Selected Cell
Cell:
Value:
metadata
Organism_sample
Data_measurement (batch)
Data_measurement (conti)
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| Metadata | Values | ||||||||
| Asset Title | Expression of glucose-transport systems in MG1655 and isogenic mutant strains in aerobic cultures | ||||||||
| Uploader | Sonja Steinsiek | ||||||||
| Uploader SEEK ID | |||||||||
| Project | SUMO2 | ||||||||
| ASSAY | https://seek.sysmo-db.org/assays | ||||||||
| Assay SEEK ID | |||||||||
| Assay Title | transcription analysis of glucose-transporters in transporter-deletion strains | ||||||||
| Assay_type | |||||||||
| Technology_type | RT-PCR | ||||||||
| Description | Expression of glucose-transport systems in MG1655 and isogenic mutant strains in aerobic cultures | ||||||||
| Experimentalist | Sonja Steinsiek | ||||||||
| Date | 02.08.12 - 14.11.12 | ||||||||
| SOP | SUMO Evans medium | SUMO chemostat conditions | SUMO mRNA isolation Epicentre | ||||||
| Experimental_conditions | |||||||||
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| Compound (if concentration) | |||||||||
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| Culture Growth | |||||||||
| FACTORS_STUDIED | |||||||||
| Item | fold-change | ||||||||
| Compound (if concentration) | mRNA | ||||||||
| Unit | dimensionless | ||||||||
| Start_value (optional) | |||||||||
| End_value (optional) | |||||||||
| SD (optional) | |||||||||
| Comments | Samples were normalized to the reference genes recA, rpoD an atpA and to the expression level of the wild-type strain under the same conditions. | ||||||||
| Relative quantification and error propagation were calculated eith the software program qBasePLUS (Biogazelle). | |||||||||
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| Culture_start_date | ||||||||||
| Sample ID (internal) | Organism | NCBI_ID | Strain | Genotype | Phenotype | organism/cell part | aerobiosis | date | Chemstat ID | |
| Multi1_so110802_batch | E.coli | 511145 | MG1655 | DgalP | whole cell | aerobic | 2011-08-02T0:0:0+0000 | Multi 1 | ||
| Multi1_so110802_conti | E.coli | 511145 | MG1655 | DgalP | whole cell | aerobic | 2011-08-02T0:0:0+0000 | Multi 1 | ||
| Multi1_so110809_batch | E.coli | 511145 | MG1655 | wild-type | whole cell | aerobic | 2011-08-09T0:0:0+0000 | Multi 1 | ||
| Multi1_so110809_conti | E.coli | 511145 | MG1655 | wild-type | whole cell | aerobic | 2011-08-09T0:0:0+0000 | Multi 1 | ||
| Multi2_so110809_batch | E.coli | 511145 | MG1655 | DmglBAC | whole cell | aerobic | 2011-08-09T0:0:0+0000 | Multi 2 | ||
| Multi2_so110809_conti | E.coli | 511145 | MG1655 | DmglBAC | whole cell | aerobic | 2011-08-09T0:0:0+0000 | Multi 2 | ||
| Multi6_so110809_batch | E.coli | 511145 | MG1655 | DmglBAC-DgalP | whole cell | aerobic | 2011-08-09T0:0:0+0000 | Multi 6 | ||
| Multi6_so110809_conti | E.coli | 511145 | MG1655 | DmglBAC-DgalP | whole cell | aerobic | 2011-08-09T0:0:0+0000 | Multi 6 | ||
