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| Metadata | Values (examples) | ||||||||
| Asset Title | PGK Kinetic Data | ||||||||
| Uploader | Dawie van Niekerk | ||||||||
| Uploader SEEK ID | |||||||||
| Project | |||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 0 | ||||||||
| Assay Title | PGK | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of PGK. Experimental data for enzyme reaction rates. | ||||||||
| Experimentalist | Gerald Penkler | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | pH | Buffer | Buffer | Buffer | Buffer | |||
| Compound (if concentration) | HEPES | MgCl | KCl | NaCl | |||||
| Unit | °C | mM | mM | mM | mM | ||||
| Start_value (optional) | 37 | 7.17 | 20 | 20 | 10 | 20 | |||
| End_value (optional) | |||||||||
| Comments | |||||||||
| Culture growth | Batch | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | concentration | concentration | |||||
| Compound (if concentration) | 3PG | ATP | ADP | BPG | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | 0 | 0.078125 | 0 | 0 | |||||
| End_value (optional) | 5 | 5 | 6 | 0 | |||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | phosphoglycerate kinase | ||||||||
| EC Number | 2.7.2.3 | ||||||||
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