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| Metadata | Values (examples) | ||||||||
| Asset Title | ENO Kinetic Data | ||||||||
| Uploader | Dawie van Niekerk | ||||||||
| Uploader SEEK ID | |||||||||
| Project | |||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 0 | ||||||||
| Assay Title | ENO | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of ENO. Experimental data for enzyme reaction rates. | ||||||||
| Experimentalist | Gerald Penkler | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | pH | Buffer | Buffer | Buffer | Buffer | |||
| Compound (if concentration) | HEPES | MgCl | KCl | NaCl | |||||
| Unit | °C | mM | mM | mM | mM | ||||
| Start_value (optional) | 37 | 7.17 | 20 | 20 | 10 | 20 | |||
| End_value (optional) | 37 | ||||||||
| Comments | |||||||||
| Culture growth | Batch | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | |||||||
| Compound (if concentration) | 2PG | PEP | |||||||
| Unit | mM | mM | |||||||
| Start_value (optional) | 0 | 0 | |||||||
| End_value (optional) | 5 | 12 | |||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | phosphopyruvate hydratase | ||||||||
| EC Number | 4.2.1.11 | ||||||||
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| Date | 2PG | PEP | v | V | v/V | mean (v/V) | SEM (v/V) | Vf_model | Vr_model | v_unnormalised |
| yyyymmdd | (mM) | (mM) | (U/mg) | (U/mg) | 1 | 1 | (M/min) | (M/min) | (fmol/28fL/min) | |
| 20081214 | 0 | 0 | 0 | 1.7667 | 0 | 0 | 0 | 0.286296 | 0.152928 | 0 |
| 20081214 | 10 | 0 | 1.40475465593657 | 1.7667 | 0.795129142433107 | 0.8706841625713156 | 0.1067063694473447 | 0.286296 | 0.152928 | 6.373984202936806 |
| 20081214 | 10 | 0 | 1.29960409674959 | 1.7667 | 0.7356110809699384 | 0.286296 | 0.152928 | 5.896870281046346 | ||
| 20081214 | 10 | 0 | 1.91035437735807 | 1.7667 | 1.0813122643109017 | 0.286296 | 0.152928 | 8.66811052864831 | ||
| 20081214 | 4 | 0 | 1.81829815684415 | 1.7667 | 1.0292059528183337 | 0.9187494866308956 | 0.07830675434971807 | 0.286296 | 0.152928 | 8.250411329106175 |
| 20081214 | 4 | 0 | 1.69545623481722 | 1.7667 | 0.95967410132859 | 0.286296 | 0.152928 | 7.693023982391161 | ||
| 20081214 | 4 | 0 | 1.35570976243104 | 1.7667 | 0.7673684057457633 | 0.286296 | 0.152928 | 6.1514461425588935 | ||
| 20081214 | 3 | 0 | 1.32490460974961 | 1.7667 | 0.7499318558609894 | 0.9104305578313125 | 0.08109389396704887 | 0.286296 | 0.152928 | 6.011669736956179 |
| 20081214 | 3 | 0 | 1.78595405166852 | 1.7667 | 1.0108983141838004 | 0.286296 | 0.152928 | 8.10365202521183 | ||
| 20081214 | 3 | 0 | 1.71451433814361 | 1.7667 | 0.9704615034491482 | 0.286296 | 0.152928 | 7.779498904561365 | ||
| 20081214 | 2 | 0 | 1.69774455173638 | 1.7667 | 0.9609693506177507 | 0.7752489828280279 | 0.0987513681027971 | 0.286296 | 0.152928 | 7.703407073724868 |
| 20081214 | 2 | 0 | 1.10276623832092 | 1.7667 | 0.6241955274358522 | 0.286296 | 0.152928 | 5.0037311162376925 | ||
| 20081214 | 2 | 0 | 1.30838634382953 | 1.7667 | 0.7405820704304806 | 0.286296 | 0.152928 | 5.936719164207016 | ||
| 20081214 | 1.5 | 0 | 1.84876781561018 | 1.7667 | 1.0464526040698365 | 0.7854185089602478 | 0.1398709204840055 | 0.286296 | 0.152928 | 8.388665452573782 |
