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| Metadata | Values (examples) | ||||||||
| Asset Title | PFK Kinetic Data | ||||||||
| Uploader | Dawie van Niekerk | ||||||||
| Uploader SEEK ID | |||||||||
| Project | |||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 0 | ||||||||
| Assay Title | PFK | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of PFK. Experimental data for enzyme reaction rates. | ||||||||
| Experimentalist | Gerald Penkler | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | °C | ||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | concentration | concentration | |||||
| Compound (if concentration) | DHAP | NADH | NAD | G3P | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | |||||||||
| EC Number | 2.7.1.11 | ||||||||
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| Date | DHAP | NADH | G3P | NAD | v | Vm | v/Vm | mean (v/V) | SEM (v/V) | Vf_model | Vr_model | v_unnormalised |
| yyyymmdd | (mM) | (mM) | (mM) | (mM) | (U/mg) | (U/mg) | 1 | 1 | (M/min) | (M/min) | (fmol/28fL/min) | |
| 1.0E-8 | 0.358 | 0 | 0 | 0 | 0.0406776 | 0 | 0 | 0 | 0.012028629550321199 | 0 | ||
| 0.028 | 0.358 | 0 | 0 | 0.0037 | 0.0406776 | 0.09095915196570102 | 0.038906048468332545 | 0.02803681691423587 | 0.012028629550321199 | 0.030635190409790063 | ||
| 0.056 | 0.358 | 0 | 0 | 0.0033 | 0.0406776 | 0.08112573013157118 | 0.05426893368271276 | 0.030066595879354573 | 0.012028629550321199 | 0.027323277933055997 | ||
| 0.111 | 0.358 | 0 | 0 | 0.0094 | 0.0406776 | 0.23108541310205125 | 0.1655101460837661 | 0.04983775104787492 | 0.012028629550321199 | 0.07782994320325043 | ||
| 0.333 | 0.358 | 0 | 0 | 0.021 | 0.0406776 | 0.5162546462918166 | 0.4519922844141704 | 0.02359022959764944 | 0.012028629550321199 | 0.17387540502853818 | ||
| 1.11 | 0.358 | 0 | 0 | 0.0314 | 0.0406776 | 0.7719236139791924 | 0.6035212068804522 | 0.09018765621418946 | 0.012028629550321199 | 0.25998512942362373 | ||
| 2.22 | 0.358 | 0 | 0 | 0.0328 | 0.0406776 | 0.8063405903986469 | 0.7127981298902929 | 0.033934563704773664 | 0.012028629550321199 | 0.271576823092193 | ||
| 5.55 | 0.358 | 0 | 0 | 0.0394 | 0.0406776 | 0.9685920506617892 | 0.9192179777329358 | 0.040095457053113064 | 0.012028629550321199 | 0.32622337895830494 | ||
| 1.0E-8 | 0.358 | 0 | 0 | 0 | 0.0635657 | 0 | 0.012028629550321199 | 0 | ||||
| 0.028 | 0.358 | 0 | 0 | 0.0012 | 0.0635657 | 0.01887810564502554 | 0.012028629550321199 | 0.00635817670364351 | ||||
| 0.056 | 0.358 | 0 | 0 | 0.006 | 0.0635657 | 0.0943905282251277 | 0.012028629550321199 | 0.03179088351821755 | ||||
| 0.111 | 0.358 | 0 | 0 | 0.0121 | 0.0635657 | 0.1903542319206742 | 0.012028629550321199 | 0.06411161509507206 | ||||
| 0.333 | 0.358 | 0 | 0 | 0.0257 | 0.0635657 | 0.40430609589763034 | 0.012028629550321199 | 0.1361709510696985 | ||||
| 1.11 | 0.358 | 0 | 0 | 0.0374 | 0.0635657 | 0.5883676259366294 | 0.012028629550321199 | 0.19816317393022276 | ||||
| 2.22 | 0.358 | 0 | 0 | 0.0429 | 0.0635657 | 0.674892276809663 | 0.012028629550321199 | 0.22730481715525547 | ||||
