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| Metadata | Values (examples) | ||||||||
| Asset Title | TPI Kinetic Data | ||||||||
| Uploader | Dawie van Niekerk | ||||||||
| Uploader SEEK ID | |||||||||
| Project | |||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 0 | ||||||||
| Assay Title | TPI | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of TPI. Experimental data for enzyme reaction rates. | ||||||||
| Experimentalist | Gerald Penkler | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | pH | Buffer | Buffer | Buffer | Buffer | |||
| Compound (if concentration) | HEPES | MgCl | KCl | NaCl | |||||
| Unit | °C | mM | mM | mM | mM | ||||
| Start_value (optional) | 37 | 7.17 | 20 | 20 | 10 | 20 | |||
| End_value (optional) | 37 | ||||||||
| Comments | |||||||||
| Culture growth | Batch | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | concentration | ||||||
| Compound (if concentration) | GAP | DHAP | PEP | ||||||
| Unit | mM | mM | mM | ||||||
| Start_value (optional) | 0 | 0 | 0 | ||||||
| End_value (optional) | 1.2 | 15 | 20 | ||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | triose-phosphate isomerase | ||||||||
| EC Number | 5.3.1.1 | ||||||||
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| Date | DHAP | GAP | PEP | v | ControlRate | v/ControlRate | mean (v/V) | SEM (v/V) | Vf_model | Vr_model | v_unnormalised |
| yyymmdd | (mM) | (mM) | (mM) | (U/mg) | (U/mg) | 1 | 1 | (M/min) | (M/min) | (fmol/28fL/min) | |
| 20110916 | 0 | 0 | 0 | 0 | 2.77716 | 0 | 0 | 0 | 1.506 | 5.71472 | 0 |
| 20110916 | 15.385 | 0 | 0 | 2.98534836179097 | 2.77716 | 1.074964482345623 | 0.9236731848303111 | 0.08622173788880885 | 1.506 | 5.71472 | 45.329102291550235 |
| 20110916 | 15.385 | 0 | 0 | 2.55413137619896 | 2.77716 | 0.9196918348957064 | 1.506 | 5.71472 | 38.781565293882146 | ||
| 20110916 | 15.385 | 0 | 0 | 2.15608492796011 | 2.77716 | 0.776363237249604 | 1.506 | 5.71472 | 32.7376849883413 | ||
| 20110916 | 9.091 | 0 | 0 | 2.25283232857377 | 2.77716 | 0.8112000491774943 | 0.7800128080230117 | 0.015970264969103597 | 1.506 | 5.71472 | 34.206683673716576 |
| 20110916 | 9.091 | 0 | 0 | 2.13949965928353 | 2.77716 | 0.7703912123476969 | 1.506 | 5.71472 | 32.48585664227768 | ||
| 20110916 | 9.091 | 0 | 0 | 2.10632912193026 | 2.77716 | 0.7584471625438434 | 1.506 | 5.71472 | 31.982199950148782 | ||
| 20110916 | 2.5 | 0 | 0 | 1.39159897448245 | 2.77716 | 0.5010870725786235 | 0.5673758787290026 | 0.07940023706503807 | 1.506 | 5.71472 | 21.129839676495397 |
| 20110916 | 2.5 | 0 | 0 | 2.01480300960379 | 2.77716 | 0.725490432529559 | 1.506 | 5.71472 | 30.592480558906445 | ||
| 20110916 | 2.5 | 0 | 0 | 1.32067880202687 | 2.77716 | 0.47555013107882516 | 1.506 | 5.71472 | 20.0529979273319 | ||
| 20110916 | 1.25 | 0 | 0 | 1.1302553468509 | 2.77716 | 0.40698243776048193 | 0.37380452163870365 | 0.049475200692331356 | 1.506 | 5.71472 | 17.161635435484 |
| 20110916 | 1.25 | 0 | 0 | 0.767836512806317 | 2.77716 | 0.2764826343481532 | 1.506 | 5.71472 | 11.658719725192924 | ||
| 20110916 | 1.25 | 0 | 0 | 1.21625303628521 | 2.77716 | 0.437948492807476 | 1.506 | 5.71472 | 18.467412044705647 | ||