| 20081214 | 1.5 | 0 | 1.31090528025602 | 1.7667 | 0.7420078566004528 | 0.286296 | 0.152928 | 5.948148676771931 | ||
| 20081214 | 1.5 | 0 | 1.00312354347401 | 1.7667 | 0.5677950662104545 | 0.286296 | 0.152928 | 4.551608775722071 | ||
| 20081214 | 1 | 0 | 1.20245407050858 | 1.7667 | 0.6806215376173544 | 0.6818032949350843 | 0.02998522173845591 | 0.286296 | 0.152928 | 5.456058264543547 |
| 20081214 | 1 | 0 | 1.29732317304577 | 1.7667 | 0.7343200164406917 | 0.286296 | 0.152928 | 5.88652073595332 | ||
| 20081214 | 1 | 0 | 1.11384839993109 | 1.7667 | 0.6304683307472067 | 0.286296 | 0.152928 | 5.054015714148864 | ||
| 20081214 | 0.5 | 0 | 1.073692570923 | 1.7667 | 0.6077390450687723 | 0.4567938448946341 | 0.07722848019460915 | 0.286296 | 0.152928 | 4.871811214116258 |
| 20081214 | 0.5 | 0 | 0.723786495457619 | 1.7667 | 0.40968273926394916 | 0.286296 | 0.152928 | 3.2841348265687245 | ||
| 20081214 | 0.5 | 0 | 0.623573990945431 | 1.7667 | 0.3529597503511807 | 0.286296 | 0.152928 | 2.829427011223166 | ||
| 20081214 | 0.25 | 0 | 0.233801289602549 | 1.7667 | 0.1323378556645435 | 0.272689056405399 | 0.07017906412242184 | 0.286296 | 0.152928 | 1.060858364309412 |
| 20081214 | 0.25 | 0 | 0.607872575632022 | 1.7667 | 0.3440723244648339 | 0.286296 | 0.152928 | 2.758182845739554 | ||
| 20081214 | 0.25 | 0 | 0.603605402619684 | 1.7667 | 0.3416569890868195 | 0.286296 | 0.152928 | 2.7388208217328023 | ||
| 20081214 | 0.125 | 0 | 0.283928584819607 | 1.7667 | 0.16071126100617364 | 0.2445681473362597 | 0.08385688633008602 | 0.286296 | 0.152928 | 1.2883077530686577 |
| 20081214 | 0.125 | 0 | 0.580228506978333 | 1.7667 | 0.32842503366634573 | 0.286296 | 0.152928 | 2.632749656279123 | ||
| 20110920 | 5 | 0 | 1.194408468 | 1.32838 | 0.8991466809196165 | 0.9101067348531332 | 0.010576647291933146 | 0.286296 | 0.152928 | 7.20781874849575 |
| 20110920 | 5 | 0 | 1.182546899 | 1.32838 | 0.8902173316370318 | 0.286296 | 0.152928 | 7.136238512993957 | ||
| 20110920 | 5 | 0 | 1.220710209 | 1.32838 | 0.9189465431578314 | 0.286296 | 0.152928 | 7.366540146557606 | ||
| 20110920 | 2.5 | 0 | 1.15415649 | 1.32838 | 0.8688451271473525 | 0.8350778277286407 | 0.017590033782747448 | 0.286296 | 0.152928 | 6.964912766609796 |
| 20110920 | 2.5 | 0 | 1.142320707 | 1.32838 | 0.8599351894789143 | 0.286296 | 0.152928 | 6.893488140197547 | ||
| 20110920 | 2.5 | 0 | 1.179968297 | 1.32838 | 0.8882761687167829 | 0.286296 | 0.152928 | 7.120677591970322 | ||
| 20110920 | 1.25 | 0 | 0.966975768 | 1.32838 | 0.727936108643611 | 0.6878245710356952 | 0.018012632845466232 | 0.286296 | 0.152928 | 5.835345492486475 |
| 20110920 | 1.25 | 0 | 0.947378392 | 1.32838 | 0.7131832698474835 | 0.286296 | 0.152928 | 5.717082487879144 | ||
| 20110920 | 1.25 | 0 | 0.980605521 | 1.32838 | 0.7381965408994414 | 0.286296 | 0.152928 | 5.917596072453701 | ||
| 20110920 | 0.625 | 0 | 0.749595931 | 1.32838 | 0.5642932978515184 | 0.5247991402179663 | 0.018933011496434324 | 0.286296 | 0.152928 | 4.5235375920475525 |
| 20110920 | 0.625 | 0 | 0.724587175 | 1.32838 | 0.5454667903762478 | 0.286296 | 0.152928 | 4.37261888609163 | ||
