ALD Kinetic data

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Metadata Data

Metadata	Values (examples)

Asset Title	ALD Kinetic Data
Uploader	Dawie van Niekerk
Uploader SEEK ID
Project

ASSAY
Assay SEEK ID	0
Assay Title	ALD
Assay_type	enzymaticAssay
Technology_type	initial rate experiment
Description	Kinetic characterisation of ALD. Experimental data for enzyme reaction rates.
Experimentalist	Gerald Penkler
Date
SOP
Publication (optional)

Experimental_conditions
Item	temperature	pH	Buffer	Buffer	Buffer	Buffer
Compound (if concentration)			HEPES	MgCl	KCl	NaCl
Unit	°C		mM	mM	mM	mM
Start_value (optional)	37	7.17	20	20	10	20
End_value (optional)
Comments

Culture growth	Batch

FACTORS_STUDIED
Item	concentration	concentration	concentration
Compound (if concentration)	FBP	GAP	DHAP
Unit	mM	mM	mM
Start_value (optional)	0.00625	0	0
End_value (optional)	0.75	0	0
SD (optional)
Comments

ENZYME IDENTITY

Enzyme Name (IUBMB)	fructose-bisphosphate aldolase
EC Number	4.1.2.13

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Date	FBP	GAP	DHAP	v	ControlRate	v/ControlRate	mean (v/V)	SEM (v/V)	Vf_model	Vr_model	v_unnormalised
yyymmdd	(mM)	(mM)	(mM)	(U/mg)	(U/mg)	1	1		(M/min)	(M/min)	(fmol/28fL/min)
20080808	1.0E-11	0	0	0	0.933421	0	0	0	0.0569593		0
20080808	0.625	0	0	0.759670391897594	0.933421	0.813856118404872	0.8075884991691338	0.006267619235738208	0.0569593		1.2979868945416415
20080808	0.625	0	0	0.74796973706831	0.933421	0.8013208799333956			0.0569593		1.2779949390989274
20080808	0.375	0	0	0.822028681106332	0.933421	0.8806622961196845	0.9087303628729722	0.028068066753287654	0.0569593		1.4045334218543584
20080808	0.375	0	0	0.874427326980173	0.933421	0.9367984296262598			0.0569593		1.4940627181931085
20080808	0.25	0	0	0.800068072369597	0.933421	0.8571352823319778	0.8578469792688418	7.116969368640146E-4	0.0569593		1.3670111192340908
20080808	0.25	0	0	0.801396698102606	0.933421	0.8585586762057058			0.0569593		1.3692812337569025
20080808	0.2	0	0	0.796454446584033	0.933421	0.8532639040519048	0.8461508023028763	0.007113101749028516	0.0569593		1.3608368113217824
20080808	0.2	0	0	0.783175409488673	0.933421	0.8390377005538477			0.0569593		1.3381480027203898
20080808	0.15	0	0	0.732960041724814	0.933421	0.78524057389411	0.836510272166155	0.05126969827204503	0.0569593		1.2523490957769896
20080808	0.15	0	0	0.828672467786395	0.933421	0.8877799704382			0.0569593		1.4158851187650556
20080808	0.1	0	0	0.648225482272148	0.933421	0.6944620726040533	0.6817421921102171	0.012719880493836155	0.0569593		1.1075700588981294
20080808	0.1	0	0	0.624479475131274	0.933421	0.669022311616381			0.0569593		1.066997191513426
20080808	0.075	0	0	0.546519330648737	0.933421	0.5855014303821503	0.585021017333145	4.8041304900525716E-4	0.0569593		0.9337930454598484
20080808	0.075	0	0	0.545622475391506	0.933421	0.5845406042841398			0.0569593		0.9322606619648449
20080808	0.05	0	0	0.422718425787535	0.933421	0.4528700616201425	0.461409004717936	0.008538943097793489	0.0569593		0.7222645276235251
20080808	0.05	0	0	0.438659283398106	0.933421	0.46994794781572946			0.0569593		0.7495013720325734
20080808	0.0375	0	0	0.422153649798393	0.933421	0.4522650013213684	0.3836777338810017	0.06858726744036693	0.0569593		0.7212995409133981
20080808	0.0375	0	0	0.294112058275484	0.933421	0.31509046644063504			0.0569593		0.5025253073436977
20080808	0.0125	0	0	0.182676628653838	0.933421	0.19570657683278822	0.19570657683278822	0	0.0569593		0.31212466941017136
20080808	0.00625	0	0	0.0722647911944396	0.933421	0.07741929011072132	0.07741929011072132	0	0.0569593		0.12347295999370103
20081205	0.75	0	0	0.890652718411289	0.984147	0.9049996783115623	0.8208331449246312	0.04389128251271763	0.0569593		1.4433481489518494
20081205	0.75	0	0	0.745152020730246	0.984147	0.7571552021499288			0.0569593		1.2075568485629162
20081205	0.75	0	0	0.787656692092888	0.984147	0.8003445543124026			0.0569593		1.2764378360285003
20081205	0.449999999999999	0	0	0.780440681329217	0.984147	0.7930123054068314	0.8891778308976352	0.05355133000066363	0.0569593		1.2647439226060613
