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| Metadata | Values (examples) | ||||||||
| Asset Title | TPI Kinetic Data | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 228 | ||||||||
| Assay Title | TPI | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of TPI. Experimental data for enzyme reaction rates with increasing concentrations of DHAP and GAP, and inhibition with 3PG and PEP. | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | °C | ||||||||
| Start_value (optional) | 70 | ||||||||
| End_value (optional) | 70 | ||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | concentration | concentration | |||||
| Compound (if concentration) | GAP | DHAP | 3PG | PEP | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | 0.1 | 0 | 0 | 0 | |||||
| End_value (optional) | 10 | 2 | 2 | 10 | |||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | triose-phosphate isomerase | ||||||||
| EC Number | 5.3.1.1 | ||||||||
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| Concentration | |||||||||
| DHAP | GAP | 3PG | PEP | TPI act. | SD | ||||
| (mM) | (mM) | (mM) | (mM) | (U/mg) | (U/mg) | ||||
| 0.1 | 0 | 0 | 0 | -15.31 | 1.59 | ||||
| 0.25 | 0 | 0 | 0 | -44.35 | 2.18 | ||||
| 0.5 | 0 | 0 | 0 | -89.49 | 1.55 | ||||
| 1 | 0 | 0 | 0 | -156.42 | 7.8 | ||||
| 2 | 0 | 0 | 0 | -196.57 | 7.89 | ||||
| 3 | 0 | 0 | 0 | -191.87 | 8 | ||||
| 4 | 0 | 0 | 0 | -183.84 | 8.8 | ||||
| 7 | 0 | 0 | 0 | -192.2 | 13.68 | ||||
| 10 | 0 | 0 | 0 | -200.42 | 20.98 | ||||
| 0 | 2 | 0 | 0 | 222.79 | 17.5 | ||||
| 0 | 3 | 0 | 0 | 220.05 | 10.72 | ||||
| 0 | 4 | 0 | 0 | 213.44 | 2.83 | ||||
| 0 | 5 | 0 | 0 | 219.37 | 8.83 | ||||
| 0 | 1 | 0 | 0 | 214.35 | 27.32 | ||||
| 0 | 0.5 | 0 | 0 | 168.29 | 3.06 | ||||
| 0 | 0.25 | 0 | 0 | 111.97 | 5.5 | ||||
| 0 | 0.125 | 0 | 0 | 69.78 | 2.18 | ||||
| 0 | 0.05 | 0 | 0 | 41.05 | 7.68 | ||||
| 0 | 0 | 0 | 0 | 0 | |||||
| 1 | 0 | 0 | 0 | -111.4 | |||||
| 1 | 0 | 0 | 1 | -94.3 | |||||
| 1 | 0 | 0 | 5 | -48.2 | |||||
| 1 | 0 | 0 | 10 | -21.8 | |||||
| 10 | 0 | 0 | 0 | -223.125 | |||||
| 10 | 0 | 0 | 1 | -216.339285714286 | |||||
| 10 | 0 | 0 | 5 | -157.589285714286 | |||||
| 10 | 0 | 0 | 10 | -67.5 | |||||
| 1 | 0 | 1 | 0 | -50.04 | 4.9 | ||||
| 1 | 0 | 2 | 0 | -21.5 | 7.3 | ||||
| 10 | 0 | 1 | 0 | -230.7 | 22.1 | ||||
| 10 | 0 | 2 | 0 | -198.5 | 18.46 | ||||
| 1 | 0 | 1 | 1 | -30.8 | 6.4 | ||||
| 10 | 0 | 1 | 0 | -205 | |||||
| 10 | 0 | 2 | 0 | -177.2 |