| 20110916 | 0.625 | 0 | 0 | 0.824144523745461 | 2.77716 | 0.29675802753368946 | 0.258541983408231 | 0.027742584515187005 | 1.506 | 5.71472 | 12.513692505040618 |
| 20110916 | 0.625 | 0 | 0 | 0.568199019476675 | 2.77716 | 0.20459714941763346 | 1.506 | 5.71472 | 8.627452596642767 | ||
| 20110916 | 0.625 | 0 | 0 | 0.761693820703872 | 2.77716 | 0.27427077327336996 | 1.506 | 5.71472 | 11.565449967391464 | ||
| 20110916 | 0.3125 | 0 | 0 | 0.491415368196035 | 2.77716 | 0.1769488859828152 | 0.1466530918675766 | 0.01667266489391402 | 1.506 | 5.71472 | 7.461580624123351 |
| 20110916 | 0.3125 | 0 | 0 | 0.398716560104514 | 2.77716 | 0.1435698915815128 | 1.506 | 5.71472 | 6.054055188209231 | ||
| 20110916 | 0.3125 | 0 | 0 | 0.331705373532328 | 2.77716 | 0.11944049803840182 | 1.506 | 5.71472 | 5.0365669212833275 | ||
| 20110916 | 0.15625 | 0 | 0 | 0.174946279976656 | 2.77716 | 0.0629946708063835 | 0.08489643242925639 | 0.01115579334646432 | 1.506 | 5.71472 | 2.6563592785635795 |
| 20110916 | 0.15625 | 0 | 0 | 0.255945504268773 | 2.77716 | 0.0921608781160513 | 1.506 | 5.71472 | 3.886239908397651 | ||
| 20110916 | 0.15625 | 0 | 0 | 0.276421144610272 | 2.77716 | 0.09953374836533438 | 1.506 | 5.71472 | 4.19713910106942 | ||
| 20110916 | 0.078125 | 0 | 0 | 0.103248214093561 | 2.77716 | 0.03717762537756593 | 0.05091601301256733 | 0.013738387635001419 | 1.506 | 5.71472 | 1.5677061069212002 |
| 20110916 | 0.078125 | 0 | 0 | 0.179555615302402 | 2.77716 | 0.06465440064756874 | 1.506 | 5.71472 | 2.7263467665066785 | ||
| 20110807 | 0 | 0 | 0 | 0 | 16.4978 | 0 | 0 | 0 | 1.506 | 5.71472 | 0 |
| 20110807 | 0 | 1.25 | 0 | -12.61627828 | 16.4978 | -0.7647248893791898 | -0.7611909885130723 | 0.01586167758163746 | 1.506 | 5.71472 | -122.36528135532521 |
| 20110807 | 0 | 1.25 | 0 | -12.41113554 | 16.4978 | -0.7522903381056868 | 1.506 | 5.71472 | -120.37560194742126 | ||
| 20110807 | 0 | 1.25 | 0 | -13.23170649 | 16.4978 | -0.8020285425935579 | 1.506 | 5.71472 | -128.3343194820472 | ||
| 20110808 | 0 | 1.25 | 0 | -9.717799129 | 14.0887 | -0.689758397 | 1.506 | 5.71472 | -110.369731 | ||
| 20110808 | 0 | 1.25 | 0 | -10.91520253 | 14.0887 | -0.774748737 | 1.506 | 5.71472 | -123.9692189 | ||
| 20110808 | 0 | 1.25 | 0 | -11.03983525 | 14.0887 | -0.783595027 | 1.506 | 5.71472 | -125.3847328 | ||
| 20110807 | 0 | 0.625 | 0 | -10.33919391 | 16.4978 | -0.6267013729103275 | -0.6927935346021187 | 0.025209685342955142 | 1.506 | 5.71472 | -100.27984035434697 |
| 20110807 | 0 | 0.625 | 0 | -10.74947938 | 16.4978 | -0.6515704748511922 | 1.506 | 5.71472 | -104.25919907316495 | ||
| 20110807 | 0 | 0.625 | 0 | -10.74947938 | 16.4978 | -0.6515704748511922 | 1.506 | 5.71472 | -104.25919907316495 | ||
| 20110808 | 0 | 0.625 | 0 | -10.02354188 | 14.0887 | -0.711459672 | 1.506 | 5.71472 | -113.8421988 | ||
| 20110808 | 0 | 0.625 | 0 | -10.17560573 | 14.0887 | -0.722252992 | 1.506 | 5.71472 | -115.5692613 | ||
| 20110808 | 0 | 0.625 | 0 | -11.17524449 | 14.0887 | -0.793206221 | 1.506 | 5.71472 | -126.9226408 | ||
| 20110807 | 0 | 0.3125 | 0 | -7.056910127 | 16.4978 | -0.4277485559892834 | -0.48017984766122757 | 0.01873560723983518 | 1.506 | 5.71472 | -68.44497038072618 |