| Multi3_so110816_batch | E.coli | 511145 | MG1655 | DmalEFG | whole cell | aerobic | 2011-08-16T0:0:0+0000 | Multi 3 | ||
| Multi3_so110816_conti | E.coli | 511145 | MG1655 | DmalEFG | whole cell | aerobic | 2011-08-16T0:0:0+0000 | Multi 3 | ||
| Multi4_so110816_batch | E.coli | 511145 | MG1655 | DmanXYZ | whole cell | aerobic | 2011-08-16T0:0:0+0000 | Multi 4 | ||
| Multi4_so110816_conti | E.coli | 511145 | MG1655 | DmanXYZ | whole cell | aerobic | 2011-08-16T0:0:0+0000 | Multi 4 | ||
| Multi1_so111011_batch | E.coli | 511145 | MG1655 | DmalEFG | whole cell | aerobic | 2011-10-11T0:0:0+0000 | Multi 1 | ||
| Multi1_so111011_conti | E.coli | 511145 | MG1655 | DmalEFG | whole cell | aerobic | 2011-10-11T0:0:0+0000 | Multi 1 | ||
| Multi2_so111011_batch | E.coli | 511145 | MG1655 | DmanXYZ | whole cell | aerobic | 2011-10-11T0:0:0+0000 | Multi 2 | ||
| Multi2_so111011_conti | E.coli | 511145 | MG1655 | DmanXYZ | whole cell | aerobic | 2011-10-11T0:0:0+0000 | Multi 2 | ||
| Multi3_so111114_batch | E.coli | 511145 | MG1655 | DmglBAC | whole cell | aerobic | 2011-11-14T0:0:0+0000 | Multi 3 | ||
| Multi3_so111114_conti | E.coli | 511145 | MG1655 | DmglBAC | whole cell | aerobic | 2011-11-14T0:0:0+0000 | Multi 3 | ||
| Multi4_so111114_batch | E.coli | 511145 | MG1655 | DmglBAC-DgalP | whole cell | aerobic | 2011-11-14T0:0:0+0000 | Multi 4 | ||
| Multi4_so111114_conti | E.coli | 511145 | MG1655 | DmglBAC-DgalP | whole cell | aerobic | 2011-11-14T0:0:0+0000 | Multi 4 | ||
| Multi3_so111121_batch | E.coli | 511145 | MG1655 | wild-type | whole cell | aerobic | 2011-11-21T0:0:0+0000 | Multi 3 | ||
| Multi3_so111121_conti | E.coli | 511145 | MG1655 | wild-type | whole cell | aerobic | 2011-11-21T0:0:0+0000 | Multi 3 | ||
| Multi4_so111121_batch | E.coli | 511145 | MG1655 | DgalP | whole cell | aerobic | 2011-11-21T0:0:0+0000 | Multi 4 | ||
| Multi4_so111121_conti | E.coli | 511145 | MG1655 | DgalP | whole cell | aerobic | 2011-11-21T0:0:0+0000 | Multi 4 | ||
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| Gene | aceA | aceA SE(CNRQ) | ackA | ackA SE(CNRQ) | acs | acs SE(CNRQ) | atpA | atpA SE(CNRQ) | crp | crp SE(CNRQ) | cyaA | cyaA SE(CNRQ) | galP | galP SE(CNRQ) | galR | galR SE(CNRQ) | galS | galS SE(CNRQ) | glk | glk SE(CNRQ) | lamB | lamB SE(CNRQ) | malE | malE SE(CNRQ) | malK | malK SE(CNRQ) | malT | manX | manX4 SE(CNRQ) | mglB | mglB SE(CNRQ) | mlc | mlc SE(CNRQ) | ompC | ompC SE(CNRQ) | ompF | ompF SE(CNRQ) | poxB | poxB SE(CNRQ) | ptsG | ptsG SE(CNRQ) | ptsH | ptsH SE(CNRQ) | recA | recA SE(CNRQ) | rpoD2 | rpoD2 SE(CNRQ) | |||
| Values | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | ||
| Unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | ||
