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| Metadata | Values (examples) | ||||||||
| Asset Title | FBPAase Kinetic Data | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 230 | ||||||||
| Assay Title | FBPAase | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of FBPAase. Expermental data for enzyme reaction rates with increasing concentrations of DHAP and GAP. | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | °C | ||||||||
| Start_value (optional) | 70 | ||||||||
| End_value (optional) | 70 | ||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | |||||||
| Compound (if concentration) | GAP | DHAP | |||||||
| Unit | mM | mM | |||||||
| Start_value (optional) | 0 | 0 | |||||||
| End_value (optional) | 2 | 2 | |||||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | |||||||||
| EC Number | 4.1.2.13 | ||||||||
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| Concentration | |||||||||
| mM GAP | DHAP | AV | SD | ||||||
| (mM) | (mM) | (U/mg) | (U/mg) | ||||||
| 0 | 2 | 0.24418076692747814 | 0.0016329478554035516 | ||||||
| 0.025 | 2 | 0.7136866431046368 | 0.0509364574112543 | ||||||
| 0.05 | 2 | 1.3198960374458275 | 0.09313530654973766 | ||||||
| 0.1 | 2 | 1.541450015159949 | 0.06669147763427642 | ||||||
| 0.2 | 2 | 1.8563394983791677 | 0.046845969824932256 | ||||||
| 0.5 | 2 | 2.3211314601368773 | 0.04667489450065924 | ||||||
| 1 | 2 | 2.3117036312979784 | 0.09021205611070901 | ||||||
| 2 | 2 | 2.2796490132457223 | 0.05740920754813998 | ||||||
| 4 | 0 | 0.24418076692747814 | 0.0016329478554035516 | ||||||
| 4 | 0.05 | 0.5600130330305869 | 0.034755330100656665 | ||||||
| 4 | 0.1 | 1.0304616920916356 | 0.13694387903925997 | ||||||
| 4 | 0.2 | 1.3604357014530926 | 0.0787378941421227 | ||||||
| 4 | 0.3 | 1.60933038280002 | 0.129364103798464 | ||||||
| 4 | 0.4 | 1.8799090704764148 | 0.12681393563191437 | ||||||
| 4 | 0.8 | 2.124089837403893 | 0.06406069213598094 | ||||||
| 4 | 1 | 2.2758778817101635 | 0.15300120147842958 | ||||||
| 4 | 2 | 2.4154097485258643 | 0.08182681988108158 | ||||||