| 20110807 | 0 | 0.3125 | 0 | -7.4671956 | 16.4978 | -0.4526176581119907 | 1.506 | 5.71472 | -72.42432912864115 | ||
| 20110807 | 0 | 0.3125 | 0 | -7.624471697 | 16.4978 | -0.4621508138660912 | 1.506 | 5.71472 | -73.9497499724712 | ||
| 20110808 | 0 | 0.3125 | 0 | -6.690809244 | 14.0887 | -0.474906077 | 1.506 | 5.71472 | -75.99074714 | ||
| 20110808 | 0 | 0.3125 | 0 | -7.128897944 | 14.0887 | -0.506001118 | 1.506 | 5.71472 | -80.96633178 | ||
| 20110808 | 0 | 0.3125 | 0 | -7.856632067 | 14.0887 | -0.557654863 | 1.506 | 5.71472 | -89.23155914 | ||
| 20110807 | 0 | 0.15625 | 0 | -7.056910127 | 16.4978 | -0.4277485559892834 | -0.3075449422570423 | 0.024935822371983375 | 1.506 | 5.71472 | -68.44497038072618 |
| 20110807 | 0 | 0.15625 | 0 | -4.307997461 | 16.4978 | -0.26112557195504854 | 1.506 | 5.71472 | -41.78326679976274 | ||
| 20110807 | 0 | 0.15625 | 0 | -4.431083103 | 16.4978 | -0.2685863025979221 | 1.506 | 5.71472 | -42.977074425107126 | ||
| 20110808 | 0 | 0.15625 | 0 | -4.042363918 | 14.0887 | -0.286922421 | 1.506 | 5.71472 | -45.9110764 | ||
| 20110808 | 0 | 0.15625 | 0 | -4.257787701 | 14.0887 | -0.302212958 | 1.506 | 5.71472 | -48.35774818 | ||
| 20110808 | 0 | 0.15625 | 0 | -4.207926186 | 14.0887 | -0.298673844 | 1.506 | 5.71472 | -47.79144691 | ||
| 20110807 | 0 | 0.078125 | 0 | -2.789941213 | 16.4978 | -0.16910989422832134 | -0.16710552852901395 | 0.006375823855446724 | 1.506 | 5.71472 | -27.059639452845232 |
| 20110807 | 0 | 0.078125 | 0 | -2.461712835 | 16.4978 | -0.1492146125544012 | 1.506 | 5.71472 | -23.876152458392852 | ||
| 20110807 | 0 | 0.078125 | 0 | -2.625827024 | 16.4978 | -0.15916225339136125 | 1.506 | 5.71472 | -25.46789595561904 | ||
| 20110808 | 0 | 0.078125 | 0 | -2.190443502 | 14.0887 | -0.155475204 | 1.506 | 5.71472 | -24.87792317 | ||
| 20110808 | 0 | 0.078125 | 0 | -2.521725454 | 14.0887 | -0.178989222 | 1.506 | 5.71472 | -28.64045205 | ||
| 20110808 | 0 | 0.078125 | 0 | -2.686461287 | 14.0887 | -0.190681985 | 1.506 | 5.71472 | -30.51143635 | ||
| 20110807 | 0 | 0.039625 | 0 | -1.805256079 | 16.4978 | -0.10942404920656087 | -0.11157008658620947 | 0.00864860736103823 | 1.506 | 5.71472 | -17.50917846948809 |
| 20110807 | 0 | 0.039625 | 0 | -2.051427363 | 16.4978 | -0.12434551049230806 | 1.506 | 5.71472 | -19.896793720176877 | ||
| 20110807 | 0 | 0.039625 | 0 | -1.723198985 | 16.4978 | -0.10445022881838789 | 1.506 | 5.71472 | -16.713306725724493 | ||
| 20110808 | 0 | 0.039625 | 0 | -1.37400547 | 14.0887 | -0.097525355 | 1.506 | 5.71472 | -15.60524272 | ||
| 20110808 | 0 | 0.039625 | 0 | -1.226648361 | 14.0887 | -0.087066114 | 1.506 | 5.71472 | -13.93163698 | ||
| 20110808 | 0 | 0.039625 | 0 | -2.065533914 | 14.0887 | -0.146609262 | 1.506 | 5.71472 | -23.45926474 | ||
| 0 | 10 | 20 | 0 | 0.713211 | 0 | -0.05840677515870464 | 0.031357171485752074 | 1.506 | 5.71472 | 0 | |
| 0 | 10 | 20 | 0 | 0.713211 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 20 | 0 | 0.713211 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 20 | -0.0187334210837734 | 0.152488 | -0.12285177249208724 | 1.506 | 5.71472 | -19.657777476287464 | |||