| 5.55 | 0.358 | 0 | 0 | 0.0625 | 0.0635657 | 0.9832346690117468 | 0.012028629550321199 | 0.3311550366480995 | ||||
| 1.0E-8 | 0.358 | 0 | 0 | 0 | 0.0494867 | 0 | 0.012028629550321199 | 0 | ||||
| 0.028 | 0.358 | 0 | 0 | 0.0038 | 0.0494867 | 0.07678830877791407 | 0.012028629550321199 | 0.025862427362385764 | ||||
| 0.056 | 0.358 | 0 | 0 | 0.0038 | 0.0494867 | 0.07678830877791407 | 0.012028629550321199 | 0.025862427362385764 | ||||
| 0.111 | 0.358 | 0 | 0 | 0.011 | 0.0494867 | 0.2222819464623828 | 0.012028629550321199 | 0.0748649213121693 | ||||
| 0.333 | 0.358 | 0 | 0 | 0.022367 | 0.0494867 | 0.4519800269567379 | 0.012028629550321199 | 0.15222760863539012 | ||||
| 1.11 | 0.358 | 0 | 0 | 0.0177 | 0.0494867 | 0.3576718593076524 | 0.012028629550321199 | 0.12046446429321792 | ||||
| 2.22 | 0.358 | 0 | 0 | 0.0323 | 0.0494867 | 0.6527006246122696 | 0.012028629550321199 | 0.219830632580279 | ||||
| 5.55 | 0.358 | 0 | 0 | 0.0399 | 0.0494867 | 0.8062772421680976 | 0.012028629550321199 | 0.2715554873050505 | ||||
| 1.0E-8 | 0.358 | 0 | 0 | 0 | 0.0709646 | 0 | 0.012028629550321199 | 0 | ||||
| 0.028 | 0.358 | 0 | 0 | -0.0022 | 0.0709646 | -0.03100137251531045 | 0.012028629550321199 | -0.010441312715069005 | ||||
| 0.056 | 0.358 | 0 | 0 | -0.0025 | 0.0709646 | -0.03522883240376187 | 0.012028629550321199 | -0.011865128085305685 | ||||
| 0.111 | 0.358 | 0 | 0 | 0.0013 | 0.0709646 | 0.018318992849956174 | 0.012028629550321199 | 0.0061698666043589565 | ||||
| 0.333 | 0.358 | 0 | 0 | 0.0309 | 0.0709646 | 0.43542836851049677 | 0.012028629550321199 | 0.1466529831343783 | ||||
| 1.11 | 0.358 | 0 | 0 | 0.0494 | 0.0709646 | 0.6961217282983346 | 0.012028629550321199 | 0.23445493096564035 | ||||
| 2.22 | 0.358 | 0 | 0 | 0.0509 | 0.0709646 | 0.7172590277405918 | 0.012028629550321199 | 0.24157400781682378 | ||||
| 5.55 | 0.358 | 0 | 0 | 0.0652 | 0.0709646 | 0.9187679490901096 | 0.012028629550321199 | 0.30944254046477226 | ||||
| 5.55 | 1.0E-8 | 0 | 0 | 1.0E-4 | 0.0284571 | 0.0035140615171609197 | 0.0033696368610027574 | 4.869615991000674E-4 | 0.012028629550321199 | 0.0011835416377951149 | ||
| 5.55 | 0.021 | 0 | 0 | 0.0018 | 0.0284571 | 0.06325310730889655 | 0.08502249008316748 | 0.012746166290303314 | 0.012028629550321199 | 0.021303749480312065 | ||
| 5.55 | 0.043 | 0 | 0 | 0.0044 | 0.0284571 | 0.15461870675508047 | 0.25414679006185004 | 0.08179656967459803 | 0.012028629550321199 | 0.05207583206298505 | ||
| 5.55 | 0.115 | 0 | 0 | 0.0151 | 0.0284571 | 0.5306232890912989 | 0.5168282190100197 | 0.08054664026482025 | 0.012028629550321199 | 0.1787147873070623 | ||
| 5.55 | 0.143 | 0 | 0 | 0.0159 | 0.0284571 | 0.5587357812285862 | 0.6098949513036787 | 0.04440311921632041 | 0.012028629550321199 | 0.18818312040942325 | ||
| 5.55 | 0.215 | 0 | 0 | 0.0192 | 0.0284571 | 0.6746998112948965 | 0.6270935596681443 | 0.046814921975221184 | 0.012028629550321199 | 0.22723999445666201 | ||
| 5.55 | 0.358 | 0 | 0 | 0.0186 | 0.0284571 | 0.653615442191931 | 0.7062497525454141 | 0.053535301206027654 | 0.012028629550321199 | 0.22013874462989133 | ||
