Protein report on identification of interaction partners of CitG in a SPINE experiment

Selected Cell

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Protein report on identification of interaction partners in the SPINE experiment

Peak-list generating software:
	Extract-Msn in Bioworks 3.2 (Thermo)
Search Engine: Sequest
	Version: v.27, rev. 11
	Fragment Tolerance: 1.00 Da (Monoisotopic)
	Parent Tolerance: 10 ppm (Monoisotopic)
	Fixed Modifications:
	Variable Modifications: +16 on M (Oxidation), +71 on C (Propionamide)
	Database: the SLRprot_18072003 database (extracted from SubtiList (genolist.pasteur.fr/SubtiList/), 4106 entries)
	Digestion Enzyme: Trypsin
	Max Missed Cleavages: 2
Scaffold Version: Scaffold_2_01_02
	Peptide Thresholds: 90.0% minimum
	Protein Thresholds: 99.0% minimum and 2 peptides minimum

1Bait protein
2cross linking by formaldehyde
3Proteins named multiple times (especially the bait proteins) were found in more than one gel slice.
4Protein identifier from SubtiList database and Subtwiki database
5Protein molecular weight
6Protein probality calculated by Protein Prophet
7Number of unique peptides
8 Sequence coverage

Bait1	CL2	Protein3,4	Protein name	MW5 (Da)	Protein prob.6	peptides7	SC8
CitG	( + )	Icd	isocitrate dehydrogenase	46401	1	24	0.582
CitG	( - )	CitG	fumarate hydratase	50514.7	1	23	0.418
CitG	( - )	CitG	fumarate hydratase	50514.7	1	22	0.39
CitG	( + )	CitB	aconitate hydratase	99317.3	1	22	0.306
CitG	( - )	CitG	fumarate hydratase	50514.7	1	18	0.31
CitG	( - )	DnaK	class I heat-shock protein (molecular chaperone)	65985	1	15	0.241
CitG	( + )	CitG	fumarate hydratase	50514.7	1	13	0.318
CitG	( - )	CitG	fumarate hydratase	50514.7	1	12	0.294
CitG	( + )	CitG	fumarate hydratase	50514.7	1	11	0.307
CitG	( - )	CitG	fumarate hydratase	50514.7	1	11	0.249
CitG	( - )	CitG	fumarate hydratase	50514.7	1	9	0.156
CitG	( - )	YclQ	unknown; similar to ferrichrome ABC transporter (binding protein)	34775	1	8	0.265
CitG	( - )	YqfA	resistence protein (against sublancin)	35623.6	1	8	0.302
CitG	( + )	SucC	succinyl-CoA synthetase (beta subunit)	41354.4	1	8	0.223
CitG	( + )	CitZ	citrate synthase II (major)	41712.3	1	7	0.296
CitG	( - )	YdjI	unknown	36064.1	1	7	0.238
CitG	( + )	BdhA	acetoine/ butanediol dehydrogenase	37322.7	1	7	0.249
CitG	( + )	AspB	aspartate aminotransferase	43071.1	1	7	0.267
CitG	( + )	CitG	fumarate hydratase	50514.7	1	7	0.182
CitG	( + )	PurB	adenylosuccinate lyase	49468.1	1	6	0.146
CitG	( + )	RpoA	RNA polymerase (alpha subunit)	34782.2	1	6	0.213
CitG	( + )	CitG	fumarate hydratase	50514.7	1	6	0.171
CitG	( + )	GroEL	class I heat-shock protein (chaperonin)	57406.3	1	5	0.108
CitG	( + )	YtsJ	NAD-dependent malate dehydrogenase	43649.2	1	5	0.18
CitG	( + )	TufA	elongation factor Tu	43574.9	1	4	0.149
CitG	( + )	CitG	fumarate hydratase	50514.7	1	4	0.136
CitG	( + )	PdhA	pyruvate dehydrogenase (E1 alpha subunit)	41531.4	1	4	0.124
CitG	( - )	RplA	ribosomal protein L1 (BL1)	24904.4	1	4	0.19
CitG	( + )	PheT	phenylalanyl-tRNA synthetase (beta subunit)	87928.8	1	3	0.0634
CitG	( + )	CitG	fumarate hydratase	50514.7	1	3	0.108
CitG	( + )	OxdC	oxalate decarboxylase	43548.5	1	3	0.109