| MG1655 | 1 | 0.14343604767611537 | 1 | 0.09764598122042634 | 1 | 0.13582923925221865 | 1 | 0.08365869600961796 | 1 | 0.11964722043637549 | 1 | 0.12040767570140924 | 1 | 0.22471801856047124 | 1 | 0.15990129331562486 | 1 | 0.25460581370771623 | 1 | 0.1322220706946465 | 1 | 0.12647325097285528 | 1 | 0.16125097780045244 | 1 | 0.1732154266361001 | 1 | 0.11335662129347458 | 1 | 0.09502395936802462 | 1 | 0.12267526199482016 | 1 | 0.15172579707478995 | 1 | 0.12088300926932594 | 1 | 0.1223477149578965 | 1 | 0.12850899111931793 | 1 | 0.15803995943049487 | 1 | 0.1269407372233138 | 1 | 0.17947138668465118 | ||||
| DmglBAC | 0.9350962570270728 | 0.17389716270947408 | 0.5540845941810038 | 0.045744047607221894 | 1.1724985723634191 | 0.17110986706976716 | 1.0872891558212223 | 0.09352250006356894 | 0.7862060328090305 | 0.11676907014833067 | 0.9637231765445463 | 0.10841444060758933 | 1.2763547775179054 | 0.23027845054584212 | 0.9545711879999937 | 0.13030266849452354 | 0.9950105439766628 | 0.2512186166611049 | 0.8845383493561018 | 0.09269153103452034 | 0.7898029926724779 | 0.12058069899683653 | 0.9371267519618547 | 0.1259363285896784 | 1.0809238517673303 | 0.2745742417671411 | 1.1107085295531198 | 0.13051394316761458 | 0.65 | 0.195 | 0.0010875238553656702 | 1.3983101249557712E-4 | 1.5180177224813909 | 0.26729357032246526 | 0.8209930163950269 | 0.11826523997192237 | 0.8687331825559739 | 0.11696442241271052 | NaN | NaN | 1.2950227212994658 | 0.13436101474633677 | 0.9600074967203478 | 0.151164226215682 | 0.7802173438181323 | 0.09299918666759685 | 1.1787978720698817 | 0.2415832292842914 | ||
| DgalP | 1.2942750857167111 | 0.1838751667962808 | 1.0750509175756289 | 0.0894842522136469 | 1.4337439625796995 | 0.2330438316723629 | 1.070755987705565 | 0.14924256403138392 | 1.3231445062676659 | 0.13358342418981276 | 1.2119108983937685 | 0.13252863497433703 | 4.0523119462747715E-4 | 9.254472798593976E-5 | 1.1338608443674165 | 0.25572547795663386 | 1.2714045467597028 | 0.2506932340457438 | 0.9290696462002302 | 0.14815195197696313 | 0.7297923963949516 | 0.14572953719712733 | 1.0358240047348122 | 0.12963103705601842 | 0.9779732649220041 | 0.08610895711336577 | 1.0150131350589984 | 0.16121961140030341 | 1.1161323199761428 | 0.12259929197407535 | 1.2599265721388195 | 0.20389957008151824 | 1.1620899343373432 | 0.21281229245268754 | 1.2125751476478135 | 0.14916520873685912 | 1.2886044826113603 | 0.11325307775310668 | 1.2913623674260337 | 0.3048396034297198 | 1.0136175082458312 | 0.19705484372767562 | 0.8762727553643022 | 0.1419230626703686 | 1.0657864127283 | 0.19342430661404472 | ||||
| DmglBAC-DgalP | 1.7782896160061366 | 0.23345856719564909 | 1.0785441479857742 | 0.09292250553719832 | 2.697152934832438 | 0.7529586353846766 | 0.9700055445865366 | 0.10161403833503406 | 1.2929282402805247 | 0.15461807998619334 | 0.9910613018173887 | 0.1582566669391063 | 5.874070942296913E-4 | 9.952134228938173E-5 | 1.218375871633081 | 0.14029750631649898 | 1.8523304979251496 | 0.35164319061162885 | 0.8590469875081054 | 0.12424350403592323 | 0.8104292221010848 | 0.139530209195061 | 1.4791542569956226 | 0.20113911654450942 | 0.9945012948040428 | 0.19398624678300472 | 1.2739978545147181 | 0.25113632148859416 | 0.8911062406701554 | 0.11007203929508855 | 0.0010624879388239602 | 2.3085852663145E-4 | 1.5723098077709503 | 0.186005312574919 | 1.282138525474663 | 0.2003414042626321 | 1.3738305385023581 | 0.16487379157000717 | NaN | NaN | 1.2487587820080333 | 0.15357688425396515 | 1.3304257918789606 | 0.2511646094510287 | 0.8446762068121422 | 0.13611733912924143 | 1.2204936446851857 | 0.23945886973516778 | ||
