GAPDH Kinetic Data

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Metadata Data

Metadata	Values (examples)

Asset Title	GAPDH Kinetic Data
Uploader	Jacky Snoep
Uploader SEEK ID	49
Project	SulfoSys

ASSAY
Assay SEEK ID	224
Assay Title	GAPDH
Assay_type	enzymaticAssay
Technology_type	initial rate experiment
Description	Kinetic characterisation of GAPDH. Expermental data for enzyme reaction rates with increasing concentrations of BPG, NADPH, NADP, GAP and Pi.
Experimentalist	Theresa Kouril
Date
SOP
Publication (optional)

Experimental_conditions
Item	temperature
Compound (if concentration)
Unit	°C
Start_value (optional)	70
End_value (optional)	70
Comments

Culture growth	CultureGrowth

FACTORS_STUDIED
Item	concentration	concentration	concentration	concentration	concentration
Compound (if concentration)	GAP	NADP	NADPH	BPG	Pi
Unit	mM	mM	mM	mM	mM
Start_value (optional)	0.05	0.01	1.0E-9	1.0E-9	10
End_value (optional)	7.5	7	0.15	0.015	650
SD (optional)
Comments

ENZYME IDENTITY

Enzyme Name (IUBMB)	glyceraldehyde 3-phosphate dehydrogenase
EC Number	1.2.1.12

Concentration
GAP	NADP	As	Pi	NADPH	BPG	GAPDH act.	SD
(mM)	(mM)	(mM)	(mM)	(mM)	(mM)	(U/mg)	(U/mg)
0.05	5	300	0	0	0	-0.59743	0.89954
0.075	5	300	0	0	0	-0.95233	0.4333
0.25	5	300	0	0	0	-2.042	0.63319
0.5	5	300	0	0	0	-5.84467	0.68295
1	5	300	0	0	0	-10.11667	0.43409
1.5	5	300	0	0	0	-13.30333	1.91422
2.5	5	300	0	0	0	-16.55	1.01119
5	5	300	0	0	0	-19.24333	0.61517
7.5	5	300	0	0	0	-18.56667	1.20567
10	0.01	300	0	0	0	-1.21963	0.11256
10	0.05	300	0	0	0	-4.21233	0.15532
10	0.1	300	0	0	0	-6.27533	0.14525
10	0.25	300	0	0	0	-8.52227	0.34046
10	0.5	300	0	0	0	-11.00667	0.54418
10	1	300	0	0	0	-12.76333	0.30072
10	2	300	0	0	0	-14.96423	0.32652
10	5	300	0	0	0	-17.14	0.56241
10	7	300	0	0	0	-17.76333	0.46929
10	5	0	1.0E-9	0	0	0	0
10	5	0	10	0	0	-0.20522	0.05104
10	5	0	30	0	0	-0.57719	0.154
10	5	0	50	0	0	-1.13169	0.15074
10	5	0	75	0	0	-1.60825	0.15871
10	5	0	100	0	0	-2.14038	0.13638
10	5	0	125	0	0	-2.933	0.19526
10	5	0	150	0	0	-3.80158	0.25833
10	5	0	200	0	0	-4.94183	0.40499
10	5	0	250	0	0	-6.3534	0.51329
10	5	0	350	0	0	-8.68572	0.48576
10	5	0	400	0	0	-9.74358	0.1176
10	5	0	450	0	0	-10.80684	0.4507
10	5	0	500	0	0	-11.56327	0.27187
10	5	0	550	0	0	-12.14622	0.34916
10	5	0	600	0	0	-12.26051	0.15464
10	5	0	650	0	0	-12.36246	0.18417
0	0	0	0	1.0E-9	6	0
0	0	0	0	0.0075	6	1.62288	0.42866
0	0	0	0	0.01	6	3.52598	0.14156
0	0	0	0	0.02	6	4.68574	0.31875
0	0	0	0	0.025	6	6.95625	0.25737
0	0	0	0	0.035	6	7.64351	0.78333
0	0	0	0	0.05	6	8.50749	0.48214
0	0	0	0	0.075	6	9.12629	0.1862
0	0	0	0	0.1	6	10.22183	0.51186
0	0	0	0	0.15	6	10.1838411	0.44994
0	0	0	0	0.2	6	11.954273	0.67539
0	0	0	0	0.2	1.0E-9	0	0.12855
0	0	0	0	0.2	1.0E-4	1.0053041249999997	0.1686
0	0	0	0	0.2	2.0E-4	4.432709499999999	0.69035
0	0	0	0	0.2	4.0E-4	6.524260499999999	0.46404
0	0	0	0	0.2	6.0E-4	10.302654500000001	0.09705
0	0	0	0	0.2	8.0E-4	11.0704405	0.21596
0	0	0	0	0.2	0.001	11.581029	0.22086
0	0	0	0	0.2	0.002	13.869253	0.17769
0	0	0	0	0.2	0.003	15.6014865	0.52118
0	0	0	0	0.2	0.004	17.101121	0.33373
0	0	0	0	0.2	0.005	15.909732499999999	0.85568
0	0	0	0	0.2	0.01	16.7039915	0.66095
0	0	0	0	0.2	0.015	16.879861	0.56873