CitG	( + )	GapA	glyceraldehyde-3-phosphate dehydrogenase	35814.5	1	3	0.104
CitG	( + )	YxjG	unknown	43098.5	1	3	0.0741
CitG	( + )	CitZ	citrate synthase II (major)	41712.3	1	3	0.0914
CitG	( - )	CitG	fumarate hydratase	50514.7	1	3	0.0649
CitG	( - )	CitG	fumarate hydratase	50514.7	1	3	0.0433
CitG	( + )	YoaC	unknown; similar to xylulokinase	54092.2	0.998	2	0.0637
CitG	( + )	YtkK	unknown; similar to 3-oxoacyl- acyl-carrier protein reductase	27957.2	0.998	2	0.106
CitG	( + )	SpoVK	disruption leads to the production of immature spores	36661.1	0.996	2	0.118
CitG	( + )	YueB	unknown	119968.9	0.998	2	0.0288
CitG	( + )	PncB	putative nicotinate phosphoribosyltransferase	56163.9	0.998	2	0.051
CitG	( - )	YvgJ	unknown	70731.5	0.998	2	0.0292
CitG	( - )	PdhB	pyruvate dehydrogenase (E1 beta subunit)	35456.4	0.996	2	0.0523
CitG	( - )	PksN	polyketide synthase	609580.7	1	2	0.00638
CitG	( - )	YetO	unknown; similar to cytochrome P450 / NADPH-cytochrome P450 reductase	119453.3	0.997	2	0.032
CitG	( - )	PksM	polyketide synthase	475746.3	1	2	0.00892
CitG	( - )	Smc	chromosome condensation and segregation SMC protein	135497.2	0.999	2	0.0135
CitG	( - )	SrfAA	surfactin synthetase / competence	402063.7	0.998	2	0.0137
CitG	( - )	YxjH	unknown	38281.4	0.998	2	0.0735
CitG	( - )	YoaC	unknown; similar to xylulokinase	54092.2	0.998	2	0.0637
CitG	( - )	YwqJ	unknown	66674.5	0.996	2	0.0631
CitG	( - )	GyrA	DNA gyrase (subunit A)	92084.1	0.998	2	0.0438
CitG	( - )	YwbM	elemental iron uptake system (permease)	42779.8	0.997	2	0.0468
CitG	( - )	MaeN	Na+/malate symporter	47809.2	0.997	2	0.0379
CitG	( - )	CitZ	citrate synthase II (major)	41712.3	0.997	2	0.0914
CitG	( - )	YoaC	unknown; similar to xylulokinase	54092.2	0.997	2	0.0637
CitG	( - )	TagF	CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase	88050.8	0.997	2	0.0255
CitG	( - )	Buk	probable branched-chain fatty-acid kinase (butyrate kinase)	39747.1	0.996	2	0.0551
CitG	( - )	YcgQ	unknown	33190.8	0.997	2	0.0772
CitG	( - )	YpuA	unknown	31277.2	0.997	2	0.0862
CitG	( - )	PksL	polyketide synthase	506300.2	0.999	2	0.00683
CitG	( - )	SpoIIE	serine phosphatase (sigma-F activation) / asymmetric septum formation	91954.1	0.997	2	0.029
CitG	( - )	Bpr	bacillopeptidase F	154562	0.999	2	0.0167
CitG	( - )	NadC	nicotinate-nucleotide pyrophosphorylase	31375.7	0.997	2	0.0588
CitG	( - )	PgdS	gamma-DL-glutamyl hydrolase	45230.1	0.997	2	0.092
CitG	( - )	PncB	putative nicotinate phosphoribosyltransferase	56163.9	0.995	2	0.0714
CitG	( - )	RocR	transcriptional activator of arginine utilization operons	52161.4	0.997	2	0.0716
CitG	( - )	XtmA	PBSX terminase (small subunit)	30227.3	0.997	2	0.0755
CitG	( + )	CitG	fumarate hydratase	50514.7	0.999	2	0.0563
CitG	( + )	YqbO	unknown; similar to phage-related protein	171017.3	0.999	2	0.0145
CitG	( + )	YqbO	unknown; similar to phage-related protein	171017.3	0.999	2	0.0196
CitG	( + )	YkgB	unknown	38392.1	0.998	2	0.0602
CitG	( + )	YceH	unknown; similar to toxic anion resistance protein	41656.8	0.998	2	0.0551
CitG	( + )	AckA	acetate kinase	43120.6	0.998	2	0.0734
CitG	( - )	YjbV	4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase	29106.3	0.998	2	0.0959
CitG	( - )	CitG	fumarate hydratase	50514.7	0.998	2	0.0844
