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| Metadata | Values (examples) | ||||||||
| Asset Title | PGK Kinetic Data | ||||||||
| Uploader | Jacky Snoep | ||||||||
| Uploader SEEK ID | 49 | ||||||||
| Project | SulfoSys | ||||||||
| ASSAY | |||||||||
| Assay SEEK ID | 222 | ||||||||
| Assay Title | PGK | ||||||||
| Assay_type | enzymaticAssay | ||||||||
| Technology_type | initial rate experiment | ||||||||
| Description | Kinetic characterisation of Phosphoglycerate kinase. Measures enzyme reaction rate with increasing concentration of ATP, ADP, BPG and 3PG | ||||||||
| Experimentalist | Theresa Kouril | ||||||||
| Date | |||||||||
| SOP | |||||||||
| Publication (optional) | |||||||||
| Experimental_conditions | |||||||||
| Item | temperature | ||||||||
| Compound (if concentration) | |||||||||
| Unit | degrees celcius | ||||||||
| Start_value (optional) | 70 | ||||||||
| End_value (optional) | 70 | ||||||||
| Comments | |||||||||
| Culture growth | CultureGrowth | ||||||||
| FACTORS_STUDIED | |||||||||
| Item | concentration | concentration | concentration | concentration | |||||
| Compound (if concentration) | 3PG | ATP | BPG | ADP | |||||
| Unit | mM | mM | mM | mM | |||||
| Start_value (optional) | 0 | 0 | 0 | 0 | |||||
| End_value (optional) | 15 | 40 | 6 | 5 | |||||
| SD (optional) | |||||||||
| Comments | |||||||||
| ENZYME IDENTITY | |||||||||
| Enzyme Name (IUBMB) | Phosphoglycerate Kinase | ||||||||
| EC Number | 2.7.2.3 | ||||||||
| Sequence Accession Number (uniprot) | P50317 | ||||||||
| Reaction | |||||||||
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| Concentration | |||||||||
| 3PG | ATP | BPG | ADP | PGK Act | SD | ||||
| (mM) | (mM) | (mM) | (mM) | (U/mg) | (U/mg) | ||||
| 0 | 10 | 0 | 0 | 0 | 0.1374434865190047 | ||||
| 0.06 | 10 | 0 | 0 | 1.366379994937048 | 0.04471986492631012 | ||||
| 0.1 | 10 | 0 | 0 | 2.5095360564839155 | 0.2449551492414605 | ||||
| 0.2 | 10 | 0 | 0 | 3.936516686989051 | 0.20284888857605468 | ||||
| 0.4 | 10 | 0 | 0 | 5.4536763689906715 | 0.25571313236354 | ||||
| 1 | 10 | 0 | 0 | 6.705425005638017 | 0.3017459955371954 | ||||
| 2 | 10 | 0 | 0 | 7.722384354133335 | 0.26831066546844856 | ||||
| 5 | 10 | 0 | 0 | 8.627277514101044 | 0.0986035946098972 | ||||
| 10 | 10 | 0 | 0 | 9.066402955213881 | 0.45947336675721195 | ||||
| 15 | 10 | 0 | 0 | 9.002360315426365 | 0.2535200095729727 | ||||
| 5 | 0 | 0 | 0 | 0 | 0.6194429062555671 | ||||
| 5 | 2 | 0 | 0 | 3.40761318115023 | 0.36281011795095813 | ||||
| 5 | 5 | 0 | 0 | 6.180928800048312 | 0.3123985061024945 | ||||
| 5 | 8 | 0 | 0 | 7.78750729713952 | 0.2899564398821185 | ||||
| 5 | 10 | 0 | 0 | 8.54681240815669 | 0.17599688382461603 | ||||
| 5 | 15 | 0 | 0 | 10.125611449966787 | 0.2840262338619678 | ||||
| 5 | 20 | 0 | 0 | 12.584093242345553 | 0.3137962692617763 | ||||
| 5 | 30 | 0 | 0 | 12.537794150210358 | 0.8973844520573516 | ||||
| 5 | 40 | 0 | 0 | 13.08412343740564 | 0.578359557645417 | ||||
| 0 | 0 | 0 | 0.2 | 0 | 0 | ||||
| 0 | 0 | 0.4 | 0.2 | -2.78773533274956 | 1.14127494395132 | ||||
| 0 | 0 | 0.6 | 0.2 | -2.87894957676591 | 1.44892362361246 | ||||
| 0 | 0 | 1 | 0.2 | -3.72268133391711 | 0.259562728898318 | ||||
| 0 | 0 | 2 | 0.2 | -6.2756676882662 | 0.789506409231592 | ||||
| 0 | 0 | 2.5 | 0.2 | -8.60834427904261 | 1.63885474267007 | ||||
| 0 | 0 | 3 | 0.2 | -9.58889740221833 | 0.346443433583266 | ||||
| 0 | 0 | 4 | 0.2 | -12.9638244308231 | 0.753100333033602 | ||||
| 0 | 0 | 5 | 0.2 | -13.5453152364273 | 0.298195253909112 | ||||
| 0 | 0 | 6 | 0.2 | -13.471203663164 | 0.90864068765937 | ||||
| 0 | 0 | 5 | 0 | 0 | 0 | ||||
| 0 | 0 | 5 | 0.005 | -4.7583430628527 | 0.9076657572957979 | ||||
| 0 | 0 | 5 | 0.01 | -6.06954782058767 | 0.34963394605305737 | ||||
| 0 | 0 | 5 | 0.02 | -7.85202617240708 | 0.13312931662392874 | ||||
| 0 | 0 | 5 | 0.05 | -11.7096285755984 | 1.3596513692352699 | ||||
| 0 | 0 | 5 | 0.1 | -13.0474374878381 | 0.18976344699871273 | ||||
| 0 | 0 | 5 | 0.2 | -14.6436867581241 | 1.219137746108125 | ||||
| 0 | 0 | 5 | 0.5 | -14.2028179120451 | 0.8539418546800315 | ||||
| 0 | 0 | 5 | 1 | -12.9448214633197 | 0.2982679047210616 | ||||
| 0 | 0 | 5 | 2 | -10.54664696439 | 0.9347605879775849 | ||||
| 0 | 0 | 5 | 3 | -9.18223389764545 | 0.6670772437021008 | ||||
| 0 | 0 | 5 | 5 | -9.11002262113252 | 1.6781288093496816 | ||||
| 5 | 10 | 0 | 0 | 8.722748958 | 0.187241838 | ||||
| 5 | 10 | 0 | 0.2 | 6.523542082 | 0.398574793 | ||||
| 5 | 10 | 0 | 0.4 | 5.917023975 | 0.150046461 | ||||
| 5 | 10 | 0 | 0.6 | 4.935483222 | 0.116058017 | ||||
| 5 | 10 | 0 | 1 | 4.259516476 | 0.084546168 | ||||
| 5 | 10 | 0 | 2 | 3.18074763 | 0.17516605 | ||||
| 5 | 1 | 0 | 0 | 1.312 | |||||
| 5 | 1 | 0 | 0.01 | 1.312 | |||||
| 5 | 1 | 0 | 0.1 | 0.8 | |||||
| 5 | 1 | 0 | 1 | 0.236 |