| 5.55 | 1.0E-8 | 0 | 0 | 1.3E-4 | 0.0277071 | 0.004691938167473319 | 0.012028629550321199 | 0.001580252410507385 | ||||
| 5.55 | 0.021 | 0 | 0 | 0.00174 | 0.0277071 | 0.06279978778002751 | 0.012028629550321199 | 0.021151070725252692 | ||||
| 5.55 | 0.043 | 0 | 0 | 0.0042 | 0.0277071 | 0.1515856946414457 | 0.012028629550321199 | 0.051054308647161664 | ||||
| 5.55 | 0.115 | 0 | 0 | 0.01463 | 0.0277071 | 0.5280235030010358 | 0.012028629550321199 | 0.17783917512094646 | ||||
| 5.55 | 0.143 | 0 | 0 | 0.01545 | 0.0277071 | 0.5576188052881753 | 0.012028629550321199 | 0.18780692109491615 | ||||
| 5.55 | 0.215 | 0 | 0 | 0.01865 | 0.0277071 | 0.6731126678721339 | 0.012028629550321199 | 0.2267054419689441 | ||||
| 5.55 | 0.358 | 0 | 0 | 0.01806 | 0.0277071 | 0.6518184869582165 | 0.012028629550321199 | 0.2195335271827952 | ||||
| 0.22 | 0.358 | 0 | 0 | 0.0168 | 0.0587787 | 0.28581782176196474 | 0.3038245588889156 | 0.006670609879875555 | 0.012028629550321199 | 0.09626390751192339 | ||
| 0.22 | 0.358 | 0.083 | 0 | 0.0184 | 0.0587787 | 0.3130385666916757 | 0.29379096774464897 | 0.017658816189992543 | 0.012028629550321199 | 0.10543189870353514 | ||
| 0.22 | 0.358 | 0.165 | 0 | 0.0147 | 0.0587787 | 0.2500905940417192 | 0.3688029719768563 | 0.0030961165299730753 | 0.012028629550321199 | 0.08423091907293298 | ||
| 0.22 | 0.358 | 0.33 | 0 | 0.0133 | 0.0587787 | 0.2262724422282221 | 0.2636833779956599 | 0.003311679413071491 | 0.012028629550321199 | 0.07620892678027268 | ||
| 0.22 | 0.358 | 0.99 | 0 | 0.0133 | 0.0587787 | 0.2262724422282221 | 0.21544604997765088 | 0.0013627605757964706 | 0.012028629550321199 | 0.07620892678027268 | ||
| 0.22 | 0.358 | 3.33 | 0 | 0.0138 | 0.0587787 | 0.23477892501875677 | 0.20829340073437633 | 0.023354128610858595 | 0.012028629550321199 | 0.07907392402765136 | ||
| 0.22 | 0.358 | 6.66 | 0 | 0.0118 | 0.0587787 | 0.2007529938566181 | 0.1554998067551993 | 0.029728189077089497 | 0.012028629550321199 | 0.06761393503813666 | ||
| 0.22 | 0.358 | 0 | 0 | 0.0307 | 0.102621 | 0.29915904152171585 | 0.012028629550321199 | 0.10075725203862858 | ||||
| 0.22 | 0.358 | 0.083 | 0 | 0.0285 | 0.102621 | 0.2777209343116906 | 0.012028629550321199 | 0.09353686264172359 | ||||
| 0.22 | 0.358 | 0.165 | 0 | 0.0263 | 0.102621 | 0.25628282710166533 | 0.012028629550321199 | 0.08631647324481861 | ||||
| 0.22 | 0.358 | 0.33 | 0 | 0.0239 | 0.102621 | 0.23289580105436508 | 0.012028629550321199 | 0.07843968481183136 | ||||
| 0.22 | 0.358 | 0.99 | 0 | 0.0235 | 0.102621 | 0.22899796337981504 | 0.012028629550321199 | 0.07712688673966682 | ||||
| 0.22 | 0.358 | 3.33 | 0 | 0.0193 | 0.102621 | 0.1880706677970396 | 0.012028629550321199 | 0.06334250698193913 | ||||
| 0.22 | 0.358 | 6.66 | 0 | 0.0145 | 0.102621 | 0.14129661570243907 | 0.012028629550321199 | 0.047588930115964635 | ||||
| 0.22 | 0.358 | 16.7 | 0 | 0.013 | 0.102621 | 0.1266797244228764 | 0.10321487989960933 | 0.02388807807867119 | 0.012028629550321199 | 0.0426659373453476 | ||