| 0 | 10 | 20 | -0.0063148736235058 | 0.152488 | -0.041412266037365565 | 1.506 | 5.71472 | -6.626466139133505 | |||
| 0 | 10 | 20 | -0.00528844265995021 | 0.201681 | -0.026221818911797392 | 1.506 | 5.71472 | -4.19580988320555 | |||
| 0 | 10 | 20 | -0.0440393795308702 | 0.201681 | -0.21836156866968232 | 1.506 | 5.71472 | -34.94050626382419 | |||
| 0 | 10 | 10 | 0 | 0.713211 | 0 | -0.05460521631521425 | 0.03750643023867731 | 1.506 | 5.71472 | 0 | |
| 0 | 10 | 10 | 0 | 0.713211 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 10 | 0 | 0.152488 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 10 | -0.0381014975267013 | 0.152488 | -0.24986554697222926 | 1.506 | 5.71472 | -39.98152588060786 | |||
| 0 | 10 | 10 | -0.0266967090427749 | 0.201681 | -0.13237096723427047 | 1.506 | 5.71472 | -21.180964388444846 | |||
| 0 | 10 | 10 | 0 | 0.201681 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 10 | 0 | 0.152488 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 5 | 0 | 0.713211 | 0 | -0.013713087591402263 | 0.013713087591402263 | 1.506 | 5.71472 | 0 | |
| 0 | 10 | 5 | -0.0684622743990612 | 0.713211 | -0.09599161313981584 | 1.506 | 5.71472 | -15.359825360386314 | |||
| 0 | 10 | 5 | 0 | 0.152488 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 5 | 0 | 0.152488 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 5 | 0 | 0.201681 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 5 | 0 | 0.201681 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 5 | 0 | 0.152488 | 0 | 1.506 | 5.71472 | 0 | |||
| 0 | 10 | 2.5 | -0.0557021990585923 | 0.713211 | -0.0781005888279798 | -0.2089113437080468 | 0.050558808572705824 | 1.506 | 5.71472 | -12.497043915636914 | |
| 0 | 10 | 2.5 | -0.0721284542265175 | 0.713211 | -0.10113199912300495 | 1.506 | 5.71472 | -16.182349624790128 | |||
| 0 | 10 | 2.5 | -0.0937758733090372 | 0.713211 | -0.13148405353960776 | 1.506 | 5.71472 | -21.039047412428282 | |||
| 0 | 10 | 2.5 | -0.0284992992225951 | 0.152488 | -0.1868953571598755 | 1.506 | 5.71472 | -29.905529793123144 | |||
| 0 | 10 | 2.5 | -0.0557218957343553 | 0.152488 | -0.3654182344470076 | 1.506 | 5.71472 | -58.4713609972521 | |||
| 0 | 10 | 2.5 | -0.0352558909883101 | 0.201681 | -0.17481017541716917 | 1.506 | 5.71472 | -27.97175375848014 | |||
| 0 | 10 | 2.5 | -0.085621449543036 | 0.201681 | -0.4245389974416826 | 1.506 | 5.71472 | -67.93140198487811 | |||
| 0 | 10 | 1.25 | -0.050660093984867 | 0.152488 | -0.33222347978114336 | -0.20274050251420825 | 0.037249898588049085 | 1.506 | 5.71472 | -53.159796602497075 | |
| 0 | 10 | 1.25 | -0.022734812243337 | 0.152488 | -0.1490924678882076 | 1.506 | 5.71472 | -23.856607826522737 | |||
| 0 | 10 | 1.25 | -0.0333258697972019 | 0.152488 | -0.2185474909317579 | 1.506 | 5.71472 | -34.970256086571 | |||
| 0 | 10 | 1.25 | -0.0505612289453838 | 0.713211 | -0.07089238520631874 | 1.506 | 5.71472 | -11.343643684415108 | |||
| 0 | 10 | 1.25 | -0.105557653412293 | 0.713211 | -0.14800340069389423 | 1.506 | 5.71472 | -23.682343832375512 | |||
| 0 | 10 | 1.25 | -0.0638814621824711 | 0.713211 | -0.0895688122904317 | 1.506 | 5.71472 | -14.332099123226524 | |||
| 0 | 10 | 1.25 | -0.0541093853626018 | 0.201681 | -0.26829193311517596 | 1.506 | 5.71472 | -42.929971728334834 | |||
