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| Metadata | Values (examples) | ||||||||
| Asset Title | PGI Kinetic Data | ||||||||
| Uploader | Dawie van Niekerk | ||||||||
| Uploader SEEK ID | |||||||||
| Project | |||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 0 | ||||||||
| Assay Title | PGI | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of PGI. Experimental data for enzyme reaction rates. | ||||||||
| Experimentalist | Gerald Penkler | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | pH | Buffer | Buffer | Buffer | Buffer | |||
| Compound (if concentration) | HEPES | MgCl | KCl | NaCl | |||||
| Unit | °C | mM | mM | mM | mM | ||||
| Start_value (optional) | 37 | 7.17 | 20 | 20 | 10 | 20 | |||
| End_value (optional) | 37 | ||||||||
| Comments | |||||||||
| Culture growth | Batch | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | |||||||
| Compound (if concentration) | G6P | F6P | |||||||
| Unit | mM | mM | |||||||
| Start_value (optional) | 0 | 0 | |||||||
| End_value (optional) | 35 | 5 | |||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | glucose-6-phosphate isomerase | ||||||||
| EC Number | 5.3.1.9 | ||||||||
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| Date | G6P | F6P | v | ControlRate | v/ControlRate | mean (v/V) | SEM (v/V) | Vf_model | Vr_model | v_unnormalised |
| yyymmdd | (mM) | (mM) | (U/mg) | (U/mg) | 1 | 1 | (M/min) | (M/min) | (fmol/28fL/min) | |
| 20081204 | 0 | 0 | 0 | 9.01461 | 0 | 0 | 0 | 0.800428 | 0.23263 | 0 |
| 20081204 | 35 | 0 | 9.461357512 | 9.01461 | 1.0495581630264648 | 1.0375566131720249 | 0.022499242583307575 | 0.800428 | 0.23263 | 23.52268075681852 |
| 20081204 | 35 | 0 | 8.960500874 | 9.01461 | 0.9939976187544441 | 0.800428 | 0.23263 | 22.277458727562703 | ||
| 20081204 | 35 | 0 | 9.637646276 | 9.01461 | 1.0691140577351654 | 0.800428 | 0.23263 | 23.960967156135602 | ||
| 20081204 | 21 | 0 | 8.193371325 | 9.01461 | 0.9088991453873212 | 0.912581864070289 | 0.0033374280282913657 | 0.800428 | 0.23263 | 20.370233104034316 |
| 20081204 | 21 | 0 | 8.199707968 | 9.01461 | 0.9096020757414909 | 0.800428 | 0.23263 | 20.38598716788508 | ||
| 20081204 | 21 | 0 | 8.2866295 | 9.01461 | 0.9192443710820547 | 0.800428 | 0.23263 | 20.602090136781072 | ||
| 20081204 | 11.2 | 0 | 7.441721182 | 9.01461 | 0.8255178185190486 | 0.8793805366325703 | 0.028246044945020636 | 0.800428 | 0.23263 | 18.501492140363823 |
| 20081204 | 11.2 | 0 | 8.037064336 | 9.01461 | 0.8915598496218917 | 0.800428 | 0.23263 | 19.981625084768243 | ||
| 20081204 | 11.2 | 0 | 8.30303222 | 9.01461 | 0.9210639417567705 | 0.800428 | 0.23263 | 20.642870325629673 | ||
| 20081204 | 8.4 | 0 | 8.065247368 | 9.01461 | 0.8946862224766241 | 0.873263957028276 | 0.014393219485085137 | 0.800428 | 0.23263 | 20.05169330316654 |
| 20081204 | 8.4 | 0 | 7.925694043 | 9.01461 | 0.8792054279663791 | 0.800428 | 0.23263 | 19.704737984295644 | ||