| 5.55 | 0.22 | 0 | 0 | 0.0313 | 0.051233 | 0.6109343587141101 | 0.5803948299753133 | 0.03053952873879673 | 0.012028629550321199 | 0.20576368625498212 | ||
| 5.55 | 0.22 | 0 | 0.5 | 0.0313 | 0.051233 | 0.6109343587141101 | 0.5593477371050162 | 0.05158662160909394 | 0.012028629550321199 | 0.20576368625498212 | ||
| 5.55 | 0.22 | 0 | 1 | 0.0162 | 0.051233 | 0.3162024476411688 | 0.5145863593112447 | 0.1983839116700758 | 0.012028629550321199 | 0.10649749895625273 | ||
| 5.55 | 0.22 | 0 | 1.7 | 0.0198 | 0.051233 | 0.3864696582280952 | 0.28531586042159823 | 0.10115379780649698 | 0.012028629550321199 | 0.13016360983542 | ||
| 5.55 | 0.22 | 0 | 2.8 | 0.0133 | 0.051233 | 0.25959830577947807 | 0.14295358593367485 | 0.11664471984580319 | 0.012028629550321199 | 0.08743313185914575 | ||
| 5.55 | 0.22 | 0 | 3.5 | 0.0121 | 0.051233 | 0.23617590225050258 | 0.15360492034387796 | 0.08257098190662462 | 0.012028629550321199 | 0.07954442823275666 | ||
| 5.55 | 0.22 | 0 | 5.2 | 0.0139 | 0.051233 | 0.27130950754396577 | 0.28693073377724465 | 0.015621226233278884 | 0.012028629550321199 | 0.09137748367234029 | ||
| 5.55 | 0.22 | 0 | 8.7 | 0.0082 | 0.051233 | 0.16005309078133237 | 0.160484 | 0 | 0.012028629550321199 | 0.05390614144699213 | ||
| 5.55 | 0.22 | 0 | 0 | 0.0209 | 0.03801 | 0.5498553012365166 | 0.012028629550321199 | 0.1851921602959212 | ||||
| 5.55 | 0.22 | 0 | 0.5 | 0.0193 | 0.03801 | 0.5077611154959222 | 0.012028629550321199 | 0.17101477003403254 | ||||
| 5.55 | 0.22 | 0 | 1 | 0.0271 | 0.03801 | 0.7129702709813206 | 0.012028629550321199 | 0.24012954756073993 | ||||
| 5.55 | 0.22 | 0 | 1.7 | 0.007 | 0.03801 | 0.1841620626151013 | 0.012028629550321199 | 0.062026082395763085 | ||||
| 5.55 | 0.22 | 0 | 2.8 | 0.001 | 0.03801 | 0.026308866087871613 | 0.012028629550321199 | 0.008860868913680441 | ||||
| 5.55 | 0.22 | 0 | 3.5 | 0.0027 | 0.03801 | 0.07103393843725335 | 0.012028629550321199 | 0.02392434606693719 | ||||
| 5.55 | 0.22 | 0 | 5.2 | 0.0115 | 0.03801 | 0.30255196001052354 | 0.012028629550321199 | 0.10189999250732508 | ||||
| 5.55 | 0.22 | 0 | 8.7 | 0.0061 | 0.03801 | 0.16048408313601684 | 0.16048408313601684 | 0 | 0.012028629550321199 | 0.05405130037345069 | ||
| 5.55 | 1.0E-8 | 0 | 0 | 1.23358574194645E-4 | 0.046487999999999995 | 0.002653557352319846 | 0.012028629550321199 | 8.937224346844243E-4 | ||||
| 5.55 | 0.021 | 0 | 0 | 0.0050988210667123725 | 0.046487999999999995 | 0.1096803705625618 | 0.012028629550321199 | 0.036940527300292285 | ||||
| 5.55 | 0.043 | 0 | 0 | 0.023040640357912655 | 0.046487999999999995 | 0.49562554547222204 | 0.012028629550321199 | 0.16692749030051454 | ||||
| 5.55 | 0.072 | 0 | 0 | 0.0300994921034957 | 0.046487999999999995 | 0.6474679939660924 | 0.477448587797222 | 0.17001940616887048 | 0.012028629550321199 | 0.21806827406301638 | ||
| 5.55 | 0.115 | 0 | 0 | 0.03258037009563263 | 0.046487999999999995 | 0.7008339807183066 | ||||||
| 5.55 | 0.143 | 0 | 0 | 0.034499281249771675 | 0.046487999999999995 | 0.7421115395321735 | ||||||