| DmalEFG | 1.2471495031602573 | 0.17014829634183165 | 0.6401949949546082 | 0.05422120895443228 | 1.518754273349775 | 0.20712447609285914 | 1.0256425832625453 | 0.14481085177236847 | 0.963426254553861 | 0.1140256967050538 | 1.230738239020046 | 0.12367842913672364 | 1.0428499607634818 | 0.29417229955017876 | 1.5372274260824759 | 0.24549338355572442 | 1.2171186889430776 | 0.30246009156843895 | 1.1374394217551322 | 0.12001465759361725 | 1.4524106441775853 | 0.13411172010438877 | 0.002144239986336041 | 6.709714372825803E-4 | 2.7444885032366133 | 0.42843022157153643 | 1.1205211327139397 | 0.10165527126625384 | 0.7098005714047582 | 0.0660666979520398 | 1.1609971672581545 | 0.14123011736952812 | 1.5497479228098272 | 0.32815543654999957 | 0.9513628829674583 | 0.09801714351388588 | 0.9835455026110839 | 0.08601798602782001 | 1.2236319840458203 | 0.19595704077308262 | 1.1874435675905555 | 0.20745588120167044 | 0.8512288974291474 | 0.11161932677903381 | 1.1454011044889916 | 0.24136129987269245 | ||||
| DmanXYZ | 1.4857760842884058 | 0.21725824920653908 | 0.6615012671921612 | 0.31422450853368383 | 1.525027648938736 | 0.14355417902111162 | 1.046054399611146 | 0.13777391185742788 | 1.2019910626711452 | 0.13526947532555372 | 1.325718557058615 | 0.13794338099797768 | 1.1982037225122406 | 0.26501796124745897 | 1.4783397644741147 | 0.326526018603554 | 1.5824970010834607 | 0.2877500850831683 | 1.1731302186208712 | 0.13460020110525237 | 0.960884399116489 | 0.1418844813744858 | 1.509442457953839 | 0.16067172275795324 | 1.6388375084765339 | 0.18652259230114426 | 1.2588499240201705 | 0.21575910010755625 | 0.001340513568045738 | 2.817456068485679E-4 | 1.4173589405893305 | 0.27423188132977017 | 1.5353257022612041 | 0.2095344055360354 | 1.3491338397400041 | 0.11316310332376715 | 1.3813708824404751 | 0.1378186830547087 | 1.5153260629241947 | 0.24172718898056167 | 1.5855672294178305 | 0.3369202775653625 | 0.8247874999398128 | 0.11789680027925775 | 1.1590539709398529 | 0.22580683066520654 | ||||
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| Gene | aceA | aceA SE(CNRQ) | ackA | ackA SE(CNRQ) | acs | acs SE(CNRQ) | atpA | atpA SE(CNRQ) | crp | crp SE(CNRQ) | cyaA | cyaA SE(CNRQ) | galP | galP SE(CNRQ) | galR | galR SE(CNRQ) | galS | galS SE(CNRQ) | glk | glk SE(CNRQ) | lamB | lamB SE(CNRQ) | malE | malE SE(CNRQ) | malK | malK SE(CNRQ) | malT | manX | manX4 SE(CNRQ) | mglB | mglB SE(CNRQ) | mlc | mlc SE(CNRQ) | ompC | ompC SE(CNRQ) | ompF | ompF SE(CNRQ) | poxB | poxB SE(CNRQ) | ptsG | ptsG SE(CNRQ) | ptsH | ptsH SE(CNRQ) | recA | recA SE(CNRQ) | rpoD2 | rpoD2 SE(CNRQ) | |
| Values | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV | value | STDEV |
| Unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit | relative unit |
| MG1655 | 1 | 0.11110466269047849 | 1 | 0.11800023384580519 | 1 | 0.18903217160838032 | 1 | 0.1664453755655326 | 1 | 0.12876172209768313 | 1 | 0.23236357546226377 | 1 | 0.24376670655727448 | 1 | 0.19191520768644965 | 1 | 0.11410586970333556 | 1 | 0.18284676440943287 | 1 | 0.15823475806985948 | 1 | 0.1766218293386227 | 1 | 0.11147857024092192 | 1 | 0.11546120785309491 | 1 | 0.11505293957781706 | 1 | 0.15954385834558715 | 1 | 0.11985725525460211 | 1 | 0.11376094056443058 | 1 | 0.1159121346626773 | 1 | 0.1251065544750719 | 1 | 0.23997181018869754 | 1 | 0.11607177471870522 | 1 | 0.14681715887745905 | 1 | 0.30243055567435245 |
| DmglBAC | 0.8822654036291281 | 0.19987158079612297 | 0.7976429408839008 | 0.14909472397126575 | 0.6173599121376722 | 0.1619987597621829 | 1.0002935133497928 | 0.10990734053405388 | 0.9394733437131355 | 0.09105114719565441 | 0.9905271938129283 | 0.23064349635563383 | 1.0498454099534171 | 0.1383471154063359 | 1.1652372756335654 | 0.26531025539946795 | 0.7773483568614722 | 0.10062782353289464 | 0.6387372835685113 | 0.10430049534886185 | 0.6237390828614543 | 0.09846219275294121 | 0.9884888824556266 | 0.10277062148287001 | 0.6293947667906105 | 0.06902045813375082 | 0.9833490516647019 | 0.09366504724664951 | 0.7823974292689533 | 0.08252552347734667 | 1.0179456649065463E-4 | 1.2862575559643667E-5 | 1.2555685265046561 | 0.1213778509646357 | 0.9467189424703322 | 0.11707444986418972 | 0.9785917245613951 | 0.12256707284221964 | 0.6501422714284102 | 0.1168185504365594 | 1.0499819375036643 | 0.11307515511784998 | 0.7569624282793012 | 0.09449377843446015 | 0.8720189188429931 | 0.11468147079173227 | 1.146427618911571 | 0.2696744246575386 |
| DgalP | 1.2001909217949067 | 0.12121998430473628 | 1.0018045288375153 | 0.0936887692814837 | 1.3372596164259294 | 0.15786187204646607 | 0.9471465261234686 | 0.16405044340367567 | 0.89081006912228 | 0.15501907653293384 | 1.3625963660163103 | 0.2885778757455791 | 0.005390521738615716 | 9.598643748880881E-4 | 1.2028127836425218 | 0.16010022631407422 | 1.159252558464944 | 0.1786094086756928 | 0.881130297101857 | 0.1333192825974092 | 0.8178012294026532 | 0.08913679139835841 | 0.992522763540787 | 0.09965114348831934 | 0.9784911575642466 | 0.258252031753964 | 0.8733234284142426 | 0.11478299325052997 | 0.7788269068259185 | 0.13590283203966994 | 1.0779115282561653 | 0.08539631949434642 | 0.8463726988546364 | 0.10132259189470613 | 1.0748414878003982 | 0.10161406860567423 | 1.2988109792047635 | 0.2031099098119651 | 0.5353285328980681 | 0.051357193538852694 | 0.8628088287088326 | 0.1256717230230296 | 0.9330210988612724 | 0.14229392136909214 | 0.9950825645404292 | 0.08967990326025546 | 1.061020347338566 | 0.20045064920517022 |