| 20081204 | 8.4 | 0 | 7.625460588 | 9.01461 | 0.8459002206418248 | 0.800428 | 0.23263 | 18.95830221062105 | ||
| 20081204 | 4.2 | 0 | 7.630199089 | 9.01461 | 0.8464258674529459 | 0.8274870402601998 | 0.01047478995903892 | 0.800428 | 0.23263 | 18.970082998541546 |
| 20081204 | 4.2 | 0 | 7.44402647 | 9.01461 | 0.8257735464984065 | 0.800428 | 0.23263 | 18.507223511745543 | ||
| 20081204 | 4.2 | 0 | 7.304193285 | 9.01461 | 0.8102617068292473 | 0.800428 | 0.23263 | 18.159572409269778 | ||
| 20081204 | 2.8 | 0 | 7.23630413 | 9.01461 | 0.8027306927310223 | 0.7689423411181036 | 0.019891806577481793 | 0.800428 | 0.23263 | 17.990787441796588 |
| 20081204 | 2.8 | 0 | 6.615460946 | 9.01461 | 0.7338599169570287 | 0.800428 | 0.23263 | 16.447256717082258 | ||
| 20081204 | 2.8 | 0 | 6.943380877 | 9.01461 | 0.7702364136662596 | 0.800428 | 0.23263 | 17.262526179305592 | ||
| 20081204 | 2.1 | 0 | 6.740870824 | 9.01461 | 0.7477717642804292 | 0.7168995284691556 | 0.020072223682139057 | 0.800428 | 0.23263 | 16.75904881670475 |
| 20081204 | 2.1 | 0 | 6.123087488 | 9.01461 | 0.6792404206061051 | 0.800428 | 0.23263 | 15.2231254387773 | ||
| 20081204 | 2.1 | 0 | 6.523750663 | 9.01461 | 0.7236864005209322 | 0.800428 | 0.23263 | 16.219248029492725 | ||
| 20081204 | 1.05 | 0 | 5.151183028 | 9.01461 | 0.5714260548154607 | 0.555008349187227 | 0.01007385170388255 | 0.800428 | 0.23263 | 12.806791597707228 |
| 20081204 | 1.05 | 0 | 5.020357383 | 9.01461 | 0.5569134308638977 | 0.800428 | 0.23263 | 12.48153490190678 | ||
| 20081204 | 1.05 | 0 | 4.838011033 | 9.01461 | 0.5366855618823222 | 0.800428 | 0.23263 | 12.028188225937615 | ||
| 20081204 | 0.7 | 0 | 3.489408353 | 9.01461 | 0.38708367339241523 | 0.3785502953168985 | 0.005735755320295235 | 0.800428 | 0.23263 | 8.675313094732036 |
| 20081204 | 0.7 | 0 | 3.433873108 | 9.01461 | 0.3809230912929123 | 0.800428 | 0.23263 | 8.53724222728729 | ||
| 20081204 | 0.7 | 0 | 3.314168372 | 9.01461 | 0.36764412126536816 | 0.800428 | 0.23263 | 8.239634163493491 | ||
| 20081204 | 0.35 | 0 | 1.74721526 | 9.01461 | 0.1938203937829812 | 0.22063066004334445 | 0.01718034835467465 | 0.800428 | 0.23263 | 4.343899564337874 |
| 20081204 | 0.35 | 0 | 1.941962986 | 9.01461 | 0.21542396021569432 | 0.800428 | 0.23263 | 4.828078349570778 | ||
| 20081204 | 0.35 | 0 | 2.277519817 | 9.01461 | 0.2526476261313579 | 0.800428 | 0.23263 | 5.662334554493975 | ||
| 20081204 | 0.175 | 0 | 1.776819184 | 9.01461 | 0.19710438765515093 | 0.1882584481192198 | 0.03382878045855307 | 0.800428 | 0.23263 | 4.417500382457041 |
| 20081204 | 0.175 | 0 | 2.180865627 | 9.01461 | 0.2419256769843621 | 0.800428 | 0.23263 | 5.422034401762692 | ||
| 20081204 | 0.175 | 0 | 1.133544656 | 9.01461 | 0.12574527971814645 | 0.800428 | 0.23263 | 2.818201197118623 | ||
| 20081202 | 11.2 | 0 | 3.348119815 | 4.31009 | 0.7768097220707688 | 0.7768097220707688 | 0 | 0.800428 | 0.23263 | 17.409847062094517 |