| 0 | 10 | 1.25 | -0.0696412661497448 | 0.201681 | -0.3453040502067363 | 1.506 | 5.71472 | -55.252846930328325 | |||
| 0 | 10 | 0.625 | -0.150403816128505 | 0.152488 | -0.9863321450114435 | -0.42866950475438664 | 0.10733247891408358 | 1.506 | 5.71472 | -157.8251370007143 | |
| 0 | 10 | 0.625 | -0.0307189355204333 | 0.152488 | -0.20145149467783233 | 1.506 | 5.71472 | -32.234688798628454 | |||
| 0 | 10 | 0.625 | -0.127290111467759 | 0.152488 | -0.8347549411610028 | 1.506 | 5.71472 | -133.57094120584495 | |||
| 0 | 10 | 0.625 | -0.0833563318302563 | 0.713211 | -0.11687471425743054 | 1.506 | 5.71472 | -18.701375477714258 | |||
| 0 | 10 | 0.625 | -0.0680704105223057 | 0.713211 | -0.0954421770307885 | 1.506 | 5.71472 | -15.271908901798852 | |||
| 0 | 10 | 0.625 | -0.103340055651527 | 0.713211 | -0.14489408555326122 | 1.506 | 5.71472 | -23.184815600602125 | |||
| 0 | 10 | 0.625 | -0.0951349439367045 | 0.201681 | -0.47170999715741446 | 1.506 | 5.71472 | -75.47933553875174 | |||
| 0 | 10 | 0.625 | -0.0996651793622638 | 0.201681 | -0.4941723779744438 | 1.506 | 5.71472 | -79.07358961202718 | |||
| 0 | 10 | 0.625 | -0.103340055651525 | 0.201681 | -0.5123936099658619 | 1.506 | 5.71472 | -81.98920830083509 | |||
| 0 | 10 | 0.3125 | -0.424448211409913 | 0.713211 | -0.5951229179161749 | -0.6372566787322614 | 0.09196531184130446 | 1.506 | 5.71472 | -95.22690356126984 | |
| 0 | 10 | 0.3125 | -0.328267828528847 | 0.713211 | -0.4602674783883689 | 1.506 | 5.71472 | -73.64839339467623 | |||
| 0 | 10 | 0.3125 | -0.345058211574622 | 0.713211 | -0.4838094358816984 | 1.506 | 5.71472 | -77.41539286381206 | |||
| 0 | 10 | 0.3125 | -0.14015217848018 | 0.152488 | -0.9191030014176852 | 1.506 | 5.71472 | -147.06765651932687 | |||
| 0 | 10 | 0.3125 | -0.0365713550717694 | 0.152488 | -0.23983103635544697 | 1.506 | 5.71472 | -38.3758821622736 | |||
| 0 | 10 | 0.3125 | -0.158336480118432 | 0.152488 | -1.038353707297833 | 1.506 | 5.71472 | -166.14921954873404 | |||
| 0 | 10 | 0.3125 | -0.154724963765189 | 0.201681 | -0.7671766986735934 | 1.506 | 5.71472 | -122.75760065643081 | |||
| 0 | 10 | 0.3125 | -0.11987699895321 | 0.201681 | -0.5943891539272911 | 1.506 | 5.71472 | -95.10949240047833 | |||
| 0 | 10 | 0 | -0.199105950777743 | 0.201681 | -0.9872320683541981 | -0.9473907680649345 | 0.05365928787150504 | 1.506 | 5.71472 | -157.96913567862288 | |
| 0 | 10 | 0 | -0.189572928577165 | 0.201681 | -0.9399642434198809 | 1.506 | 5.71472 | -150.40570891238093 | |||
| 0 | 10 | 0 | -0.75156605709996 | 0.713211 | -1.053777994310183 | 1.506 | 5.71472 | -168.6172930300401 | |||
| 0 | 10 | 0 | -0.568148546307308 | 0.713211 | -0.796606539028854 | 1.506 | 5.71472 | -127.46673298013121 | |||
| 0 | 10 | 0 | -0.728461856429618 | 0.713211 | -1.0213833724236137 | 1.506 | 5.71472 | -163.43375960958687 | |||
| 0 | 10 | 0 | -0.182730055486818 | 0.152488 | -1.1983241664053432 | 1.506 | 5.71472 | -191.7464382467184 | |||
| 0 | 10 | 0 | -0.130331388396807 | 0.152488 | -0.8546993100887085 | 1.506 | 5.71472 | -136.76228275780403 | |||
| 0 | 10 | 0 | -0.11087988803812 | 0.152488 | -0.7271384504886942 | 1.506 | 5.71472 | -116.35099408174901 | |||