CitG	( - )	DegU	two-component response regulator involved in degradative enzyme and competence regulation	25848.1	0.998	2	0.0917
CitG	( - )	YflB	unknown	10007.5	0.998	2	0.326
CitG	( - )	PksM	polyketide synthase	475746.3	1	2	0.00751




Bait1	CL2	Protein3,4	Protein name	MW5 (Da)	Protein prob.6	peptides7	SC8
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	22	0.615
Mdh	( + )	GuaB	inosine-monophosphate dehydrogenase	52973.3	1	21	0.553
Mdh	( + )	PurH	phosphoribosylaminoimidazole carboxy formyl formyltransferase / inosine-monophosphate cyclohydrolase	55721.9	1	17	0.377
Mdh	( + )	FusA	elongation factor G	76527.2	1	16	0.251
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	15	0.506
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	15	0.51
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	15	0.519
Mdh	( + )	Icd	isocitrate dehydrogenase	46401	1	15	0.395
Mdh	( + )	Hom	homoserine dehydrogenase	47476.4	1	15	0.351
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	14	0.538
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	14	0.522
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	14	0.548
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	14	0.548
Mdh	( + )	ThiC	biosynthesis of the pyrimidine moiety of thiamin	65899.9	1	13	0.281
Mdh	( + )	TufA	elongation factor Tu	43574.9	1	13	0.371
Mdh	( + )	AtpD	ATP synthase (subunit beta)	51402.1	1	13	0.406
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	13	0.561
Mdh	( + )	AsnS	asparaginyl-tRNA synthetase	49067.1	1	12	0.314
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	12	0.51
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	12	0.49
Mdh	( + )	PckA	phosphoenolpyruvate carboxykinase	58283.2	1	11	0.231
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	11	0.429
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	11	0.49
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	11	0.462
Mdh	( + )	PdxS	pyridoxal-5'-phosphate synthase	31593.3	1	11	0.405
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	10	0.417
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	10	0.484
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	10	0.468
Mdh	( + )	AroA	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase / chorismate mutase-isozyme 3	39522.5	1	10	0.316
Mdh	( + )	DapA	dihydrodipicolinate synthase	31024.3	1	10	0.538
Mdh	( + )	ThiG	hydroxyethylthiazole phosphate biosynthesis	27005.2	1	10	0.488
Mdh	( + )	DegU	two-component response regulator involved in degradative enzyme and competence regulation	25848.1	1	10	0.463
Mdh	( + )	TenA	transcriptional regulator of extracellular enzyme genes	27399	1	10	0.407
Mdh	( + )	GndA	NADP-dependent phosphogluconate dehydrogenase	51758.8	1	10	0.262
Mdh	( + )	PckA	phosphoenolpyruvate carboxykinase	58283.2	1	9	0.207
Mdh	( + )	AtpA	ATP synthase (subunit alpha)	54581.9	1	9	0.203
Mdh	( + )	SucC	succinyl-CoA synthetase (beta subunit)	41354.4	1	9	0.278
Mdh	( + )	Pgi	glucose-6-phosphate isomerase	50510.8	1	9	0.284
Mdh	( + )	TufA	elongation factor Tu	43574.9	1	9	0.328
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	9	0.407
Mdh	( + )	FbaA	fructose-1,6-bisphosphate aldolase	30382.3	1	9	0.309