| 20081202 | 8.4 | 0 | 3.200937819 | 4.31009 | 0.7426614801547068 | 0.6659090201132691 | 0.07675246004143763 | 0.800428 | 0.23263 | 16.644517210643606 |
| 20081202 | 8.4 | 0 | 2.539317798 | 4.31009 | 0.5891565600718315 | 0.800428 | 0.23263 | 13.204167397824925 | ||
| 20081202 | 5.6 | 0 | 4.094954443 | 4.31009 | 0.9500855998366623 | 0.9476619194726792 | 0.002423680363983105 | 0.800428 | 0.23263 | 21.29330326216968 |
| 20081202 | 5.6 | 0 | 4.074061882 | 4.31009 | 0.9452382391086961 | 0.800428 | 0.23263 | 21.184664291092272 | ||
| 20081202 | 4.2 | 0 | 4.824488676 | 4.31009 | 1.1193475486590767 | 1.0364680105287825 | 0.0828795381302942 | 0.800428 | 0.23263 | 25.08679935098645 |
| 20081202 | 4.2 | 0 | 4.110052139 | 4.31009 | 0.9535884723984883 | 0.800428 | 0.23263 | 21.371809585979364 | ||
| 20081202 | 2.8 | 0 | 3.14154056 | 4.31009 | 0.728880501335239 | 0.7610989662628855 | 0.03221846492764652 | 0.800428 | 0.23263 | 16.335658133837356 |
| 20081202 | 2.8 | 0 | 3.419269527 | 4.31009 | 0.793317431190532 | 0.800428 | 0.23263 | 17.779817574763303 | ||
| 20081202 | 2.1 | 0 | 3.10175593 | 4.31009 | 0.7196499214633569 | 0.6713284912844047 | 0.04832143017895218 | 0.800428 | 0.23263 | 16.128782525438012 |
| 20081202 | 2.1 | 0 | 2.685216504 | 4.31009 | 0.6230070611054526 | 0.800428 | 0.23263 | 13.962824285382425 | ||
| 20081202 | 0.7 | 0 | 1.165790662 | 4.31009 | 0.2704794243275663 | 0.31774382077868446 | 0.04726439645111805 | 0.800428 | 0.23263 | 6.0619805303586265 |
| 20081202 | 0.7 | 0 | 1.573218267 | 4.31009 | 0.36500821722980265 | 0.800428 | 0.23263 | 8.180558324422861 | ||
| 20081202 | 0.35 | 0 | 0.655224024 | 4.31009 | 0.15202096104721713 | 0.16846903916159525 | 0.01644807811437812 | 0.800428 | 0.23263 | 3.4070913466548536 |
| 20081202 | 0.35 | 0 | 0.797009418 | 4.31009 | 0.18491711727597338 | 0.800428 | 0.23263 | 4.144359473715239 | ||
| 20081205 | 0 | 2.5 | -2.965238382 | 3.21334 | -0.9227901130910516 | -0.9606261446553015 | 0.032848855269780104 | 0.800428 | 0.23263 | -6.010722592234398 |
| 20081205 | 0 | 2.5 | -3.297070818 | 3.21334 | -1.0260572544455302 | 0.800428 | 0.23263 | -6.683367574846583 | ||
| 20081205 | 0 | 2.5 | -2.998146047 | 3.21334 | -0.9330310664293228 | 0.800428 | 0.23263 | -6.077428475536694 | ||
| 20081205 | 0 | 0.4 | -2.649714169 | 3.21334 | -0.8245981343399703 | -0.8406701822195389 | 0.012637930209889637 | 0.800428 | 0.23263 | -5.371135391762204 |
| 20081205 | 0 | 0.4 | -2.781468866 | 3.21334 | -0.8656005483391113 | 0.800428 | 0.23263 | -5.638210355683569 | ||
| 20081205 | 0 | 0.4 | -2.672894335 | 3.21334 | -0.8318118639795353 | 0.800428 | 0.23263 | -5.41812302969166 | ||
| 20081205 | 0 | 1 | -2.826031635 | 3.21334 | -0.8794686012062216 | -0.9194003141072321 | 0.03201305729190487 | 0.800428 | 0.23263 | -5.7285418595608935 |
| 20081205 | 0 | 1 | -2.879227629 | 3.21334 | -0.8960233367773095 | 0.800428 | 0.23263 | -5.836373447366154 | ||
| 20081205 | 0 | 1 | -3.157778152 | 3.21334 | -0.9827090043381653 | 0.800428 | 0.23263 | -6.4010126790172475 | ||
