Selected Cell
Cell:
Value:
metadata
Organism_sample
Data_measurement (metabolite)
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| Metadata | Values | ||||||||
| Asset Title | Analysis of E. coli with linear electron transport chain | ||||||||
| Uploader | Katja Bettenbrock | ||||||||
| Uploader SEEK ID | Bettenbrock | ||||||||
| Project | SUMO2 | ||||||||
| ASSAY | https://seek.sysmo-db.org/assays | ||||||||
| Assay SEEK ID | 238 | ||||||||
| Assay Title | Determination of biomass and by-product formation of mutants with linear electron transport chain at different aerobiosis levels | ||||||||
| Assay_type | Levels of by-products and biomass of mutant strains at different aerobiosis levels | ||||||||
| Technology_type | metabolite concentration | ||||||||
| Description | Expression of glucose-transport systems in MG1655 and isogenic mutant strains in aerobic batch cultures | ||||||||
| Experimentalist | Sonja Steinsiek | ||||||||
| Date | |||||||||
| SOP | SUMO Evans medium | ||||||||
| Experimental_conditions | |||||||||
| Item | |||||||||
| Compound (if concentration) | |||||||||
| Unit | |||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| Comments | |||||||||
| Culture Growth | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | rate | rate | |||||||
| Compound (if concentration) | acetate formation rates, formate formation rates, glucose consumption rates | acetate formation rates, glucose consumption rates | |||||||
| Unit | mmol*g-1*h-1 | mmol*g-1*h-1 | |||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | |||||||||
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| Culture_start_date | ||||||||||
| Sample ID (internal) | Organism | NCBI_ID | Strain | Genotype | Phenotype | organism/cell part | aerobiosis | date | ||
| so121007_U2 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 0 | 2012-10-07T0:0:0+0000 | |||
| so121015_U2 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 0 | 2012-10-15T0:0:0+0000 | |||
| so120821_U1 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 20 | 2012-08-21T0:0:0+0000 | |||
| so120827_U2 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 20 | 2012-08-27T0:0:0+0000 | |||
| so210821_U2 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 50 | 2012-08-21T0:0:0+0000 | |||
| so211008_U5 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 50 | 2012-10-08T0:0:0+0000 | |||
| so_120821_U5 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 80 | 2012-08-21T0:0:0+0000 | |||
| so_120827_U5 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 80 | 2012-08-27T0:0:0+0000 | |||
| so_120821_U6 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 100 | 2012-08-21T0:0:0+0000 | |||
| so_120827_U6 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 100 | 2012-08-27T0:0:0+0000 | |||
| so_121008_U6 | E.coli | 511145 | TBE029 | ∆nuoB | whole cell | 150 | 2012-10-08T0:0:0+0000 | |||
| so_120423_U1 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 0 | 2012-04-23T0:0:0+0000 | |||
| so_120521_U1 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 0 | 2012-05-21T0:0:0+0000 | |||
| so_120611_U1 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 0 | 2012-06-11T0:0:0+0000 | |||
| so_120705_U6 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 0 | 2012-07-05T0:0:0+0000 | |||
| so_120423_U2 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 20 | 2012-04-23T0:0:0+0000 | |||