Mdh	( + )	SerA	phosphoglycerate dehydrogenase	57098.2	1	8	0.179
Mdh	( + )	Zwf	probable glucose-6-phosphate 1-dehydrogenase	55643.4	1	8	0.258
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	8	0.333
Mdh	( + )	SufC	ABC transporter (ATP-binding protein), synthesis of Fe-S clusters	29014.3	1	8	0.441
Mdh	( + )	RpsB	ribosomal protein S2	27950.2	1	8	0.289
Mdh	( + )	GuaA	GMP synthetase	57934.6	1	7	0.177
Mdh	( + )	PurL	phosphoribosylformylglycinamidine synthetase II	80273.6	1	7	0.108
Mdh	( + )	YpwA	unknown; similar to carboxypeptidase	58159.5	1	7	0.164
Mdh	( + )	LeuA	2-isopropylmalate synthase	56894.6	1	7	0.193
Mdh	( + )	LutB	lactate oxidase	53456.7	1	7	0.167
Mdh	( + )	Ndh	NADH dehydrogenase	41935.8	1	7	0.227
Mdh	( + )	Spo0M	sporulation-control gene	29689.7	1	7	0.279
Mdh	( + )	Mdh	malate dehydrogenase	33626	1	7	0.359
Mdh	( + )	PepF	oligoendopeptidase	70128.7	1	6	0.133
Mdh	( + )	ArgS	arginyl-tRNA synthetase	62707.3	1	6	0.128
Mdh	( + )	SufB	synthesis of Fe-S-clusters	52712.8	1	6	0.142
Mdh	( + )	GltB	glutamate synthase (small subunit)	54931	1	6	0.168
Mdh	( + )	IlvC	ketol-acid reductoisomerase	37438.8	1	6	0.251
Mdh	( + )	Prs	phosphoribosylpyrophosphate synthetase	34851.5	1	6	0.249
Mdh	( + )	FabI	enoyl-acyl carrier protein reductase	27856.5	1	6	0.26
Mdh	( + )	GtaB	UTP-glucose-1-phosphate uridylyltransferase	33053.3	1	6	0.257
Mdh	( + )	DnaK	class I heat-shock protein (molecular chaperone)	65985	1	5	0.0966
Mdh	( + )	GroEL	class I heat-shock protein (chaperonin)	57406.3	1	5	0.101
Mdh	( + )	PdhC	pyruvate dehydrogenase (dihydrolipoamide acetyltransferase E2 subunit)	47520.6	1	5	0.138
Mdh	( + )	SufD	synthesis of Fe-S-clusters	48275.6	1	5	0.108
Mdh	( + )	RnjA	RNase J1	61500.7	1	5	0.142
Mdh	( + )	AccC	acetyl-CoA carboxylase (biotin carboxylase subunit)	49560.3	1	5	0.133
Mdh	( + )	OxdC	oxalate decarboxylase	43548.5	1	5	0.161
Mdh	( + )	TufA	elongation factor Tu	43574.9	1	5	0.167
Mdh	( + )	TufA	elongation factor Tu	43574.9	1	5	0.169
Mdh	( + )	CshA	DEAD-box RNA helicase	57262.1	1	5	0.129
Mdh	( + )	SerS	seryl-tRNA synthetase	48824.7	1	5	0.144
Mdh	( + )	AhpF	alkyl hydroperoxide reductase (large subunit) / NADH dehydrogenase	54856.4	1	5	0.0943
Mdh	( + )	GatB	glutamyl-tRNA(Gln) amidotransferase (subunit B)	53390.9	1	5	0.126
Mdh	( + )	CitZ	citrate synthase II (major)	41712.3	1	5	0.177
Mdh	( + )	GapA	glyceraldehyde-3-phosphate dehydrogenase	35814.5	1	5	0.182
Mdh	( + )	YumC	ferredoxin-NAD(P)+ oxidoreductase	36823.2	1	5	0.226
Mdh	( + )	Tsf	elongation factor Ts	32336	1	5	0.239
Mdh	( + )	ThrB	homoserine kinase	33306.7	1	5	0.168
Mdh	( + )	YkwC	putative beta-hydroxyacid dehydrogenase	30694.6	1	5	0.257
Mdh	( + )	YwfI	unknown	29488.3	1	5	0.169
Mdh	( + )	CitG	fumarate hydratase	50514.7	1	5	0.152
Mdh	( + )	RpsC	ribosomal protein S3 (BS3)	24315.5	1	5	0.22
Mdh	( + )	CitB	aconitate hydratase	99317.3	1	4	0.044
Mdh	( + )	AlaS	alanyl-tRNA synthetase	97262.1	1	4	0.0569
Mdh	( + )	PdhD	pyruvate dehydrogenase / 2-oxoglutarate dehydrogenase (dihydrolipoamide dehydrogenase E3 subunit)	49715.2	1	4	0.0936
Mdh	( + )	GltX	glutamyl-tRNA synthetase	55705.9	1	4	0.11
Mdh	( + )	FabF	beta-ketoacyl-acyl carrier protein synthase II	43987.6	1	4	0.104