20081205	0.449999999999999	0	0	0.962588414884896	0.984147	0.9780941413070364			0.0569593		1.5599236134425964
20081205	0.449999999999999	0	0	0.882215988019132	0.984147	0.8964270459790378			0.0569593		1.4296759971209465
20081205	0.3	0	0	0.7534822772225	0.984147	0.7656196454620093	0.7983903995480768	0.02290967209065428	0.0569593		1.2210564540093984
20081205	0.3	0	0	0.829152452918172	0.984147	0.8425087440373968			0.0569593		1.3436838325189804
20081205	0.3	0	0	0.774565819491451	0.984147	0.7870428091448238			0.0569593		1.2552234094098373
20081205	0.24	0	0	0.720798713013492	0.984147	0.732409602440989	0.7929195057310879	0.04745453769599176	0.0569593		1.1680910715128765
20081205	0.24	0	0	0.87244312015818	0.984147	0.8864967531864447			0.0569593		1.4138385663856343
20081205	0.24	0	0	0.747806225248527	0.984147	0.7598521615658301			0.0569593		1.2118581223357445
20081205	0.18	0	0	0.685429504837235	0.984147	0.696470654116951	0.6328984149586024	0.03189045086618782	0.0569593		1.110773466013222
20081205	0.18	0	0	0.587189290492331	0.984147	0.596647950450828			0.0569593		0.9515701889151875
20081205	0.18	0	0	0.595976433829225	0.984147	0.6055766403080283			0.0569593		0.965810202792318
20081205	0.18	0	0	0.750444025446334	0.984147	0.7625324524144604	0.8579832620964978	0.0479431155071687	0.0569593		1.2161328120707071
20081205	0.18	0	0	0.899129847371033	0.984147	0.9136133599665832			0.0569593		1.4570857687216487
20081205	0.18	0	0	0.883571087510079	0.984147	0.8978039739084496			0.0569593		1.4318720049492195
20081205	0.12	0	0	0.552286042216287	0.984147	0.561182467879582	0.5515850729391839	0.027355004313905664	0.0569593		0.8950076951954173
20081205	0.12	0	0	0.511248293396581	0.984147	0.5194836679851496			0.0569593		0.8285039305162628
20081205	0.12	0	0	0.64001414295053	0.984147	0.6503237249623584			0.0569593		1.0371755561229568
20081205	0.12	0	0	0.529314837423064	0.984147	0.5378412345138114			0.0569593		0.8577816864131911
20081205	0.12	0	0	0.481340657902933	0.984147	0.4890942693550181			0.0569593		0.7800370820612519
20081205	0.09	0	0	0.497345529882467	0.984147	0.5053569536689814	0.49725968500016904	0.025403244151663954	0.0569593		0.805973793271293
20081205	0.09	0	0	0.558325354011067	0.984147	0.5673190631186875			0.0569593		0.9047947079330952
20081205	0.09	0	0	0.525440119054747	0.984147	0.5339041007641612			0.0569593		0.8515025077063704
20081205	0.09	0	0	0.432608702758309	0.984147	0.43957732204468336			0.0569593		0.7010644636671124
20081205	0.09	0	0	0.433163430362717	0.984147	0.4401409854043319			0.0569593		0.7019634280383469
20081205	0.06	0	0	0.465269890062376	0.984147	0.4727646277053896	0.4891080541606246	0.014689653137886673	0.0569593		0.7539935832480686
20081205	0.06	0	0	0.502617571799661	0.984147	0.5107139195665495			0.0569593		0.8145174060454751
20081205	0.06	0	0	0.493936880929015	0.984147	0.5018933969508773			0.0569593		0.800449903818435
20081205	0.06	0	0	0.512179000550261	0.984147	0.52042936731023			0.0569593		0.8300121889201404
20081205	0.06	0	0	0.432767777548768	0.984147	0.4397389592700765			0.0569593		0.7013222524770579
20081205	0.045	0	0	0.307792287712527	0.984147	0.31275031851189605	0.347830463020252	0.021926758884930328	0.0569593		0.4987930980820099
20081205	0.045	0	0	0.38200706434341	0.984147	0.38816057392179215			0.0569593		0.619061928189139
20081205	0.045	0	0	0.337149568014039	0.984147	0.3425804966270679			0.0569593		0.5463680678828442
20081205	0.015	0	0	0.113915849107701	0.984147	0.11575084728978598	0.10579734037895064	0.013849507312530979	0.0569593		0.18460644260892697
20081205	0.015	0	0	0.0604177854056627	0.984147	0.06139101720135579			0.0569593		0.09791010225016117
20081205	0.015	0	0	0.118205993103321	0.984147	0.1201100984947584			0.0569593		0.19155883972938978
20081205	0.015	0	0	0.088390546184268	0.984147	0.08981437344651562			0.0569593		0.14324138756065927
20081205	0.015	0	0	0.139670501908663	0.984147	0.14192036546233744			0.0569593		0.2263431708294097