| 20110920 | 0.625 | 0 | 0.777190657 | 1.32838 | 0.5850665148526778 | 0.286296 | 0.152928 | 4.690061682215343 | ||
| 20110920 | 0.3125 | 0 | 0.529689065 | 1.32838 | 0.3987481481202668 | 0.3719036118142325 | 0.01268545454075691 | 0.286296 | 0.152928 | 3.1964799947987173 |
| 20110920 | 0.3125 | 0 | 0.519477801 | 1.32838 | 0.39106114289585814 | 0.286296 | 0.152928 | 3.134858747062353 | ||
| 20110920 | 0.3125 | 0 | 0.534286344 | 1.32838 | 0.40220896430238334 | 0.286296 | 0.152928 | 3.2242228940296243 | ||
| 20110920 | 0.15625 | 0 | 0.337133799 | 1.32838 | 0.2537931909543956 | 0.24418222079819804 | 0.008905220144216136 | 0.286296 | 0.152928 | 2.03447931112943 |
| 20110920 | 0.15625 | 0 | 0.337281148 | 1.32838 | 0.2539041147864316 | 0.286296 | 0.152928 | 2.0353685085130944 | ||
| 20110920 | 0.15625 | 0 | 0.356583826 | 1.32838 | 0.2684351059184872 | 0.286296 | 0.152928 | 2.151853118353098 | ||
| 20110920 | 0.078125 | 0 | 0.216503112 | 1.32838 | 0.16298281515831314 | 0.1549575811539436 | 0.008069592612271758 | 0.286296 | 0.152928 | 1.3065171853598037 |
| 20110920 | 0.078125 | 0 | 0.23929427 | 1.32838 | 0.1801399223113868 | 0.286296 | 0.152928 | 1.4440534975457024 | ||
| 20110920 | 0.078125 | 0 | 0.223822658 | 1.32838 | 0.1684929447898945 | 0.286296 | 0.152928 | 1.3506879714038937 | ||
| 20110920 | 5 | 0 | 0.934390096 | 1.06688 | 0.875815551889622 | 0.286296 | 0.152928 | 7.020789698826154 | ||
| 20110920 | 5 | 0 | 1.00013659 | 1.06688 | 0.9374405650119977 | 0.286296 | 0.152928 | 7.5147935520188955 | ||
| 20110920 | 5 | 0 | 1.001878988 | 1.06688 | 0.9390737365026995 | 0.286296 | 0.152928 | 7.527885525041752 | ||
| 20110920 | 2.5 | 0 | 0.836057909 | 1.06688 | 0.7836475601754649 | 0.286296 | 0.152928 | 6.281944532863857 | ||
| 20110920 | 2.5 | 0 | 0.847386344 | 1.06688 | 0.7942658443311338 | 0.286296 | 0.152928 | 6.367063756721536 | ||
| 20110920 | 2.5 | 0 | 0.870037521 | 1.06688 | 0.8154970765221955 | 0.286296 | 0.152928 | 6.537259428559957 | ||
| 20110920 | 1.25 | 0 | 0.67378012 | 1.06688 | 0.6315425539892021 | 0.286296 | 0.152928 | 5.062626997032993 | ||
| 20110920 | 1.25 | 0 | 0.700760892 | 1.06688 | 0.6568319698560288 | 0.286296 | 0.152928 | 5.265354237973245 | ||
| 20110920 | 1.25 | 0 | 0.70334809 | 1.06688 | 0.6592569829784043 | 0.286296 | 0.152928 | 5.284793841565986 | ||
| 20110920 | 0.625 | 0 | 0.502655492 | 1.06688 | 0.4711452946910618 | 0.286296 | 0.152928 | 3.7768363720884226 | ||
| 20110920 | 0.625 | 0 | 0.528157867 | 1.06688 | 0.4950489905143971 | 0.286296 | 0.152928 | 3.968455282072675 | ||
| 20110920 | 0.625 | 0 | 0.520396275 | 1.06688 | 0.4877739530218956 | 0.286296 | 0.152928 | 3.9101364863219854 | ||
| 20110920 | 0.3125 | 0 | 0.34673725 | 1.06688 | 0.3250011716406718 | 0.286296 | 0.152928 | 2.6053029922090576 | ||
| 20110920 | 0.3125 | 0 | 0.39147304 | 1.06688 | 0.36693258848230353 | 0.286296 | 0.152928 | 2.941437305859628 | ||
| 20110920 | 0.3125 | 0 | 0.370708426 | 1.06688 | 0.3474696554439112 | 0.286296 | 0.152928 | 2.78541682929916 | ||
| 20110920 | 0.15625 | 0 | 0.236174162 | 1.06688 | 0.22136900307438512 | 0.286296 | 0.152928 | 1.7745576829171565 | ||