| 5.55 | 0.358 | 0 | 0 | 0.03780940299066157 | 0.046487999999999995 | 0.8133153284860949 | ||||||
| 5.55 | 1.0E-8 | 0 | 0 | 2.0372654958222716E-4 | 0.0777882 | 0.002618990407056946 | ||||||
| 5.55 | 0.021 | 0 | 0 | 0.008117719437198884 | 0.0777882 | 0.10435669468118408 | ||||||
| 5.55 | 0.043 | 0 | 0 | 0.016705577065741262 | 0.0777882 | 0.21475721337865206 | ||||||
| 5.55 | 0.072 | 0 | 0 | 0.02391436266634253 | 0.0777882 | 0.3074291816283515 | ||||||
| 5.55 | 0.115 | 0 | 0 | 0.02394570521243215 | 0.0777882 | 0.3078321032294377 | ||||||
| 5.55 | 0.143 | 0 | 0 | 0.04520378709768348 | 0.0777882 | 0.5811136791657794 | ||||||
| 5.55 | 0.215 | 0 | 0 | 0.041497531022591856 | 0.0777882 | 0.5334681998374028 | ||||||
| 0.22 | 0.358 | 0 | 0 | 0.0100334861186976 | 0.0263815 | 0.3803228064627713 | ||||||
| 0.22 | 0.358 | 0.083 | 0 | 0.008796637138718028 | 0.0263815 | 0.33343961255872595 | ||||||
| 0.22 | 0.358 | 0.165 | 0 | 0.008963830242002523 | 0.0263815 | 0.3397771257131901 | ||||||
| 0.22 | 0.358 | 0.33 | 0 | 0.009151922483197576 | 0.0263815 | 0.34690682801196204 | ||||||
| 0.22 | 0.358 | 0.99 | 0 | 0.008817536276628598 | 0.0263815 | 0.33423180170303424 | ||||||
| 0.22 | 0.358 | 3.33 | 0 | 0.009013228204336579 | 0.0263815 | 0.34164957278155444 | ||||||
| 0.22 | 0.358 | 6.66 | 0 | 0.0064445341629657685 | 0.0263815 | 0.24428232522660837 | ||||||
| 0.22 | 0.358 | 16.7 | 0 | 0.003507255325717817 | 0.0263815 | 0.13294374185386792 | ||||||
| 0.22 | 0.35800000000000004 | 0 | 0 | 0.010864295266892305 | 0.0384074 | 0.2828698445323637 | ||||||
| 0.22 | 0.35800000000000004 | 0.083 | 0 | 0.009638903823998622 | 0.0384074 | 0.25096475741650365 | ||||||
| 0.22 | 0.35800000000000004 | 0.165 | 0 | 0.023560361247388823 | 0.0384074 | 0.6134328605265866 | ||||||
| 0.22 | 0.35800000000000004 | 0.33 | 0 | 0.006013261101210164 | 0.0384074 | 0.15656516976442467 | ||||||
| 0.22 | 0.35800000000000004 | 0.99 | 0 | 0.0018317707136039434 | 0.0384074 | 0.04769317146185223 | ||||||
| 0.22 | 0.35800000000000004 | 3.33 | 0 | 0.00493946447599388 | 0.0384074 | 0.12860710373505835 | ||||||
| 0.22 | 0.35800000000000004 | 6.66 | 0 | 0.003511946609530308 | 0.0384074 | 0.09143932183720606 | ||||||
| 0.22 | 0.35800000000000004 | 16.7 | 0 | 0.0012253914428936776 | 0.0384074 | 0.031905087115859904 | ||||||
| 0.22 | 0.358 | 0 | 0 | 0.06436125032618797 | 0.20249799999999998 | 0.317836474069808 | ||||||
| 0.22 | 0.358 | 0.165 | 0 | 0.07784660026857187 | 0.20249799999999998 | 0.38443145250112043 | ||||||
| 0.22 | 0.358 | 0.33 | 0 | 0.07204405985286554 | 0.20249799999999998 | 0.3557766489193253 | ||||||
| 0.22 | 0.358 | 0.99 | 0 | 0.04860658133111225 | 0.20249799999999998 | 0.24003487111533078 | ||||||
| 0.22 | 0.358 | 3.33 | 0 | 0.030042751982274518 | 0.20249799999999998 | 0.14836073433947258 | ||||||
| 0.22 | 0.358 | 6.66 | 0 | 0.02019467541795349 | 0.20249799999999998 | 0.09972777715312493 | ||||||
| 0.22 | 0.358 | 16.7 | 0 | 0.024569277994748794 | 0.20249799999999998 | 0.12133096620583313 | ||||||
