Selected Cell
Cell:
Value:
Metadata
Data
A
B
C
D
E
F
G
H
I
J
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
| Metadata | Values (examples) | ||||||||
| Asset Title | HK Kinetic Data | ||||||||
| Uploader | Dawie van Niekerk | ||||||||
| Uploader SEEK ID | |||||||||
| Project | |||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 0 | ||||||||
| Assay Title | HK | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of HK. Experimental data for enzyme reaction rates. | ||||||||
| Experimentalist | Gerald Penkler | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | pH | Buffer | Buffer | Buffer | Buffer | |||
| Compound (if concentration) | HEPES | MgCl | KCl | NaCl | |||||
| Unit | °C | mM | mM | mM | mM | ||||
| Start_value (optional) | 37 | 7.17 | 20 | 20 | 10 | 20 | |||
| End_value (optional) | |||||||||
| Comments | |||||||||
| Culture growth | Batch | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | concentration | concentration | |||||
| Compound (if concentration) | GLC | ATP | ADP | G6P | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | 0.3125 | 0.025 | 0 | 0 | |||||
| End_value (optional) | 10 | 10 | 20 | 30 | |||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | hexokinase | ||||||||
| EC Number | 2.7.1.1 | ||||||||
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
201
202
203
| 20110631 | 3.125 | 0.625 | 0.3125 | 0 | 0.0688562 | 0.186928826447906 | 0.36835517190383205 | 0.083 | 0.8560574195045056 | |||||
| 20110631 | 3.125 | 0.625 | 0 | 0 | 0.0687614 | 0.186928826447906 | 0.36784802700916047 | 0.37481244242190476 | 0.006964415412744285 | 0.083 | 0.854878814769289 | 0.8710641161885067 | 0.016185301419217698 | |
| 20110631 | 3.125 | 0.625 | 0 | 0 | 0.0713651 | 0.186928826447906 | 0.38177685783464904 | 0.083 | 0.8872494176077244 |