| 20110920 | 0.15625 | 0 | 0.227208731 | 1.06688 | 0.21296559219406117 | 0.286296 | 0.152928 | 1.7071935211181462 | ||
| 20110920 | 0.15625 | 0 | 0.271655726 | 1.06688 | 0.2546263178614277 | 0.286296 | 0.152928 | 2.0411578963567485 | ||
| 20110920 | 0.078125 | 0 | 0.139391859 | 1.06688 | 0.13065373706508698 | 0.286296 | 0.152928 | 1.047357984590012 | ||
| 20110920 | 0.078125 | 0 | 0.141926257 | 1.06688 | 0.1330292600854829 | 0.286296 | 0.152928 | 1.0664008612721356 | ||
| 20110920 | 0.078125 | 0 | 0.16477621 | 1.06688 | 0.1544468075134973 | 0.286296 | 0.152928 | 1.2380900897087581 | ||
| 20110920 | 0.0390625 | 0 | 0.106867092 | 1.06688 | 0.1001678651769646 | 0.1024444076809638 | 0.009469211604614256 | 0.286296 | 0.152928 | 0.8029744556037192 |
| 20110920 | 0.0390625 | 0 | 0.093139106 | 1.06688 | 0.08730045178464306 | 0.286296 | 0.152928 | 0.6998255640358128 | ||
| 20110920 | 0.0390625 | 0 | 0.127881471 | 1.06688 | 0.11986490608128374 | 0.286296 | 0.152928 | 0.9608716082405219 | ||
| 20080813 | 0 | 0 | 0 | 0.496888 | 0 | 0 | 0 | 0.286296 | 0.152928 | 0 |
| 20080813 | 0 | 12.5 | -0.511104977 | 0.496888 | -1.0286120353077555 | -1.0057198784873638 | 0.016501112990241505 | 0.286296 | 0.152928 | -4.404500277395244 |
| 20080813 | 0 | 7.5 | -0.370708104 | 0.496888 | -0.7460596834699167 | -0.8064783760898484 | 0.08706487473787689 | 0.286296 | 0.152928 | -3.194615627663248 |
| 20080813 | 0 | 7.5 | -0.529539427 | 0.496888 | -1.0657118445202944 | 0.286296 | 0.152928 | -4.563361066846388 | ||
| 20080813 | 0 | 5 | -0.353008405 | 0.496888 | -0.7104385797201784 | -0.70164628206721 | 0.06948341477754195 | 0.286296 | 0.152928 | -3.042086631344529 |
| 20080813 | 0 | 5 | -0.343190631 | 0.496888 | -0.6906800546602052 | 0.286296 | 0.152928 | -2.957480943174124 | ||
| 20080813 | 0 | 4 | -0.289145076 | 0.496888 | -0.5819119721144402 | -0.5706204377590418 | 0.021924460990612172 | 0.286296 | 0.152928 | -2.4917377540024797 |
| 20080813 | 0 | 4 | -0.253224243 | 0.496888 | -0.5096203631401846 | 0.286296 | 0.152928 | -2.18218624104046 | ||
| 20080813 | 0 | 3 | -0.322995222 | 0.496888 | -0.6500362697428798 | -0.676148742827491 | 0.08755501506244243 | 0.286296 | 0.152928 | -2.7834449064586955 |
| 20080813 | 0 | 3 | -0.309967865 | 0.496888 | -0.6238183755695449 | 0.286296 | 0.152928 | -2.6711803030947823 | ||
| 20080813 | 0 | 2 | -0.256148522 | 0.496888 | -0.5155055505466021 | -0.6161636054253024 | 0.11037270966327536 | 0.286296 | 0.152928 | -2.2073865193517417 |
| 20080813 | 0 | 2 | -0.408206238 | 0.496888 | -0.8215256516559064 | 0.286296 | 0.152928 | -3.517759695980165 | ||
| 20080813 | 0 | 1.5 | -0.479204511 | 0.496888 | -0.9644115192960989 | -0.6629587056477988 | 0.1319525676603437 | 0.286296 | 0.152928 | -4.129594695041587 |
| 20080813 | 0 | 1.5 | -0.387358093 | 0.496888 | -0.7795682185925199 | 0.286296 | 0.152928 | -3.338098638921673 | ||
| 20080813 | 0 | 1 | -0.260251519 | 0.496888 | -0.523762938529407 | -0.44416159227157553 | 0.04927841486143834 | 0.286296 | 0.152928 | -2.242744522575905 |
| 20080813 | 0 | 1 | -0.255600519 | 0.496888 | -0.5144026802820757 | 0.286296 | 0.152928 | -2.202664046524964 | ||