| 20081205 | 0 | 0.6 | -2.905410554 | 3.21334 | -0.9041715330466119 | -0.8873498851662133 | 0.027194135848667676 | 0.800428 | 0.23263 | -5.889447864513733 |
| 20081205 | 0 | 0.6 | -2.680397618 | 3.21334 | -0.8341469057118139 | 0.800428 | 0.23263 | -5.4333326509207005 | ||
| 20081205 | 0 | 0.6 | -2.968262468 | 3.21334 | -0.9237312167402142 | 0.800428 | 0.23263 | -6.016852602607729 | ||
| 20081205 | 0 | 0.3 | -2.483541787 | 3.21334 | -0.7728848447409861 | -0.790781071616034 | 0.009349893044903747 | 0.800428 | 0.23263 | -5.034293640098677 |
| 20081205 | 0 | 0.3 | -2.58489261 | 3.21334 | -0.8044254918558259 | 0.800428 | 0.23263 | -5.239738060771782 | ||
| 20081205 | 0 | 0.3 | -2.554710949 | 3.21334 | -0.7950328782512899 | 0.800428 | 0.23263 | -5.178557957092732 | ||
| 20081205 | 0 | 0.15 | -2.09361388 | 3.21334 | -0.6515382374725364 | -0.6489622206177995 | 0.013101720510226294 | 0.800428 | 0.23263 | -4.243885525130612 |
| 20081205 | 0 | 0.15 | -2.153764058 | 3.21334 | -0.6702571336988927 | 0.800428 | 0.23263 | -4.365813676346456 | ||
| 20081205 | 0 | 0.15 | -2.008630848 | 3.21334 | -0.6250912906819696 | 0.800428 | 0.23263 | -4.071619634637704 | ||
| 20081205 | 0 | 0.05 | -1.202884307 | 3.21334 | -0.37434081267466246 | -0.39288962896342533 | 0.019867219386075972 | 0.800428 | 0.23263 | -2.4383212910701886 |
| 20081205 | 0 | 0.05 | -1.390076495 | 3.21334 | -0.4325955221047259 | 0.800428 | 0.23263 | -2.8177714966022265 | ||
| 20081205 | 0 | 0.05 | -1.194503079 | 3.21334 | -0.37173255211088774 | 0.800428 | 0.23263 | -2.4213320207315627 | ||
| 20081205 | 0 | 0.025 | -0.63225779 | 3.21334 | -0.19676031481262488 | -0.20823453478312284 | 0.008472372232266032 | 0.800428 | 0.23263 | -1.281625856976106 |
| 20081205 | 0 | 0.025 | -0.722261763 | 3.21334 | -0.22476979186765172 | 0.800428 | 0.23263 | -1.464069507100811 | ||
| 20081205 | 0 | 0.025 | -0.652865527 | 3.21334 | -0.20317349766909196 | 0.800428 | 0.23263 | -1.3233990213573041 | ||
| 20081205 | 0 | 0.0125 | -0.557847477 | 3.21334 | -0.1736036264447584 | -0.14635378733654078 | 0.027249839108217645 | 0.800428 | 0.23263 | -1.1307915253556362 |
| 20081205 | 0 | 0.0125 | -0.382721481 | 3.21334 | -0.11910394822832317 | 0.800428 | 0.23263 | -0.7758002413379349 | ||
| 20110324 | 0 | 5 | -1.966368838 | 1.98514 | -0.9905441621245857 | -0.9860103393211563 | 0.01843325896315851 | 0.800428 | 0.23263 | -6.452048076181186 |
| 20110324 | 0 | 5 | -1.889969292 | 1.98514 | -0.952058440210766 | 0.800428 | 0.23263 | -6.201365938494454 | ||
| 20110324 | 0 | 5 | -2.015767565 | 1.98514 | -1.015428415628117 | 0.800428 | 0.23263 | -6.614135145171929 | ||
| 20110324 | 0 | 2.5 | -1.851031731 | 1.98514 | -0.932443923854237 | -0.9657171314533652 | 0.01881124551539053 | 0.800428 | 0.23263 | -6.073604040173913 |
| 20110324 | 0 | 2.5 | -1.919921261 | 1.98514 | -0.9671465292120456 | 0.800428 | 0.23263 | -6.299644318536749 | ||