| DmglBAC-DgalP | 1.6210043944149044 | 0.04391778261732567 | 1.3280707034456702 | 0.05261139805238626 | 0.9486999272694295 | 0.04447851450320409 | 1.161310685890589 | 0.03316036320691077 | 1.8444337728550344 | 0.11817575052592327 | 1.127955940973313 | 0.033336008572736296 | 0.008426613016963968 | 0.0013248349985691343 | 1.4907727343913442 | 0.041450028031441724 | 1.9506458594156486 | 0.19945383477216289 | 1.0582329929257526 | 0.031781393440738416 | 0.6869404145594985 | 0.020076506290697994 | 1.4046855706899086 | 0.03796799205961616 | 1.1253892597870017 | 0.09061126243378596 | 1.439373315535472 | 0.062059895351012515 | 1.0912848739435468 | 0.029524595852866795 | 2.5526711987693373E-4 | 1.7971384010530237E-5 | 2.3420467134792142 | 0.1387850106686973 | 1.4657832021931974 | 0.05685668796354944 | 2.2547229739411927 | 0.0933577397298206 | 0.8970997369078865 | 0.037433288943737104 | 1.1536824448950096 | 0.09097427597447966 | 1.5378753317730787 | 0.03199797265867139 | 0.7250426949709178 | 0.038948595128544275 | 1.1876486966946096 | 0.04590381129676631 |
| DmalEFG | 1.0832020200050985 | 0.08670830651141002 | 0.867292872583933 | 0.10088685693103276 | 0.9257216624313263 | 0.08759163978924792 | 0.9002970033017276 | 0.0890437629398006 | 0.9109733037296025 | 0.13728342749236047 | 1.3425675617726136 | 0.22235188460516075 | 1.1970839727745726 | 0.11114098185834101 | 1.6402552081080175 | 0.15426208708782396 | 0.8301644506747909 | 0.12023983282204961 | 0.9137290802048835 | 0.09185787286230887 | 3.6874646042942625 | 0.34967014622613607 | 9.024554440076329E-4 | 1.4801253067012453E-4 | 4.960043489651216 | 0.9349104715269909 | 0.7904270709350294 | 0.09530535839432676 | 0.9437273087856342 | 0.14507348793756755 | 1.0514886245763564 | 0.11155940450679654 | 1.2037910363328026 | 0.1790183003731605 | 0.7752685419517568 | 0.12006909695937126 | 1.0336950072433841 | 0.19470385457249395 | 0.5559947665364942 | 0.04344803671392027 | 1.2686664822299556 | 0.10476010714611507 | 1.022629162667576 | 0.2276245271505811 | 1.0045621573597516 | 0.10839253704705291 | 1.105700183102988 | 0.251785338970889 |
| DmanXYZ | 1.534052645060703 | 0.18362638213136553 | 0.8296957509578079 | 0.1690878325046013 | 0.7506838427753011 | 0.19860975475184428 | 0.9871616016291014 | 0.1807153527173117 | 0.9716183909521742 | 0.13607917059129887 | 1.0012609302947773 | 0.1621381916322109 | 1.1614955946624033 | 0.16182518375186158 | 1.3236802762979498 | 0.26728639572249396 | 1.095949780734319 | 0.13007937252936583 | 1.1856900236804795 | 0.20935739090356997 | 0.1384499794251211 | 0.14071018836993673 | 1.4807714129289895 | 0.184139789190333 | 1.4864676725457833 | 0.2590902316554914 | 1.1819032226385993 | 0.14333838577659003 | 7.670783937531392E-4 | 1.685989408807467E-4 | 0.8602878714375656 | 0.13808541483076994 | 1.19692198510502 | 0.13990317009644437 | 0.8984846962363734 | 0.1193936633653727 | 1.2186505224122508 | 0.18341215831563976 | 0.777177785201945 | 0.1159411122901587 | 1.4740345124513956 | 0.18403367946433413 | 1.0525878556819754 | 0.1272616816519632 | 0.8688761860425905 | 0.14553753345940085 | 1.1658799984611852 | 0.3678124696190267 |