| so_120604_U1 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 20 | 2012-06-04T0:0:0+0000 | |||
| so_120705_U2 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 20 | 2012-07-05T0:0:0+0000 | |||
| so_120508_U2 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 50 | 2012-05-08T0:0:0+0000 | |||
| so_120604_U5 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 50 | 2012-06-04T0:0:0+0000 | |||
| so_120705_U2 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 50 | 2012-07-05T0:0:0+0000 | |||
| so_120508_U1 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 80 | 2012-05-08T0:0:0+0000 | |||
| so_120529_U1 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 80 | 2012-05-29T0:0:0+0000 | |||
| so_120521_U5 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 100 | 2012-05-21T0:0:0+0000 | |||
| so_120529_U5 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 100 | 2012-05-29T0:0:0+0000 | |||
| so_120521_U6 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 100 | 2012-05-21T0:0:0+0000 | |||
| so_120705_U5 | E.coli | 511145 | TBE031 | ∆nuoB ∆cydB ∆appB | whole cell | 150 | 2012-07-05T0:0:0+0000 | |||
| so_120521_U2 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 0 | 2012-05-21T0:0:0+0000 | |||
| so_120628_U6 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 0 | 2012-06-28T0:0:0+0000 | |||
| so_120423_U6 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 20 | 2012-04-23T0:0:0+0000 | |||
| so_120604_U2 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 20 | 2012-06-04T0:0:0+0000 | |||
| so_120625_U2 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 20 | 2012-06-25T0:0:0+0000 | |||
| so_120508_U6 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 50 | 2012-05-08T0:0:0+0000 | |||
| so_120604_U6 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 50 | 2012-06-04T0:0:0+0000 | |||
| so_120508_U5 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 80 | 2012-05-08T0:0:0+0000 | |||
| so_120529_U2 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 80 | 2012-05-29T0:0:0+0000 | |||
| so_120529_U6 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 100 | 2012-05-29T0:0:0+0000 | |||
| so-120625_U5 | E.coli | 511145 | TBE032 | ∆nuoB ∆cydB ∆cyoB | whole cell | 150 | 2012-06-25T0:0:0+0000 | |||
| so_121015_U1 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 0 | 2012-10-15T0:0:0+0000 | |||
| so_121126_U5 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 0 | 2012-11-26T0:0:0+0000 | |||
| so_121112_U2 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 20 | 2012-11-12T0:0:0+0000 | |||
| so_121112_U5 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 20 | 2012-11-12T0:0:0+0000 | |||
| so_121029_U2 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 50 | 2012-10-29T0:0:0+0000 | |||
| so_121112_U6 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 50 | 2012-11-12T0:0:0+0000 | |||
| so_121015_U6 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 80 | 2012-10-15T0:0:0+0000 | |||
| so_121029_U5 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 80 | 2012-10-29T0:0:0+0000 | |||
| so_121015_U6 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 100 | 2012-10-15T0:0:0+0000 | |||
| so_121029_U6 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 100 | 2012-10-29T0:0:0+0000 | |||
| so_121126_U6 | E.coli | 511145 | TBE042 | ∆nuoB ∆cyoB ∆appB | whole cell | 150 | 2012-11-26T0:0:0+0000 | |||