| 20110324 | 0 | 2.5 | -1.980298127 | 1.98514 | -0.997560941293813 | 0.800428 | 0.23263 | -6.497752849649032 | ||
| 20110324 | 0 | 1.25 | -1.882416186 | 1.98514 | -0.948253617377112 | -0.9306255269989355 | 0.013502645756428922 | 0.800428 | 0.23263 | -6.176582692292252 |
| 20110324 | 0 | 1.25 | -1.794753556 | 1.98514 | -0.9040941978903252 | 0.800428 | 0.23263 | -5.888944131146338 | ||
| 20110324 | 0 | 1.25 | -1.865096134 | 1.98514 | -0.9395287657293693 | 0.800428 | 0.23263 | -6.119752149605449 | ||
| 20110324 | 0 | 0.625 | -1.596570214 | 1.98514 | -0.8042607644800871 | -0.8092901274805135 | 0.008522441807501802 | 0.800428 | 0.23263 | -5.238665085948075 |
| 20110324 | 0 | 0.625 | -1.639544779 | 1.98514 | -0.8259088925718086 | 0.800428 | 0.23263 | -5.379673199011435 | ||
| 20110324 | 0 | 0.625 | -1.583547618 | 1.98514 | -0.7977007253896452 | 0.800428 | 0.23263 | -5.195935352927009 | ||
| 20110324 | 0 | 0.3125 | -1.331759161 | 1.98514 | -0.6708641007687115 | -0.6725488796759928 | 0.0030010042183119573 | 0.800428 | 0.23263 | -4.36976724133111 |
| 20110324 | 0 | 0.3125 | -1.346679628 | 1.98514 | -0.6783801787279486 | 0.800428 | 0.23263 | -4.418724267369515 | ||
| 20110324 | 0 | 0.3125 | -1.32687226 | 1.98514 | -0.6684023595313178 | 0.800428 | 0.23263 | -4.353732345137573 | ||
| 20110324 | 0 | 0.15625 | -0.971654921 | 1.98514 | -0.48946417935258973 | -0.6733289485880088 | 0.10165546041474122 | 0.800428 | 0.23263 | -3.188193457198203 |
| 20110324 | 0 | 0.15625 | -1.369977051 | 1.98514 | -0.6901160880340933 | 0.800428 | 0.23263 | -4.495167755662392 | ||
| 20110324 | 0 | 0.15625 | -1.668324715 | 1.98514 | -0.8404065783773437 | 0.800428 | 0.23263 | -5.4741059051818 | ||
| 20110324 | 0 | 0.078125 | -0.706475808 | 1.98514 | -0.35588210806290743 | -0.3920566944061041 | 0.02881229925241501 | 0.800428 | 0.23263 | -2.3180879343628766 |
| 20110324 | 0 | 0.078125 | -0.737078908 | 1.98514 | -0.3712981996232004 | 0.800428 | 0.23263 | -2.418502804993663 | ||
| 20110324 | 0 | 0.078125 | -0.891307563 | 1.98514 | -0.4489897755322043 | 0.800428 | 0.23263 | -2.9245577614975873 | ||
| 20110324 | 0 | 0.0390625 | -0.471904803 | 1.98514 | -0.23771865107750587 | -0.22667642382904984 | 0.006945250695388434 | 0.800428 | 0.23263 | -1.5484137144044854 |
| 20110324 | 0 | 0.0390625 | -0.45351189 | 1.98514 | -0.22845335341588 | 0.800428 | 0.23263 | -1.4880629009438127 | ||
| 20110324 | 0 | 0.0390625 | -0.424536615 | 1.98514 | -0.21385726699376367 | 0.800428 | 0.23263 | -1.392989248581259 | ||
| 20110324 | 0 | 0.01953125 | -0.294508823 | 1.98514 | -0.14835670179433189 | -0.14081232322825257 | 0.009686334999261132 | 0.800428 | 0.23263 | -0.9663421470756319 |
| 20110324 | 0 | 0.01953125 | -0.302719694 | 1.98514 | -0.1524928690167948 | 0.800428 | 0.23263 | -0.9932836513425554 | ||
| 20110324 | 0 | 0.01953125 | -0.241368009 | 1.98514 | -0.12158739887363108 | 0.800428 | 0.23263 | -0.7919765447992384 | ||
| 20110324 | 0 | 0 | 0 | 1.98514 | 0 | 0 | 0 | 0.800428 | 0.23263 | 0 |