| 20080813 | 0 | 0.75 | -0.183258195 | 0.496888 | -0.36881187511068897 | -0.3485874422327215 | 0.10191014440158239 | 0.286296 | 0.152928 | -1.5792465482339684 |
| 20080813 | 0 | 0.75 | -0.30586191 | 0.496888 | -0.6155550345349455 | 0.286296 | 0.152928 | -2.6357968089980846 | ||
| 20080813 | 0 | 0.25 | -0.175111685 | 0.496888 | -0.3524168122393779 | -0.33967668044968036 | 0.07115601167978769 | 0.286296 | 0.152928 | -1.5090431513400202 |
| 20080813 | 0 | 0.25 | -0.216803576 | 0.496888 | -0.43632282526444593 | 0.286296 | 0.152928 | -1.8683273566171534 | ||
| 20080813 | 0 | 0.125 | -0.009251689 | 0.496888 | -0.018619264301009485 | -0.052248985906610826 | 0.03362972160560135 | 0.286296 | 0.152928 | -0.0797273918286938 |
| 20080806 | 0 | 12.5 | -1.394043157 | 1.37363 | -1.014860739063649 | 0.286296 | 0.152928 | -4.3456174468987205 | ||
| 20080806 | 0 | 12.5 | -1.337485483 | 1.37363 | -0.9736868610906868 | 0.286296 | 0.152928 | -4.169311560200544 | ||
| 20080806 | 0 | 7.5 | -0.989358809 | 1.37363 | -0.7202513114885377 | 0.286296 | 0.152928 | -3.0841045917729346 | ||
| 20080806 | 0 | 7.5 | -0.953149034 | 1.37363 | -0.6938906648806448 | 0.286296 | 0.152928 | -2.971228724768283 | ||
| 20080806 | 0 | 5 | -1.198682066 | 1.37363 | -0.8726382402830457 | 0.286296 | 0.152928 | -3.7366229826801574 | ||
| 20080806 | 0 | 5 | -0.731908874 | 1.37363 | -0.5328282536054105 | 0.286296 | 0.152928 | -2.2815620566863104 | ||
| 20080806 | 0 | 4 | -0.792555216 | 1.37363 | -0.5769786740243006 | 0.286296 | 0.152928 | -2.470613450513271 | ||
| 20080806 | 0 | 4 | -0.84336863 | 1.37363 | -0.6139707417572418 | 0.286296 | 0.152928 | -2.629012892672641 | ||
| 20080806 | 0 | 3 | -0.698651804 | 1.37363 | -0.5086171705626698 | 0.286296 | 0.152928 | -2.177890586474623 | ||
| 20080806 | 0 | 3 | -1.26665603 | 1.37363 | -0.9221231554348698 | 0.286296 | 0.152928 | -3.948516597601626 | ||
| 20080806 | 0 | 2 | -0.485681751 | 1.37363 | -0.3535753812889934 | 0.286296 | 0.152928 | -1.5140041254733692 | ||
| 20080806 | 0 | 2 | -1.063255332 | 1.37363 | -0.7740478382097071 | 0.286296 | 0.152928 | -3.3144604584485546 | ||
| 20080806 | 0 | 1.5 | -0.7504616 | 1.37363 | -0.5463346024766494 | 0.286296 | 0.152928 | -2.3393960264513733 | ||
| 20080806 | 0 | 1.5 | -0.49659538 | 1.37363 | -0.3615204822259269 | 0.286296 | 0.152928 | -1.5480249205637036 | ||
| 20080806 | 0 | 1 | -0.428029316 | 1.37363 | -0.3116045194120688 | 0.286296 | 0.152928 | -1.334285566450168 | ||
| 20080806 | 0 | 1 | -0.586369997 | 1.37363 | -0.42687623086275056 | 0.286296 | 0.152928 | -1.827877190534604 | ||
| 20080806 | 0 | 0.75 | -0.386679785 | 1.37363 | -0.2815021403143496 | 0.286296 | 0.152928 | -1.2053876607918002 | ||
| 20080806 | 0 | 0.75 | -0.17648497 | 1.37363 | -0.1284807189709019 | 0.286296 | 0.152928 | -0.5501523829418985 | ||
| 20080806 | 0 | 0.25 | -0.184794142 | 1.37363 | -0.13452978021738024 | 0.286296 | 0.152928 | -0.5760543664143387 | ||
| 20080806 | 0 | 0.25 | -0.598129744 | 1.37363 | -0.43543730407751724 | 0.286296 | 0.152928 | -1.8645355690630636 | ||
| 20080806 | 0 | 0.125 | -0.117965569 | 1.37363 | -0.08587870751221217 | 0.286296 | 0.152928 | -0.36773125150797237 | ||