| Wuchskurve_110325-110803_Regulatoren |
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| Metabolite | Acetat | Acetat | Ethanol | Ethanol | Formiate | Formiate | Succinate | Succinate | Lactate | Lactate | Glucose | Glucose | |||||||||||||||||||||||||||||||||||
| Values | Average | StDev | Average | StDev | Average | StDev | Average | StDev | Average | StDev | Average | StDev | |||||||||||||||||||||||||||||||||||
| Unit | mmol*g-1*h-1 | relative unit | mmol*g-1*h-1 | relative unit | mmol*g-1*h-1 | relative unit | mmol*g-1*h-1 | relative unit | mmol*g-1*h-1 | relative unit | mmol*g-1*h-1 | relative unit | |||||||||||||||||||||||||||||||||||
| strain | aerobiosis | ||||||||||||||||||||||||||||||||||||||||||||||
| TBE029 | 0 | 7.58 | 0.22 | 6.62 | 0.27 | 2.78 | 0.67 | 0 | 9.76 | 0.35 | |||||||||||||||||||||||||||||||||||||
| TBE029 | 20 | 4.6 | 0.99 | 2.76 | 0.59 | 0 | 0 | 6.097 | 0.77 | ||||||||||||||||||||||||||||||||||||||
| TBE029 | 50 | 3.09 | 0.39 | 0.89 | 0.1 | 0 | 0 | 4.42 | 0.344 | ||||||||||||||||||||||||||||||||||||||
| TBE029 | 80 | 1.08 | 0.098 | 0.092 | 0.009 | 0 | 0 | 3.22 | 0.102 | ||||||||||||||||||||||||||||||||||||||
| TBE029 | 100 | 0.74 | 0.18 | 0.024 | 0.003 | 0 | 0 | 2.98 | 0,,07 | ||||||||||||||||||||||||||||||||||||||
| TBE029 | 150 | 0.67 | nd | 0.119 | 0.011 | 0 | 0 | 3.09 | n.d. | ||||||||||||||||||||||||||||||||||||||
| TBE031 | 0 | 7.66 | 0.64 | 7.05 | 0.5 | 5.36 | 2.59 | 1.31 | 0.18 | 0 | 10.61 | 0.85 | |||||||||||||||||||||||||||||||||||
| TBE031 | 20 | 4.17 | 0.68 | 3.48 | 0.19 | 2.82 | 0.71 | 0.3 | 0.04 | 0 | 5.98 | 0.39 | |||||||||||||||||||||||||||||||||||
| TBE031 | 50 | 1.89 | 0.33 | 1.39 | 0.87 | 0 | 0.04 | 0.07 | 0 | 4.04 | 0.49 | ||||||||||||||||||||||||||||||||||||
| TBE031 | 80 | 0.93 | 0.11 | 0.37 | 0.21 | 0 | 0 | 0 | 3.35 | 0.03 | |||||||||||||||||||||||||||||||||||||
| TBE031 | 100 | 0.61 | 0.18 | 0.45 | 0.08 | 0 | 0 | 0 | 3.13 | 0.05 | |||||||||||||||||||||||||||||||||||||
| TBE031 | 150 | 0.44 | 0.19 | 0 | 0 | 0 | 3.11 | ||||||||||||||||||||||||||||||||||||||||
| TBE032 | 0 | 6.9 | 0.24 | 5.99 | 4.85 | 2.8 | 1.02 | 0.02 | 0 | 9.84 | 0.44 | ||||||||||||||||||||||||||||||||||||
| TBE032 | 20 | 5.84 | 0.16 | 2.86 | 0.08 | 4.12 | 0.53 | 0.21 | 0.04 | 0 | 6.07 | 0.25 | |||||||||||||||||||||||||||||||||||
| TBE032 | 50 | 4.21 | 0.54 | 0.1 | 0.07 | 0.04 | 0.06 | 0 | 0 | 4.3 | 0.11 | ||||||||||||||||||||||||||||||||||||
| TBE032 | 80 | 3.69 | 0.2 | 0.102 | 0 | 0 | 0 | 4.09 | 0.31 | ||||||||||||||||||||||||||||||||||||||
| TBE032 | 100 | 3.27 | 0.07 | 0.01 | 0 | 0 | 4.1 | ||||||||||||||||||||||||||||||||||||||||
| TBE032 | 150 | 3.46 | 0 | 0 | 0 | 4.4 | |||||||||||||||||||||||||||||||||||||||||
| TBE042 | 0 | 7.74 | 1.581 | 6.82 | 0.25 | 4.66 | 2.06 | 1.88 | 0.66 | 0 | 9.67 | 0.7 | |||||||||||||||||||||||||||||||||||
| TBE042 | 20 | 4.99 | 0.21 | 3.69 | 0.32 | 0.14 | 0.52 | 0.06 | 0.07 | 0 | 6.39 | 0.03 | |||||||||||||||||||||||||||||||||||
| TBE042 | 50 | 3.06 | 0.17 | 0.96 | 0.89 | 0 | 0.02 | 0.02 | 0 | 4.26 | 0.18 | ||||||||||||||||||||||||||||||||||||
| TBE042 | 80 | 2.52 | 0.27 | 0.12 | 0.01 | 0 | 0.02 | 0.005 | 0 | 4.12 | 0.17 | ||||||||||||||||||||||||||||||||||||
| TBE042 | 100 | 2.32 | 0.39 | 0.06 | 0.002 | 0 | 0.02 | 0.002 | 0 | 4.19 | 0.17 | ||||||||||||||||||||||||||||||||||||
| TBE042 | 150 | 1.86 | 0.07 | 0 | 0.02 | 0.002 | 0 | 3.94 | 0.008 | ||||||||||||||||||||||||||||||||||||||
