TALA | transaldolase | g3p[c] + s7p[c] <=> e4p[c] + f6p[c] | -1000 | 1000 | -0.014281 | Pentose Phosphate Pathway | |||
| TKT1 | transketolase | r5p[c] + xu5p_D[c] <=> g3p[c] + s7p[c] | -1000 | 1000 | -0.014281 | Pentose Phosphate Pathway | |||
| GLNS | glutamine synthetase | atp[c] + glu_L[c] + nh4[c] => adp[c] + gln_L[c] + h[c] + pi[c] | 0 | 1000 | 0.020412 | Glutamate Metabolism | |||
| Biomass_Ecoli_core_w_GAM | Biomass Objective Function with GAM | 1.496 3pg[c] + 3.7478 accoa[c] + 59.81 atp[c] + 0.361 e4p[c] + 0.0709 f6p[c] + 0.129 g3p[c] + 0.205 g6p[c] + 0.2557 gln_L[c] + 4.9414 glu_L[c] + 59.81 h2o[c] + 3.547 nad[c] + 13.0279 nadph[c] + 1.7867 oaa[c] + 0.5191 pep[c] + 2.8328 pyr[c] + 0.8977 r5p[c] => 59.81 adp[c] + 4.1182 akg[c] + 3.7478 coa[c] + 59.81 h[c] + 3.547 nadh[c] + 13.0279 nadp[c] + 59.81 pi[c] | 0 | 1000 | 0.079829 | Exchange | |||
| CS | citrate synthase | accoa[c] + h2o[c] + oaa[c] => cit[c] + coa[c] + h[c] | 0 | 1000 | 0.086127 | Citric Acid Cycle | |||
| ACONTa | aconitase (half-reaction A, Citrate hydro-lyase) | cit[c] <=> acon_C[c] + h2o[c] | -1000 | 1000 | 0.086127 | Citric Acid Cycle | |||
| ACONTb | aconitase (half-reaction B, Isocitrate hydro-lyase) | acon_C[c] + h2o[c] <=> icit[c] | -1000 | 1000 | 0.086127 | Citric Acid Cycle | |||
| ICDHyr | isocitrate dehydrogenase (NADP) | icit[c] + nadp[c] <=> akg[c] + co2[c] + nadph[c] | -1000 | 1000 | 0.086127 | Citric Acid Cycle | |||
| PIt2r | phosphate reversible transport via proton symport | h[e] + pi[e] <=> h[c] + pi[c] | -1000 | 1000 | 0.293666 | Inorganic Ion Transport and Metabolism | |||
| EX_succ(e) | Succinate exchange | succ[e] <=> | 0 | 1000 | 0.300779 | Exchange | |||
| FRD7 | fumarate reductase | fum[c] + q8h2[c] => q8[c] + succ[c] | 0 | 1000 | 0.300779 | Oxidative Phosphorylation | |||
| NADH16 | NADH dehydrogenase (ubiquinone-8 & 3 protons) | 4 h[c] + nadh[c] + q8[c] => 3 h[e] + nad[c] + q8h2[c] | 0 | 1000 | 0.300779 | Oxidative Phosphorylation | |||
| SUCCt3 | succinate transport out via proton antiport | h[e] + succ[c] => h[c] + succ[e] | 0 | 1000 | 0.300779 | Transport, Extracellular | |||
| NH4t | ammonia reversible transport | nh4[e] <=> nh4[c] | -1000 | 1000 | 0.43529 | Inorganic Ion Transport and Metabolism | |||
| PPC | phosphoenolpyruvate carboxylase | co2[c] + h2o[c] + pep[c] => h[c] + oaa[c] + pi[c] | 0 | 1000 | 0.529536 | Anaplerotic reactions | |||
| THD2 | NAD(P) transhydrogenase | 2 h[e] + nadh[c] + nadp[c] => 2 h[c] + nad[c] + nadph[c] | 0 | 1000 | 1.368752 | Oxidative Phosphorylation | |||
| ATPM | ATP maintenance requirement | atp[c] + h2o[c] => adp[c] + h[c] + pi[c] | 8.39 | 1000 | 8.39 | Oxidative Phosphorylation | |||
| PYK | pyruvate kinase | adp[c] + h[c] + pep[c] => atp[c] + pyr[c] | 0 | 1000 | 9.097392 | Glycolysis/Gluconeogenesis | |||
| er_001 | acetoacetyl-CoA thiolase | 2 accoa[c] <=> coa[c] + s_0367[c] | -1000 | 1000 | 9.242972 | Exchange | |||
| er_002 | 3-hydroxyacyl-CoA dehydrogenase | h[c] + nadh[c] + s_0367[c] <=> nad[c] + es_001[c] | -1000 | 1000 | 9.242972 | Exchange | |||
| er_003 | enoyl-CoA hydratase | es_001[c] <=> h2o[c] + es_002[c] | -1000 | 1000 | 9.242972 | Exchange | |||
| er_004 | enoyl-CoA reductase | h[c] + nadh[c] + es_002[c] <=> nad[c] + es_003[c] | -1000 | 1000 | 9.242972 | Exchange | |||
| er_027 | alcohol dehydrogenase (to butanol) | 2 nadh[c] + es_003[c] <=> coa[c] + 2 nad[c] | 0 | 1000 | 9.242972 | Exchange | |||
| PFK | phosphofructokinase | atp[c] + f6p[c] => adp[c] + fdp[c] + h[c] | 0 | 1000 | 9.920594 | Glycolysis/Gluconeogenesis | |||
| FBA | fructose-bisphosphate aldolase | fdp[c] <=> dhap[c] + g3p[c] | -1000 | 1000 | 9.920594 | Glycolysis/Gluconeogenesis | |||
| TPI | triose-phosphate isomerase | dhap[c] <=> g3p[c] | -1000 | 1000 | 9.920594 | Glycolysis/Gluconeogenesis | |||
| PGI | glucose-6-phosphate isomerase | g6p[c] <=> f6p[c] | -1000 | 1000 | 9.983635 | Glycolysis/Gluconeogenesis | |||
| GLCpts | D-glucose transport via PEP:Pyr PTS | glc_D[e] + pep[c] => g6p[c] + pyr[c] | 0 | 1000 | 10 | Transport, Extracellular | |||
| EX_h2o(e) | H2O exchange | h2o[e] <=> | -1000 | 1000 | 10.067156 | Exchange | |||
| EX_co2(e) | CO2 exchange | co2[e] <=> | -1000 | 1000 | 18.427845 | Exchange | |||
| PDH | pyruvate dehydrogenase | coa[c] + nad[c] + pyr[c] => accoa[c] + co2[c] + nadh[c] | 0 | 1000 | 18.871253 | Glycolysis/Gluconeogenesis | |||
| ENO | enolase | 2pg[c] <=> h2o[c] + pep[c] | -1000 | 1000 | 19.668367 | Glycolysis/Gluconeogenesis | |||
| GAPD | glyceraldehyde-3-phosphate dehydrogenase | g3p[c] + nad[c] + pi[c] <=> 13dpg[c] + h[c] + nadh[c] | -1000 | 1000 | 19.787791 | Glycolysis/Gluconeogenesis | |||
| EX_h(e) | H+ exchange | h[e] <=> | -1000 | 1000 | 20.688866 | Exchange |
